Structure Optimization of Semi-Solid Die Cast Steering Knuckle and its Experiment Verification

Steering knuckles are vital functional and structural components in automotive suspension systems, requiring high strength, high ductility, and complex shapes. In this study, an aluminum alloy knuckle with the semi-solid die casting process was developed to replace the conventional steel components. This research aims to optimize product design based on both structural simulation and casting process simulation to avoid defects and to meet mechanical requirements. Furthermore, the optimal design solutions need to be verified through the filling experiments and defect analysis. The results show that the removal of support rib located in the thick area of the shock absorber mounting arm is helpful to avoid the rewelding defects in the filling frontier of the SSM melt. Besides, the position of the steering rod is of medium thickness, and two ribs from different directions come together to support that area. Rewelding defects were detected when two ribs come together. To avoid rewelding defects in local areas of steering rod position, the ribs were reduced to uniform wall thickness. Thus, the local flow state was modified and the SSM melt was reinforced shear action. Ultimately, by controlling all the processes of the SSM die casting process, the high performance of aluminum knuckle was successfully developed.

The Ground-Based BIOMEX Experiment Verification Tests for Life Detection on Mars

The success of an astrobiological search for life campaign on Mars, or other planetary bodies in the Solar System, relies on the detectability of past or present microbial life traces, namely, biosignatures. Spectroscopic methods require little or no sample preparation, can be repeated almost endlessly, and can be performed in contact or even remotely. Such methods are therefore ideally suited to use for the detection of biosignatures, which can be confirmed with supporting instrumentation. Here, we discuss the use of Raman and Fourier Transform Infrared (FT-IR) spectroscopies for the detection and characterization of biosignatures from colonies of the fungus Cryomyces antarcticus, grown on Martian analogues and exposed to increasing doses of UV irradiation under dried conditions. The results report significant UV-induced DNA damage, but the non-exceeding of thresholds for allowing DNA amplification and detection, while the spectral properties of the fungal melanin remained unaltered, and pigment detection and identification was achieved via complementary analytical techniques. Finally, this work found that fungal cell wall compounds, likely chitin, were not degraded, and were still detectable even after high UV irradiation doses. The implications for the preservation and detection of biosignatures in extraterrestrial environments are discussed.

On the Study of Configuration and Kinematics of a 3-DOF Generalized Spherical Parallel Mechanism for Ankle Rehabilitation

The kinematic equivalent model of the existing ankle rehabilitation robot is inconsistent with the anatomy structure of the human ankle, which will influence the rehabilitation effect . Therefore, this paper equivalent the human ankle to the UR model and proposes a novel 3-DOF generalized spherical parallel mechanism for ankle rehabilitation. The parallel mechanism has two spherical centers corresponding to the rotation center of the tibiotalar joint and subtalar joint. Via screw theory, the mobility of the parallel mechanism is analyzed, which meets the requirement of the human ankle. Its inverse kinematics is presented and singularities are identified based on the Jacobian matrix. The workspaces of the parallel mechanism are obtained by the search method and compared with the motion range of the human ankle, which shows that the parallel mechanism could meet the motion demand of ankle rehabilitation. In addition, on the basis of the motion/force transmissibility, the performance atlases are plotted in the parameter optimal design space and the optimum region is obtained according to the demands of practical application. The results show that the parallel mechanism can meet the motion requirements of ankle rehabilitation and have excellent kinematic performance in its rehabilitation range, which provides a theoretical basis for the prototype design and experiment verification.

Uncovering the Mechanism of Curcuma in the Treatment of Ulcerative Colitis Based on Network Pharmacology, Molecular Docking Technology, and Experiment Verification

Aim. The incidence of ulcerative colitis (UC) is increasing steadily in developed countries, it is plaguing nearly 1 million people in the United States and European countries, while developing countries have had a rapidly increased incidence over the past decades. Curcuma is widely used in treating malaria, UC, Crohn’s disease, and colon cancer, which lead to diarrhea and bloody stool. However, the systemic mechanism of curcuma in treating UC is still unclear. Our work was supposed to expound how does curcuma alleviate UC in a comprehensive and systematic way by network pharmacology, molecular docking, and experiment verification. Methods. Traditional Chinese Medicine System Pharmacology Database (TCMSP), Shanghai Chemistry & Chemical Industry Data Platform (SGST), and papers published in Chinese Network Knowledge Infrastructure (CNKI) and PubMed were used to collect the chemical constituents of curcuma based on ADME (absorption, distribution, metabolism, and excretion). And effective targets were predicted by Swiss Target Prediction to establish the curcuma-related database. The disease targets of UC were screened by GeneCards and DrugBank databases, and Wayne (Venn) analysis was carried out with curcuma targets to determine the intersection targets. AutoDock software and TCMNPAS system were used to dock the core chemical components of curcuma with key UC targets. Protein interaction (PPI) network was constructed based on the STRING database and Cytoscape software. Gene function GO analysis and KEGG pathway enrichment analysis were carried out by using Metascape database. Finally, HE staining was performed to identify the inflammatory infiltration and expression difference in TNF-α and STAT3 before and after the treatment of curcuma which was verified by immunoblotting. Results. Twelve active components containing 148 target genes were selected from curcuma. Potential therapeutic targets of curcuma in the treatment of UC were acquired from 54 overlapped targets from UC and curcuma. Molecular docking was used to filter the exact 24 core proteins interacting with compounds whose docking energy is lower than −5.5 and stronger than that of 5-aminosalicylic acid (5-ASA). GO and KEGG analyses showed that these targets were highly correlated with EGFR tyrosine kinase inhibitor resistance, PI3K-Akt signaling pathway, JAK-STAT signaling pathway, MAPK signaling pathway, and inflammatory bowel disease (IBD). Experiments verified curcuma relieved pathological manifestation and decreased the expression of TNF-α and STAT3. Conclusion. Curcuma relieved the colon inflammation of ulcerative colitis via inactivating TNF pathway, inflammatory bowel disease pathway, and epithelial cell signaling in Helicobacter pylori infection pathway, probably by binding to STAT3 and TNF-α.