Quantum dots (QDs) are very small nanoparticles and are composed of hundreds to thousands of atoms. These semiconducting materials can be made from an element, such as silicon or germanium, or compounds such as cadmium sulphide (CdS) or cadmium selenide (CdSe). The colour of these small particles does not depend on the type of semiconducting material from which the dots are made, but rather on its diameter. Besides, ODs attract the most attention because of their unique visual properties. Therefore, these are used in all kinds of applications where precise control of coloured light is important. As these dots are of great importance in chemical, biological and medical applications, they can be designed to deliver anti-cancer drugs and direct them to specific areas of the body. Therefore, with this technique, the harmful side effects of chemical treatments can be reduced. It is possible to examine and study the properties of these nanomaterials and make sure they are analyzed using some scientific devices and techniques, the most important of which are: transmittance electron microscopy (TEM), scanning electron microscopy (SEM), atomic forces microscopy (AFM) with dielectrics, and X-ray diffraction (XRD). This chapter opens horizons towards knowing what quantum dots are and their unique properties, as well as methods of preparation and then placing our hands on the chemical, and biological applications of these dots.