Abstract: The article presents the results of a psychophysiological examination of drivers of passenger and export traffic, working in the day and night shifts, respectively. The dynamics of performing psychophysiological tests (changes in the reaction rate, the number of errors, perception of time intervals, etc.) after day and night shifts was revealed, and differences in the subjective perception of the specifics of work in the daytime and at night were also noted. Working the night shift requires the driver to mobilize psychophysiological resources aimed at maintaining active wakefulness and fighting monotony. Day trips are perceived to be more stressful due to more input and traffic. Target: The study of the functional state dynamics of train drivers working without an assistant during day and night trips with an increase in the duration of working hours up to 8 hours in passenger traffic and up to 12 - in export traffic. Methods: 1. Express test of the functional state; 2. «Sense of time» test; 3. Stress resistance test; 4. Survey «Well-being. Activity, mood (SAN)»; 5. Survey «Diagnostics of states of reduced performance (DORS)». Results: The dynamics of the speed and stability of the visual-motor reaction, the accuracy of the perception of time intervals, as well as the subjective perception of the features of day and night shifts by train drivers themselves, makes it possible to distinguish differences in the specifics of shift work: in the daytime it is distinguished by greater intensity, tension, which is reflected in the number of erroneous actions during testing after a day's ride on the simulator, and in the subjective experience of stress noted by the drivers. The need to work at night requires considerable efforts from train drivers to mobilize, which is manifested during a psychophysiological examination before the night shift, however, forced wakefulness during night work leads to a state of monotony among train drivers.