Preparation and characterization of potato protein‐based microcapsules with an emphasis on the mechanism of interaction among the main components

2020 ◽  
Vol 100 (7) ◽  
pp. 2866-2872 ◽  
Author(s):  
Chenjie Wang ◽  
Tong Chang ◽  
Dongliang Zhang ◽  
Chengye Ma ◽  
Shanfeng Chen ◽  
...  
Keyword(s):  
Potato Protein ◽  
Mechanism Of Interaction ◽  
Main Components

scholarly journals A Concerted Action of UBA5 C-Terminal Unstructured Regions Is Important for Transfer of Activated UFM1 to UFC1

2021 ◽  
Vol 22 (14) ◽  
pp. 7390
Author(s):  
Nicole Wesch ◽  
Frank Löhr ◽  
Natalia Rogova ◽  
Volker Dötsch ◽  
Vladimir V. Rogov
Keyword(s):  
Cellular Localization ◽  
Molecular Mechanisms ◽  
Biochemical Characterization ◽  
Effective Interaction ◽  
Regulatory Region ◽  
Titration Calorimetry ◽  
Enzymatic Reactions ◽  
Cellular Processes ◽  
Mechanism Of Interaction

Ubiquitin fold modifier 1 (UFM1) is a member of the ubiquitin-like protein family. UFM1 undergoes a cascade of enzymatic reactions including activation by UBA5 (E1), transfer to UFC1 (E2) and selective conjugation to a number of target proteins via UFL1 (E3) enzymes. Despite the importance of ufmylation in a variety of cellular processes and its role in the pathogenicity of many human diseases, the molecular mechanisms of the ufmylation cascade remains unclear. In this study we focused on the biophysical and biochemical characterization of the interaction between UBA5 and UFC1. We explored the hypothesis that the unstructured C-terminal region of UBA5 serves as a regulatory region, controlling cellular localization of the elements of the ufmylation cascade and effective interaction between them. We found that the last 20 residues in UBA5 are pivotal for binding to UFC1 and can accelerate the transfer of UFM1 to UFC1. We solved the structure of a complex of UFC1 and a peptide spanning the last 20 residues of UBA5 by NMR spectroscopy. This structure in combination with additional NMR titration and isothermal titration calorimetry experiments revealed the mechanism of interaction and confirmed the importance of the C-terminal unstructured region in UBA5 for the ufmylation cascade.

scholarly journals Characterization of calcium oxide in root perforation sealer materials

2012 ◽  
Vol 23 (5) ◽  
pp. 539-546 ◽  
Author(s):  
Carlos Estrela ◽  
Manoel Damião Sousa-Neto ◽  
Orlando Aguirre Guedes ◽  
Ana Helena Gonçalves Alencar ◽  
Marco Antonio Hungaro Duarte ◽  
...  
Keyword(s):  
Calcium Oxide ◽  
Mineral Trioxide Aggregate ◽  
Chemical Compounds ◽  
Internal Diameter ◽  
X Ray ◽  
Final Sample ◽  
Materials Used ◽  
Main Components ◽  
White Mineral

Root perforation represents an undesirable complication that may lead to an unfavorable prognosis. The aims of this study were to characterize and to compare the presence of calcium oxide (CaO) on the chemical composition of materials used for root perforation therapy: gray and white mineral trioxide aggregate (MTA) and Portland cement (PC), gray MTA+5%CaO and gray MTA+10%CaO. The last two materials were analyzed to evaluate the increase of CaO in the final sample. CaO alone was used as a standard. Eighteen polyethylene tubes with an internal diameter of 3 mm and 3 mm in length were prepared, filled and then transferred to a chamber with 95% relative humidity and a temperature of 37ºC. The chemical compounds (particularly CaO) and the main components were analyzed by energy-dispersive X-ray microanalysis (EDX). EDX revealed the following concentrations of CaO: gray MTA: 59.28%, white MTA: 63.09%; PC: 72.51%; gray MTA+5%CaO: 63.48% and gray MTA+10%CaO: 67.55%. The tested materials presented different concentrations of CaO. Even with an increase of 5 and 10% CaO in gray MTA, the CaO levels found in the MTA samples were lower than those found in PC.

scholarly journals Characterization of hemicelluloses from leaves and tops of the CC 8475, CC 8592, and V 7151 varieties of sugarcane (Saccharum officinarum L

DYNA ◽  
2019 ◽  
Vol 86 (210) ◽  
pp. 98-107
Author(s):  
Luz Marina Flórez Pardo ◽  
Andrea González Córdoba ◽  
Jorge Enrique López Galán
Keyword(s):  
Nmr Spectroscopy ◽  
Saccharum Officinarum ◽  
Sodium Chlorite ◽  
Bioethanol Production ◽  
Valle Del Cauca ◽  
Main Components ◽  
Holocellulose Content ◽  
Potential Use

In this research, the types of hemicellulose that predominate in the leaves and tops of the three most cultivated varieties (CC 8475, CC 8592, V 7151) of sugarcane (Saccharum officinarum L.) in Valle del Cauca, Colombia were determined. Hemicellulose analyses were performed after delignification with sodium chlorite and extraction with 18% NaOH and 24% KOH containing 0.26 M NaBH4. The main components of hemicellulose were identified via FTIR and NMR spectroscopy, and monomeric sugars were identified via HPLC. Hemicellulose A composed of arabinoxylans, glucomannans and arabinogalactactans and hemicellulose B primarily composed of arabinoxylans were extracted. The hemicelluloses of the Colombian varieties were more stable against heat than those of the Venezuelan variety. The results of this project allowed discovery of the potential use of agricultural sugarcane residues for bioethanol production because they have a holocellulose content of more than 60%.

scholarly journals GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

2018 ◽  
Vol 5 (4) ◽  
pp. 79 ◽  
Author(s):  
Pilar A. Soledispa ◽  
José González ◽  
Armando Cuéllar ◽  
Julio Pérez ◽  
Max Monan
Keyword(s):  
Mass Spectra ◽  
Chemical Constituents ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Analytical Technique ◽  
Main Components ◽  
First Time

A preliminary chemical characterization of main components of ethanolic extract with dried rhizomes of Smilax domingensis Wid. that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. After sample derivatization 125 chemical compounds were registered by the equipment and from them, 35 different chemical components were characterized and reported for the first time from this part of the plant in our country. The results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.

scholarly journals Characterization and Cytotoxicity Assessment of the Lipophilic Fractions of Different Morphological Parts of Acacia dealbata

2020 ◽  
Vol 21 (5) ◽  
pp. 1814 ◽  
Author(s):  
Cátia S. D. Oliveira ◽  
Patrícia Moreira ◽  
Judite Resende ◽  
Maria T. Cruz ◽  
Cláudia M. F. Pereira ◽  
...  
Keyword(s):  
Gas Chromatography Mass Spectrometry ◽  
Detailed Characterization ◽  
Lipophilic Compounds ◽  
Acacia Dealbata ◽  
Forest Exploitation ◽  
Wood Extract ◽  
Cytotoxicity Assessment ◽  
Main Components ◽  
Long Chain

Acacia dealbata biomass, either from forest exploitation or from the management of invasive species, can be a strategic topic, namely as a source of high-value compounds. In this sense, the present study aimed at the detailed characterization of the lipophilic components of different morphological parts of A. dealbata and the evaluation of their cytotoxicity in cells representative of different mammals’ tissues. The chemical composition of lipophilic extracts from A. dealbata bark, wood and leaves was evaluated using gas chromatography-mass spectrometry (GC–MS). Terpenic compounds (representing 50.2%–68.4% of the total bark and leaves extracts, respectively) and sterols (60.5% of the total wood extract) were the main components of these extracts. Other constituents, such as fatty acids, long-chain aliphatic alcohols, monoglycerides, and aromatic compounds were also detected in the studied extracts. All the extracts showed low or no cytotoxicity in the different cells tested, demonstrating their safety profile and highlighting their potential to be used in nutraceutical or pharmaceutical applications. This study is therefore an important contribution to the valorization of A. dealbata, demonstrating the potential of this species as a source of high value lipophilic compounds.

Evaluation of Sunflower (Helianthus annuus L.) Hybrids Using Multivariate Statistical Analysis

Helia ◽  
2015 ◽  
Vol 38 (63) ◽  
pp. 175-187
Author(s):  
Natalija Markova Ruzdik ◽  
Ilija Karov ◽  
Sasa Mitrev ◽  
Biljana Gjorgjieva ◽  
Biljana Kovacevik ◽  
...  
Keyword(s):  
Seed Yield ◽  
Environmental Conditions ◽  
Agronomic Traits ◽  
Block Design ◽  
Principal Component ◽  
Original Data ◽  
Research Field ◽  
Multivariate Statistical ◽  
Main Components

AbstractEvaluation and characterization of sunflower hybrids using morphological, physiological and biochemical data are necessary and essential in sunflower breeding programs. The aim of this paper was to evaluate the productive possibilities of some sunflower hybrids grown in Macedonian environmental conditions. The experiment was carried out during the period 2013 and 2014 on the research field of the Faculty of Agriculture, “Goce Delchev” University – Shtip, in Ovche Pole locality, Republic of Macedonia. Total 20 sunflower hybrids were used as an experimental material. The trial was arranged as randomized complete block design (RCBD) with three replications. The average content of oil and oleic acid were 45.6 and 65.2%, respectively. The highest seed yield from all sunflower hybrids was obtained for the hybrid NLK12M144 (3,344 kg ha–1) and the lowest for hybrid NLK12S126 (2,244 kg ha–1). Cluster analysis classified the sunflower hybrids into four groups based on agronomic traits and seed yield. Most of the hybrids were included in cluster I and III (7 hybrids) followed by cluster IV. Using principal component analysis two main components have been extracted, with eigenvalue greater than one. They account for 72.99% of the variability in the original data. Only four hybrids had positive values for both main components (NLK12M144, NLK12S070, NLK12S125 and NLN12N011 DMR). The results from this study can be used to identify the best sunflower hybrids grown under Macedonian environmental conditions and to select the superior hybrids for future exploitation.

scholarly journals Synthesis and characterization of green tea (Camellia sinensis (L.) Kuntze) extract and its major components-based nanoflowers: a new strategy to enhance antimicrobial activity

RSC Advances ◽  
2017 ◽  
Vol 7 (70) ◽  
pp. 44303-44308 ◽  
Author(s):  
Ayşe Baldemir ◽  
N. Buse Köse ◽  
Nilay Ildız ◽  
Selen İlgün ◽  
Sadi Yusufbeyoğlu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Synthesis And Characterization ◽  
New Strategy ◽  
Main Components ◽  
First Time

In this study, for the first time, a novel organic–inorganic nanobio-antimicrobial agent called “nanoflowers” (Nfs) from Camellia sinensis (L.) Kuntze extracts and its main components were produced and the increase in the antimicrobial activity of Nfs was elucidated.

A Characterization of Cam Transmissions Through an Identification of Lumped Parameters

2006 ◽  
Author(s):  
Giuseppe Carbone ◽  
Chiara Lanni ◽  
Marco Ceccarelli ◽  
Giovanni Incerti ◽  
Monica Tiboni
Keyword(s):  
Dynamic Behaviour ◽  
Low Cost ◽  
Experimental Tests ◽  
Experimental Procedure ◽  
Operation Procedure ◽  
Alternative Testing ◽  
Lumped Parameters ◽  
Main Components ◽  
Easy Operation

In this paper, a numerical-experimental procedure is proposed for an identification of parameters in cam transmissions. Models with lumped parameters are defined specifically for cam transmissions. Experimental tests are carried out on main components of a cam transmission in order to estimate the values of mass, damping and stiffness lumped parameters through a low-cost easy-operation procedure. Experimental tests are also carried out in order to characterize the dynamic behaviour of a whole cam transmission. A comparison of numerical and experimental results is used in order to calibrate the values of lumped parameters. Experimental tests have been carried out by means of suitable test-beds for cams that have been built specifically at University of Brescia and at LARM in Cassino as alternative testing solutions.

Investigation of a Collagen-Chitosan-Hydroxyapatite System for Novel Bone Substitutes

2007 ◽  
Vol 330-332 ◽  
pp. 415-418 ◽  
Author(s):  
Xiao Liang Wang ◽  
Xu Dong Li ◽  
Xiao Min Wang ◽  
Jian Lu ◽  
Hui Chuan Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Substitutes ◽  
Nano Materials ◽  
Natural Polymers ◽  
Uniform Dispersion ◽  
Obvious Phase Separation ◽  
Ft Ir ◽  
Main Components

Collagen (Col) and chitosan (Chi) are both natural polymers and have received extensive investigation in recent years in the field of tissue engineering, but there are few reports on the introduction of hydroxyapatite (HA) into the Col-Ch system. In this study, based on the miscibility of these two polymers under proper condition, hydroxyapatite (HA) was synthesis in the Col-Chi system by in-situ co-precipitate method to give rise to a novel nanocomposite. The structural characterization of such Col-Ch-HA nano-materials was carried out by using FT-IR, XRD, SEM and TGA analyses with main components and Col-Chi samples used for comparison. It was found that there exist interactions between Col and Chi molecules. The nucleation and growth of inorganic phase occurs in the Col-Chi system and final products are uniform dispersion of nano-sized HA in the Col-Chi network without obvious phase separation. This novel nanocomposite would be a promising material for bone tissue engineering.

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