The Potential Effect of Silver Nanoparticles on the Expression and Levels of Skin Matrix Metalloproteinases (Gelatinases): In Vivo Assessment

The current work aimed to investigate the enzymatic activities and the expression profiles of the metalloproteinases MMP-2 and MMP-9 in the skin of rabbits as an animal model, upon sub-acute dermal exposure to Ag-NPs. This was done in order to determine their potential effect on these proteolytic enzymes which have been traditionally involved in many physiological processes such as inflammation and wound healing which are among the main applications Ag-NPs containing medical products. The rabbits were topically exposed to every other day dose of 0.5% Ag-NPs for 7- and 14-days experimental periods. Both periods were followed by 7 days recovery time. The results revealed the remarkable effect of Ag-NPs on MMPs at both the enzyme production and the transcriptional levels. The effect was time dependent where no significant changes were found after the 7 days of exposure, while the concentrations of MMP-2 and MMP-9 as well as the expression of their encoding genes were significantly elevated in the skin of the exposure and recovery groups at the 14 days experimental period. MMP-9 was the most affected enzyme, whereas MMP-2 showed the minimal effect. Several mechanisms and pathways have been suggested to explain the disturbance of MMPs which require further molecular studies to obtain more evidence supporting the most probable explanation.

Exploring the Association between Alcohol Drinking and Physical Activity in Adolescence; Two-Year Prospective Study in Younger Adolescents from Bosnia and Herzegovina

Insufficient physical activity and alcohol consumption (AC) are important health-threatening behaviors in adolescence, but there are controversial findings regarding the association that may exist between AC and physical activity levels (PALs) at this age. This study aimed to prospectively examine the relationship that may exist between AC and PAL in younger adolescence, considering the potential confounding effect of sports participation. The participants (n = 669, 337 females) were adolescents from Bosnia and Herzegovina who were tested on two occasions, at baseline (14 years of age) and again at follow-up (16 years of age). The variables included AC (as indicated by the AUDIT questionnaire), sports participation, age, gender (predictors), and PAL (obtained by the PAQ-A questionnaire) criterion. The results indicated that PAL at baseline was higher in those adolescents (boys) with a higher AUDIT score, but this association was partially confounded by sports participation. Multinomial regression indicated a higher risk of a decline in PAL over the study course in adolescents with a higher AUDIT score at baseline (OR = 1.32, 95%CI: 1.11–1.54 for being in the high-risk group for a decline in PAL). The most probable explanation is likely found in the high drop-out from sports in the studied period and earlier initiation of AC in adolescents involved in sports. Public health and sports authorities should urgently act preventively and develop educational programs against alcohol drinking in youth athletes.

P04.23 Multinodular and vacuolating neuronal tumor - advanced MRI features

BACKGROUND A rare pathology, multinodular and vacuolating neuronal tumors (MVNT) have been first described in 2013 and included in the 2016 World Health Organization Classification of Tumors of the Central Nervous System as having a distinctive radiological and cytoarchitectural appearance. This study aims to evaluate the imaging features of MVNT, it’s differential diagnosis, and possible involvement in adult-onset epilepsy. MATERIAL AND METHODS We present the case of a 29-year-old male, smoker, and drug abuser, who developed a seizure after 25 hours’ sleep deprivation, alcohol, energy drinks, cigarettes, and cocaine consumption. Cerebral CT, MRI, electroencephalogram, MR spectroscopy, and tractography was performed. RESULTS The MRI performed revealed a right, cortico-subcortical pseudo-nodular temporal lesion, non-enhancing, with a slight thickening of the adjacent cortex and right hippocampal involvement. The neurological examination was normal, but the electroencephalogram showed a right temporal irritative pattern. MR- Spectroscopy described a slight increase of choline (Cho), reduction of N-acetylaspartate (NAA), and also increased myo-inositol peak, ruling out a high-grade tumoral substrate. A tractography was also performed, excluding the other alternative diagnosis of focal cortical dysplasia, thus leaving MVNT to be the most probable explanation for the lesion. CONCLUSION Advanced MRI features were useful to increase the diagnosis accuracy. The future management consisted of image surveillance, anticonvulsant treatment, and lifestyle improvement. Adult-onset seizures are among the most frequent discovery possibilities for MVNT. In our case, it is to be seen whether the lifestyle changes and medication will prevent future seizures.

Treewidth-Aware Cycle Breaking for Algebraic Answer Set Counting

Probabilistic reasoning, parameter learning, and most probable explanation inference for answer set programming have recently received growing attention. They are only some of the problems that can be formulated as Algebraic Answer Set Counting (AASC) problems. The latter are however hard to solve, and efficient evaluation techniques are needed. Inspired by Vlasser et al.'s Tp-compilation (JAR, 2016), we introduce Tp-unfolding, which employs forward reasoning to break the cycles in the positive dependency graph of a program by unfolding them. Tp-unfolding is defined for any normal answer set program and unfolds programs with respect to unfolding sequences, which are akin to elimination orders in SAT-solving. Using "good" unfolding sequences, we can ensure that the increase of the treewidth of the unfolded program is small. Treewidth is a measure adhering to a program's tree-likeness, which gives performance guarantees for AASC. We give sufficient conditions for the existence of good unfolding sequences based on the novel notion of component-boosted backdoor size, which measures the cyclicity of the positive dependencies in a program. The experimental evaluation of a prototype implementation, the AASC solver aspmc, shows promising results.

SECONDARY HEMOPHAGOCYTIC LYMPHOHISTIOCYTOSIS IN A CHILD WITH CONCOMITANT EBSTEIN BARR VIRUS AND SCRUB TYPHUS INFECTION – A RARE ENTITY

Scrub typhus is a life threatening zoonosis caused by Orientia tsutsugamushi organisms that are transmitted by the larvae of trombiculid mites. It is endemic to a geographically distinct region, the so called Tsutsugamushi triangle, which includes Japan, China and South Korea. The disease is more prevalent in southern and northern India.It is characterised by focal or disseminated vasculitis and perivasculitis involving the lungs, liver, spleen and central nervous system. We report our experience with pediatric scrub typhus at a hospital in eastern India with EBV positive and secondary HLH. An 8 year old boy with fever, maculopapular rash, hepatosplenomegaly and lymphadenopathy was admitted in our institution and diagnosed with Scrub typhus. Physical and laboratory data showed hepatosplenomegaly, bicytopenia, hyperferritinemia, and hypobrinogenemia. Secondary HLH was diagnosed and the child was managed with IVIG and steroids. In view of rash followed by lymphadenopathy and hepatosplenomegaly EBV serology was also sent as a cause of HLH. Surprisingly, EBV panel was also positive. We therefore concluded that the most probable explanation was EBV triggered HPS following scrub typhus infection. Another possible explanation is EBV can be reactivated in critically ill patients. To our knowledge this is the rst such case in the pediatric population reported till date.

Orientation of buried people in the necropolises of Bystryanka culture (northern foothills of Altai, Scythian-Saka time)

The article presents the results of studying the orientation of buried in a certain sector of the horizon on the basis of materials from the excavations of the necropolises of the Bystryanka archaeological culture. Information about 168 burials investigated at 26 necropolises of the northern foothills of Altai of the Scythian-Saka period was used. It has been established that the dominant tradition was to direct the buried people with their heads to the western sector of the horizon. At the same time, deviations are recorded due to various factors. A number of approaches traditionally used in this kind of research were used to interpret the identified orientations. The authors concluded that the observed deviations from the westerly direction are associated with the seasonal movement of the sun. At the same time, a possible basis on which the population of Bystryanka culture relied when orienting the dead is the sunset point. The most probable explanation for the spread of directions other than those in the West is the contacts with carriers of other cultural traditions. Opportunities for further study and interpretation of the revealed patterns in the orientation of the buried people based on the materials of the Bystryanka culture are associated with a more detailed analysis of individual necropolises, including the specification of the landscape features of specific monuments. Of particular importance is the expansion of the data used by attracting information about unpublished complexes. In addition, the correlation of the considered element of the ritual with other indicators of funeral practice, including the peculiarities of the position of the buried people and the traditions of placing goods in the grave, seems promising.

MPE Computation in Bayesian Networks Using Mini-Bucket and Probability Trees Approximation

Given a set of uncertain discrete variables with a joint probability distribution and a set of observations for some of them, the most probable explanation is a set or configuration of values for non-observed variables maximizing the conditional probability of these variables given the observations. This is a hard problem which can be solved by a deletion algorithm with max marginalization, having a complexity similar to the one of computing conditional probabilities. When this approach is unfeasible, an alternative is to carry out an approximate deletion algorithm, which can be used to guide the search of the most probable explanation, by using A* or branch and bound (the approximate+search approach). The most common approximation procedure has been the mini-bucket approach. In this paper it is shown that the use of probability trees as representation of potentials with a pruning of branches with similar values can improve the performance of this procedure. This is corroborated with an experimental study in which computation times are compared using randomly generated and benchmark Bayesian networks from UAI competitions.

MAP Inference for Probabilistic Logic Programming

In Probabilistic Logic Programming (PLP) the most commonly studied inference task is to compute the marginal probability of a query given a program. In this paper, we consider two other important tasks in the PLP setting: the Maximum-A-Posteriori (MAP) inference task, which determines the most likely values for a subset of the random variables given evidence on other variables, and the Most Probable Explanation (MPE) task, the instance of MAP where the query variables are the complement of the evidence variables. We present a novel algorithm, included in the PITA reasoner, which tackles these tasks by representing each problem as a Binary Decision Diagram and applying a dynamic programming procedure on it. We compare our algorithm with the version of ProbLog that admits annotated disjunctions and can perform MAP and MPE inference. Experiments on several synthetic datasets show that PITA outperforms ProbLog in many cases.

Probable causes and risk factors for positive SARS-CoV-2 test in recovered patients: Evidence from Brunei Darussalam

We report findings of a national study in Brunei Darussalam indicating that one in five recovered patients subsequently test positive again for SARS-CoV-2 - this risk is nearly three times higher in older patients (age 53 and above) than younger ones (below age 53). Review of clinical and epidemiological records do not support reinfection or reactivation as likely causes of the 're-positive' observation. Instead, prolonged but intermittent viral shedding is the most probable explanation. We discuss the implications of these findings for infection control and clinical practice.

Optical validation and characterization of Planck PSZ2 sources at the Canary Islands observatories

Aims. The second catalogue of Planck Sunyaev–Zeldovich (SZ) sources, hereafter PSZ2, is the largest sample of galaxy clusters selected through their SZ signature in the full sky. At the time of publication, 21% of these objects had no known counterpart at other wavelengths. Using telescopes at the Canary Island observatories, we conducted the long-term observational programme 128-MULTIPLE-16/15B (hereafter LP15), a large and complete optical follow-up campaign of all the unidentified PSZ2 sources in the northern sky, with declinations above −15° and no correspondence in the first Planck catalogue PSZ1. The main aim of LP15 is to validate all those SZ cluster candidates, and to contribute to the characterization of the actual purity and completeness of full Planck SZ sample. In this paper, we describe the full programme and present the results of the first year of observations. Methods. The LP15 programme was awarded 44 observing nights, spread over two years in three telescopes at the Roque de los Muchachos Observatory. The full LP15 sample comprises 190 previously unidentified PSZ2 sources. For each target, we performed deep optical imaging and spectroscopy. Our validation procedure combined this optical information with SZ emission as traced by the publicly available Planck Compton y-maps. The final classification of the new galaxy clusters as optical counterparts of the SZ signal is established according to several quantitative criteria based on the redshift, velocity dispersion, and richness of the clusters. Results. This paper presents the detailed study of 106 objects out of the LP15 sample, corresponding to all the observations carried out during the first year of the programme. We confirmed the optical counterpart for 41 new PSZ2 sources, 31 of them being validated using also velocity dispersion based on our spectroscopic information. This is the largest dataset of newly confirmed PSZ2 sources without any previous optical information. All the confirmed counterparts are rich structures (i.e. they show high velocity dispersion), and are well aligned with the nominal Planck coordinates (i.e. ∼70% of them are located at less than 3′ distance). In total, 65 SZ sources are classified as unconfirmed, 57 of them being due to the absence of an optical over-density, and eight of them due to a weak association with the observed SZ decrement. Most of the sources with no optical counterpart are located at low galactic latitudes and present strong galactic cirrus in the optical images, the dust contamination being the most probable explanation for their inclusion in the PSZ2 catalogue.