scholarly journals DETERMINATION OF SAFETY PROFILE OF REPARATIVE ACTIVITY OF THE NEW HYGIENIC PRODUCT “APIPROL”

2021 ◽  
Vol 74 (8) ◽  
pp. 1885-1890
Author(s):  
Lyudmila Kravchenko ◽  
Elena Appelkhans ◽  
Liana Unhurian ◽  
Sergey Shcherbakov ◽  
Olga Goncharenko ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Biologically Active ◽  
Single Application ◽  
Regenerative Processes ◽  
Active Components ◽  
White Rats ◽  
Experimental Trauma ◽  
Daily Dose ◽  
Further Development

The aim: To prove safety of the new elixir and determine the effect on regenerative processes under conditions of the experimental trauma. Materials and methods: The formula of the new dental elixir “Apiprol” includes biologically active components of bee products (propolis, comb capping wax), plant origin compounds. The elixir was single administered, intragastrally to white nonlinear mice weighing (22±2) g at doses at 50 mg/kg, 500 mg/kg, 5000 mg/kg. Acute toxicity of the new hygienic product “Apiprol” was investigated by a single application at the rate of 1000 and 5000 mg/kg animal weight. Subacute (chronic) toxicity was studied when elixir was applied to the skin of 10 mice at a single daily dose of 500 mg/kg for one month. A local irritant effect of the elixir on the oral mucosa was examined in 14 white rats daily after application for 3–5 min. The study of reparative activity was carried out on an experimental model of the wound process. Results: The elixir’s components promote tissues regeneration, rapid epithelialization and wound surfaces healing, having a highly immune stimulating activity and antibacterial properties. Conclusions: The performed study proved a complete safety of the new elixir “Apiprol” and indicated a necessity of the further development of this preparation in order to create a greater variety of dental means produced in Ukraine.

scholarly journals STUDY OF TOXICOLOGICAL PROPERTIES AND LOCAL IRRITANT EFFECT OF THE DRUGS INSACAR TOTAL C PLUS AND INSACAR TOTAL K PLUS ON LABORATORY RATS

2021 ◽  
pp. 330-335
Author(s):  
Makhvatova
Keyword(s):  
Parasite Species ◽  
Systemic Effect ◽  
Single Application ◽  
Active Components ◽  
Cutaneous Toxicity ◽  
Laboratory Rats ◽  
Entire Observation Period ◽  
White Rats ◽  
Active Substances ◽  
Observation Period

The study of acute cutaneous toxicity and local irritant effect of the drugs Insacar Total C Plus and Insacar Total K Plus on mongrel white rats was conducted in doses of 5000 and 10 000 mg/kg of body weight (BW). When the drugs were applied to the skin of rats, no animal deaths were recorded during the entire observation period. Thus, the LD50 is more than 10 000 mg / kg of BW. A single application of the drugs to the skin in rats at a dose of 1 ml/100 g (10 000 units) in two experimental groups on the 7th and 9th day of the study showed the most pronounced irritant effect. In this regard, the drugs have an irritating effect – 1 point. The studied drugs Insacar Total C Plus and Insacar Total K Plus belong to the group of insectoacaricidal and anthelmintic agents. The substances included in its composition have a contact and systemic effect on ectoparasites and nematodes. They are complex medicines containing 4 active components – moxidectin, pyriproxifen, imidacloprid, praziquantel. Such a combination of active substances is characterized by effectiveness against a larger number of parasite species, stages of their development, and in addition, reduce the likelihood of developing resistance of pathogens to drugs.

UPLC-MS/MS determination of chlorogenic acid, hyperoside and astragalin in plasma and its pharmacokinetic application in liver injury rats

2020 ◽  
Vol 16 ◽  
Author(s):  
Ying Zhang ◽  
Shu-ya Xu ◽  
Zhe Jia ◽  
Ting Han ◽  
Meng-nan Liu ◽  
...  
Keyword(s):  
Liver Injury ◽  
Chlorogenic Acid ◽  
Multiple Reaction Monitoring ◽  
Rat Plasma ◽  
Biologically Active ◽  
Hepatoprotective Effect ◽  
Plasma Samples ◽  
Active Components ◽  
Three Components

Background: Cuscutae semen (CS) is reported to show hepatoprotective effect. Chlorogenic acid, hyperoside and astragalin are three major biologically active components from CS. Objective: A sensitive method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed and validated to quantify the three components in rat plasma and was succssfully used to pharmacokinetic study in liver injury rats. Method: Plasma samples were prepared with protein precipitation by acetonitrile. Chromatographic separation was achieved on ACQUITY-XBridge BEH C18 column with a gradient elution using the mobile phase containing 0.05% formic acid in water (A) and acetonitrile (B). The three components were quantified using electrospray ionization (ESI) source in the negative multiple reaction monitoring (MRM) mode. Results and Discussion: Calibration curves of each analyte showed a good linearity with correlation coefficients over 0.99. Accuracies (RE%) and precisions (RSD%) were within 15%. The method was stable. Recovery of the target compounds in plasma samples ranged from 87.00% to 102.29%. No matrix effect was found to influence the quantitative method. Conclusion: The UPLC-MS/MS method was met the acceptance criteria and successfully applied to simultaneous determination of chlorogenic acid, hyperoside and astragalin in rat plasma for the first time. It is suitable for pharmacokinetic application in liver injury rats. It provides the foundation for the further development and utilization for the hepatoprotective effect of cuscutae semen.

scholarly journals Chemical profile and antimicrobial activity of extractable compounds of Betula litwinowii (Betulaceae) buds

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Valery A. Isidorov ◽  
Róża Bagan ◽  
Lech Szczepaniak ◽  
Izabela Swiecicka
Keyword(s):  
Antimicrobial Activity ◽  
Folk Medicine ◽  
Biologically Active ◽  
Chemical Profile ◽  
Active Components ◽  
Volatile Elements ◽  
Gram Positive Bacteria ◽  
History Of ◽  
Extractable Compounds

AbstractIn spite of the long history of therapeutic use of buds from different birch species in folk medicine the existing information on their chemical composition is insufficient. The main goal was to develop a method for GC-MS determination of the chemical profile of birch buds as well as their antimicrobial activity. 150 substances of different classes were identified in Betula litwinowii buds. The volatile elements of the buds were mainly represented by sesquiterpene compounds. Ether extracts also contained other biologically active components such as flavonoids and triterpenoids. However, a particular feature of this fraction was the high content of sesquiterpene phenylpropenoids, including esters of ferulic and caffeic acids with caryophyllene-type alcohols that had not been previously found in any biological samples. Apart from carbohydrates, a series of free amino acids were detected in methanol extracts. The antimicrobial activity of the ether extracts of the buds was observed against all of the microorganisms tested, with MIC values from 0.04 to 0.08 mg mL-1 for Gram-positive bacteria and Candida albicans. However, their inhibitory activities against tested Gram-negative bacteria were rather occasional.

Determination of the biologically active components of the rhizomes of Rhodiola rosea

1991 ◽  
Vol 27 (3) ◽  
pp. 276-279 ◽  
Author(s):  
A. A. Kur'yanov ◽  
L. T. Bondarenko ◽  
V. A. Kurkin ◽  
G. G. Zapesochnaya ◽  
A. A. Dubichev ◽  
...  
Keyword(s):  
Biologically Active ◽  
Rhodiola Rosea ◽  
Active Components

Extraction and determination of biologically active components of St. John’s wort and its pharmaceutical preparations

2016 ◽  
Vol 71 (7) ◽  
pp. 741-747 ◽  
Author(s):  
V. V. Milevskaya ◽  
M. A. Statkus ◽  
Z. A. Temerdashev ◽  
N. V. Kiseleva ◽  
T. S. Butyl’skaya ◽  
...  
Keyword(s):  
Pharmaceutical Preparations ◽  
Biologically Active ◽  
Active Components ◽  
St John’S Wort ◽  
St John's Wort

scholarly journals Simultaneous Determination of 78 Compounds of Rhodiola rosea Extract by Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Alexander M. Zakharenko ◽  
Mayya P. Razgonova ◽  
Konstantin S. Pikula ◽  
Kirill S. Golokhvast
Keyword(s):  
Supercritical Co2 ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Supercritical Co2 Extraction ◽  
Active Components ◽  
Glucaric Acid ◽  
Experimental Conditions ◽  
Target Analytes ◽  
Trimethyl Ether

The plant Rhodiola rosea L. of family Crassulaceae was extracted using the supercritical CO2-extraction method. Several experimental conditions were investigated in the pressure range of 200–500 bar, with the used volume of cosolvent ethanol in the amount of 1% in the liquid phase at a temperature in the range of 31–70°C. The most effective extraction conditions are pressure 350 bar and temperature 60°C. The extracts were analyzed by HPLC with MS/MS identification. 78 target analytes were isolated from Rhodiola rosea (Russia) using a series of column chromatography and mass spectrometry experiments. The results of the analysis showed a spectrum of the main active ingredients Rh. rosea: salidroside, rhodiolosides (B and C), rhodiosin, luteolin, catechin, quercetin, quercitrin, herbacetin, sacranoside A, vimalin, and others. In addition to the reported metabolites, 29 metabolites were newly annotated in Rh. rosea. There were flavonols: dihydroquercetin, acacetin, mearnsetin, and taxifolin-O-pentoside; flavones: apigenin-O-hexoside derivative, tricetin trimethyl ether 7-O-hexosyl-hexoside, tricin 7-O-glucoronyl-O-hexoside, tricin O-pentoside, and tricin-O-dihexoside; flavanones: eriodictyol-7-O-glucoside; flavan-3-ols: gallocatechin, hydroxycinnamic acid caffeoylmalic acid, and di-O-caffeoylquinic acid; coumarins: esculetin; esculin: fraxin; and lignans: hinokinin, pinoresinol, L-ascorbic acid, glucaric acid, palmitic acid, and linolenic acid. The results of supercritical CO2-extraction from roots and rhizomes of Rh. rosea, in particular, indicate that the extract contained all biologically active components of the plant, as well as inert mixtures of extracted compositions.

scholarly journals Phenanthrenes from Juncus Compressus Jacq. with Promising Antiproliferative and Anti-HSV-2 Activities

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2085 ◽  
Author(s):  
Csaba Bús ◽  
Norbert Kúsz ◽  
Gusztáv Jakab ◽  
Seyyed Senobar Tahaei ◽  
István Zupkó ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
2D Nmr ◽  
Biologically Active ◽  
Active Components ◽  
In Vitro Investigation ◽  
First Time

Juncaceae species are rich sources of phenanthrenes. The present study has focused on the isolation and structure determination of biologically active components from Juncus compressus. Eleven compounds (nine phenanthrenes and two flavonoids) have been isolated from the plant by the combination of different chromatographic methods. Two compounds (compressins A (Compound 1) and B (Compound 2)) are novel natural products, while seven phenanthrenes (effusol (Compound 3), effususol (Compound 4), juncusol (Compound 5), 2-hydroxy-1-methyl-4-oxymethylene-5-vinyl-9,10-dihydrophenanthrene (Compound 6), 7-hydroxy-1-methyl-2-methoxy-5-vinyl-9,10-dihydrophenanthrene (Compound 7), effususin A (Compound 8), and dehydroeffusol (Compound 9)), and two flavonoids (apigenin (Compound 10) and luteolin (Compound 11) were isolated for the first time from the plant. Compressin B (Compound 2) is a dimeric phenanthrene, in which two juncusol monomers (Compound 5) are connecting through their C-3 atoms. The structure elucidation of the isolated compounds was carried out using 1D, 2D NMR spectroscopic methods and HR-MS measurements. In vitro investigation of the antiproliferative effect of the phenanthrenes on two cervical (HeLa and SiHa) and an ovarian human tumor cell line (A2780) revealed that compounds have remarkable antiproliferative activity, mainly on the HeLa cell line. Moreover, juncusol (Compound 5) proved to possess significant antiviral activity against the herpes simplex 2 virus (HSV-2).

PRODUCTION OF BLENDED JUICE FROM PUMPKIN, QUINCE, PERSIMMON

2018 ◽  
pp. 59-62
Author(s):  
I. A. Kyazimova ◽  
А. А. Kasumova ◽  
А. А. Nabiev
Keyword(s):  
Phenolic Compounds ◽  
Organic Acids ◽  
New Technology ◽  
Mineral Elements ◽  
Biologically Active ◽  
Active Components ◽  
Aliphatic Acids ◽  
Free Glucose ◽  
Dietary Value ◽  
Quantitative Indicators

Production of plant products, including juices around the world increases continuously. In the fruit and vegetable juices contain a significant amount of monosaccharides (glucose and fructose), organic acids, vitamins, phenolic compounds, mineral substances and other biologically active components that determine the nutritional and dietary value. For the prevention of various diseases associated with impaired metabolic processes, we developed a new technology of preparation of food by blending juice of pumpkin, quince and persimmon. Thus prepared organic blended juice contains a substantial amount of free glucose and fructose, different phenolic compounds, a sufficient amount of organic acids, mineral elements, including iodine and other components that determine its nutritional and biological value. In prepared juices were evaluated the quantitative indicators of β-carotene, vitamin C, glucose and fructose, sucrose, starch, pectin substances. Also in the atomic absorbtion spectrometer Analyst 400 (PerkinElmer, USA) was analyzed content of the organic acids and phenolic compounds. Prepared juices were tested in accordance with 10 point scoring scale. It is established that all juices contain a sufficient amount of the minerals. In pumpkin and quince juices not contain iodine while it presents in sufficient amount in persimmon juice that’s why in the blended juice mineral in addition to mineral elements iodine are contained. In pumpkin and persimmon aliphatic acids are contained in small amount. For this reason during the blending process was used quince juice which is rich in aliphatic acids. The blended juice is light straw color, with delicious flavor, a slight astringent property and a balanced taste.

Fast Method for the Determination of Residual Solvents in Radiopharmaceutical Products

2017 ◽  
Vol 68 (4) ◽  
pp. 666-670 ◽  
Author(s):  
Mirela Mihon ◽  
Catalin Stelian Tuta ◽  
Alina Catrinel Ion ◽  
Dana Niculae ◽  
Vasile Lavric
Keyword(s):  
Gas Chromatography ◽  
Control Method ◽  
Limit Of Detection ◽  
The Body ◽  
Biologically Active ◽  
Injection Technique ◽  
Fast Method ◽  
Residual Solvent ◽  
Residual Solvents

The aim of this work was the development and validation of a fast analytical method to determine the residual solvents content in radiopharmaceuticals such as: 18F-Fluorodeoxyglucose (18F-FDG), 18F-Fluoroestradiol (18F-FES), 18F-Fluorothymidine (18F-FLT),18F-Fluoromisonidazole (18F-FMISO). Radiopharmaceuticals are radioactive preparations for medical purposes used in nuclear medicine as tracers in diagnostic imaging and treatment of certain diseases. Positron Emission Tomography (PET) is a medical imaging technique that consists in introducing into the body of a small amount of a biologically active chemical compound labelled with a short lived positron-emitting radioisotope (18F, 11C, 68Ga). Residual solvents are critical impurities in radiopharmaceuticals that can affect labelling, stability and physicochemical properties of drugs. Therefore, the determination of these solvents is essential for quality control of radiopharmaceuticals. Validation of the control method for residual solvents by gas chromatography is referred by the European Pharmacopoeia using a special injection technique (head space). The parameters of the method, which comply with International Conference on Harmonization guidelines, are: accuracy, precision, linearity, limit of detection, limit of quantification and robustness. The proposed method (direct gas chromatography injection) proved to be linear, precise, accurate and robust. Good linearity was achieved for all the solvents and correlation coefficients (R2) for each residual solvent were found more than 0.99.

Simultaneous Determination of Six Components in Jingzhiguanxin Tablet by High-Performance Liquid Chromatography

2019 ◽  
Vol 15 (2) ◽  
pp. 130-137
Author(s):  
Hui Jiang ◽  
Lianhao Fu ◽  
Yu Wang ◽  
Shaozhi Wang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Tanshinone Iia ◽  
Array Detector ◽  
Control Analysis ◽  
Active Components ◽  
Quality Control Analysis

Background: Jingzhiguanxin (JZGX) tablet, a traditional Chinese prescription, is commonly used for treating coronary heart disease and angina pectoris in the clinic. There are six active components (Danshensu (DSS), Protocatechuic aldehyde (PD), Paeoniflorin (PF), Ferulic acid (FA), Salvianolic acid B (Sal B) and Tanshinone IIA (TA)) in JZGX tablet. </P><P> Objective: In this paper, a simple and reliable method was used for simultaneous determining the six active components by high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Methods: These six active components were separated on an Agilent Zorbax Eclipse XDB-C18 column (150 mmx4.6 mm, 5 µm) at 30 °C. Acetonitrile (A), methanol (B) and 0.5% H3PO4 aqueous solution (C) were used as mobile phase for gradient elution. The flow rate was 1 mL/min and the detection wavelengths were set at 280 nm for DSS, PD and Sal B, 230 nm for PF, 320 nm for FA and 270 nm for TA, respectively. Results: All of the six components showed good linearity regressions (r2≥0.9997) in the detected concentration range. The recovery rates and coefficient of variation (CV) for all analytes were 98.66%- 100.18% and 0.75%-1.89%, respectively. This method was successfully applied to simultaneously determine the six components in JZGX tablet from different batches and manufacturers. Conclusion: The validated method can be used in routine quality control analysis of JZGX tablet without any interference.

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