The breeding of timothy (Phleum pratense L.) is mainly targetted at improving the nutritive value of the crop. Three physical properties, packed volume (PV), water retention (WR) and water solubility (WS) have been suggested as indirect measurements of voluntary intake. Variation for these three traits was estimated at the early heading and the flowering stages for 2 consecutive years within four timothy populations (Itasca, Labelle, Pronto and Richmond). Significant genetic variation was observed for PV, WR, WS, plant height (PH), dry matter yield (DMY), crude protein (CP), and digestibility (IVDMD) but the magnitude of the variation was inconsistent among populations. Curiously, PV, WR and WS seemed to improve from early heading to flowering. Significant variation was observed in all four populations for PV, in three populations for WS and in only two populations for WR. The different interactions between genotypes, years and maturities indicated that different selection strategies might be needed according to the base population used. The correlations within and between maturity stages among PV, WR, WS, PH, DMY, CP and IVDMD were inconsistent among populations. The presence of genetic variation in timothy for the three physical properties under study suggests that improvement of these traits may be possible through breeding. However, further information is needed on the heritability and the evolution of these traits with advancing maturity.Key words: Timothy, Phleum pratense L., packed volume, water retention, water solubiliy, forage quality