ATPase Effects on pro-nutrients-mTOR release long fatty acids chains which under mitochondrial phospholipase, synthase & synthetase effects form three ROR-alpha, beta, gamma isoform

RORs isoforms are so active biological molecules in lipid metabolism and in fat biosynthesis, that strongly dependent on and regulated by OPA1 mitochondrial genes and its active mitochondrial enzymes where each of mitochondrial enzyme (phospholipase, synthase, and synthetase) is responsible for its own ROR isoform {phospholipase responsible for ROR-alpha synthesis, synthase responsible for ROR[1]beta synthesis, and synthetase responsible for ROR-gamma synthesis} for acting and functioning the long fatty acids molecules “which produced from the effects of ATPase and COX enzyme on lipid molecules which accompanied and associated with absorbed nutrient molecules (pro-lipo-nutrient -mTOR molecules) “, and then will follow its own pathway in fatty and amino acids biosynthesis, in active anti[1]inflammations biosynthesis, and then will follow its own functions in original cells proliferations.

Toll-like receptor-4 synthesis is regulated by JNK signaling by three glucocorticoids isoforms and by three interferons isoforms, also its advantages in activities depending on the Containment from Arg, proline and hydrophobic amino acids in its compositions

Toll-like receptor-4 (TLR4), synthesis is regulated by JNK signaling , by three glucocorticoids isoforms, and by the three interferons isoforms, also depending on avaliablities of LPS & on long fatty acids chains with Arg and proline availabilities For performing and running the mitochondrial oxidative processes for producing fatty-acyl-CoA-synthetase followed by fatty-acyl-CoA-synthase followed by fatty-acyl-CoA-phospholipase productions for linear TLR4 active beta-subunits which will be transformed into TLR4-alpha upon phospholipase effects , which will follow phosphorylation process (alpha-oxidations) for generate Guanosine triphosphate cyclohydrolase (GTP-Chase) subunits which supposed to contain specific hydrophobic amino acids including Arg, Tyr, leu, proline…. Etc, which is the rate-limiting enzyme for tetrahydrobiopterin (BH4) synthesis, which is essential for inducible iNOS from fatty-acyl-CoA-synthase upon the nitric oxidesynthase (NO-S) regulations effects. Proline can accelerate anabolic oxidative processes by OPA1 enzymes and provides site-specific flexibility for collagen synthesis in vivo, and also plays an necessary important roles in TLR4 and TGF-gamma/beta/& alpha synthesis and activities, where the presence of proline in IFN-gamma, in TLR4 genes and in IFN-beta will accelerate oxidative OPA1 anabolic processes and direct the flow of biological processes to proliferations of plasma-membranes , collagen synthesis and blood platelets biosynthesis. Vitamin E & K-dependent protein C are the key components of anticoagulant serine protease, And therefore vit E and vit k are providing specific advantages to TLR4 synthesis and modulated first in vivo as proper fatty-acyl-CoAsynthetase subunits (gamma-subunits) then modified fatty-acyl-CoA-synthase subunits upon synthase effects on gamma-subunits for producing IL-beta upon which will promote linear TLR4 upon both synthase and phospholipase effects for starting proliferation stepes started by catalyzing Arg for producing GTP-Chase and citrulline which the main basis for Erythropoietin productions , for Plasma membrane synthesis… etc. Both IFN-beta and glucocorticoid-beta are designed anti-inflammatory subunits are depending on each others and on the activities of OPA1-synthase enzyme for producing the long fatty acyl-CoA-synthase (Beta-subunit) with specific compositions and sequences from amino acids which can determine their advantages in immunity functions eg their containment of tyr, proline, Arg, gly.. etc, where, Both glucocorticoid-beta and IFN-beta are able to recover each others in their different tissues.

Quantification of IgG specific antibodies in recovered COVID -19 patients and its association with disease severity

Background: Serological tests for anti-SARS-CoV-2 antibodies can provide valuable information in epidemiologic and public health research. However, the data is limited on serological response post COVID-19 infection. Objective: To investigate the use of ARCHITECT SARS CoV-2 IgG II Quantitative assay to quantify the IgG antibodies in recovered COVID-19 infections. Methods: Abbott SARS-CoV-2 IgG assays were performed using the ARCHITECT Quantitative chemiluminescent microparticle immunoassay to detect IgG against SARS-CoV-2 S1 receptor binding domain (RBD) in serum and plasma. Patients having IgG concentrations above ≥50 AU/mL were defined as positive. We analyzed 333 COVID-19 patients who were tested negative by RT-PCR not more than three months after testing positive. Results: The mean age of the population was 34.3 years and 95% were male. About 93% of the patients had mild disease and rest had moderate disease. The mean IgG levels in the entire cohort was 1860.27 AU/mL, the lowest and highest value detected by ARCHITECT SARS CoV-2 IgG II Quantitative Assay (Abbott Laboratories, USA) were 39.8 AU/mL and 43657.4 AU/mL, respectively. While patients with moderate COVID-19 disease had numerically higher IgG levels than patients with mild disease, the difference was not statistically significant (1787.7 AU/mL vs. 2865.39 AU/mL, p=0.21). In 97% of the patients, IgG antibodies could be detected up to 95 days after testing negative on RT-PCR. Conclusion: Patients with moderate COVID-19 disease may develop higher IgG antibodies than in patients with mild disease. Antibodies can be detected in majority of recovered individuals for more than 90 days which may be valuable in developing future vaccination strategy.

Time scale of the growth progress in bacterial cultures: a self-consistent choice

An empirical model describes the behavior of an ideal microbial culture that mimics the real one. The predicted growth curve, log[N(t)], includes the onset tail of the observed sigmoid trend, while the latency phase that precedes the cell duplication is a time gap, which does not coincide with the so-called lag phase of other models. Unsolved remains the issue of the time scale: does the origin of the time scale of the experimenter coincide with that of the microbial culture? The correlation between duration of latency phase and maximum slope of the growth trend allows determination of a “true” starting point of the growth progress as the origin of the time scale of the microbial culture. Rigid shifts in the time scale of the experimenter allow alignment of the growth trends of real microbial cultures (in excess substrate, in the same medium and at the same temperature), no matter the starting population density. The time scale of the bacterial culture allows a consistent evaluation of the extent of the latency phase that can include a “negative” time span, which is supposed to deal with some basal cell metabolism not aimed at duplication. Some examples, dealing with real psychrotrophic bacterial cultures, support the model and perfect the prediction of the extent of the latency phase at various temperatures.

Chlamydia trachomatis IgM and gG antibodies and associated risk factors of among positive and negative HIV women attending consultation at the Mbouo-Banjoun Presbyterian Hospital West Region of Cameroon

Background:Chlamydia trachomatis (CT) infection in the genitourinary tract is the most prevalent bacterial sexually transmitted disease (STD) worldwide. Genital chlamydial infection has a huge impact on sexual and reproductive health, and it is very common in developed and developing countries. This study aimed to determine the seroprevalance and risk factors for C. trachomatisinfection in women seeking medical care in the locality of Mbouo-BanjounWest Region of Cameroon. Methods: We conducted a cross-sectional hospital based study from November 2016 to June 2017 in which we recruited 204 consenting women aged 18 to 55 years. A questionnaire was administered to study participants and potential risk factors for Chlamydia exposure sought. Venous blood was collected and serum from each participant analysed for C. trachomatis infection as evidenced by positive anti-C. trachomatisIgG and IgM antibodies detected using the Sandwich Enzyme-Linked ImmunoSorbent Assay (ELISA) technique. The proportion of anti-C. trachomatis antibody was calculated and predictors of C. trachomatis infection analysed by univariate and multivariate regression. Epi-Info 7 was used for statistical analyses. A p < 0.05 was considered significant in all analyses. Results: The seroprevalence of anti-C. trachomatisantibodies (IgM or IgG) was found to be62.25% [127/204]. Among seropositive women, 37.15% [77/204] were seropositive for IgG antibodies while 47.54% [97/204] were seropositive for IgM antibodies and 23.04% [47/204] where seropositive for both IgM and IgG antibodies. Among the risk factors evaluated, marital status (P= 0.03) and knowledge of Chlamydia (P= 0.001) were observed to be an independent risk factor of C. trachomatisinfection. Conclusions: Our findings suggest recent C. trachomatisexposure is high in our study population, and may constitute a significant risk factor for, ocular and pulmonary infection in new born child, infertility to women. Education and screening of HIV-positive individuals and pregnant women for C. trachomatisinfection may be important primary prevention strategies in this population.

Identification of Lactic Acid Bacteria Isolated from Local Commercial Yahe (Fermented Caridean-Shrimp) Products

Fermentation was used since ancient times as an easy method of food preservation, which also maintains and/or improves the nutritional and sensory properties of food. A research as aimed at identifying strain of lactic acid bacteria (LAB) from fermented caridean-shrimp, which properties suitable for starter cultures in food fermentation. A total of 18 LAB stains were obtained from ten different samples, in each sample consisted of commercial LAB strain that isolated from ten samples of caridean-shrimp. The LAB strains from ten samples were screened for resistance to biological barriers (acid and bile salts), and the three most promising strains were selected. The three bacteria strains were isolated from samples of caridean[1]shrimp and were characterized by the API 50 CHL system of identification. Three lactic acid bacteria species were identified and included Lactobacillus plantarum, and Lactobacillus acidophilus. Strain Y’11b,2, Y’11e,2, Y’85,1, which showed probiotic characteristics reducing cell growth of cancer, could be suitable as a starter culture for food fermentation because of its strong acid production and high acid tolerance. This is the first report to describe bacteria, isolated from caridean[1]shrimp, Lactobacillus Plantarum (Y’11b,2, Y’11e,2) and Lactobacillus acidophilus (Y’85,1) which have the probiotic characteristics and the acid tolerance needed for its use as a starter culture in food fermentation.

Moving ahead in HCV diagnostics: Need for the introduction of HCV core antigen assay in low and middle-income countries

Hepatitis C virus (HCV) infections are associated with significant morbidity and mortality globally. The diagnosis of HCV is primarily based on indirect serological assays such as enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CIA), and rapid diagnostic tests to detect HCV antibodies. Direct tests detect/quantify components of HCV virions, such as HCV ribonucleic acid (RNA) (nucleic acid test or nucleic acid amplification test [NAT]) and HCV core antigen (HCVcAg). The HCVcAg assay (CIA, Abbott ARCHITECT) is an immune assay used for the quantitative determination of the HCVcAg. This test is simple and fast with the potential to be incorporated into diagnostic guidelines and be used in combination with anti-HCV (CIA) as an effective screening test. HCVcAg can also be used as a potential biomarker for treatment initiation and monitoring patients to assess the treatment response. Apart from this, the scope for implementation of the HCVcAg assay in resource limited setting lies in screening of immune compromised patients where anti-HCV serology is not dependable. However, concerns related to lower sensitivity compared to HCV RNA do exist. Nevertheless, the HCVcAg assay can make a significant difference in the measures taken for the control and eradication of hepatitis C and its complications in India.

Rapid diagnosis of acute respiratory infections by multiplex endpoint PCR technology

Introduction: The multiplex endpoint PCR technology offers a number of potential advantages, results are available in a matter of hours rather than days, the extreme sensibility facilitates detection of even minutes the amounts of pathogen DNA in clinical samples and the test is not significantly affected by prior administration of antibiotics. Aim: The aim of this work was to rapidly identify the antibiotic resistance the monitoring of pathogen growth at the patients admitted in Hospitalization Intensive Care Unit, with the diagnosis of Community Acquired Pneumonia, (CAP). Method: The Analyzer Unyvero™ Pneumonia Application was used in detection of pneumonia associated pathogens and their antibiotic resistance genes using the Pneumonia Unyvero™ System, following PCR pathogen species with sequencing of the amplified microbial DNA. Results: The main pathogens of community acquired pneumonia from the cohort study,36 cases, (20 males in mean age 35-66 years and 16 females in mean age 40-55 mean years), were Streptococcus pneumonia, (16 cases), Staphilococcus aureus, (10 cases), Klebsiella pneumonia (5 cases) and other important agents were “atypical”, such as Haemophilus Influenzae, Chlamidophilapneumonie and Moraxelacataralis. A case with Acinetobacter baumani and Proteus Sp. was also widely resistance to mefA gene / ermB gene as all cases of analyzed. The more frequency of genes resistant (29 cases) are ermA gene / ermC / ermB for Staphilococcus aureus and the gene tem+shv / gene / ctx-M with the Chromosomal mutation (7 cases), as gyrA83_87 Ecoli / Pseu for Klebsiella pneumonia agents. Also, most resistance antibiotics were Makrolides, (29 cases and Lincosamides (6 cases) and these cases have had the chromosomial integrates. The most resistance microbe, Pseudomonas aeruginosa (1 case), has been registered as multi drugs resistance [MDR]*. Conclusion: The Unyvero™ results have been available 2 days before the primary microbiology report and 3 days before the final confirmation results, obtained by microbiology culture. The Unyvero Analyzer only provides rapid data to support the therapeutic decision of currant medic.

The role of neutrofiles and by macrophages in the pathogenesis of inflammatory and I post inflammatory episodes : Major tissue damage may be inflicted by a synergy among their toxic pro inflammatory agents

In his manuscript we summarize the role played by the variety of pro inflammatory agents generated by neutrofiles and by macrophages and how these cells may act in synergy to injure human and tissues in various inflammatory and in post inflammatory conditions. Screening the vast published literature, we found that most of papers published on inflammation, tend to stress only the role played by a very few pro inflammatory agents as key agents in inflammation. This is unreasonable to try to understand how leukocytes kill microbial cells and injure mammalian cells in various medical conditions. This is since the PMNs and macrophages produce numerous inflammatory agents.