Knockdown of NON-YELLOW COLORING 1 (NYC1)-like gene or chlorophyllin application enhanced chlorophyll accumulation with antioxidant roles in suppressing heat-induced leaf senescence in perennial ryegrass

Loss of chlorophyll (Chl) and oxidative damages co-occur during heat-induced leaf senescence. This study aimed to determine the functions of Chl catabolic gene, NON-YELLOW COLORING 1 (NYC1)-like (NOL) in regulating heat-induced leaf senescence and to characterize antioxidant roles of a Chl derivative, sodium copper chlorophyllin (SCC), in suppressing heat-induced leaf senescence. In two separate experiments, one by comparing NOL RNAi transgenic and wild-type plants, and the other by analyzing the effects of sodium copper chlorophyllin (SCC, 1 mM) treatment, perennial ryegrass (Lolium perenne) were exposed to heat stress (38/35 oC, day/night) or optimal temperature (25/20 oC). Results showed that both knockdown of LpNOL and application of SCC suppressed heat-induced leaf senescence, as manifested by increased Chl content, reduced electrolyte leakage, and down-regulation of Chl-catabolic genes and senescence-related genes, as well as enhanced antioxidant capacity in the peroxidase (POD) pathway for H2O2 scavenging. Ex vivo SCC incubation protected membranes from H2O2 damage onto mesophyll protoplasts of perennial ryegrass. The suppression of leaf senescence by knockdown of NOL or chlorophyllin application was associated with enhanced chlorophyll accumulation playing antioxidant roles in protecting leaves from heat-induced oxidative damages.

Enhanced photothermal signal detection by graphene oxide integrated long period fiber grating for on-site quantification of sodium copper chlorophyllin

An enhanced photothermal signal detection method based on graphene oxide (GO) integrated long period fiber grating (LPFG) for on-site quantification of sodium copper chlorophyllin (SCC) concentration is proposed. SCC, as...

In Situ Synthesis of a Double-Layer Chitosan Coating on Cotton Fabric to Improve the Color Fastness of Sodium Copper Chlorophyllin

Natural dye’s poor affinity for cotton and poor fastness properties still hinder its applications in the textile industry. In this study, a doubled-layered chitosan coating was cured on cotton fabric to serve as bio-mordant and form a protective layer on it. Under the optimal treatment conditions, the maximum qe (adsorption amount) of the natural dye sodium copper chlorophyllin (SCC) calculated from the Langmuir isothermal model was raised from 4.5 g/kg to 19.8 g/kg. The dye uptake of the treated fabric was improved from 22.7% to 96.4% at 1% o.w.f. dye concentration. By a second chitosan layer cured on the dyed fabric via the cross-linking method, the wash fastness of the cotton fabric dyed with SCC can be improved from 3 to 5 (ISO 105 C-06). The natural source of the biopolymer material, chitosan, and its ability to biodegrade at end of life met with the initial objective of green manufacturing in applying natural dyes and natural materials to the textile industry.