Deactivation of Escherichia coli
 with different volumes in drinking water using cold atmospheric plasma

Cold plasma-liquid interaction becomes a growing interdisciplinary area of research involving plasma physics, fluid science, and chemistry. Plasma-liquid interaction has gained more interest over the last many years due to its potential applications in different fields. Cold atmospheric plasma jet is an emerging technology for surface drinking water treatment to improve quality and surface modification that is chemical-free and eco-friendly. Cold plasma treatment of water samples results in changes in turbidity, pH, and conductivity and in the formation of reactive oxygen and nitrogen species (RONS). As a result, plasma-activated water has a different chemical composition than water and can serve as an alternative technique for microbial disinfection. CAPJ has been generated by a high voltage 5 kV and a high frequency 19.56 kHz power supply. The discharge has been characterized by an optical method. To characterize the cold atmospheric pressure argon plasma jet, discharge plume temperature, and electron rotational and vibrational temperature have been determined. Cold atmospheric argon plasma jet produced at atmospheric condition contains high energetic electrons, ions, UV radiation, reactive oxygen, and nitrogen species named as cold plasma which has a wide range of applications in the biomedical industry, as well as in water treatment. Nowadays, researches have been carried out on ozonation through plasma jet interaction with surface drinking water. In this paper, we compare the change in physical and chemical parameters of surface water used for drinking purposes. The significant change in the physical parameters such as pH, turbidity, and electrical conductivity was studied. In addition, the significant changes in the concentration and absorbance of nitrate, ferrous, and chromium ions with respect to treatment time were studied. Our results showed that plasma jet interaction with surface drinking water samples can be useful for the improvement of water quality and an indicator for which reactive species play an important role in plasma sterilization.

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Assessment of Mutations Induced by Cold Atmospheric Plasma Jet Treatment Relative to Known Mutagens in Escherichia coli

Mutagenesis ◽

10.1093/mutage/geab030 ◽

2021 ◽

Author(s):

Bethany L Patenall ◽

Hollie J Hathaway ◽

Maisem Laabei ◽

Amber E Young ◽

Naing T Thet ◽

...

Keyword(s):

Escherichia Coli ◽

Dna Damage ◽

Dna Fragmentation ◽

Ames Test ◽

Plasma Jet ◽

Atmospheric Plasma ◽

Nitrogen Species ◽

Cold Atmospheric Plasma ◽

X Irradiation ◽

Mutagenic Effects

Abstract The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on Escherichia coli were assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, that did not resemble that for UV implying that CAP produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences.

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Sub-lethal exposure to cold atmospheric plasma in vitro induces changes in bacterial antibiotic resistance profiles. A pilot study.

International Journal of Infection Control ◽

10.3396/ijic.v16i1.004.20 ◽

2019 ◽

Vol 16 (1) ◽

Author(s):

Melina E. Martínez-Barrera ◽

Jaime Bustos Martinez ◽

Leonor Sánchez-Pérez ◽

Aída Hamdan-Partida ◽

A Enrique Acosta-Gio

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Methicillin Resistant Staphylococcus Aureus ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma ◽

Bacterial Antibiotic Resistance ◽

Exposure To Cold

To evaluate the effect of sub-lethal exposure to cold atmospheric plasma (CAP) on their antibiotic resistance, Methicillin Resistant Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa, Escherichia coli, Streptococcus mutans, and Candida albicans were exposed in vitro to a commercially available CAP. This antimicrobial CAP inhibited growth but changed survivors’ antibiotic resistance.

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The effect of cold atmospheric plasma and linalool nanoemulsions against Escherichia coli O157:H7 and Salmonella on ready-to-eat chicken meat

LWT ◽

10.1016/j.lwt.2021.111898 ◽

2021 ◽

pp. 111898

Author(s):

Cid R. González-González ◽

Olaoniye Labo-Popoola ◽

Gonzalo Delgado-Pando ◽

Katerina Theodoridou ◽

Olena Doran ◽

...

Keyword(s):

Escherichia Coli ◽

Chicken Meat ◽

Atmospheric Plasma ◽

Escherichia Coli O157 ◽

Cold Atmospheric Plasma

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Microbiological quality of residues from drinking water preparation

Water Science & Technology ◽

10.2166/wst.1995.0565 ◽

1995 ◽

Vol 31 (5-6) ◽

pp. 75-79 ◽

Cited By ~ 5

Author(s):

M. Würzer ◽

A. Wiedenmann ◽

K. Botzenhart

Keyword(s):

Waste Water ◽

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Drinking Water ◽

Water Treatment ◽

Pathogenic Bacteria ◽

Waste Water Treatment ◽

Microbiological Quality ◽

Detection Limits

In Germany the application of procedures such as flocculation and filtration in the preparation of drinking water results in the annual production of an estimated 500,000 t of sediments and sludges. Some of these residues have a potential for being reused, for example in agriculture, forestry, brickworks or waste water treatment. To assess the microbiological quality of residues from waterworks methods for the detection of enterobacteria, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Legionella, poliovirus, Ascaris suis eggs and Cryptosporidium have been evaluated regarding their detection limits and were applied to various residues from German waterworks. Results show that sediments and sludges may contain pathogenic bacteria, viruses and protista. When residues from waterworks are intended to be reused in agriculture or forestry the microbiological quality should therefore be considered.

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Targeting Protective Catalase of Tumor Cells with Cold Atmospheric Plasma- Activated Medium (PAM)

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170801103708 ◽

2018 ◽

Vol 18 (6) ◽

pp. 784-804 ◽

Cited By ~ 18

Author(s):

Georg Bauer

Keyword(s):

Control System ◽

Singlet Oxygen ◽

Tumor Cells ◽

Plasma Potential ◽

Atmospheric Plasma ◽

Antitumor Action ◽

Active Principle ◽

Reaction Products ◽

Cold Atmospheric Plasma

Background: Application of cold atmospheric plasma to medium generates “plasma-activated medium” that induces apoptosis selectively in tumor cells and that has an antitumor effect in vivo. The underlying mechanisms are not well understood. Objective: Elucidation of potential chemical interactions within plasma-activated medium and of reactions of medium components with specific target structures of tumor cells should allow to define the active principle in plasma activated medium. Methods: Established knowledge of intercellular apoptosis-inducing reactive oxygen/nitrogen species-dependent signaling and its control by membrane-associated catalase and SOD was reviewed. Model experiments using extracellular singlet oxygen were analyzed with respect to catalase inactivation and their relevance for the antitumor action of cold atmospheric plasma. Potential interactions of this tumor cell-specific control system with components of plasma-activated medium or its reaction products were discussed within the scope of the reviewed signaling principles. Results: None of the long-lived species found in plasma-activated medium, such as nitrite and H2O2, nor OCl- or .NO seemed to have the potential to interfere with catalase-dependent control of apoptosis-inducing signaling of tumor cells when acting alone. However, the combination of H2O2 and nitrite might generate peroxynitrite. The protonation of peroxnitrite to peroxynitrous acid allows for the generation of hydroxyl radicals that react with H2O2, leading to the formation of hydroperoxide radicals. These allow for singlet oxygen generation and inactivation of membrane-associated catalase through an autoamplificatory mechanism, followed by intercellular apoptosis-inducing signaling. Conclusion: Nitrite and H2O2 in plasma-activated medium establish singlet oxygen-dependent interference selectively with the control system of tumor cells.

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The Application of the Cold Atmospheric Plasma-Activated Solutions in Cancer Treatment

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170731115233 ◽

2018 ◽

Vol 18 (6) ◽

pp. 769-775 ◽

Cited By ~ 13

Author(s):

Dayun Yan ◽

Jonathan H. Sherman ◽

Michael Keidar

Keyword(s):

Cancer Treatment ◽

Atmospheric Plasma ◽

Current Status ◽

Cold Atmospheric Plasma ◽

Anti Cancer ◽

Long Time ◽

Key Topics ◽

The Common

Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment, PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine. Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in past years. The approaches to make strong and stable PAS are also summarized.

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