1. Rats previously starved for 24hr. were separately given by intraduodenal injections 0·5ml. of a dispersion containing 10mg. of sodium taurocholate, with 50mg. of glycerol 1,3-dioleate 2[1−14C]-palmitate, glycerol 1,2-dioleate 3[1−14C]-palmitate, a mixture of [1−14C]palmitic acid and triolein, or a mixture of [1−14C]-palmitic acid and oleic acid. 2. At the end of 30min., the net amounts, and the radioactivity, of the neutral-lipid components recovered from the intestinal lumen and mucosa, and the position of the labelled palmitic acid in the mucosal triglycerides, were determined. 3. When glycerol 1,3-dioleate 2[1−14C]-palmitate was administered, most of the labelled acid was retained in the di- and monoglycerides of the lumen; the triglycerides were the major components containing the radioactivity in the mucosa and 75–80% of the labelled acid was located at the β-position of these triglycerides. 4. When glycerol 1,2-dioleate 3[1−14C]-palmitate was administered, the labelled acid was readily split off in the lumen and virtually no radioactivity could be traced in the monoglyceride fraction; in the intestinal mucosa, triglycerides were again the chief components containing most of the radioactivity, and 80–85% of the labelled acid was esterified at the outer positions of the glycerol. 5. When [1−14C]palmitic acid mixed with triolein was administered, the concentrations of free fatty acids increased markedly in the intestinal lumen and mucosa, and 80–88% of the radioactivity of the mucosal triglycerides was located at the outer positions of the glycerol. 6. When [1−14C]palmitic acid mixed with oleic acid was administered, the labelled acid accumulated in the lumen as well as in the cell, and it was randomly incorporated into all three positions of the mucosal triglycerides.
Application of Sebum Lipidomics to Biomarkers Discovery in Neurodegenerative Diseases