Determination of the biologically active components of the rhizomes of Rhodiola rosea

1991 ◽  
Vol 27 (3) ◽  
pp. 276-279 ◽  
Author(s):  
A. A. Kur'yanov ◽  
L. T. Bondarenko ◽  
V. A. Kurkin ◽  
G. G. Zapesochnaya ◽  
A. A. Dubichev ◽  
...  
Keyword(s):  
Biologically Active ◽  
Rhodiola Rosea ◽  
Active Components

scholarly journals Simultaneous Determination of 78 Compounds of Rhodiola rosea Extract by Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Alexander M. Zakharenko ◽  
Mayya P. Razgonova ◽  
Konstantin S. Pikula ◽  
Kirill S. Golokhvast
Keyword(s):  
Supercritical Co2 ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Supercritical Co2 Extraction ◽  
Active Components ◽  
Glucaric Acid ◽  
Experimental Conditions ◽  
Target Analytes ◽  
Trimethyl Ether

The plant Rhodiola rosea L. of family Crassulaceae was extracted using the supercritical CO2-extraction method. Several experimental conditions were investigated in the pressure range of 200–500 bar, with the used volume of cosolvent ethanol in the amount of 1% in the liquid phase at a temperature in the range of 31–70°C. The most effective extraction conditions are pressure 350 bar and temperature 60°C. The extracts were analyzed by HPLC with MS/MS identification. 78 target analytes were isolated from Rhodiola rosea (Russia) using a series of column chromatography and mass spectrometry experiments. The results of the analysis showed a spectrum of the main active ingredients Rh. rosea: salidroside, rhodiolosides (B and C), rhodiosin, luteolin, catechin, quercetin, quercitrin, herbacetin, sacranoside A, vimalin, and others. In addition to the reported metabolites, 29 metabolites were newly annotated in Rh. rosea. There were flavonols: dihydroquercetin, acacetin, mearnsetin, and taxifolin-O-pentoside; flavones: apigenin-O-hexoside derivative, tricetin trimethyl ether 7-O-hexosyl-hexoside, tricin 7-O-glucoronyl-O-hexoside, tricin O-pentoside, and tricin-O-dihexoside; flavanones: eriodictyol-7-O-glucoside; flavan-3-ols: gallocatechin, hydroxycinnamic acid caffeoylmalic acid, and di-O-caffeoylquinic acid; coumarins: esculetin; esculin: fraxin; and lignans: hinokinin, pinoresinol, L-ascorbic acid, glucaric acid, palmitic acid, and linolenic acid. The results of supercritical CO2-extraction from roots and rhizomes of Rh. rosea, in particular, indicate that the extract contained all biologically active components of the plant, as well as inert mixtures of extracted compositions.

scholarly journals DETERMINATION OF SAFETY PROFILE OF REPARATIVE ACTIVITY OF THE NEW HYGIENIC PRODUCT “APIPROL”

2021 ◽  
Vol 74 (8) ◽  
pp. 1885-1890
Author(s):  
Lyudmila Kravchenko ◽  
Elena Appelkhans ◽  
Liana Unhurian ◽  
Sergey Shcherbakov ◽  
Olga Goncharenko ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Biologically Active ◽  
Single Application ◽  
Regenerative Processes ◽  
Active Components ◽  
White Rats ◽  
Experimental Trauma ◽  
Daily Dose ◽  
Further Development

The aim: To prove safety of the new elixir and determine the effect on regenerative processes under conditions of the experimental trauma. Materials and methods: The formula of the new dental elixir “Apiprol” includes biologically active components of bee products (propolis, comb capping wax), plant origin compounds. The elixir was single administered, intragastrally to white nonlinear mice weighing (22±2) g at doses at 50 mg/kg, 500 mg/kg, 5000 mg/kg. Acute toxicity of the new hygienic product “Apiprol” was investigated by a single application at the rate of 1000 and 5000 mg/kg animal weight. Subacute (chronic) toxicity was studied when elixir was applied to the skin of 10 mice at a single daily dose of 500 mg/kg for one month. A local irritant effect of the elixir on the oral mucosa was examined in 14 white rats daily after application for 3–5 min. The study of reparative activity was carried out on an experimental model of the wound process. Results: The elixir’s components promote tissues regeneration, rapid epithelialization and wound surfaces healing, having a highly immune stimulating activity and antibacterial properties. Conclusions: The performed study proved a complete safety of the new elixir “Apiprol” and indicated a necessity of the further development of this preparation in order to create a greater variety of dental means produced in Ukraine.

scholarly journals Subclinical hypothyroidism: do you always need hormone replacement therapy? A modern view of the problem

2021 ◽  
Vol 17 (5) ◽  
pp. 418-421
Author(s):  
N.O. Kravchun ◽  
I.P. Dunaeva
Keyword(s):  
Trace Elements ◽  
Subclinical Hypothyroidism ◽  
Elderly Women ◽  
Clinical Symptoms ◽  
Hormone Replacement ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Active Components ◽  
Stage Of Development ◽  
Wide Range

Subclinical hypothyroidism is the initial stage of development of hypothyroidism, in which there are no clear clinical symptoms and there is a slow decline in thyroid function. It is known that subclinical hypothyroidism occurs in the population much more often than overt one, its prevalence depends on gender, age of patients and it is much more often diagnosed in elderly women. The literature review considers the effectiveness of the combined herbal preparation to improve the quality of life in hypothyroidism — Endomar, which contains 50 % of Potentilla alba, 25 % of Skutellaria baicalensis and 25 % of Rhodiola rosea. All three components of Endomar are synergistic in hypothyroidism. This multicomponent herbal product has a wide range of therapeutic properties and can be recommended for subclinical forms of thyroid hypofunction. Potentilla alba has been used in medical practice since ancient times. Biologically active components of Potentilla alba extract are flavonoids that regulate the permeability and elasticity of blood vessel walls, thereby neutralizing free radi-cals, phenolcarboxylic acids having antimutagenic and diuretic properties, saponin glycosides, which have a cardiotonic, neurotropic effect, hypocholesterolemic, adaptogenic, sedative action. Rhodiola rosea, an extract of the roots and rhizomes of this plant, is an adaptogen that increases the body’s resistance to stress, exhaustion, fatigue. Rhodiola rosea includes the following active substances: phenols and their derivatives aurol, salidroside; flavonoids: kaempferol, rhodiolin, rhodionin, rhodiosine, trace elements. Endomar includes Skutellaria baicalensis, which has unique properties. This plant contains flavonoid baicalin, which has a neuroprotective and neotropic effect, flavonoids scutellarin and vagonin that maintain normal vascular tone. In general, all components have an antioxidant, antispasmodic, antiinflammatory effect, they are also adaptogens and include trace elements such as magnesium, zinc, selenium.

UPLC-MS/MS determination of chlorogenic acid, hyperoside and astragalin in plasma and its pharmacokinetic application in liver injury rats

2020 ◽  
Vol 16 ◽  
Author(s):  
Ying Zhang ◽  
Shu-ya Xu ◽  
Zhe Jia ◽  
Ting Han ◽  
Meng-nan Liu ◽  
...  
Keyword(s):  
Liver Injury ◽  
Chlorogenic Acid ◽  
Multiple Reaction Monitoring ◽  
Rat Plasma ◽  
Biologically Active ◽  
Hepatoprotective Effect ◽  
Plasma Samples ◽  
Active Components ◽  
Three Components

Background: Cuscutae semen (CS) is reported to show hepatoprotective effect. Chlorogenic acid, hyperoside and astragalin are three major biologically active components from CS. Objective: A sensitive method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed and validated to quantify the three components in rat plasma and was succssfully used to pharmacokinetic study in liver injury rats. Method: Plasma samples were prepared with protein precipitation by acetonitrile. Chromatographic separation was achieved on ACQUITY-XBridge BEH C18 column with a gradient elution using the mobile phase containing 0.05% formic acid in water (A) and acetonitrile (B). The three components were quantified using electrospray ionization (ESI) source in the negative multiple reaction monitoring (MRM) mode. Results and Discussion: Calibration curves of each analyte showed a good linearity with correlation coefficients over 0.99. Accuracies (RE%) and precisions (RSD%) were within 15%. The method was stable. Recovery of the target compounds in plasma samples ranged from 87.00% to 102.29%. No matrix effect was found to influence the quantitative method. Conclusion: The UPLC-MS/MS method was met the acceptance criteria and successfully applied to simultaneous determination of chlorogenic acid, hyperoside and astragalin in rat plasma for the first time. It is suitable for pharmacokinetic application in liver injury rats. It provides the foundation for the further development and utilization for the hepatoprotective effect of cuscutae semen.

scholarly journals Chemical profile and antimicrobial activity of extractable compounds of Betula litwinowii (Betulaceae) buds

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Valery A. Isidorov ◽  
Róża Bagan ◽  
Lech Szczepaniak ◽  
Izabela Swiecicka
Keyword(s):  
Antimicrobial Activity ◽  
Folk Medicine ◽  
Biologically Active ◽  
Chemical Profile ◽  
Active Components ◽  
Volatile Elements ◽  
Gram Positive Bacteria ◽  
History Of ◽  
Extractable Compounds

AbstractIn spite of the long history of therapeutic use of buds from different birch species in folk medicine the existing information on their chemical composition is insufficient. The main goal was to develop a method for GC-MS determination of the chemical profile of birch buds as well as their antimicrobial activity. 150 substances of different classes were identified in Betula litwinowii buds. The volatile elements of the buds were mainly represented by sesquiterpene compounds. Ether extracts also contained other biologically active components such as flavonoids and triterpenoids. However, a particular feature of this fraction was the high content of sesquiterpene phenylpropenoids, including esters of ferulic and caffeic acids with caryophyllene-type alcohols that had not been previously found in any biological samples. Apart from carbohydrates, a series of free amino acids were detected in methanol extracts. The antimicrobial activity of the ether extracts of the buds was observed against all of the microorganisms tested, with MIC values from 0.04 to 0.08 mg mL-1 for Gram-positive bacteria and Candida albicans. However, their inhibitory activities against tested Gram-negative bacteria were rather occasional.

scholarly journals STANDARDIZATION PROBLEMS OF MEDICINAL PREPARATIONS FROM RHODIOLA ROSEA L.

2021 ◽  
Vol 9 (3) ◽  
pp. 185-194
Author(s):  
V. A. Kurkin ◽  
T. K. Ryazanova
Keyword(s):  
Raw Materials ◽  
Statistical Processing ◽  
Reversed Phase ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Plant Materials ◽  
Liquid Extract ◽  
Medicinal Preparations ◽  
Methodological Approaches

Rhodiola rosea L. rhizomes and roots are pharmacopoeial raw materials, which are used in official medicine for obtaining medicines with adaptogenic activity. One of the most common problems in the production of medicines from Rhodiola rosea L. rhizomes and roots is the use of poor quality medicinal plant materials, which leads to the absence of biologically significant compounds in the preparations. One of the possible reasons is the shortcomings in the existing approaches to the standardization of Rhodiola rosea L. raw materials and preparations.The aim of the study is the improvement of approaches to the standardization of medicinal preparations from Rhodiola rosea L. rhizomes and roots.Materials and methods. Experimental and industrial samples of liquid extract from Rhodiola rosea L. roots, as well as reference samples of rosavin and salidroside, were used as materials of the research. The HPLC analysis was carried out using a Milichrom-6 chromatograph (NPAO Nauchpribor) under the following conditions of reversed-phase chromatography in an isocratic mode: a steel column KAKH-6-80-4 (2 mm x 80 mm; Separon-C18 7 μm), a mobile phase – acetonitrile: 1% solution of acetic acid in water in the ratio of 14:86, the elution rate was 100 μL/min, the eluent volume was 2000 μL. The constituents were detected at the wavelength of 252 nm (rosavin) and 278 nm (salidroside).Results. An assay of rosavin and salidroside in the liquid extract of Rhodiola rosea L. was developed using the HPLC method. It was determined that the content of rosavin in the samples of the liquid extracts obtained from Rhodiola rosea L. rhizomes and roots of the pharmacopoeial quality, varied from 0.21%±0.03% to 0.32%±0.04%, salidroside – from 1.13% ±0.05% to 2.71%±0.12%, respectively. The results of statistical processing indicate that the relative error of the average result for the determination of rosavin and salidroside in the preparations of Rhodiola rosea L. with a confidence level of 95% does not exceed ±6.0%.Conclusion. Thus, methodological approaches to the analysis of medicinal preparations from Rhodiola rosea L. rhizomes and roots have been substantiated. These methodological approaches consist of the quantitative determination of the dominant and diagnostically significant biologically active compounds – rosavin and salidroside.

Extraction and determination of biologically active components of St. John’s wort and its pharmaceutical preparations

2016 ◽  
Vol 71 (7) ◽  
pp. 741-747 ◽  
Author(s):  
V. V. Milevskaya ◽  
M. A. Statkus ◽  
Z. A. Temerdashev ◽  
N. V. Kiseleva ◽  
T. S. Butyl’skaya ◽  
...  
Keyword(s):  
Pharmaceutical Preparations ◽  
Biologically Active ◽  
Active Components ◽  
St John’S Wort ◽  
St John's Wort

scholarly journals Phenanthrenes from Juncus Compressus Jacq. with Promising Antiproliferative and Anti-HSV-2 Activities

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2085 ◽  
Author(s):  
Csaba Bús ◽  
Norbert Kúsz ◽  
Gusztáv Jakab ◽  
Seyyed Senobar Tahaei ◽  
István Zupkó ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
2D Nmr ◽  
Biologically Active ◽  
Active Components ◽  
In Vitro Investigation ◽  
First Time

Juncaceae species are rich sources of phenanthrenes. The present study has focused on the isolation and structure determination of biologically active components from Juncus compressus. Eleven compounds (nine phenanthrenes and two flavonoids) have been isolated from the plant by the combination of different chromatographic methods. Two compounds (compressins A (Compound 1) and B (Compound 2)) are novel natural products, while seven phenanthrenes (effusol (Compound 3), effususol (Compound 4), juncusol (Compound 5), 2-hydroxy-1-methyl-4-oxymethylene-5-vinyl-9,10-dihydrophenanthrene (Compound 6), 7-hydroxy-1-methyl-2-methoxy-5-vinyl-9,10-dihydrophenanthrene (Compound 7), effususin A (Compound 8), and dehydroeffusol (Compound 9)), and two flavonoids (apigenin (Compound 10) and luteolin (Compound 11) were isolated for the first time from the plant. Compressin B (Compound 2) is a dimeric phenanthrene, in which two juncusol monomers (Compound 5) are connecting through their C-3 atoms. The structure elucidation of the isolated compounds was carried out using 1D, 2D NMR spectroscopic methods and HR-MS measurements. In vitro investigation of the antiproliferative effect of the phenanthrenes on two cervical (HeLa and SiHa) and an ovarian human tumor cell line (A2780) revealed that compounds have remarkable antiproliferative activity, mainly on the HeLa cell line. Moreover, juncusol (Compound 5) proved to possess significant antiviral activity against the herpes simplex 2 virus (HSV-2).

PRODUCTION OF BLENDED JUICE FROM PUMPKIN, QUINCE, PERSIMMON

2018 ◽  
pp. 59-62
Author(s):  
I. A. Kyazimova ◽  
А. А. Kasumova ◽  
А. А. Nabiev
Keyword(s):  
Phenolic Compounds ◽  
Organic Acids ◽  
New Technology ◽  
Mineral Elements ◽  
Biologically Active ◽  
Active Components ◽  
Aliphatic Acids ◽  
Free Glucose ◽  
Dietary Value ◽  
Quantitative Indicators

Production of plant products, including juices around the world increases continuously. In the fruit and vegetable juices contain a significant amount of monosaccharides (glucose and fructose), organic acids, vitamins, phenolic compounds, mineral substances and other biologically active components that determine the nutritional and dietary value. For the prevention of various diseases associated with impaired metabolic processes, we developed a new technology of preparation of food by blending juice of pumpkin, quince and persimmon. Thus prepared organic blended juice contains a substantial amount of free glucose and fructose, different phenolic compounds, a sufficient amount of organic acids, mineral elements, including iodine and other components that determine its nutritional and biological value. In prepared juices were evaluated the quantitative indicators of β-carotene, vitamin C, glucose and fructose, sucrose, starch, pectin substances. Also in the atomic absorbtion spectrometer Analyst 400 (PerkinElmer, USA) was analyzed content of the organic acids and phenolic compounds. Prepared juices were tested in accordance with 10 point scoring scale. It is established that all juices contain a sufficient amount of the minerals. In pumpkin and quince juices not contain iodine while it presents in sufficient amount in persimmon juice that’s why in the blended juice mineral in addition to mineral elements iodine are contained. In pumpkin and persimmon aliphatic acids are contained in small amount. For this reason during the blending process was used quince juice which is rich in aliphatic acids. The blended juice is light straw color, with delicious flavor, a slight astringent property and a balanced taste.

Fast Method for the Determination of Residual Solvents in Radiopharmaceutical Products

2017 ◽  
Vol 68 (4) ◽  
pp. 666-670 ◽  
Author(s):  
Mirela Mihon ◽  
Catalin Stelian Tuta ◽  
Alina Catrinel Ion ◽  
Dana Niculae ◽  
Vasile Lavric
Keyword(s):  
Gas Chromatography ◽  
Control Method ◽  
Limit Of Detection ◽  
The Body ◽  
Biologically Active ◽  
Injection Technique ◽  
Fast Method ◽  
Residual Solvent ◽  
Residual Solvents

The aim of this work was the development and validation of a fast analytical method to determine the residual solvents content in radiopharmaceuticals such as: 18F-Fluorodeoxyglucose (18F-FDG), 18F-Fluoroestradiol (18F-FES), 18F-Fluorothymidine (18F-FLT),18F-Fluoromisonidazole (18F-FMISO). Radiopharmaceuticals are radioactive preparations for medical purposes used in nuclear medicine as tracers in diagnostic imaging and treatment of certain diseases. Positron Emission Tomography (PET) is a medical imaging technique that consists in introducing into the body of a small amount of a biologically active chemical compound labelled with a short lived positron-emitting radioisotope (18F, 11C, 68Ga). Residual solvents are critical impurities in radiopharmaceuticals that can affect labelling, stability and physicochemical properties of drugs. Therefore, the determination of these solvents is essential for quality control of radiopharmaceuticals. Validation of the control method for residual solvents by gas chromatography is referred by the European Pharmacopoeia using a special injection technique (head space). The parameters of the method, which comply with International Conference on Harmonization guidelines, are: accuracy, precision, linearity, limit of detection, limit of quantification and robustness. The proposed method (direct gas chromatography injection) proved to be linear, precise, accurate and robust. Good linearity was achieved for all the solvents and correlation coefficients (R2) for each residual solvent were found more than 0.99.

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