Nickel-Catalyzed N-Arylation of Amides with (Hetero)aryl Electrophiles using a DBU/NaTFA Dual-Base System

The first nickel-catalyzed N-arylation of amides with (hetero)aryl (pseudo)halides employing an organic amine base is described. When using Ni(COD)2/CyPAd-DalPhos catalyst mixtures in combination with DBU/NaTFA as a dual-base system, a diversity of (hetero)aryl chloride, bromide, tosylate, and mesylate electrophiles were successfully cross-coupled with structurally varied primary amides, as well as a selection of secondary amide, lactam, and oxazolidone nucleophiles.

Vertical organic permeable dual-base transistors for logic circuits

The main advantage of organic transistors with dual gates/bases is that the threshold voltages can be set as a function of the applied second gate/base bias, which is crucial for the application in logic gates and integrated circuits. However, incorporating a dual gate/base structure into an ultra-short channel vertical architecture represents a substantial challenge. Here, we realize a device concept of vertical organic permeable dual-base transistors, where the dual base electrodes can be used to tune the threshold voltages and change the on-currents. The detailed operation mechanisms are investigated by calibrated TCAD simulations. Finally, power-efficient logic circuits, e.g. inverter, NAND/AND computation functions are demonstrated with one single device operating at supply voltages of <2.0 V. We believe that this work offers a compact and technologically simple hardware platform with excellent application potential for vertical-channel organic transistors in complex logic circuits.

Organic Complementary Inverters Based on Vertical-Channel Dual-Base Thin-Film Transistors with Time Constants < 10ns

Lateral-channel dual-gate organic thin-film transistors (OTFTs) are utilized in organic pseudo-CMOS inverters to realize switching voltage control. However, the relatively long channel length will slow the inverter operation. Vertical-channel dual-gate OTFTs are an attractive alternative due to the short channel length. In this work, controllable and reliable complementary inverters are presented using vertical n-channel organic permeable dual-base transistors (OPDBTs) and vertical p-channel organic permeable base transistors (OPBTs). With operating voltages < 2.0 V, the threshold voltages of the n-type OPDBTs are changed across a wide range from 0.12 to 0.82 V by varying the voltage of the additional base. The fabricated tunable organic complementary inverter features switching voltage shift and gain enhancement. In addition, the inverters show very small switching time constants (< 10 ns) at 10 MHz. Our work represents a significant step towards the application of vertical dual-gate/base transistors in power-efficient organic complementary inverters, offering the capability to easily tune the switching voltage of organic complementary inverters. This facilitates the development of high-performance and complex organic digital integrated circuits.

A virtual sequencer reveals the dephasing patterns in error-correction code DNA sequencing

An error-correction code (ECC) sequencing approach has recently been reported to effectively reduce sequencing errors by interrogating a DNA fragment with three orthogonal degenerate sequencing-by-synthesis (SBS) reactions. However, similar to other non-single-molecule SBS methods, the reaction will gradually lose its synchronization within a molecular colony in ECC sequencing. This phenomenon, called dephasing, causes sequencing error, and in ECC sequencing, induces distinctive dephasing patterns. To understand the characteristic dephasing patterns of the dual-base flowgram in ECC sequencing and to generate a correction algorithm, we built a virtual sequencer in silico. Starting from first principles and based on sequencing chemical reactions, we simulated ECC sequencing results, identified the key factors of dephasing in ECC sequencing chemistry, and designed an effective dephasing algorithm. The results show that our dephasing algorithm is applicable to sequencing signals with at least 500 cycles, or 1,000-bp average read length, with acceptably low error rate for further parity-checks and ECC deduction. Our virtual sequencer with our dephasing algorithm can further be extended to a dichromatic form of ECC sequencing, allowing for a potentially much more accurate sequencing approach.

Rapid Penetration Path Planning Method for Stealth UAV in Complex Environment with BB Threats

This paper presents the flight penetration path planning algorithm in a complex environment with Bogie or Bandit (BB) threats for stealth unmanned aerial vehicle (UAV). The emergence of rigorous air defense radar net necessitates efficient flight path planning and replanning for stealth UAV concerning survivability and penetration ability. We propose the improved A-Star algorithm based on the multiple step search approach to deal with this uprising problem. The objective is to achieve rapid penetration path planning for stealth UAV in a complex environment. Firstly, the combination of single-base radar, dual-base radar, and BB threats is adopted to different threat scenarios which are closer to the real combat environment. Besides, the multistep search strategy, the prediction technique, and path planning algorithm are developed for stealth UAV to deal with BB threats and achieve the penetration path replanning in complex scenarios. Moreover, the attitude angle information is integrated into the flight path which can meet real flight requirements for stealth UAV. The theoretical analysis and numerical results prove the validity of our method.

IMPLEMENTASI KURIKULUM BERBASIS INDUSTRI DI SMK MITRA INDUSTRI MM2100 CIKARANG - BEKASI

INDUSTRIAL BASED CURRICULUM IMPLEMETATION AT SMK MITRA INDUSTRI MM2100 CIKARANG-BEKASIThis study aims to obtain a fact-based picture in the form of data and information regarding the implementation of an industry-based curriculum at SMK Mitra Industri MM2100. This study uses a qualitative method, which is to make a complex and comprehensive picture with detailed descriptions from the eyes of the informants to produce a comprehensive exposure based on clear and real findings, events, processes and results related to the implementation of an industry-based curriculum. Data obtained through interviews, field observations and documentation studies. Based on data analysis, the following results are obtained: 1). The curriculum development model used at SMK Mitra Industri MM2100, is a model of curriculum development that is problem-centered by integrating several disciplines to form a concept of attitudes, skills and knowledge in accordance with the demands and needs of the industry which are oriented to the vision, mission and goals of the school. 2). The learning process at SMK Mitra Industri MM2100 uses a block system, which is designed to be oriented towards the merging of instruction and construction, so that the learning process approach refers to the dual base program to fully and fully cultivate industrial work patterns. 3). Challenges and obstacles in the implementation of industrial-based curriculum in SMK Mitra Industri MM2100 in the form of not wanting to change the principles of school/mental blockage, focusing on knowledge, school target is focus on graduation of students, not on the distribution of work at industries and bad networking with industry which can be overcome through socialization to all elements of the school and stakeholders involved.

Both A-to-G and C-to-T base editing in human cells with dual base editor (A&C-BEmax)

As promising genome editing tools, cytidine (CBEs) and adenosine base editors (ABEs) efficiently catalyze C•G to T•A and A•T to G•C conversions, respectively. However, CBEs or ABEs only generate single type of nucleotide conversions, which limits the sequence diversity of edited site and therapeutic applications at certain circumstances. A dual base editor(A&C-BEmax) has been developed to achieve both C•G to T•A and A•T to G•C mutations through fusion of cytidine and adenosine deaminases to Cas9n. In this protocol, we demonstrate the generation of diverse point mutations, including a simultaneous -113A-to-G and -114C-to-T conversions, in the hemoglobin gamma (HBG) gene promoter region in HUDEP-2 cells through A&C-BEmax with a single sgRNA, leading to reactivation of γ-globin for the potential treatment of β-hemoglobinopathy. A&C-BEmax is a valuable tool not only for dissecting the function of genomic sequence at a single base resolution but also for the therapeutics of genetic disorders. This step-by-step protocol is related to the publication “Dual base editor catalyzes both cytidine and adenine base conversions in human cells” in Nature Biotechnology.