Enriching for orthologs increases support for Xenacoelomorpha and Ambulacraria sister relationship

Conflicting studies place a group of bilaterian invertebrates containing xenoturbellids and acoelomorphs, the Xenacoelomorpha, as either the primary emerging bilaterian phylum, or within Deuterostomia, sister to Ambulacraria. While their placement as sister to the rest of Bilateria supports relatively simple morphology in the ancestral bilaterian, their alternative placement within Deuterostomia suggests a morphologically complex ancestral Bilaterian along with extensive loss of major phenotypic traits in the Xenacoelomorpha. More recently, further studies have brought into question whether Deuterostomia should be considered monophyletic at all. Hidden paralogy presents a major challenge for reconstructing species phylogenies. Here we assess whether hidden paralogy has contributed to the conflict over the placement of Xenacoelomorpha. Our approach assesses previously published datasets, enriching for orthogroups whose gene trees support well resolved clans elsewhere in the animal tree of life. We find that the majority of constituent genes in previously published datasets violate incontestable clans, suggesting that hidden paralogy is rife at this depth. We demonstrate that enrichment for genes with orthologous signal alters the final topology that is inferred, whilst simultaneously improving fit of the model to the data. We discover increased, but ultimately not conclusive, support for the existence of Xenambulacraria in our orthology enriched set of genes. At a time when we are steadily progressing towards sequencing all of life on the planet, we argue that long-standing contentious issues in the tree of life will be resolved using smaller amounts of better quality data that can be modelled adequately.

A COVID-19 Re-envisioned Teaching Practicum Curriculum

The changes taking place in the schooling landscape because of the coronavirus are real and meaningful and have implications for the training of preservice teachers, especially the teaching practicum component of their initial teacher education programs. The havoc caused by COVID-19 forced many faculties of education into a state of ‘panic-gogy’ to ensure that student teachers could complete their teaching practicums. In this paper we argue that consideration should be given to core teaching practices and practice-based teacher education pedagogies (i.e., representations and approximations) that can be used in alternative “placement contexts” that will supplement and harness the authenticity of school-based experiences. We provide two conceptual tools, collaboratively developed by teaching practicum educators and mentor teachers, that can be used as heuristic by other university-school partnerships when considering teaching practicum redesign efforts.

Enhancing Peer Learning through Online Placements for Health and Social Care Professions

This article reports on the innovative design and the delivery of an online placement for university students, in response to the suspension of face-to-face placements due to COVID-19 restrictions in April 2020. A high quality, evidence-based and pedagogically sound experience was built, delivered and reviewed. The online placement not only met the professional standards and demonstrated equivalence in achieving placement learning outcomes, compared to traditional face to face placements, but also offered emergent benefits. The online placement showed enhanced peer learning and improved professional reasoning development by the students. In view of the interest and need for alternative placements, online placements were explored further, contextualised and built into a model which came to be known as Peer-Enhanced E-Placement (PEEP). Throughout 2020, and into 2021, the ‘logjam’ of increased student numbers and reduced placements, dramatically increased the interest in alternative placement learning and the PEEP model. A PEEP professional development acquisition experience was developed and piloted, to disseminate, scale and contextualize the approach by health and social care teams. Feedback from the pilot acquisition experiences indicated that the participants were able to design and implement PEEPs in their own contexts with their students. The scaling up and roll out of the PEEP acquisition experiences now extends to over fifteen professions. Conclusions include that the PEEP model offers a viable, acceptable and successful online practice-based learning option for health and social care students, achieving equivalence in placement learning outcomes and enhanced peer learning.

283 Timely Euthanasia on Farm; Dairy Cattle and Swine

Euthanasia of mature swine and cattle can be challenging. On-farm euthanasia should be used as a tool to eliminate pain and suffering. However, clear guidelines regarding making euthanasia decisions and alternative euthanasia techniques available for use is limited in the United States (US). In order to prevent prolonged suffering and pain in compromised animals, science-based recommendations are needed to ensure timely and humane euthanasia can be performed when needed on-farm. This presentation will focus on two euthanasia challenges currently faced in swine and dairy cattle systems in the US today: 1) Swine: Validating alternative euthanasia techniques for use in mature breeding stock and 2) Dairy Cattle: Identifying producer barriers preventing timely euthanasia decision-making using surveys and focus groups. The swine study evaluated the effectiveness of two penetrating captive bolt gun styles (cylinder or pistol) using a frontal, temporal and behind-the-ear placement. Four treatments were 100% effective in achieving cardiac arrest and death. The cylinder style captive bolt gun resulted in greater brain trauma and death compared to a pistol style gun and behind-the-ear and temporal placement showed promise as an alternative placement site for euthanizing mature pigs. In the dairy cattle study, dairy producers were recruited to participate in a survey and focus group. Survey results indicated that farm owners were most commonly responsible for on-farm euthanasia and most respondents would treat and monitor compromised cattle for a majority of health conditions, regardless of condition severity. Participants in focus groups focused primarily on animal welfare as the most important factor influencing the decision to euthanize and the desire to eliminate animal suffering by using euthanasia as a tool. This work highlights the complicated challenges that arise when euthanizing livestock and the importance of not only identifying appropriate techniques to humanely euthanize livestock but address the emotional and animal welfare factors that influence these decisions.

Abstract P120: The Covid Effect on Stroke Response

Background & Significance: The Covid-19 pandemic has created a host of challenges for healthcare systems and hospital teams that have put unprecedented stress on staff and leaders to re-design care and management of not only the Covid positive patient but also the hospitalized non-Covid patient. As this large healthcare system began to prepare for a Covid surge of patients, stroke program leaders recognized the need for alternative placement and management plans. With the re-designation of units and beds and deployment of staff into non-primary units, program leaders were concerned with not only placement of stroke patients outside of regular stroke and Neurocritical Care Units but also with non-trained stroke nursing staff caring for the patient. In response, this stroke program convened a working group to create alternative guidelines for care of the stroke patient during Covid surge and critical bed shortages. Design & Methods: The need for established criteria to guide all sites in the care of patients post IV thrombolytic or mechanical thrombectomy was the key objective. The alternative guideline was drafted and submitted to the system Covid clinical command center for emergent approval. Once approval given, education was provided to all stroke coordinators and key leaders at each site. To provide full access, guidelines were posted and available on the system SharePoint site for access to all team members. Results: The drafting of alternative stroke guidelines allowed for improved patient safety during our 27-hospital healthcare systems Covid surge. Care of multiple patients occurred outside normal critical care and stroke units with a decreased number of assessments for patients from the standard, pre-Covid and without increase safety events or adverse outcomes. The success of the alternative guidelines and this Covid effect on stroke care management not only helped our staff and patients during a time of need in safe care but also provided a new model of care for our stroke program leaders to consider and implement across our organizations.

Validating the effectiveness of alternative euthanasia techniques using penetrating captive bolt guns in mature swine (Sus scrofa domesticus)

ABSTRACT: Euthanasia of mature swine is challenging. Temporal and behind-the-ear locations are two sites that have been identified as alternatives to the more commonly used frontal placement. In stage-one, the effectiveness of two penetrating captive bolt gun styles (cylinder or pistol) was evaluated using frontal, temporal and behind-the-ear placement in anesthetized mature swine (n=36; weight: 267 ± 41 kg). For stage-one, when evaluating treatment efficacy by sex, the cylinder style equipment was 100% effective in achieving death when applied to all cranial locations (frontal, temporal, behind-the-ear) for sows; however, the pistol style equipment was only 100% effective when applied at the behind-the-ear location for sows. For boars, the cylinder style equipment was 100% effective when applied to the frontal and behind-the-ear location, but the pistol style equipment was not effective for any cranial location in boars. Therefore, the pistol-frontal, pistol-temporal, pistol- behind-the-ear and cylinder-temporal were not included for boars, and pistol-frontal and pistol-temporal were not included for sows in stage-two. In stage-two, commercial, mixed breed, mature swine (n=42; weight: 292 +/- 56 kg) were randomly assigned to one of four treatments based on inclusion criteria described in stage-one. A 3-point traumatic brain injury (TBI) score (0=normal; 1=some abnormalities; 3=grossly abnormal, unrecognizable) was used to evaluate six neuroanatomical structures (cerebral cortex, cerebellum, hypothalamus, thalamus, pons and brain stem) and the presence of hemorrhage was also noted. All treatments were 100% effective in stage-two. A significant interaction between gun style and placement was determined on predicting total TBI as the cylinder style produced a higher total TBI score compared to the pistol type, of magnitude of +2.8 (P < 0.01). The cylinder style tended to produce a greater TBI score than the pistol in the temporal location (+1.2; P=0.08). No difference was noted for TBI score behind-the-ear between the cylinder and pistol style gun (P>0.05). TBI tended to be less in boars compared to sows (-0.6; P=0.08). Hemorrhage was observed in frontal, parietal, occipital and temporal lobes. This study demonstrated that the cylinder style captive bolt gun more effectively resulted in brain trauma and death compared to a pistol style gun and the behind-the-ear and temporal placement showed promise as an alternative placement site for euthanizing mature pigs on-farm.

An alternative placement model for nursing students: discovering new horizons

This article explores the use of an alternative placement model in conjunction with the Nursing and Midwifery Council standards for education and training. Traditionally in nurse education, students were supported on placement on a one-to-one basis by a qualified nurse mentor. This could be a very intense relationship and could limit placement learning as students were only allocated to areas that have a qualified nurse mentor, exacerbating competing demands on placement capacity to support students. A higher education institute trialled an alternative placement model that used several healthcare-related services traditionally not used for nursing placements. Some of these placements were allocated by the higher education institute, but students were also responsible for securing a number of placements for themselves. The students were supported with their learning by appropriate healthcare staff in practice but were assessed by academic members of staff at the higher education institute acting as practice assessors. The project was evaluated positively overall by the vast majority of students and staff. Students found it to be an empowering experience, which encouraged autonomous practice.

Analysis of Signage using Eye-Tracking Technology

Signs, in all their forms and manifestations, provide visual communication for wayfinding, commerce, and public dialogue and expression. Yet, how effectively a sign communicates and ultimately elicits a desired reaction begins with how well it attracts the visual attention of prospective viewers. This is especially the case for complex visual environments, both outside and inside of buildings. This paper presents the results of an exploratory research design to assess the use of eye-tracking (ET) technology to explore how placement and context affect the capture of visual attention. Specifically, this research explores the use of ET hardware and software in real-world contexts to analyze how visual attention is impacted by location and proximity to geometric edges, as well as elements of contrast, intensity against context, and facial features. Researchers also used data visualization and interpretation tools in augmented reality environments to anticipate human responses to alternative placement and design. Results show that ET methods, supported by the screen-based and wearable eye-tracking technologies, can provide results that are consistent with previous research of signage performance using static images in terms of cognitive load and legibility, and ET technologies offer an advanced dynamic tool for the design and placement of signage.

Health-Care Professionals Perceived Barriers to Effective Cancer Pain Management in the Home Hospice Setting: Is Dying at Home Really Best?

Hospice health-care professionals (HCP) evaluate and manage cancer pain in patient homes. This study explores HCP’s perceptions of barriers that affect pain management for home hospice cancer patients. A convenience sample of 20 experienced hospice HCP were recruited from a regional hospice agency. Data were collected through two focus groups using semistructured interviews and analyzed using a constant comparative approach to generate themes. An unexpected finding revealed patient’s religious and cultural beliefs about suffering and family caregiver’s beliefs that patients deserve to suffer due to past actions are barriers to pain management in home hospice. Hospice HCP can identify patients at risk for suffering at the end of life. Interventions targeting spiritual suffering and needs are needed. Home hospice HCP have an ethical obligation to address undue suffering through family’s withholding of necessary pain medications and should consider alternative placement when home is not suitable for a peaceful death.

Battery Sizing for Electric Vehicles Based on Real Driving Patterns in Thailand

The rising population in suburban areas have led to an increasing demand for commuter buses. Coupled with a desire to reduce pollution from the daily routine of traveling and transportation, electric vehicles have become more interesting as an alternative placement for internal combustion engine vehicles. However, in comparison to those conventional vehicles, electric vehicles have an issue of limited driving range. One of the main challenges in designing electric vehicles (EVs) is to estimate the size and power of energy storage system, i.e., battery pack, for any specific application. Reliable information on energy consumption of vehicle of interest is therefore necessary for a successful EV implementation in terms of both performance and cost. However, energy consumption usually depends on several factors such as traffic conditions, driving cycle, velocities, road topology, etc. This paper presents an energy consumption analysis of electric vehicle in three different route types i.e., closed-area, inter-city, and local feeder operated by campus tram and shuttle bus. The driving data of NGV campus trams operating in a university located in suburban Bangkok and that of shuttle buses operating between local areas and en route to the city were collected and the corresponding representative driving cycles for each route were generated. The purpose of this study was to carry out a battery sizing based on the fulfilment of power requirements from the representative real driving pattern in Thailand. The real driving cycle data i.e., velocity and vehicle global position were collected through a GPS-based piece of equipment, VBOX. Three campus driving data types were gathered to achieve a suitable dimensioning of battery systems for electrified university public buses.