Aspergillus oryzae Grown on Rice Hulls Used as an Additive for Pretreatment of Starch-Containing Wastewater from the Pulp and Paper Industry

From an industrial point of view, the use of microorganisms as a wastewater bioremediation practice represents a sustainable and economic alternative for conventional treatments. In this work, we investigated the starch bioremediation of paper mill wastewater (PMW) with Aspergillus oryzae. This amylase-producing fungus was tested in submerged fermentation technology (SmF) and solid-state fermentation (SSF) on rice hulls. The tests were conducted to assay the concentration of the reducing sugars on paper mill wastewater. The bioremediation of starch in the wastewater was carried out by A. oryzae, which proved capable of growing in this complex media as well as expressing its amylase activity.

Similar Methanogenic Shift but Divergent Syntrophic Partners in Anaerobic Digesters Exposed to Direct versus Successive Ammonium Additions

Anaerobic digestion (AD) is an attractive biotechnological process for wastewater bioremediation and bioenergy production in the form of methane-rich biogas. However, AD can be inhibited by ammonium generated by protein-rich effluent, commonly found in agro-industrial activities.

Potential of Microalgae in Bioremediation of Wastewater

The increase in global pollution, industrialization and fast economic progress are considered to inflict serious consequences to the quality and availability of water throughout the world. Wastewater is generated from three major sources, i.e. industrial, agricultural, and municipal which contain pollutants, such as: xenobiotics, microplastics, heavy metals and augmented by high amount of carbon, phosphorus, and nitrogen compounds. Wastewater treatment is one of the most pressing issues since it cannot be achieved by any specific technology because of the varying nature and concentrations of pollutants and efficiency of the treatment technologies. The degradation capacity of these conventional treatment technologies is limited, especially regarding heavy metals, nutrients, and xenobiotics, steering the researchers to bioremediation using microalgae (Phycoremediation). Bioremediation can be defined as use of microalgae for removal or biotransformation of pollutants and CO2 from wastewater with concomitant biomass production. However, the usage of wastewaters for the bulk cultivation of microalgae is advantageous for reducing carbon, nutrients cost, minimizing the consumption of freshwater, nitrogen, phosphorus recovery, and removal of other pollutants from wastewater and producing sufficient biomass for value addition for either biofuels or other value-added compounds. Several types of microalgae like Chlorella and Dunaliella have proved their applicability in the treatment of wastewaters. The bottlenecks concerning the microalgal wastewater bioremediation need to be identified and elucidated to proceed in bioremediation using microalgae. This objective of this paper is to provide an insight about the treatment of different wastewaters using microalgae and microalgal potential in the treatment of wastewaters containing heavy metals and emerging contaminants, with the specialized cultivation systems. This review also summarizes the end use applications of microalgal biomass which makes the bioremediation aspect more environmentally sustainable. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Immobilising Microalgae and Cyanobacteria as Biocomposites: New Opportunities to Intensify Algae Biotechnology and Bioprocessing

There is a groundswell of interest in applying phototrophic microorganisms, specifically microalgae and cyanobacteria, for biotechnology and ecosystem service applications. However, there are inherent challenges associated with conventional routes to their deployment (using ponds, raceways and photobioreactors) which are synonymous with suspension cultivation techniques. Cultivation as biofilms partly ameliorates these issues; however, based on the principles of process intensification, by taking a step beyond biofilms and exploiting nature inspired artificial cell immobilisation, new opportunities become available, particularly for applications requiring extensive deployment periods (e.g., carbon capture and wastewater bioremediation). We explore the rationale for, and approaches to immobilised cultivation, in particular the application of latex-based polymer immobilisation as living biocomposites. We discuss how biocomposites can be optimised at the design stage based on mass transfer limitations. Finally, we predict that biocomposites will have a defining role in realising the deployment of metabolically engineered organisms for real world applications that may tip the balance of risk towards their environmental deployment.