Characterization and Cytotoxicity Assessment of the Lipophilic Fractions of Different Morphological Parts of Acacia dealbata

Acacia dealbata biomass, either from forest exploitation or from the management of invasive species, can be a strategic topic, namely as a source of high-value compounds. In this sense, the present study aimed at the detailed characterization of the lipophilic components of different morphological parts of A. dealbata and the evaluation of their cytotoxicity in cells representative of different mammals’ tissues. The chemical composition of lipophilic extracts from A. dealbata bark, wood and leaves was evaluated using gas chromatography-mass spectrometry (GC–MS). Terpenic compounds (representing 50.2%–68.4% of the total bark and leaves extracts, respectively) and sterols (60.5% of the total wood extract) were the main components of these extracts. Other constituents, such as fatty acids, long-chain aliphatic alcohols, monoglycerides, and aromatic compounds were also detected in the studied extracts. All the extracts showed low or no cytotoxicity in the different cells tested, demonstrating their safety profile and highlighting their potential to be used in nutraceutical or pharmaceutical applications. This study is therefore an important contribution to the valorization of A. dealbata, demonstrating the potential of this species as a source of high value lipophilic compounds.

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Detailed characterization of bio-oil from pyrolysis of non-edible seed-cakes by Fourier Transform Infrared Spectroscopy (FTIR) and gas chromatography mass spectrometry (GC–MS) techniques

Journal of Chromatography B ◽

10.1016/j.jchromb.2017.05.014 ◽

2017 ◽

Vol 1058 ◽

pp. 47-56 ◽

Cited By ~ 6

Author(s):

Vatsala Sugumaran ◽

Shanti Prakash ◽

Emmandi Ramu ◽

Ajay Kumar Arora ◽

Veena Bansal ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Fourier Transform ◽

Infrared Spectroscopy ◽

Fourier Transform Infrared Spectroscopy ◽

Gas Chromatography Mass Spectrometry ◽

Detailed Characterization ◽

Bio Oil ◽

Edible Seed

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Chemical Components of Four Essential Oils in Aromatherapy Recipe

Natural Product Communications ◽

10.1177/1934578x1501000673 ◽

2015 ◽

Vol 10 (6) ◽

pp. 1934578X1501000 ◽

Cited By ~ 4

Author(s):

Sarin Tadtong ◽

Narisa Kamkaen ◽

Rith Watthanachaiyingcharoen ◽

Nijsiri Ruangrungsi

Keyword(s):

Essential Oils ◽

Volume Ratio ◽

Gas Chromatography Mass Spectrometry ◽

Chemical Components ◽

Chemical Markers ◽

Eucalyptus Oil ◽

Pine Oil ◽

Rosemary Oil ◽

Main Components

This study focused on characterization of the chemical components of an aromatherapy recipe. The formulation consisted of four blended essential oils; rosemary oil, eucalyptus oil, pine oil and lime oil (volume ratio 6: 2: 1: 1). The single and combination essential oils were identified by gas chromatography-mass spectrometry (GC-MS). The analysis of GC-MS data revealed that several components exist in the mixture. The five most important components of the blended essential oils were 1,8-cineole (35.6 %), α-pinene (11.1 %), limonene (9.6 %), camphor (8.4 %), and camphene (6.6 %). The main components of rosemary oil were 1,8-cineole (37.3 %), α-pinene (19.3 %), camphor (14.7 %), camphene (8.8 %), and β-pinene (5.5 %); of eucalyptus oil 1,8-cineole (82.6 %) followed by limonene (7.4 %), o-cymene (4.3 %), γ-terpinene (2.7 %), and α-pinene (1.5 %); of pine oil terpinolene (26.7 %), α-terpineol (20.50 %), 1-terpineol (10.8 %), α-pinene (6.0 %), and γ-terpineol (5.3 %); and of lime oil limonene (62.9 %), γ-terpinene (11.5 %), α-terpineol (7.6 %), terpinolene (6.0 %), and α-terpinene (2.8 %). The present study provided a theoretical basis for the potential application of blended essential oils to be used as an aromatherapy essential oil recipe. GC-MS serves as a suitable and reliable method for the quality control of the chemical markers.

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Detailed Componential Characterization of Extractable Species with Organic Solvents from Wheat Straw

International Journal of Analytical Chemistry ◽

10.1155/2017/7305682 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9

Author(s):

Yong-Chao Lu ◽

Yao Lu ◽

Zhao-Lin Lu ◽

Xian-Yong Wei

Keyword(s):

Wheat Straw ◽

Photoelectron Spectroscopy ◽

Energy Dispersive Spectrometry ◽

Gas Chromatography Mass Spectrometry ◽

Detailed Characterization ◽

X Ray ◽

Componential Analysis ◽

Transmission Electron ◽

Total Extractives

Componential analysis of extractives is important for better understanding the structure and utilization of biomass. In this investigation, wheat straw (WS) was extracted with petroleum ether (PE) and carbon disulfide (CS2) sequentially, to afford extractable fractions EFPE and EFCS2, respectively. Detailed componential analyses of EFPE and EFCS2 were carried out with Fourier transform infrared (FTIR) spectroscopy, gas chromatography/mass spectrometry (GC/MS), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), energy dispersive spectrometry (EDS), and electron probe microanalysis (EPMA). Total extractives were quantified 4.96% by weight compared to the initial WS sample. FTIR and GC/MS analyses results showed that PE was effective for the extraction of ketones and waxes derived compounds; meanwhile CS2 preferred ketones and other species with higher degrees of unsaturation. Steroids were enriched into EFPE and EFCS2 with considerable high relative contents, namely, 64.52% and 79.58%, respectively. XPS analysis showed that most of the C atoms in extractives were contained in the structures of C-C, C-COOR, and C-O. TEM-EDS and EPMA analyses were used to detect trace amount elements, such as Al, Si, P, S, Cl, and Ca atoms. Detailed characterization of extractable species from WS can provide more information on elucidation of extractives in biomass.

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Analysis of the core components of Alzheimer paired helical filaments A gas chromatography/mass spectrometry characterization of fatty acids, carbohydrates and long-chain bases

FEBS Letters ◽

10.1016/0014-5793(95)00486-s ◽

1995 ◽

Vol 366 (1) ◽

pp. 81-85 ◽

Cited By ~ 17

Author(s):

Warren J. Goux ◽

Santiago Rodriguez ◽

Dennis R. Sparkman

Keyword(s):

Mass Spectrometry ◽

Fatty Acids ◽

Gas Chromatography ◽

Paired Helical Filaments ◽

Gas Chromatography Mass Spectrometry ◽

The Core ◽

Chromatography Mass Spectrometry ◽

Core Components ◽

Long Chain

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Characterization of Waxy Material of the Giant Whitefly, Aleurodicus Dugesii, Using Scanning Electron Microscopy (SEM) and Capillary Gas Chromatography-Mass Spectrometry (CGC-MS)

Microscopy and Microanalysis ◽

10.1017/s1431927600007492 ◽

1997 ◽

Vol 3 (S2) ◽

pp. 121-122

Author(s):

T. P. Freeman ◽

D. R. Nelson ◽

J. S. Buckner ◽

R. L. Ruud ◽

C. L. Fatland

Keyword(s):

Electron Microscopy ◽

Leaf Surface ◽

Dorsal Surface ◽

Gas Chromatography Mass Spectrometry ◽

Male And Female ◽

Abaxial Leaf Surface ◽

Waxy Material ◽

Long Chain ◽

Scanning Electron

The giant whitefly is a pest, particularly of Hibiscus and sometimes avocados, in southern California. The insect is unique in that the nymphal stages produce copious quantities of waxy material differing in structure and chemical composition. Adults produce a waxy material (waxy particles) which covers them and surrounding surfaces, and the female also produces a waxy material with which she forms a circular trail of wax strands on the abaxial leaf surface.Waxy particles produced by male and female adults consisted of long-chain aldehydes and alcohols, largely 32 carbons in chain length. As waxy ribbons extruded from anterior abdominal wax plates, they are periodically broken off by the tibia and the resulting particles coat adults, nymphs and surrounding surfaces. The female has a second set of wax plates posteriorly on her abdomen which produce waxy strands. As her abdomen drags along the abaxial leaf surface during oviposition these strands break off forming a waxy trail camouflaging the eggs.The nymphs produce several types of waxy material. Two types are produced at the same time from 10 pores on the dorsal surface; waxy material is extruded as a filament on which a second waxy material curls off as extrusion occurs.

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Characterization of Nepeta viscida, N. nuda subsp. nuda and the Putative Hybrid N. × tmolea Essential Oils

Records of Natural Products ◽

10.25135/rnp.250.21.04.2033 ◽

2021 ◽

Vol 15 (5) ◽

pp. 388-395

Author(s):

Sema Çarıkçı

Keyword(s):

Gas Chromatography ◽

Essential Oils ◽

Gas Chromatography Mass Spectrometry ◽

Putative Hybrid ◽

Ionization Detector ◽

Natural Habitats ◽

Flame Ionization ◽

Aerial Parts ◽

Main Components

Nepeta viscida, N. nuda subsp. nuda, and their putative hybrid N. × tmolea were evaluated for their essential oils with chemotaxonomical aspect. Hybrid of N. x tmolea individuals were observed in the regions where the distribution of N. viscida and N. nuda subsp. nuda taxa were present, namely Dursunbey (Balıkesir) and Ödemiş (İzmir) natural habitats, respectively. The aerial parts of the taxa were hydrodistilled for 4 h using a Clevenger-type apparatus. The essential oils were analyzed both by gas chromatography-mass spectrometry (GC/MS) and gas chromatography flame ionization detector (GC-FID). The main components of the oils for the species collected were spathulenol, β-elemene, and 1,8-cineole, supporting the hybridization proposition.

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Intramolecular Proton Transfer in the Isomerization of Hydroxyacetone: A Detailed Characterization Based on Reaction Force Analysis and the Bond Fragility Spectrum

10.26434/chemrxiv.12049062 ◽

2020 ◽

Author(s):

Wallace Derricotte ◽

Huiet Joseph

Keyword(s):

Chemical Potential ◽

Reaction Force ◽

Intramolecular Proton Transfer ◽

Intermediate Energy ◽

Force Analysis ◽

Activation Energy Barrier ◽

Detailed Characterization ◽

Proton Transfers ◽

Reaction Force Constant

The mechanism of isomerization of hydroxyacetone to 2-hydroxypropanal is studied within the framework of reaction force analysis at the M06-2X/6-311++G(d,p) level of theory. Three unique pathways are considered: (i) a step-wise mechanism that proceeds through formation of the Z-isomer of their shared enediol intermediate, (ii) a step-wise mechanism that forms the E-isomer of the enediol, and (iii) a concerted pathway that bypasses the enediol intermediate. Energy calculations show that the concerted pathway has the lowest activation energy barrier at 45.7 kcal mol<sup>-1</sup>. The reaction force, chemical potential, and reaction electronic flux are calculated for each reaction to characterize electronic changes throughout the mechanism. The reaction force constant is calculated in order to investigate the synchronous/asynchronous nature of the concerted intramolecular proton transfers involved. Additional characterization of synchronicity is provided by calculating the bond fragility spectrum for each mechanism.

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