AbstractNeoichnological research of terrestrial tracemakers in coastal settings provides important palaeoenvironmental information about their context within the subaerial facies. Here we present the first geophysical dataset of reptile burrows in a carbonate substrate and use it to help visualize parts of the burrows of the Bahamian (San Salvador) rock iguana (Cyclura rileyi). High-resolution 800 MHz ground-penetrating radar (GPR) images within an enclosure on San Salvador Island were employed to discriminate between the electromagnetic signal response from subsurface anomalies related to air-dominated voids or live animals within burrows. The dielectric contrast between the carbonate substrate and open burrows was sufficient to identify the majority of 15-20-cm-wide subsurface extensions of the inclined tunnels in the upper 30-40 cm. Whereas limestone clasts induced some interference, it is possible to differentiate their high-amplitude diffractions from those produced by the iguana burrows. Our research indicates that GPR imaging is a viable, rapid, non-invasive method of visualizing animal burrows, with implications to neoichnology, paleoichnology, and conservation ecology of semi-fossorial species. Furthermore, the critically endangered status of Bahamian land iguanas, as well as ongoing threats from natural and introduced pressures, highlights the need for research into their ichnological record.