Detection and differentiation of Campylobacter jejuni and Campylobacter coli in broiler chicken samples using a PCR/DNA probe membrane based colorimetric detection assay

In this study, the conventional International Organization for Standardization (ISO) culture method was compared with the DuPont Qualicon BAX system, a high-throughput, rapid molecular assay that can be used to detect several bacterial species, including Campylobacter jejuni and Campylobacter coli in diverse sample types. Standard enrichment culture is a time-consuming process, taking up to 6 days to obtain a confirmed result. Rapid molecular assays have been developed that provide results within 24 h. Naturally contaminated samples from the poultry production chain were examined for the presence of Campylobacter spp. Samples from broiler chicken ceca (n = 100), fresh chicken carcass rinses (n = 60), and bootsocks (gauze sock walked through a broiler chicken house; n = 50) were enriched according to the ISO 10272 method in Bolton broth specifically designed to detect Campylobacter spp. in complex sample types. Samples were enriched without blood for use with the BAX system using the Campylobacter BAX kits for the detection of C. jejuni and C. coli. Samples also were directly plated onto modified charcoal cefperazone deoxycholate agar, and results were compared with those from the enriched samples for the ability to detect Campylobacter spp. Campylobacter spp. were isolated from 49% of samples with conventional enrichment cultures, from 48% with direct culture, from 68% with the BAX system and enrichment cultures, and from 62% with the BAX system used directly with samples. Overall, the BAX system detected more positive samples than did the conventional culture method and is an effective methodology for the rapid and reliable detection of Campylobacter spp. from diverse sample types.

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Comparison of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolated from Humans and Chicken Carcasses in Poland

Journal of Food Protection ◽

10.4315/0362-028x-71.3.602 ◽

2008 ◽

Vol 71 (3) ◽

pp. 602-607 ◽

Cited By ~ 30

Author(s):

ELŻBIETA ROŻYNEK ◽

KATARZYNA DZIERŻANOWSKA-FANGRAT ◽

DOROTA KORSAK ◽

PIOTR KONIECZNY ◽

SEBASTIAN WARDAK ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Campylobacter Jejuni ◽

Genetic Background ◽

Broiler Chicken ◽

Chicken Meat ◽

Campylobacter Coli ◽

Drug Resistant ◽

Stool Samples ◽

Campylobacter Infection ◽

Foodborne Infection

Campylobacter-associated gastroenteritis remains an important cause of morbidity worldwide, and some evidence suggests that poultry is an important source of this foodborne infection in humans. This study was conducted to analyze the prevalence and genetic background of resistance of 149 Campylobacter jejuni and 54 Campylobacter coli strains isolated from broiler chicken carcasses and from stool samples of infected children in Poland from 2003 through 2005. Nearly all isolates were susceptible to macrolides and aminoglycosides. The highest resistance in both human and chicken strains was observed for ciprofloxacin (more than 40%), followed by ampicillin (13 to 21%), and tetracycline (8 to 29%). Resistance to ampicillin and tetracycline rose significantly between 2003 and 2005. Slight differences in resistance between human and chicken isolates indicate that although chicken meat is not the only source of Campylobacter infection in our population, it can be involved in the transmission of drug-resistant Campylobacter strains to humans.

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Schnellnachweis von Campylobacter jejuni und Campylobacter coli aus Lebensmittelvoranreicherungen mittels Real-Time-PCR sowie immunomagnetischer Separation

Das Gesundheitswesen ◽

10.1055/s-2005-865579 ◽

2005 ◽

Vol 67 (03) ◽

Author(s):

A Mayr ◽

H Söllner ◽

R Zucker ◽

A Wieland ◽

A Notzon ◽

...

Keyword(s):

Real Time ◽

Campylobacter Jejuni ◽

Real Time Pcr ◽

Campylobacter Coli

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Campylobacter coli bacteraemia: how common is it?

BMJ Case Reports ◽

10.1136/bcr-2020-236634 ◽

2020 ◽

Vol 13 (12) ◽

pp. e236634

Author(s):

Sindhura Pisipati ◽

Adnan Zafar ◽

Yousaf Zafar

Keyword(s):

Liver Cirrhosis ◽

Campylobacter Jejuni ◽

Unusual Case ◽

Campylobacter Coli ◽

Decompensated Liver Cirrhosis ◽

The Past

Campylobacter species are known to cause enteritis. However, over the past 40–50 years, there have been reports of varying presentations, such as cellulitis, spondylodiscitis and bacteraemia. Of the Campylobacter species, Campylobacter jejuni is the most common culprit for causing bacteraemia, however, Campylobacter coli bacteraemia is becoming more prevalent. Here, we discuss an unusual case of C. coli bacteraemia in a patient with decompensated liver cirrhosis.

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Whole genome-based characterisation of antimicrobial resistance and genetic diversity in Campylobacter jejuni and Campylobacter coli from ruminants

Scientific Reports ◽

10.1038/s41598-021-88318-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Medelin Ocejo ◽

Beatriz Oporto ◽

José Luis Lavín ◽

Ana Hurtado

Keyword(s):

Genetic Diversity ◽

Antimicrobial Resistance ◽

Campylobacter Jejuni ◽

Resistance Genes ◽

Campylobacter Coli ◽

Whole Genome ◽

Northern Spain ◽

Sequence Alignments ◽

Phenotypic Data ◽

Wide Range

AbstractCampylobacter, a leading cause of gastroenteritis in humans, asymptomatically colonises the intestinal tract of a wide range of animals.Although antimicrobial treatment is restricted to severe cases, the increase of antimicrobial resistance (AMR) is a concern. Considering the significant contribution of ruminants as reservoirs of resistant Campylobacter, Illumina whole-genome sequencing was used to characterise the mechanisms of AMR in Campylobacter jejuni and Campylobacter coli recovered from beef cattle, dairy cattle, and sheep in northern Spain. Genome analysis showed extensive genetic diversity that clearly separated both species. Resistance genotypes were identified by screening assembled sequences with BLASTn and ABRicate, and additional sequence alignments were performed to search for frameshift mutations and gene modifications. A high correlation was observed between phenotypic resistance to a given antimicrobial and the presence of the corresponding known resistance genes. Detailed sequence analysis allowed us to detect the recently described mosaic tet(O/M/O) gene in one C. coli, describe possible new alleles of blaOXA-61-like genes, and decipher the genetic context of aminoglycoside resistance genes, as well as the plasmid/chromosomal location of the different AMR genes and their implication for resistance spread. Updated resistance gene databases and detailed analysis of the matched open reading frames are needed to avoid errors when using WGS-based analysis pipelines for AMR detection in the absence of phenotypic data.

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Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens

Antibiotics ◽

10.3390/antibiotics10050594 ◽

2021 ◽

Vol 10 (5) ◽

pp. 594

Author(s):

Sydney E. Schnur ◽

Raghavendra G. Amachawadi ◽

Giovanna Baca ◽

Sarah Sexton-Bowser ◽

Davina H. Rhodes ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Phenolic Compounds ◽

Enterococcus Faecalis ◽

Campylobacter Jejuni ◽

Bacterial Pathogens ◽

Klebsiella Oxytoca ◽

Bovine Mastitis ◽

Campylobacter Coli ◽

Foodborne Illnesses ◽

Phenolic Extract

Antimicrobial resistance in bacterial pathogens associated with bovine mastitis and human foodborne illnesses from contaminated food and water have an impact on animal and human health. Phenolic compounds have antimicrobial properties and some specialty sorghum grains are high in phenolic compounds, and the grain extract may have the potential as a natural antimicrobial alternative. The study’s objective was to determine antimicrobial effects of sorghum phenolic extract on bacterial pathogens that cause bovine mastitis and human foodborne illnesses. Bacterial pathogens tested included Escherichia coli, Salmonella Typhimurium, Campylobacter jejuni, Campylobacter coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Klebsiella oxytoca, Staphylococcus aureus, and Enterococcus faecalis. Antibacterial activities of sorghum phenolic extracts were determined by agar-well diffusion assay. Sorghum phenolic extract was added to the wells in concentrations of 0, 100, 200, 500, 1000, or 4000 µg/mL. The control wells did not receive phenolic extract. Plates were incubated for 18–24 h, and the diameter of each zone of inhibition was measured. The results indicated that sorghum phenolic extract had inhibitory effects on Staphylococcus aureus, Enterococcus faecalis, Campylobacter jejuni, and Campylobacter coli.

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