Characterization of endogenous fluorescence in nonsmall lung cancerous cells: A comparison with nonmalignant lung normal cells

Background: Paclitaxel and spirulina when administered as nanoparticles, are potentially useful. Methods: Nanoformualtions of Paclitaxel and Spirulina for gastric cancer were formulated and optimized with Central composite rotatable design (CCRD) using Response surface methodology (RSM). Results: The significant findings were the optimal formulation of polymer concentration 48 mg, surfactant concentration 45% and stirring time of 60 min gave rise to the EE of (98.12 ± 1.3)%, DL of (15.61 ± 1.9)%, mean diameter of (198 ± 4.7) nm. The release of paclitaxel and spirulina from the nanoparticle matrix at pH 6.2 was almost 45% and 80% in 5 h and 120 h, respectively. The oral bioavailability for the paclitaxel spirulina nanoparticles developed is 24.0% at 10 mg/kg paclitaxel dose, which is 10 times of that for oral pure paclitaxel. The results suggest that RSM-CCRD could efficiently be applied for the modeling of nanoparticles. The paclitaxel and spirulina release rate in the tumor cells may be higher than in normal cells. Paclitaxel spirulina nanoparticle formulation may have higher bioavailability and longer sustainable therapeutic time as compared with pure paclitaxel. Conclusion: Paclitaxel-Spirulina co-loaded nanoparticles could be effectively useful in gastric cancer as chemotherapeutic formulation.

Download Full-text

Controllable Fabrication and Characterization of Gold Nanoparticles: Enhanced Therapeutic Approaches in Cancer

Open Journal of Science and Technology ◽

10.31580/ojst.v2i1.934 ◽

2019 ◽

Vol 2 (1) ◽

pp. 26-28

Author(s):

Aqsa Ikram ◽

Yasir Jamil ◽

Tahira Ahmad ◽

Hira Ikram ◽

Amna Ameen ◽

...

Keyword(s):

Well Being ◽

Nano Particles ◽

Cell Labeling ◽

Therapeutic Approaches ◽

Laser Hyperthermia ◽

Seed Growth ◽

Gold Nano Particles ◽

Controllable Fabrication ◽

Cancerous Cells

Energy trans-ducting gold nano-particles, with well-being physical, chemical and optical properties for utilization in different types of cancer therapy can be prepared through chemical seed growth reduction method. Gold nano particle is a umbrella term have a variety of size and shape. More regular gold nano-particles can be obtained by reduction of HAuCl4, selection of chemicals and parameters of growth medium are set in accordance with the required category of nano-particles. Nano-particles have longest dimension of 100 nm. Therapeutic treatments like photo-imaging, gene delivery, drug delivery, cell labeling and photo thermal therapy can be done by gold nano particles. They reduce the side effects of conventional therapy due to high target specificity and absorb and scatter specific wavelength of laser. Hyperthermia in cancerous cells is generated without damaging surrounding healthy tissues, cause the cell death by necrosis and apoptosis. The overall morphology of gold nano particles can be characterized by SEM and TEM.

Download Full-text

Is the effect of Decitabine limited to hypomethylation and DNMTs?

10.21203/rs.2.13184/v1 ◽

2019 ◽

Author(s):

Sina Dalvand ◽

Amin Namdari ◽

Ashraf Alemi ◽

Mohammad Hassan Meshkibaf ◽

Sam Setayesh ◽

...

Keyword(s):

Gene Expression ◽

Mrna Expression ◽

Histone Deacetylases ◽

Dna Methyltransferase ◽

Chromatin Condensation ◽

Dna Demethylation ◽

Histone Tails ◽

Normal Cells ◽

Before And After ◽

Cancerous Cells

Abstract Background: Histone modifications play a crucial role in chromatin structure. Among enzymes, which regulate these processes, histone deacetylases (HDACs) can remove acetyl groups from histone tails, thus increasing their interaction with DNA and leading to chromatin condensation. 5-Aza-2′-deoxycytidine (AZad) or Decitabine is a potent hypomethylating agent that incorporates into DNA and traps DNA methyltransferase in the form of a covalent protein–DNA adduct. Azad, not only change the gene expression through demethylation of the gene's promoter, but it also can change gene expression independently from DNA demethylation. So, the present study was to distinguish whether AZad in addition to inhibitory effects on DNA methyltransferase, can change HDAC3 and HDAC7 mRNA expression in NALM-6, HL-60 cancer cell lines. Methods: HL-60, NALM-6 and normal cells were cultured, and the treatment dose of the AZad was obtained (1µM) by the MTT test. Finally, HDAC3 and HDAC7 mRNA expression were measured by Real Time PCR in HL-60 and NALM-6 cancerous cells before and after treatment. In addition, HDAC3 and HDAC7 mRNA expression in un-treated HL-60 and NALM-6 cancerous cells were compared to the normal cells. Results: Our result revealed that expression of HDAC3 and HDAC7, in HL-60 and NALM-6 cells increases as compared to normal cells. After treatment of HL-60 and NALM-6 cells with AZad, HDAC3 and HDAC7 mRNA expression were decreased significantly. Conclusions: Our data showed, the effects of AZad are not limited to direct hypomethylation of DNMTs but it can indirectly affect other epigenetic factors, such as HDACs activity, through converging pathways. Keywords: HDAC3 ; HDAC7 ; HL-60; NALM-6 ; Decitabine ; AZad

Download Full-text

Vitamin C − protective role in oxidative stress conditions induced in human normal colon cells by label free Raman spectroscopy and imaging

10.1101/2021.04.12.439406 ◽

2021 ◽

Author(s):

Karolina Beton ◽

Beata Brozek-Pluska

Keyword(s):

Oxidative Stress ◽

Raman Spectroscopy ◽

Vitamin C ◽

Human Colon ◽

Protective Role ◽

Spectroscopic Methods ◽

Label Free ◽

Normal Cells ◽

Colon Cells ◽

Cancerous Cells

Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer, can detect it in advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise and unambiguous differentiation of healthy and cancerous samples. the most important advantage of Raman spectroscopy is ability to identify biomarkers that help in differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for: lipids, proteins, DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS by spectroscopic methods. Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal, ROS injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis.

Download Full-text

Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

Micromachines ◽

10.3390/mi11090832 ◽

2020 ◽

Vol 11 (9) ◽

pp. 832

Author(s):

Lexi Crowell ◽

Juan Yakisich ◽

Brian Aufderheide ◽

Tayloria Adams

Keyword(s):

Impedance Spectroscopy ◽

Cancer Cells ◽

Cancer Cell ◽

Cellular Response ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy ◽

Cell Behavior ◽

Drug Resistant ◽

Cancerous Cells

Electrical impedance spectroscopy (EIS) is an electrokinetic method that allows for the characterization of intrinsic dielectric properties of cells. EIS has emerged in the last decade as a promising method for the characterization of cancerous cells, providing information on inductance, capacitance, and impedance of cells. The individual cell behavior can be quantified using its characteristic phase angle, amplitude, and frequency measurements obtained by fitting the input frequency-dependent cellular response to a resistor–capacitor circuit model. These electrical properties will provide important information about unique biomarkers related to the behavior of these cancerous cells, especially monitoring their chemoresistivity and sensitivity to chemotherapeutics. There are currently few methods to assess drug resistant cancer cells, and therefore it is difficult to identify and eliminate drug-resistant cancer cells found in static and metastatic tumors. Establishing techniques for the real-time monitoring of changes in cancer cell phenotypes is, therefore, important for understanding cancer cell dynamics and their plastic properties. EIS can be used to monitor these changes. In this review, we will cover the theory behind EIS, other impedance techniques, and how EIS can be used to monitor cell behavior and phenotype changes within cancerous cells.

Download Full-text