Biotechnological Approach to Cultivation of Rhododendron tomentosum (Ledum palustre) as the Source of the Biologically Active Essential Oil

Echinace from the Asteracea family has been among the top 10 marketed herbs in North America and Europe. So far, only a limited amount of Echinace originate from commercial cultivation. Echinacea purpurea has been commercially cultivated in the United States since the mid 1970s, in Russia since 1935, while in Europe since the 1960s. However, there has been no published information in the English language on methods of cultivation, expected yields, limiting factors, nutrient requirements, and the optimum plant development stage to obtain maximum yield and concentration of the biologically active substances for pharmaceutical and cosmetic processing. The objective of this investigation was, therefore, to study the general distribution of major nutrients and biologically active substances and the relationship between the two groups, and the pattern of the accumulation of cichoric acid, echnacoside, isobutylamides (tetraenes) and essential oil in commercially cultivated Echinacea purpurea. There was a significant variation in the content of essential oil, cichoric acid, and isobutylamides (tetraenes) in different organs and stages of the plant development. Similarly, the difference in the distribution of N, P, K, Ca, Mg, and Fe in different organs and developmental stages is demonstrated. The correlation between the content of the active substances and nutrients is demonstrated, while recommendations for an optimum harvesting stage and date are made.

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Comparative investigation of sesquiterpene components of essential oils originating from intact plants and hairy root chamomile cultures

GSC Advanced Research and Reviews ◽

10.30574/gscarr.2021.6.2.0016 ◽

2021 ◽

Vol 6 (2) ◽

pp. 028-049

Author(s):

Éva Szőke ◽

Éva Lemberkovics

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Hairy Root ◽

Folk Medicine ◽

Root Culture ◽

Hairy Root Culture ◽

Comparative Investigation ◽

Biologically Active

The importance of chamomile (Chamomilla recutita) inflorescence is widely known in classical and folk medicine, with the largest group of its effective constituents forming the essential oil (chamazulene, a-bisabolol, α-farnesene, trans-β-farnesene, spathulenol, cis/trans-en-in-dicycloethers). Among cultivated species, the Hungarian BK-2 contains more chamazulene in its essential oil than the German Degumil type, which is mainly cultivated for its a-bisabolol. Both components have important antiinflammatory activities. Wild populations can be easily distinguished from cultivated ones by their high amount of bisaboloides, particularly the flower of Hungarian Szabadkígyós wild type, which contained on average 48 % of the biologically active (-)-a-bisabolol. The population of Szabadkígyós has good salt tolerance which is important owing to global warming, because the proportion of saline areas is increasing worldwide. To keep the genome of Szabadkígyós having high (-)-a-bisabolol content, Szőke and research team used biotechnological methods. Sterile plantlets, were infected by Agrobacterium rhizogenes strains #A-4, #15834, #R-1601. The hairy root clones possessing the best growing and biosynthetical potential were multiplied for phytochemical investigations. Pharmacologically important compounds of their essential oils were followed in great detail. The amount of in vitro cultured terpenoids and polyin compounds was compared with that of in vivo plants. GC-MS studies showed that sterile chamomile cultures generated the most important terpenoid and polyin compounds characteristics of the mother plant. Berkheyaradulene, geranyl-isovalerat and cedrol as new components were identified in these sterile cultures. The main component of hairy root cultures (D/400, D/1, D/100 and Sz/400) was tr-b-farnesene and in addition one new compound: a-selinene was identified. Hairy root culture originated from chamomile collected in Szabadkígyós was intensive increased the essential oil content and pharmacological active compounds: (-) -α-bisabolol and β-eudesmol was also synthetized in large quantity. Furthermore, in vitro organized cultures were made from this population to obtain propagation material containing numerous active substances.

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RESOURCE-SAVING TECHNOLOGY FOR PROCESSING MEDICINAL SAGE

Известия вузов. Пищевая технология ◽

10.26297/0579-3009.2020.4.12 ◽

2020 ◽

Author(s):

Т.В. ПЕЛИПЕНКО

Keyword(s):

Essential Oil ◽

Raw Materials ◽

Total Yield ◽

Dry Mass ◽

Biologically Active ◽

Flow Diagram ◽

Resource Saving ◽

Process Flow Diagram ◽

Full Flowering ◽

Active Substances

Исследованы потенциальные возможности ресурсосберегающей технологии переработки шалфея лекарственного (ШЛ). Объектом исследования были образцы эфирномасличного сырья – свежеубранные надземные части ШЛ в фазе полного цветения растения и сухие листья ШЛ. Предложенная схема переработки включает следующие стадии: получение эфирного масла; извлечение конкрета из обезэфиренных отходов экстракцией углеводородным растворителем и последующей его отгонкой; получение абсолютного масла и очищенных восков из конкрета отходов; извлечение дитерпеновых соединений экстракцией этиловым спиртом из отходов сырья после обработки гексаном. В результате исследований фракционного состава сырья и особенностей локализации эфирного масла установлено, что массовая доля эфирного масла составляет, % к сухой массе исследуемых фракций: в листьях и соцветиях 2,75, в стеблях 0,31. Рекомендовано введение ограничительных норм по соотношению технически ценной фракции – листьев и соцветий и балластной – стеблей. Полученное из ШЛ эфирное масло имеет высокое содержание туйонов (31,50%), камфоры (21,0%) и 1,8-цинеола (18,80%). Выход дитерпеновых соединений при комплексной переработке эфирномасличного сырья ШЛ на 38,0% ниже, чем из сухих листьев растения. Однако при расчете на сухую массу листьев и соцветий, составляющих 62,3% от массы сырья, показатели отличаются незначительно. Общий выход продуктов, содержащих комплекс биологически активных веществ, составил 15,53 %, что подтверждает целесообразность использования комплексной переработки шалфея лекарственного. The potential of resource-saving technology for processing medicinal sage (MS) has been studied. Samples of essential oil raw materials-freshly harvested aboveground parts of MS in the phase of full flowering of the plant and dry leaves of MS were the object of research. The proposed process flow diagram includes the following stages: obtaining essential oil; recovering a particular from deester waste by extraction with a hydrocarbon solvent and its subsequent distillation; obtaining absolute oil and refined waxes from a specific waste; extraction of diterpene compounds from raw waste after treatment with hexane by extraction with ethyl alcohol. As a result of studies of the fractional composition of raw materials and the features of localization of essential oil, it was found that the mass fraction of essential oil is, % of the dry mass of the studied fractions: 2,75 in leaves and inflorescences, and 0,31 in stems. The introduction of restrictive norms on the ratio of technically valuable fraction – leaves and inflorescences and ballast – stems is recommended. The essential oil obtained from MS has a high content of tuyons (31,50%), camphor (21,0%) and 1,8-cineol (18,80%). The yield of diterpene compounds in the complex processing of essential oil raw materials MS is 38,0% lower than from dry leaves of the plant. However when calculating the dry mass of leaves and inflorescences, which make up 62,3% of the mass of raw materials, the indicators differ slightly. The total yield products containing a complex of biologically active substances amounted to 15,53%, which confirms the feasibility of complex processing of medicinal sage.

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Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study

Molecules ◽

10.3390/molecules23123266 ◽

2018 ◽

Vol 23 (12) ◽

pp. 3266 ◽

Cited By ~ 38

Author(s):

Gokhan Zengin ◽

Luigi Menghini ◽

Antonella Di Sotto ◽

Romina Mancinelli ◽

Francesca Sisto ◽

...

Keyword(s):

Essential Oil ◽

Galleria Mellonella ◽

Cannabis Sativa ◽

Biological Activities ◽

Methodological Approach ◽

Volatile Component ◽

Biologically Active ◽

Antioxidant Compounds

Due to renewed interest in the cultivation and production of Italian Cannabis sativa L., we proposed a multi-methodological approach to explore chemically and biologically both the essential oil and the aromatic water of this plant. We reported the chemical composition in terms of cannabinoid content, volatile component, phenolic and flavonoid pattern, and color characteristics. Then, we demonstrated the ethnopharmacological relevance of this plant cultivated in Italy as a source of antioxidant compounds toward a large panel of enzymes (pancreatic lipase, α-amylase, α-glucosidase, and cholinesterases) and selected clinically relevant, multidrug-sensible, and multidrug-resistant microbial strains (Staphylococcus aureus, Helicobacter pylori, Candida, and Malassezia spp.), evaluating the cytotoxic effects against normal and malignant cell lines. Preliminary in vivo cytotoxicity was also performed on Galleria mellonella larvae. The results corroborate the use of this natural product as a rich source of important biologically active molecules with particular emphasis on the role exerted by naringenin, one of the most important secondary metabolites.

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Ascaridole-rich essential oil from marsh rosemary (Ledum palustre) growing in Poland exerts insecticidal activity on mosquitoes, moths and flies without serious effects on non-target organisms and human cells

Food and Chemical Toxicology ◽

10.1016/j.fct.2020.111184 ◽

2020 ◽

Vol 138 ◽

pp. 111184 ◽

Cited By ~ 3

Author(s):

Giovanni Benelli ◽

Roman Pavela ◽

Kevin Cianfaglione ◽

Joanna Sender ◽

Urban Danuta ◽

...

Keyword(s):

Essential Oil ◽

Insecticidal Activity ◽

Human Cells ◽

Ledum Palustre

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Composition of Essential Oil of Aerial Parts of Chamomilla suaveolens from Estonia

Natural Product Communications ◽

10.1177/1934578x1000500131 ◽

2010 ◽

Vol 5 (1) ◽

pp. 1934578X1000500 ◽

Cited By ~ 2

Author(s):

Anne Orav ◽

Janne Sepp ◽

Tiiu Kailas ◽

Mati Müürisepp ◽

Elmar Arak ◽

...

Keyword(s):

Essential Oil ◽

Palmitic Acid ◽

Essential Oil Composition ◽

Biologically Active Compounds ◽

Biologically Active ◽

Oil Composition ◽

Active Compounds ◽

Aerial Parts ◽

Total Oil

Variations in the essential oil composition of aerial parts of pineapple weed (Chamomilla suaveolens (Pursh) Rydb.) growing wild in Estonia, were determined using GC/FID and GC/MS. Forty-four components were identified, representing over 90% of the total oil. Nine compounds have not been mentioned in the literature before. The principal biologically active compounds in C. suaveolens oils were (Z)-en-yne-dicycloether (17.0 – 40.7%), (E)-β-farnesene (19.5– 32.2%), geranyl isovaleriate (8.4 –18.4%), palmitic acid (0.3 – 9.4%) and myrcene (1.1 – 7.9%). The investigation seems to approve the benefit of using aerial parts of pineapple weed as the substitute for flowers.

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