This paper primarily focuses on the effect of feed to inoculum ratio on biogas digestion; and
outlines the various feeds, inoculums, and synergistic effects of the combination of inoculums by
referring to the literature. The Start-up of an anaerobic digestion system is highly critical and
pivotal for the successful operation of an anaerobic digester. For this purpose, a certain amount of
inoculum is added to the digester along with the substrate to provide the necessary
microorganisms to initiate the digestion process. The ratio and the type of inoculum used
substantially affect the rate of biodegradation and the lag time. The degradation of substrate
depends on the concentration of microorganisms. When food waste was considered as the feed it
was found that factors such as waste oil content, the addition of alkaline buffer, particle size,
organic loading rate had a considerable effect on the feed to inoculum ratio. Studies considering
some other feeds such as animal by products from piggery slaughterhouses, poultry
slaughterhouse wastes, agro-industrial waste, anaerobically digested sludge obtained from
municipal wastewater, and dewatered digestate cake were also compared. Hence, the objective of
this study is to offer an integrated view of the appropriate feed and the inoculum under the effect
of various other essential parameters. The major performance indicators from this study were
found to be particle size of inoculum, organic loading rate, the addition of alkaline buffer, F/I
ratio, and structure of inoculum