Rapid, Simple and Inexpensive Fabrication of Paper-based Analytical Devices by Parafilm® Hot Pressing

2021 ◽  
Author(s):  
Sursak Kasetsirikul ◽  
Kimberley Clack ◽  
Muhammad J.A. Shiddiky ◽  
Nam-Trung Nguyen
Keyword(s):  
Hot Pressing ◽  
Binding Capacity ◽  
Three Dimensional ◽  
Biochemical Properties ◽  
Biomolecule Detection ◽  
Pressing Time ◽  
Resource Limited ◽  
Hydrophobic Barrier ◽  
Protein Binding Capacity ◽  
Paper Device

Paper-based analytical devices have been substantially developed in recent decades. Many fabrication techniques for paper-based analytical devices have been demonstrated and reported. Herein we report a relatively rapid, simple, and inexpensive method for fabricating paper-based analytical devices using parafilm hot pressing. We studied and optimized the effect of the key fabrication parameters, namely pressure, temperature, and pressing time. We discerned the optimal conditions, including pressure of 3.8 MPa (3 tons), temperature of 80oC, and 3 minutes of pressing time, with the smallest hydrophobic barrier size (821 µm) being governed by laminate mask and parafilm dispersal from pressure and heat. Physical and biochemical properties were evaluated to substantiate the paper functionality for analytical devices. Wicking speed in the fabricated paper strips was slightly slower than that of non-processed paper, resulting from reducing paper pore size. A colorimetric immunological assay was performed to demonstrate the protein binding capacity of the paper-based device after exposure to pressure and heat from the fabrication. Moreover, mixing in two-dimensional paper-based device and flowing in a three-dimensional counterpart were thoroughly investigated, demonstrating that the paper device from this fabrication process is potentially applicable as analytical devices for biomolecule detection. Fast, easy, and inexpensive parafilm hot press fabrication presents an opportunity for researchers to develop paper-based analytical devices in resource-limited environments.

scholarly journals Rapid, Simple and Inexpensive Fabrication of Paper-Based Analytical Devices by Parafilm® Hot Pressing

Micromachines ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 48
Author(s):  
Surasak Kasetsirikul ◽  
Kimberley Clack ◽  
Muhammad J. A. Shiddiky ◽  
Nam-Trung Nguyen
Keyword(s):  
Hot Pressing ◽  
Binding Capacity ◽  
Three Dimensional ◽  
Biochemical Properties ◽  
Hot Press ◽  
Biomolecule Detection ◽  
Pressing Time ◽  
Resource Limited ◽  
Hydrophobic Barrier ◽  
Protein Binding Capacity

Paper-based analytical devices have been substantially developed in recent decades. Many fabrication techniques for paper-based analytical devices have been demonstrated and reported. Herein, we report a relatively rapid, simple, and inexpensive method for fabricating paper-based analytical devices using parafilm hot pressing. We studied and optimized the effect of the key fabrication parameters, namely pressure, temperature, and pressing time. We discerned the optimal conditions, including a pressure of 3.8 MPa, temperature of 80 °C, and 3 min of pressing time, with the smallest hydrophobic barrier size (821 µm) being governed by laminate mask and parafilm dispersal from pressure and heat. Physical and biochemical properties were evaluated to substantiate the paper functionality for analytical devices. The wicking speed in the fabricated paper strips was slightly lower than that of non-processed paper, resulting from a reduced paper pore size after hot pressing. A colorimetric immunological assay was performed to demonstrate the protein binding capacity of the paper-based device after exposure to pressure and heat from the fabrication. Moreover, mixing in a two-dimensional paper-based device and flowing in a three-dimensional counterpart were thoroughly investigated, demonstrating that the paper devices from this fabrication process are potentially applicable as analytical devices for biomolecule detection. Fast, easy, and inexpensive parafilm hot press fabrication presents an opportunity for researchers to develop paper-based analytical devices in resource-limited environments.

scholarly journals Structure and characteristics of the hot pressed hydroxyapatite/poly-L-lactide composite

2002 ◽  
Vol 34 (1) ◽  
pp. 79-93 ◽  
Author(s):  
Nenad Ignjatovic ◽  
Edin Suljovrujic ◽  
Z. Stojanovic ◽  
Dragan Uskokovic
Keyword(s):  
Hot Pressing ◽  
Three Dimensional ◽  
Dispersion Analysis ◽  
Degree Of Crystallinity ◽  
Time Interval ◽  
Scanning Calorimetry ◽  
Pressing Time ◽  
Ir Study ◽  
Electron Dispersion ◽  
The Degree Of Crystallinity

Hydroxyapatite/poly-L-lactide (HAp/PLL) composite biomaterial can be obtained by different processing methods. Three-dimensional blocks of HAp/PLLA composite biomaterial with mechanical characteristics close to the natural bone tissue can be obtained by hot pressing procedure. Effects of synthesis and compacting on the structure and characteristics of the HAp/PLLA composite biomaterial were studied in this work. Using wade angle X-ray structural analyses (WAXS), differentially scanning calorimetry (DSC), thermogravimetric analysis (TGA) and infrared (IR) spectroscopy, the changes occurring in the material during synthesis and hot pressing were monitored. Surface microstructure was analyzed by scanning electronic microscopy (SEM) coupled with electron-dispersion analysis (EDX). The results obtained indicate a possible decrease in the degree of crystallinity with hot pressing time increase. A block of HAp/PLLA composite biomaterial with 1.6 times lower crystallinity of the polymer phase was obtained by hot pressing in a given time interval with a maximum of 60 minutes. Results of TG analysis show that PLLA stability decreases with increasing hot pressing time, and vice versa. IR study proved that neither destructive changes in constituents nor formation of new phases occurred during hot pressing.

Modeling and Simulation of Optimal Blank Design and Hot Pressing Process for Manufacturing Large Francis Turbines Blades From Very Thick Plates

2013 ◽  
Author(s):  
Zhengkun Feng ◽  
Henri Champliaud ◽  
Louis Mathieu ◽  
Michel Sabourin
Keyword(s):  
Hot Pressing ◽  
Three Dimensional ◽  
High Strength ◽  
Production Costs ◽  
Francis Turbine ◽  
Thick Plates ◽  
Pressing Process ◽  
Blank Design ◽  
Cost Of Energy ◽  
Applied Forces

Hot pressing process is widely used in automotive, shipbuilding, energy production and civil engineering. However, the trial and error technique that is intensive time and energy consuming is still used. Particularly, the design of Francis turbines of hydropower plants is not standard, but variable from site to site due to hydraulic conditions and cost of energy. As a result, the blade hydraulic profile of each Francis turbine is different. The blades, one of the key components of Francis turbine runners, are produced in small batches and the setup of the dedicated punch and die increases significantly the unit production costs. In this paper, the blade unfolding process for optimal blank design will be firstly presented, and then a hot pressing process for very thick plates is proposed. The pressing process of high strength steel at hot temperature is characterized by thermo-mechanical behaviors, three-dimensional unsteady deformation, high nonlinearity, continuous local forming. The analyses of residual stress distribution and applied forces are carried out.

scholarly journals High light induces species specific changes in the membrane lipid composition of Chlorella

2020 ◽  
Vol 477 (13) ◽  
pp. 2543-2559
Author(s):  
Janka Widzgowski ◽  
Alexander Vogel ◽  
Lena Altrogge ◽  
Julia Pfaff ◽  
Heiko Schoof ◽  
...  
Keyword(s):  
Cell Membranes ◽  
De Novo ◽  
Algal Cell ◽  
Isotopic Labeling ◽  
Biochemical Properties ◽  
Membrane Lipid ◽  
Light Conditions ◽  
Hydrophobic Barrier ◽  
Glycerolipid Composition ◽  
Species Specific

Algae have evolved several mechanisms to adjust to changing environmental conditions. To separate from their surroundings, algal cell membranes form a hydrophobic barrier that is critical for life. Thus, it is important to maintain or adjust the physical and biochemical properties of cell membranes which are exposed to environmental factors. Especially glycerolipids of thylakoid membranes, the site of photosynthesis and photoprotection within chloroplasts, are affected by different light conditions. Since little is known about membrane lipid remodeling upon different light treatments, we examined light induced alterations in the glycerolipid composition of the two Chlorella species, C. vulgaris and C. sorokiniana, which differ strongly in their ability to cope with different light intensities. Lipidomic analysis and isotopic labeling experiments revealed differences in the composition of their galactolipid species, although both species likely utilize galactolipid precursors originated from the endoplasmic reticulum. However, in silico research of de novo sequenced genomes and ortholog mapping of proteins putatively involved in lipid metabolism showed largely conserved lipid biosynthesis pathways suggesting species specific lipid remodeling mechanisms, which possibly have an impact on the response to different light conditions.

scholarly journals On the utilization of novel spectral laser scanning for three-dimensional classification of vegetation elements

2018 ◽  
Vol 8 (2) ◽  
pp. 20170039 ◽  
Author(s):  
Zhan Li ◽  
Michael Schaefer ◽  
Alan Strahler ◽  
Crystal Schaaf ◽  
David Jupp
Keyword(s):  
Laser Scanning ◽  
Spatial Information ◽  
Information Source ◽  
Three Dimensional ◽  
Laser Scanner ◽  
Biochemical Properties ◽  
Dual Wavelength ◽  
Wavelength Scanning ◽  
Spatial Attributes

The Dual-Wavelength Echidna Lidar (DWEL), a full waveform terrestrial laser scanner (TLS), has been used to scan a variety of forested and agricultural environments. From these scanning campaigns, we summarize the benefits and challenges given by DWEL's novel coaxial dual-wavelength scanning technology, particularly for the three-dimensional (3D) classification of vegetation elements. Simultaneous scanning at both 1064 nm and 1548 nm by DWEL instruments provides a new spectral dimension to TLS data that joins the 3D spatial dimension of lidar as an information source. Our point cloud classification algorithm explores the utilization of both spectral and spatial attributes of individual points from DWEL scans and highlights the strengths and weaknesses of each attribute domain. The spectral and spatial attributes for vegetation element classification each perform better in different parts of vegetation (canopy interior, fine branches, coarse trunks, etc.) and under different vegetation conditions (dead or live, leaf-on or leaf-off, water content, etc.). These environmental characteristics of vegetation, convolved with the lidar instrument specifications and lidar data quality, result in the actual capabilities of spectral and spatial attributes to classify vegetation elements in 3D space. The spectral and spatial information domains thus complement each other in the classification process. The joint use of both not only enhances the classification accuracy but also reduces its variance across the multiple vegetation types we have examined, highlighting the value of the DWEL as a new source of 3D spectral information. Wider deployment of the DWEL instruments is in practice currently held back by challenges in instrument development and the demands of data processing required by coaxial dual- or multi-wavelength scanning. But the simultaneous 3D acquisition of both spectral and spatial features, offered by new multispectral scanning instruments such as the DWEL, opens doors to study biophysical and biochemical properties of forested and agricultural ecosystems at more detailed scales.

scholarly journals Environmentally-Friendly High-Density Polyethylene-Bonded Plywood Panels

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1166 ◽  
Author(s):  
Pavlo Bekhta ◽  
Ján Sedliačik
Keyword(s):  
Mechanical Properties ◽  
Hot Pressing ◽  
High Density Polyethylene ◽  
Phenol Formaldehyde ◽  
Urea Formaldehyde ◽  
Physical And Mechanical Properties ◽  
Core Layer ◽  
High Density ◽  
Pressing Pressure ◽  
Pressing Time

Thermoplastic films exhibit good potential to be used as adhesives for the production of veneer-based composites. This work presents the first effort to develop and evaluate composites based on alder veneers and high-density polyethylene (HDPE) film. The effects of hot-pressing temperature (140, 160, and 180 °C), hot-pressing pressure (0.8, 1.2, and 1.6 MPa), hot-pressing time (1, 2, 3, and 5 min), and type of adhesives on the physical and mechanical properties of alder plywood panels were investigated. The effects of these variables on the core-layer temperature during the hot pressing of multiplywood panels using various adhesives were also studied. Three types of adhesives were used: urea–formaldehyde (UF), phenol–formaldehyde (PF), and HDPE film. UF and PF adhesives were used for the comparison. The findings of this work indicate that formaldehyde-free HDPE film adhesive gave values of mechanical properties of alder plywood panels that are comparable to those obtained with traditional UF and PF adhesives, even though the adhesive dosage and pressing pressure were lower than when UF and PF adhesives were used. The obtained bonding strength values of HDPE-bonded alder plywood panels ranged from 0.74 to 2.38 MPa and met the European Standard EN 314-2 for Class 1 plywood. The optimum conditions for the bonding of HDPE plywood were 160 °C, 0.8 MPa, and 3 min.

Sugarcane (Saccharum officinarium L.) bagasse binderless particleboard: Effect of hot pressing time study

2020 ◽  
Vol 31 ◽  
pp. 313-317
Author(s):  
Wan Noor Aidawati Wan Nadhari ◽  
Norani Abd Karim ◽  
Jia Geng Boon ◽  
Kushairi Mohd Salleh ◽  
Asniza Mustapha ◽  
...  
Keyword(s):  
Hot Pressing ◽  
Time Study ◽  
Pressing Time

scholarly journals Impact of Silanization Parameters and Antibody Immobilization Strategy on Binding Capacity of Photonic Ring Resonators

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3163
Author(s):  
Nina Bjørk Arnfinnsdottir ◽  
Cole A. Chapman ◽  
Ryan C. Bailey ◽  
Astrid Aksnes ◽  
Bjørn Torger Stokke
Keyword(s):  
Protein Binding ◽  
Ring Resonator ◽  
Silicon Oxide ◽  
Binding Capacity ◽  
Ring Resonators ◽  
Widespread Application ◽  
Reactive Protein ◽  
Oxide Substrates ◽  
Protein Binding Capacity ◽  
Selection Of

Ring resonator-based biosensors have found widespread application as the transducing principle in “lab-on-a-chip” platforms due to their sensitivity, small size and support for multiplexed sensing. Their sensitivity is, however, not inherently selective towards biomarkers, and surface functionalization of the sensors is key in transforming the sensitivity to be specific for a particular biomarker. There is currently no consensus on process parameters for optimized functionalization of these sensors. Moreover, the procedures are typically optimized on flat silicon oxide substrates as test systems prior to applying the procedure to the actual sensor. Here we present what is, to our knowledge, the first comparison of optimization of silanization on flat silicon oxide substrates to results of protein capture on sensors where all parameters of two conjugation protocols are tested on both platforms. The conjugation protocols differed in the chosen silanization solvents and protein immobilization strategy. The data show that selection of acetic acid as the solvent in the silanization step generally yields a higher protein binding capacity for C-reactive protein (CRP) onto anti-CRP functionalized ring resonator sensors than using ethanol as the solvent. Furthermore, using the BS3 linker resulted in more consistent protein binding capacity across the silanization parameters tested. Overall, the data indicate that selection of parameters in the silanization and immobilization protocols harbor potential for improved biosensor binding capacity and should therefore be included as an essential part of the biosensor development process.

Physical Performance of Bamboo Strips-Oil Palm Trunk Veneers Composite Lumber at Different Layer Orientations and Hot Pressing Times

2012 ◽  
Vol 545 ◽  
pp. 325-329 ◽  
Author(s):  
Wan Nor Raihan Wan Jaafar ◽  
Shahril Anuar Bahari
Keyword(s):  
Physical Performance ◽  
Oil Palm ◽  
Hot Pressing ◽  
Cold Water ◽  
Phenol Formaldehyde ◽  
Hot Water ◽  
Compression Performance ◽  
Oil Palm Trunk ◽  
Pressing Time ◽  
Composite Lumber

In this study, composite lumber from a combination of bamboo strips and oil palm trunk veneers was produced and physically tested. The bamboo strips and oil palm trunk veneers were laid-up together alternately with two different types of layer orientation, such as parallel and cross orientations to each other. The composite lumber was pressed using hydraulic hot pressing machine at two different pressing times, such as 12 and 15 minutes. Phenol formaldehyde (PF) adhesive was used in the lamination process. Physical performance tests such as cold water delamination (CWD), hot water delamination (HWD), flexural and compression were conducted based on Japanese Agricultural Standard for LVL JAS: SE-10 [1]. Results showed that longer pressing time has increased the physical performance, except for flexural and compression performance of parallel orientation composite lumber. Cross orientation has increased the bonding strength behaviour between bamboo strips and oil palm trunk veneers, thus influenced the low delamination percentage and good modulus of elasticity value of composite lumber. Generally, this study has increased the understanding on physical performance of bamboo strips-oil palm trunk veneers composite lumber at different layer orientations and hot pressing times.

The Technology on Producing the First Class Plywood Using Fast Growing Eucalyptus Timber

2012 ◽  
Vol 193-194 ◽  
pp. 496-499 ◽  
Author(s):  
Kai Fu Li ◽  
Kai Meng Xu
Keyword(s):  
Production Technology ◽  
Hot Pressing ◽  
Bonding Strength ◽  
Key Factors ◽  
Pressing Temperature ◽  
Fast Growing ◽  
Pressing Time

There were three key factors including glue quantity of veneer, hot-pressing time, hot-pressing temperature to be researched on the effect of plywood performance, which gave a effective conclusion about technology parameter. The results indicated that the three factors obviously were related to the bonding strength and ranked as hot-pressing time > glue quantity of veneer > hot-pressing temperature in sequence. The best groups for production technology were that glue quantity of veneer was 320 g/m2 with hot-pressing time and temperature of 1.2 mm/min and 135 °C respectively.

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