Hand and face somatotopy shown using MRI-safe vibrotactile stimulation with a novel Soft Pneumatic Actuator (SPA)-Skin interface

The exact somatotopy of the human facial representation in the primary somatosensory cortex (S1) remains debated. One reason that progress has been hampered is due the methodological challenge of how to apply automated vibrotactile stimuli to face areas in a manner that is: 1) reliable despite different curvature depending on the face location; and 2) MR-compatible and free of MR-interference artefacts when applied in the MR head-coil. Here we overcame this challenge by using soft pneumatic actuator (SPA) technology. SPAs are made of a soft silicon material and can be in- or deflated by means of airflow, have a small diameter, and are flexible in structure, enabling good skin contact even on curved body surfaces (as on the face). Here, we aimed to provide a methodological advance by providing automated tactile vibration stimulation inside the head-coil of the MRI. As a sanity check, we first mapped the well-characterised S1 finger layout using this novel device. We found that tactile stimulation of the fingers elicited characteristic somatotopic finger activations in S1, validating the use of our SPA-setup to map somatotopic representations. Ultimately, we used the device to automatically and systematically deliver somatosensory stimulation to different face locations. We found that the forehead representation was least distance from the representation of the hand. Within the face representation, we found that the lip representation is most distant from the forehead representation, with the chin represented in between. Together our results show that, by providing vibrotactile stimulation using the SPA-technology, we are able to reveal clear somatotopic representational patterns.

The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood

Recent advances suggest that miniaturised mid-infrared (MIR) devices could replace more time-consuming, laboratory-based techniques for clinical diagnostics. This work uses Fourier transform infrared spectroscopy to show that the MIR complex refractive index of whole blood varies across a range of haematocrit. This indicates that the use of an evanescent measurement is not sufficient to optically exclude the cellular content of blood in the MIR, as previously assumed. Here, spectral refractive index data is presented in two ways. First, it is given as whole blood with varying haematocrit. Second, it is given as the percentage error that haematocrit introduces to plasma. The maximum error in the effective plasma refractive index due to the haematocrit of healthy adults was 0.25% for the real part n and 11% for the imaginary part k. This implies that calibration measurements of haematocrit can be used to account for errors introduced by the cellular content, enabling plasma spectra and analyte concentrations to be indirectly calculated from a whole blood sample. This methodological advance is of clinical importance as plasma concentration of analytes such as drugs can be determined using MIR without the preprocessing of whole blood.

The Institutional Grammar: A Method for Coding Institutions and its Potential for Advancing Third Sector Research

Institutions—defined as strategies, norms and rules (Ostrom Understanding institutional diversity, Princeton University Press, Princeton, 2005)—are omnipresent in third sector contexts. In this paper, we present the Institutional Grammar (IG) as a theoretically informed approach to support institutional analysis in third sector research. More specifically, the IG coding syntax allows the researcher to systematically wade through rich text and (transcribed) spoken language to identify and dissect institutional statements into finer syntactical segments of interest to the researcher. It is a versatile method that can generate data for small- or large-N research projects and can be integrated with mixed-method research designs. After first introducing and describing the IG, we present a case study to illustrate how a IG-based syntactic analysis can be leveraged to inform third sector research. In the case, we ask: Do the rules embedded in regulatory text addressing the involuntary dissolution of charity organizations differ between bifurcated and unitary jurisdictions in the United States? Using IG’s ABDICO 2.0 syntax, we identify eleven “Activation Condition” (AC) categories that trigger action and assess variation among the 46 jurisdictions. We ultimately conclude that the rules do not differ between bifurcated and unitary jurisdictions, but that finding is not the primary concern. The case demonstrates IG as an important methodological advance that yields granular, structured analyses of rules, norms and strategies in third sector settings that may be difficult to identify with other methods. We then emphasize four areas of third sector research that could benefit from the addition of IG-based methods: analysis of (1) rule compliance, (2) inter-organizational collaboration, (3) comparative study of institutional design, and (4) the study of institutional change. We close the paper with some reflections on where IG-based analysis is headed.

Elementary Students’ Reasoning in Drawn Explanations Based on a Scientific Theory

Constructing explanations of scientific phenomena is a high-leverage practice that promotes student understanding. In the context of this study, we acknowledge that children are used to receiving explanations from teachers. However, they are rarely encouraged to construct explanations about the causes and consequences of phenomena. We modified a strategy to elicit and analyze primary students’ reasoning based on scientific theory as a methodological advance in learning and cognition. The participants were fourth-graders of middle socioeconomic status in Chile’s geographical zone with high seismic risk. They drew explanations about the causes and consequences of earthquakes during a learning unit of eighteen hours oriented toward explanation-construction based on the Tectonic Plates Theory. A constant comparative method was applied to analyze drawings and characterize students’ reasoning used in pictorial representations, following the first coding step of the qualitative Grounded Theory approach. The results show the students expressed progressive levels of reasoning. However, several participants expressed explanations based on the phenomena causes even at an early stage of formal learning. More sophisticated reasoning regarding the scientific theory underpinning earthquakes was found at the end of the learning unit. We discuss approaching elementary students’ scientific reasoning in explanations based on theory, connected with context-based science education.

Performance of bidimensional location quotients for constructing input–output tables

This article seeks to verify the extent to which the formulation of two-dimensional location quotients (2D-LQ) entails a methodological advance in building or generating economic accounts related to sub-territories drawing from basic information. The input–output tables of the Euro Area 19 for 2010 and 2015 are references for analysis. We have used five statistics to measure similarity between true domestic coefficient matrices for ten countries (Austria, Belgium, Estonia, France, Germany, Italy, Latvia, Slovakia, Slovenia, and Spain) and the matrices they generate using nonsurvey techniques (CILQ, FLQ, AFLQ, and 2D-LQ). The focus substantially centers on ranking methodological efficiency by comparing the results of the four techniques mentioned above. The scope of the work employs standard parameters (associated with 2D-LQ) as guidance to ascertain the optimum parameters.

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.

Natural Carbon Sinks Linked to Pastoral Activity in S Spain: A Territorial Evaluation Methodology for Mediterranean Goat Grazing Systems

Exploring and developing new tools for the accounting and management of natural C sinks will provide a closer, more accurate option to remark the importance of such sinks in relation to livestock production, helping to support the persistence of some seriously endangered traditional, environmentally sustainable livestock farming. Following both precision and usability criteria, two main C sink databases covering the Andalusian region (S Spain) were developed from the Spanish Land Parcel Identification System (SIGPAC, coarse resolution) and the Spanish Information System on Land Cover (SIOSE, finer resolution) land use classes. Particular C sink factors based on growth rates for individual plant species were associated with detailed vegetation maps and, further, were linked to Land Use and Covers cartography across the region. In addition, eight ruminant farms were exhaustively studied in situ and used as a control. Results were compared with the obtained through the application of the developed C sink databases, and with the commonly used Petersen methodology. The sink capacity of vegetation associated with farms varied from 0.25 to 1.37 t CO2 ha−1 year−1, depending on the plant species composition and abundance. All the approaches showed significant differences from the control. C sink values were significantly higher when applying SIGPAC-based C sink database to farms, while values from the SIOSE and Petersen methodology approaches provided more moderate values, closer to the control. SIGPAC and Petersen approaches showed higher usability but presented lower precision due to a poor definition of plant cover. SIOSE-based C sink database provided suitable values able to be adapted to reality and used by farmers. In this regard, further research efforts to improve the adjustment of results and ease of use are required. The present approach means a methodological advance in the estimation of the C sink capacity associated with pastoral livestock farms, able to be incorporated into the CF calculation in contrasted areas worldwide, in the frame of the ‘eco-schemes’ being recently under development through the EU CAP.

A Conceptual Characterization of Value Creation in Hospitality and Tourism Firms from a Complexity Perspective

Hospitality and tourism firms are suffering more than others the devastating effects of the COVID-19 pandemic. Its recovery will require designing and implementing innovative value creation strategies that are hard to imagine with the simplifying cause-and-effect analytical frameworks so widespread today. Building on the narrative of the firm as a open complex adaptive system and the literature reviewed in economics and management, this article approaches value creation from the paradigm of complexity science and provides a conceptual bases that would allow value practitioners incorporate more realistic and holistic elements to its analysis. Several implications are outlined that could result from the adoption of this perspective and that might encourage a change in the mindset of scholars and firms’ managers. Guidelines for deeper exploration of value creation from complexity are also provided that could open new avenues for theoretical and methodological advance.

HIV-1 diversity considerations in the application of the Intact Proviral DNA Assay (IPDA)

The Intact Proviral DNA Assay (IPDA) was developed to address the critical need for a scalable method for intact HIV-1 reservoir quantification. This droplet digital PCR-based assay simultaneously targets two HIV-1 regions to distinguish genomically intact proviruses against a large background of defective ones, and its application has yielded insights into HIV-1 persistence. Reports of assay failures however, attributed to HIV-1 polymorphism, have recently emerged. Here, we describe a diverse North American cohort of people with HIV-1 subtype B, where the IPDA yielded a failure rate of 28% due to viral polymorphism. We further demonstrate that within-host HIV-1 diversity can lead the IPDA to underestimate intact reservoir size, and provide examples of how this phenomenon could lead to erroneous interpretation of clinical trial data. While the IPDA represents a major methodological advance, HIV-1 diversity should be addressed before its widespread adoption as a principal readout in HIV-1 remission trials.