Community assembly and metaphylogeography of soil biodiversity: insights from haplotype-level community DNA metabarcoding within an oceanic island

Most of our understanding of island diversity comes from the study of aboveground systems, while the patterns and processes of diversification and community assembly for belowground biotas remain poorly understood. Here we take advantage of a relatively young and dynamic oceanic island to advance our understanding of eco-evolutionary processes driving community assembly within soil mesofauna. Using whole organism community DNA (wocDNA) metabarcoding and the recently developed metaMATE pipeline, we have generated spatially explicit and reliable haplotype-level DNA sequence data for soil mesofaunal assemblages sampled across the four main habitats within the island of Tenerife. Community ecological and metaphylogeographic analyses have been performed at multiple levels of genetic similarity, from haplotypes to species and supraspecific groupings. Broadly consistent patterns of local-scale species richness across different insular habitats have been found, whereas local insular richness is lower than in continental settings. Our results reveal an important role for niche conservatism as a driver of insular community assembly of soil mesofauna, with only limited evidence for habitat shifts promoting diversification. Furthermore, support is found for a fundamental role of habitat in the assembly of soil mesofauna, where habitat specialism is mainly due to colonisation and the establishment of preadapted species. Hierarchical patterns of distance decay at the community level and metaphylogeographical analyses support a pattern of geographic structuring over limited spatial scales, from the level of haplotypes through to species and lineages, as expected for taxa with strong dispersal limitations. Our results demonstrate the potential for wocDNA metabarcoding to advance our understanding of biodiversity.

Utopian and insular spaces in Chinese literature: An island approach

In response to the relative lack of scholarly attention paid to the relationship between island utopia and Chinese literature, this paper studies the imagination of both island and insular geographies in Chinese ‘utopian’ literature using an island-sensitive approach. Employing an expanded and constructive conception of the island, the paper examines the heterogeneity of Chinese island and insular imaginaries in literary works from diverse historical periods, especially in relation to the dominant western model of the remote tropical oceanic island. Based on the finding that the alterity of Chinese island and insular imagination lies as much in its depiction of spatial ambiguities as in its mixing of diverse figures, I reflect further on the benefits and perils of adopting a west-inflected island approach in examining the imaginary landscapes of utopianism and insularity in Chinese literature. It is argued that Chinese island literature is more a reading effect enabled by an imported theoretical approach than any inherent tradition in itself. In the end, two paths for innovating island aesthetics and epistemologies in cross-cultural contexts are proposed.

Population-specific patterns of toxin sequestration in monarch butterflies from around the world

Monarch butterflies are one of the preeminent examples of a toxin-sequestering animal, gaining protection against predators via cardenolides obtained from their milkweed host plants. Although cardenolide sequestration by monarchs has been studied in ecological, physiological, and phylogenetic contexts, relatively little research has surveyed genetic variation in the ability to sequester, nor has monarch sequestration been studied in relation to divergent host plant assemblages or variation in exposure to predation. Here, we use the monarch's recent global range expansion to test hypotheses about how cardenolide sequestration evolves over relatively contemporary time scales. First, we test for whether sympatric monarch/milkweed combinations have a sequestration advantage by rearing six geographically disparate monarch populations on six associated milkweed host species and measuring levels of sequestered cardenolides in a set of 440 adult butterflies. Second, we use monarchs from Guam - an oceanic island where birds have been functionally extirpated for approximately 40 years - to test hypotheses about how exposure to avian predation affects cardenolide sequestration. We find little overall evidence for increased sequestration on sympatric hosts. However, one monarch population (Puerto Rico) shows strong support for cross-host tradeoffs in sequestration ability, primarily driven by limited sequestration of polar cardenolides from two temperate North American milkweeds (Asclepias syriaca and A. speciosa). Monarchs from Guam show some evidence for reduced cardenolide sequestration in both a cross-island comparison of wild-caught butterflies as well as population-level comparisons of greenhouse-reared butterflies. Our results suggest that there is substantial genetic variation in sequestration ability (both within and between monarch populations) and that evolutionary history and contemporary species interactions may influence patterns of cardenolide sequestration.

Strong horizontal and vertical connectivity in the coral Pocillopora verrucosa from Ludao, Taiwan, a small oceanic island

Mesophotic habitats could be sheltered from natural and anthropogenic disturbances and act as reproductive refuges, providing propagules to replenish shallower populations. Molecular markers can be used as proxies evaluating the connectivity and inferring population structure and larval dispersal. This study characterizes population structure as well as horizontal and vertical genetic connectivity of the broadcasting coral Pocillopora verrucosa from Ludao, a small oceanic island off the eastern coast of Taiwan. We genotyped 75 P. verrucosa specimens from three sites (Gongguan, Dabaisha, and Guiwan) at three depth ranges (Shallow: 7–15 m, Mid-depth: 23–30 m, and Deep: 38–45 m), spanning shallow to upper mesophotic coral reefs, with eight microsatellite markers. F-statistics showed a moderate differentiation (FST = 0.106, p<0.05) between two adjacent locations (Dabaisha 23–30 and Dabaisha 38–45 m), but no differentiation elsewhere, suggesting high levels of connectivity among sites and depths. STRUCTURE analysis showed no genetic clustering among sites or depths, indicating that all Pocillopora individuals could be drawn from a single panmictic population. Simulations of recent migration assigned 30 individuals (40%) to a different location from where they were collected. Among them, 1/3 were assigned to deeper locations, 1/3 to shallower populations and 1/3 were assigned to the right depth but a different site. These results suggest high levels of vertical and horizontal connectivity, which could enhance the recovery of P. verrucosa following disturbances around Ludao, a feature that agrees with demographic studies portraying this species as an opportunistic scleractinian.

Tourism and urban development as drivers for invertebrate diversity loss on tropical islands

Oceanic islands harbour a disproportionately high number of endemic and threatened species. Rapidly growing human populations and tourism are posing an increasing threat to island biota, yet the ecological consequences of these human land uses on small oceanic island systems have not been quantified. Here, we investigated and compared the impact of tourism and urban island development on ground-associated invertebrate biodiversity and habitat composition on oceanic islands. To disentangle tourism and urban land uses, we investigated Indo-Pacific atoll islands, which either exhibit only tourism or urban development, or remain uninhabited. Within the investigated system, we show that species richness, abundance and Shannon diversity of the investigated invertebrate community are significantly decreased under tourism and urban land use, relative to uninhabited islands. Remote-sensing-based spatial data suggest that habitat fragmentation and a reduction in vegetation density are having significant effects on biodiversity on urban islands, whereas land use/cover changes could not be linked to the documented biodiversity loss on tourist islands. This offers the first direct evidence for a major terrestrial invertebrate loss on remote oceanic atoll islands due to different human land uses with yet unforeseeable long-term consequences for the stability and resilience of oceanic island ecosystems.

Petrotectonic origin of mafic eclogites from the Maksyutov subduction complex, south Ural Mountains, Russia

ABSTRACT The Maksyutov complex is a mid- to late-Paleozoic high- to ultrahigh-pressure (HP-UHP) eclogite-bearing subduction zone terrane in the south Ural Mountains. Previous reports of radial fractures emanating from quartz inclusions in garnet, omphacite, and glaucophane, cuboid graphite pseudomorphs after matrix diamond, and microdiamond aggregates preserved in garnet identified by Raman spectroscopy indicate that parts of the complex were subjected to physical conditions of ∼600 °C and &gt;2.8 GPa for coesite-bearing rocks, and &gt;3.2 GPa for diamond-bearing rocks. Peak UHP eclogite-facies metamorphism took place at ca. 385 Ma, and rocks were exhumed through retrograde blueschist-facies conditions by ca. 360 Ma. Bulk analyses of 18 rocks reflect the presence of mid-oceanic-ridge basalt (MORB), oceanic-island basalt (OIB), and island-arc tholeiite (IAT) basaltic and andesitic series plus their metasomatized equivalents. To more fully constrain the petrotectonic evolution of the complex, we computed isochemical phase equilibria models for representative metabasites in the system Na2O-CaO-K2O-FeO-MgO-Al2O3-SiO2-H2O-TiO2 based on our new bulk-rock X-ray fluorescence (XRF) data. Both conventional Fe-Mg exchange thermometry and phase equilibrium modeling result in higher peak equilibrium temperatures than were previously reported for the complex. Pseudosection analysis provides minimum P-T conditions of 650–675 °C and 2.4–2.6 GPa for peak assemblages of the least retrogressed Maksyutov eclogites, whereas Fe-Mg exchange thermometry yields temperatures of 750 ± 25 °C for a pressure of 2.5 GPa. We interpret our new P-T data to reflect a thermal maximum reached by the eclogites on their initial decompression-exhumation stage, that defines a metamorphic field gradient; the relict coesite and microdiamond aggregates previously reported testify to pressure maxima that define an earlier prograde subduction zone gradient. The eclogitic Maksyutov complex marks underflow of the paleo-Asian oceanic plate and does not represent subduction of the Siberian cratonal margin.