Urban Forest Ecosystem Services Vary with Land Use and Species: A Case Study of Kyoto City

The demand for urban ecosystem services increases with the rapid growth of the urban population. The urban forest is a crucial ecosystem services provider in cities. To achieve a better estimation of urban ecosystem services, an understanding of the link between heterogeneity and ecosystem services within cities is needed. Other than street trees and forest remnants, the contribution of dispersed green spaces should also be considered. In this study, a ground-based sample quadrat investigation of trees across a sequence of land types in Kyoto City was applied. The ecosystem services and monetary values of trees were further calculated using a customized i-Tree Eco tool. The ecosystem services calculated include carbon storage and sequestration, air pollutants removal, and runoff reduction. Ecosystem services of different land use classes were compared at both quadrat and single-tree levels. We found no significant difference across land use for all the ecosystem services at the quadrat level. However, ecosystem services were different across land use at the single-tree level. We performed a species-specific analysis and found that the pattern of ecosystem services at the single-tree level across land use varies with both the service tested and species. Our study suggests that the heterogeneity within a city should be considered when estimating urban ecosystem services. The results also provide insight into the urban green space management of Kyoto City.

Responses of Photosynthesis And Long-Term Water Use Efficiency To Ambient Air Pollution In Urban Roadside Trees

We conducted on-site studies in Kyoto City, Japan, to evaluate the effect of air pollution by automobile gas exhaust on the leaf photosynthetic functions of four urban roadside tree species. Nitrogen oxides (NO and NO2) are major air pollutants that are related to automobile gas exhaust. The species-specific response of leaf photosynthesis to air pollution was obtained for single-year data, in which at the high air pollution sites, Rhododendron × pulchrum, Rhaphiolepis indica, and Prunus × yedoensis had a higher restriction of maximum photosynthesis (Amax), while the opposite trend was obtained for Ginkgo biloba. When the data were pooled across the years from 2007 to 2019 in R. pulchrum, the dose-dependent effect of NO and NO2 on photosynthesis became obvious, in which they decreased Amax and increased the long-term leaf water use efficiency. A spatial variability map for R. pulchrum showed a lower Amax and higher water use efficiency at the heavy traffic areas in Kyoto City, which suggests that R. pulchrum increased tolerance to air pollution and water stress at the expense of the leaf photosynthesis. This study revealed the importance of the evaluation of the species-specific response of photosynthesis to air pollution for the efficient use of urban trees, even in regions with relatively low atmospheric pollution levels such as < 40 ppb of NO or NO2.

Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis

In order to explore the role of exosomal circRNAs in the occurrence and development of sepsis, we looked for potential diagnostic markers to accurately identify sepsis and to lay a molecular basis for precise treatment. Ultracentrifugation was used to extract exosomes from the serum of patients with sepsis and healthy individuals. Then, changes in circRNA expression in exosomes were studied by circRNA microarray analysis. Gene ontology (GO) analysis and Kyoto City Encyclopaedia of Genes and Genomes (KEGG) pathway analysis were used to annotate the biological functions and pathways of genes, and a circRNA-miRNA-mRNA regulatory network was constructed. In the microarray analysis, 132 circRNAs were significantly differentially expressed, including 80 and 52 that were upregulated and downregulated, respectively. RT-qPCR verified the results of microarray analysis: hsa_circRNA_104484 and hsa_circRNA_104670 were upregulated in sepsis serum exosomes. ROC analysis showed that hsa_circRNA_104484 and hsa_circRNA_104670 in serum exosomes have the potential to be used as diagnostic markers for sepsis. The circRNA-miRNA-mRNA network predicted the potential regulatory pathways of differentially expressed circRNAs. There are differences in the expression of circRNA in serum exosomes between patients with sepsis and healthy individuals, which may be involved in the occurrence and development of the disease. Among them, elevations in hsa_circRNA_104484 and hsa_circRNA_104670 could be used as novel diagnostic biomarkers and molecular therapeutic targets.

Evaluation of a revised resuscitation protocol for out-of-hospital cardiac arrest patients due to COVID-19 safety protocols: a single-center retrospective study in Japan

This study aimed to determine the association between cardiopulmonary resuscitation (CPR) under the coronavirus 2019 (COVID-19) safety protocols in our hospital and the prognosis of out-of-hospital cardiac arrest (OHCA) patients, in an urban area, where the prevalence of COVID-19 infection is relatively low. This was a single-center, retrospective, observational, cohort study conducted at a tertiary critical care center in Kyoto City, Japan. Adult OHCA patients arriving at our hospital under CPR between January 1, 2019, and December 31, 2020 were included. Our hospital implemented a revised resuscitation protocol for OHCA patients on April 1, 2020 to prevent COVID-19 transmission. This study defined the conventional CPR period as January 1, 2019 to March 31, 2020, and the COVID-19 safety protocol period as April 1, 2020 to December 31, 2020. Throughout the prehospital and in-hospital settings, resuscitation protocols about wearing personal protective equipment and airway management were revised in order to minimize the risk of infection; otherwise, the other resuscitation management had not been changed. The primary outcome was hospitalization survival. The secondary outcomes were return of spontaneous circulation after hospital arrival and 1-month survival after OHCA occurrence. The adjusted odds ratios with 95% confidence intervals (CI) were calculated for outcomes to compare the two study periods, and the multivariable logistic model was used to adjust for potential confounders. The study analyzed 443 patients, with a median age of 76 years (65–85), and included 261 men (58.9%). The percentage of hospitalization survivors during the entire research period was 16.9% (75/443 patients), with 18.7% (50/267) during the conventional CPR period and 14.2% (25/176) during the COVID-19 safety protocol period. The adjusted odds ratio for hospitalization survival during the COVID-19 safety protocol period was 0.61 (95% CI 0.32–1.18), as compared with conventional CPR. There were no cases of COVID-19 infection among the staff involved in the resuscitation in our hospital. There was no apparent difference in hospitalization survival between the OHCA patients resuscitated under the conventional CPR protocol compared with the current revised protocol for controlling COVID-19 transmission.

What Caused Dire Clusters ? ~Analysis of Nosocomial COVID-19 Outbreaks in Kyoto City, Japan From the 1st Wave Through 3rd Wave in 2020/21

Fifteen COVID-19 nosocomial clusters occurred in the first year of the pandemic in Kyoto City, involving 232 in-hospital patients and 157 healthcare workers (HCWs). Among them, 40 percent of index cases were HCWs. The in-hospital mortality rate among patients was 18.1%, whereas the mortality rate in the Kyoto City area during the study period was 1.79%. The time needed to contain outbreaks was shorter in hospitals with full-time infection control personnel assigned.