Integrated Safety Strategy for the Development of Children’s Cosmetic Products Using In Vitro and Clinical Methodologies

2017 ◽  
pp. 565-577
Author(s):  
Andrezza di Pietro Micali Canavez ◽  
Talita M. T. P. Silveira ◽  
Natalia de Albuquerque Vita ◽  
Ana Cristina Weihermann ◽  
Caroline Radoski Neumann ◽  
...  
Keyword(s):  
Cosmetic Products ◽  
Safety Strategy

A New In Vitro Model for Predicting the Toxicity of Cosmetic Products

1992 ◽  
Vol 20 (1) ◽  
pp. 138-143
Author(s):  
Maria Carrara ◽  
Lorenzo Cima ◽  
Roberto Cerini ◽  
Maurizio Dalle Carbonare
Keyword(s):  
Cell Culture ◽  
Cultured Cells ◽  
Neutral Red ◽  
Total Protein Content ◽  
Cosmetic Products ◽  
Cell Culture Insert ◽  
A Cell ◽  
Vitro Model ◽  
Cosmetic Emulsions

A method has been developed whereby cosmetic products which are not soluble in water or in alcohol can be brought into contact with cell cultures by being placed in a cell culture insert, which is then placed in the cell culture well. Preliminary experiments were carried out with L929 cells, and cytotoxicity was evaluated by measuring neutral red uptake and the total protein content of treated cultured cells. Encouraging results were obtained in comparisons of three cosmetic emulsions and of one emulsion containing a range of concentrations of two preservatives, Kathon CG and Bronopol.

scholarly journals Clinical and in vitro evaluation of new anti‐redness cosmetic products in subjects with winter xerosis and sensitive skin

2019 ◽  
Vol 41 (6) ◽  
pp. 534-547 ◽  
Author(s):  
S. J. Nisbet ◽  
D. Targett ◽  
A. V. Rawlings ◽  
K. Qian ◽  
X. Wang ◽  
...  
Keyword(s):  
Cosmetic Products ◽  
Sensitive Skin ◽  
In Vitro Evaluation

scholarly journals Contact Allergy to Fragrances: In Vitro Opportunities for Safety Assessment

Cosmetics ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 3 ◽  
Author(s):  
Emanuela Corsini ◽  
Valentina Galbiati
Keyword(s):  
Safety Assessment ◽  
Contact Allergy ◽  
Hazard Identification ◽  
Quantitative Risk Assessment ◽  
Cosmetic Products ◽  
Animal Testing ◽  
Cosmetic Ingredients ◽  
Substantial Progress ◽  
Allergic Contact

The majority of cosmetic products contain fragrances to make products more pleasant to the consumer, as we all like goods that smell nice. Unfortunately, contact allergy to fragrance compounds is among the most frequent findings in patients with suspected allergic contact dermatitis. In order to revert this and to reduce contact allergy to cosmetics, it is imperative to improve safety assessment of cosmetic products for skin sensitization. In the era of animal ban for cosmetic ingredients, this represents a challenge. Luckily, in the last decades, substantial progress has been made in the understanding of the mechanism of chemical-induced contact allergy and several in vitro methods are available for hazard identification. The purpose of this manuscript is to explore the possibility of non-animal testing for quantitative risk assessment of fragrance-induced contact allergy, essential for cosmetic products, which cannot be tested on animals.

scholarly journals Usefulness of Artificial Membrane, Strat-M®, in the Assessment of Drug Permeation from Complex Vehicles in Finite Dose Conditions

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 173 ◽  
Author(s):  
Florencio Jr Arce ◽  
Narumi Asano ◽  
Gerard Lee See ◽  
Shoko Itakura ◽  
Hiroaki Todo ◽  
...  
Keyword(s):  
Human Skin ◽  
Cosmetic Products ◽  
Artificial Membrane ◽  
Porcine Skin ◽  
Comparative Performance ◽  
Permeability Characteristics ◽  
In Vitro Methods ◽  
Rank Orders ◽  
Franz Diffusion Cells

The ban on the use of animals in testing cosmetic products has led to the development of animal-free in vitro methods. Strat-M® is an artificial membrane engineered to mimic human skin and is recommended as a replacement for skin. However, its usefulness in the assessment of the permeation of cosmetics in in-use conditions remains unverified. No data have been published on its comparative performance with the membrane of choice, porcine skin. The comparative permeability characteristics of Strat-M® and porcine skin were investigated using Franz diffusion cells. Caffeine (CF) and rhododendrol (RD) in complex vehicles with varying concentrations of polyols were applied as finite and infinite doses. Good rank orders of permeation from finite dose experiments were observed for RD. High correlations were observed in RD permeation between Strat-M® and porcine skin under finite and infinite dose conditions, whereas only finite dose conditions for CF were associated with good correlations. Permeation from formulations with high polyol content and residual formulations was enhanced due to the disruption of the integrity of the Strat-M® barrier. The usefulness of Strat-M® in the assessment of dermal permeation may be limited to finite dose conditions and not applicable to infinite dose conditions or formulations applied in layers.

5 Final Report on the Safety Assessment of Propylene Glycol Stearate and Propylene Glycol Stearate Self-Emulsifying

1983 ◽  
Vol 2 (5) ◽  
pp. 101-124 ◽  
Keyword(s):  
Propylene Glycol ◽  
Safety Assessment ◽  
Animal Studies ◽  
Skin Irritation ◽  
Final Report ◽  
Cosmetic Products ◽  
Dermal Toxicity ◽  
Cosmetic Ingredients ◽  
Available Information

Propylene Glycol Stearates (PGS) are a mixture of the mono- and diesters of triple-pressed stearic acid and propylene glycol and are used in a wide variety of cosmetic products. Studies with 14C-labeled PGS show that it is readily metabolized following ingestion. In rats, the acute oral LD50 has been shown to be approximately 25.8 g/kg. The raw ingredient produced no significant dermal toxicity, skin irritation, or eye irritation in acute tests with rabbits. Subchronic animal studies produced no evidence of oral or dermal toxicity. Propylene glycol monostea-rate was negative in in vitro microbial assays for mutagenicity. In clinical studies, PGS produced no significant skin irritation at concentrations up to 55% nor skin sensitization on formulations containing 2.5%. Photo-contact allergenicity tests on product formulations containing 1.5% PGS were negative. From the available information, it is concluded that Propylene Glycol Stearates are safe as cosmetic ingredients in the present practices of use.

A new in vitro approach to verify the moisturizing activity of topical cosmetic products

2018 ◽  
Vol 295 ◽  
pp. S80
Author(s):  
E. Regola ◽  
R. Vicini ◽  
A. Buzzella ◽  
F. Riva ◽  
M. Mori ◽  
...  
Keyword(s):  
Cosmetic Products

The use of in vitro methods in the ocular irritation assessment of cosmetic products

1994 ◽  
Vol 8 (4) ◽  
pp. 893-905 ◽  
Author(s):  
A. Rougier ◽  
M. Cottin ◽  
O. de Silva ◽  
P. Catroux ◽  
R. Roguet ◽  
...  
Keyword(s):  
Cosmetic Products ◽  
In Vitro Methods ◽  
Ocular Irritation

scholarly journals New Dihydroxytyrosyl Esters from Dicarboxylic Acids: Synthesis and Evaluation of the Antioxidant Activity In Vitro (ABTS) and in Cell-Cultures (DCF Assay)

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3135
Author(s):  
Elia Roma ◽  
Elena Mattoni ◽  
Paolo Lupattelli ◽  
Seyed Sepehr Moeini ◽  
Tecla Gasperi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Dicarboxylic Acids ◽  
Antioxidant Effect ◽  
Cosmetic Products ◽  
1H And 13C Nmr ◽  
New Class ◽  
Ft Ir ◽  
New Compounds ◽  
Very High

New dihydroxytyrosyl esters 2a, 2c–2j of dicarboxylic acids were synthesized from methyl orthoformate protected hydroxytyrosol 3 and diacyl chlorides. New compounds were characterized (HRMS, FT-IR, 1H- and 13C-NMR), and tested for antioxidant activity both in vitro (ABTS) and on L6 myoblasts and THP1 leukemic monocytes cell culture by DCF assay. According to the ABTS assay, compounds 2a, 2c–2j showed a TEAC value of antioxidant capacity up to twice that of Trolox. Very high or complete ROS protections were obtained in the cell environment where lipophilicity and rigidity of dicarboxylic structure seem to facilitate the antioxidant effect. MTT assay and proliferation test were used for assessment of cell viability. These compounds can be envisaged as a new class of preservatives for food or cosmetic products.

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