Physical and Chemical Stress Factors in Lactic Acid Bacteria

Nowadays, foods with probiotic bacteria are valuable and desired, because of their influence on human gut and health. Currently, in the era of zero waste, the food industry is interested in managing its waste. Therefore, the aim of the study was to determine the influence of drying process on the physicochemical properties of fermented vegetable pomace. The work included examining the influence of the lactic acid bacteria (Levilactobacillus brevis, Lactiplantibacillus plantarum, Limosilactobacillus fermentum and its mixture in the ratio 1:1:1) used for vegetable fermentation (beetroot, red pepper, carrot), obtaining pomace from fermented vegetables, and then selection of drying technique using the following methods: convection drying (CD) or freeze-drying (FD) on the physical and chemical properties of pomace. In the obtained pomace and its dried form, dry substance, water activity, color, and active substances such as betalains and carotenoids by spectrophotometric method and also bacteria concentration were evaluated. After fermentation of pomace from the same vegetable, a similar concentration of lactic acid bacteria was found as well as dry substances, color and colorants. Results of physico-chemical properties were related to the used vegetable type. After drying of pomace, it could be seen a high decrease in bacteria and colorant concentration (betalains, carotenoids) independently from drying and vegetable type as well as used starter cultures. The smallest change was observed for spontaneously fermented vegetables compared to those in which the starter culture was used.

Download Full-text

WILDIERS' BIOS AND THE LACTIC ACID BACTERIA: THE RELATION OF BIOS TO THE WATER-SOLUBLE B-VITAMINS

Canadian Journal of Research ◽

10.1139/cjr38b-005 ◽

1938 ◽

Vol 16b (2) ◽

pp. 46-53 ◽

Cited By ~ 3

Author(s):

Blythe Alfred Eagles ◽

Olga Okulitch ◽

Arthur Stephen Kadzielawa

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Chemical Properties ◽

Water Soluble ◽

Vitamin B ◽

Physical And Chemical Properties ◽

Heat Stable ◽

Physical And Chemical ◽

Growth Of Animals ◽

And Chemical Properties

The influence of three distinct activators prepared from tomatoes, yeast, or liver, on the metabolism of two species of lactic acid bacteria has been studied. One of these activators is Bios II A, and the other two have been shown to be components constituting Bios II B. On the basis of their physical and chemical properties, it is suggested that the growth stimulants required by the lactic acid bacteria are identical with certain of the heat-stable accessory food factors of the Vitamin-B complex essential for the growth of animals.

Download Full-text

Real-time study of the effect of different stress factors on lactic acid bacteria by electrochemical optical waveguide lightmode spectroscopy

Biomolecular Engineering ◽

10.1016/j.bioeng.2007.10.001 ◽

2007 ◽

Vol 24 (6) ◽

pp. 631-637 ◽

Cited By ~ 9

Author(s):

Edina Németh ◽

Nóra Adányi ◽

Anna Halász ◽

Mária Váradi ◽

István Szendrő

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Real Time ◽

Optical Waveguide ◽

Stress Factors ◽

Time Study

Download Full-text

Investigating the effects of different physical and chemical stress factors on microbial biofilm

Water SA ◽

10.4314/wsa.v44i2.16 ◽

2018 ◽

Vol 44 (2 April) ◽

Cited By ~ 2

Author(s):

Cansu Vatansever ◽

Irfan Turetgen

Keyword(s):

Environmental Conditions ◽

Cooling Tower ◽

Solid Phase ◽

Culture Technique ◽

Stress Factors ◽

Chemical Stress ◽

Dapi Staining ◽

Conventional Culture ◽

Physical And Chemical ◽

The Impact

Microorganisms that adhere to surfaces in order to protect themselves from many adverse environmental conditions form a layer called biofilm. Biofilms protect bacteria from changing environmental conditions such as starvation, antibiotics, disinfectants, pH and temperature fluctuation, dryness and UV rays. In this study, biofilms were formed on surfaces of glass coupons in a cooling tower model system over a period of 180 days. The biofilms were treated with various stress factors monthly. These stress factors were: exposure to temperatures of 4°C and 60°C, pH of 3, 5, and 11, 3 M aqueous NaCl and distilled water, as well as, monochloramine at 2, 500, and 1 000 mg/L (ppm). Following the treatment with stress factors, both the numbers of actively respiring bacteria and the total bacteria in the biofilms were determined by CTC-DAPI staining. The aerobic heterotrophic plate counts (HPC) in the biofilms were determined by the conventional culture method of spread plating on R2A agar. The aim of this study was to determine the impact of these stressors on the model cooling tower biofilms. Of the stressors tested, those that had the greatest impact were a temperature of 60°C, pH of 3, 3 M NaCl, and monochloramine at both 500 and 1 000 mg/L. However, when using a non-culture-based viability assay (CTC-DAPI staining), an extremely high number of live bacteria were detected even after applying the most effective stress factors (with the exception of pH 3) of 60°C, 3 M NaCl, monochloramine at 500 and 1 000 mg/L. Results showed that biofilm protects the bacteria from extreme physical and chemical stress conditions. Additionally, the conventional culture technique cannot detect the presence of bacteria that have entered the viable but non-culturable (VBNC) phase; the use of different techniques, such as microscopy and cytometry (flow and solid-phase), is therefore important to obtain more accurate results.

Download Full-text

POPULASI BAKTERI ASAM LAKTAT PADA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI LIMBAH PERTANIAN DENGAN SUPLEMEN ENCENG GONDOK DAN PROBIOTIK

Scripta Biologica ◽

10.20884/1.sb.2014.1.1.32 ◽

2014 ◽

Vol 1 (1) ◽

pp. 87

Author(s):

Andhika Agus Setyawan ◽

Sukanto Sukanto ◽

Endang Widyastuti

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Water Hyacinth ◽

Agricultural Waste ◽

Randomized Design ◽

Fermented Feed ◽

Independent Variable ◽

Completely Randomized Design ◽

Chemical Conditions ◽

Physical And Chemical

The aim of this study were to determine the effect of fermented agricultural wastes feeding with supplement of water hyacinth and MEP+ probiotic towards the increasing population of intestinal Lactid Acid Bacteria (LAB) in Tilapia and to know the highest population of lactic acid bacteria after fermented agricultural waste feeding with water hyacinth supplements and MEP+ probiotic. Independent variable in this study was fermented feed with the addition of different water hyacinth and MEP+ probiotics. The experimental study was continued using completely randomized design (CRD). The dependent variable in this study was the number density of Lactobacillus bacteria in the gut of Tilapia. The main parameters measured were the number of LAB. Proponent parameters were namely Tilapia weight gain, physical and chemical conditions of the water. The results showed that feeding fermentative effected in increasing the population of lactic acid bacteria in the gut of Tilapia. Fermentative feed supplement agricultural waste with water hyacinth as much as 10% and MEP+ probiotic was the best treatment with LAB population density in Tilapia intestine with the average of 5.85 x 106 CFU /g.

Download Full-text