Isolation of a pharmacologically active principle from ehrlich carcinoma tumor cells

1971 ◽  
Vol 27 (8) ◽  
pp. 947-949
Author(s):  
B. Shohat ◽  
H. Joshua
Keyword(s):  
Tumor Cells ◽  
Ehrlich Carcinoma ◽  
Active Principle ◽  
Pharmacologically Active ◽  
Carcinoma Tumor

ANTITUMOR EFFECT OF COLLOIDAL SILVER BISILICATE IN EXPERIMENTAL IN VITRO AND IN VIVO STUDIES

2019 ◽  
Vol 65 (5) ◽  
pp. 760-765
Author(s):  
Margarita Tyndyk ◽  
Irina Popovich ◽  
A. Malek ◽  
R. Samsonov ◽  
N. Germanov ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Human Tumor ◽  
Significant Inhibition ◽  
In Vivo Studies ◽  
Ehrlich Carcinoma ◽  
In Vivo Experiments

The paper presents the results of the research on the antitumor activity of a new drug - atomic clusters of silver (ACS), the colloidal solution of nanostructured silver bisilicate Ag6Si2O7 with particles size of 1-2 nm in deionized water. In vitro studies to evaluate the effect of various ACS concentrations in human tumor cells cultures (breast cancer, colon carcinoma and prostate cancer) were conducted. The highest antitumor activity of ACS was observed in dilutions from 2.7 mg/l to 5.1 mg/l, resulting in the death of tumor cells in all studied cell cultures. In vivo experiments on transplanted Ehrlich carcinoma model in mice consuming 0.75 mg/kg ACS with drinking water revealed significant inhibition of tumor growth since the 14th day of experiment (maximally by 52% on the 28th day, p < 0.05) in comparison with control. Subcutaneous injections of 2.5 mg/kg ACS inhibited Ehrlich's tumor growth on the 7th and 10th days of the experiment (p < 0.05) as compared to control.

Targeting Protective Catalase of Tumor Cells with Cold Atmospheric Plasma- Activated Medium (PAM)

2018 ◽  
Vol 18 (6) ◽  
pp. 784-804 ◽  
Author(s):  
Georg Bauer
Keyword(s):  
Control System ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Plasma Potential ◽  
Atmospheric Plasma ◽  
Antitumor Action ◽  
Active Principle ◽  
Reaction Products ◽  
Cold Atmospheric Plasma

Background: Application of cold atmospheric plasma to medium generates “plasma-activated medium” that induces apoptosis selectively in tumor cells and that has an antitumor effect in vivo. The underlying mechanisms are not well understood. Objective: Elucidation of potential chemical interactions within plasma-activated medium and of reactions of medium components with specific target structures of tumor cells should allow to define the active principle in plasma activated medium. Methods: Established knowledge of intercellular apoptosis-inducing reactive oxygen/nitrogen species-dependent signaling and its control by membrane-associated catalase and SOD was reviewed. Model experiments using extracellular singlet oxygen were analyzed with respect to catalase inactivation and their relevance for the antitumor action of cold atmospheric plasma. Potential interactions of this tumor cell-specific control system with components of plasma-activated medium or its reaction products were discussed within the scope of the reviewed signaling principles. Results: None of the long-lived species found in plasma-activated medium, such as nitrite and H2O2, nor OCl- or .NO seemed to have the potential to interfere with catalase-dependent control of apoptosis-inducing signaling of tumor cells when acting alone. However, the combination of H2O2 and nitrite might generate peroxynitrite. The protonation of peroxnitrite to peroxynitrous acid allows for the generation of hydroxyl radicals that react with H2O2, leading to the formation of hydroperoxide radicals. These allow for singlet oxygen generation and inactivation of membrane-associated catalase through an autoamplificatory mechanism, followed by intercellular apoptosis-inducing signaling. Conclusion: Nitrite and H2O2 in plasma-activated medium establish singlet oxygen-dependent interference selectively with the control system of tumor cells.

scholarly journals RANK Ligand Modulation of Autophagy in Oral Squamous Cell Carcinoma Tumor Cells

2015 ◽  
Vol 117 (1) ◽  
pp. 118-125 ◽  
Author(s):  
Yuvaraj Sambandam ◽  
Sashank Sakamuri ◽  
Sundaravadivel Balasubramanian ◽  
Azizul Haque
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Rank Ligand ◽  
Squamous Cell Carcinoma Tumor ◽  
Carcinoma Tumor

The metabolism of α-2′ -deoxythioguanosine in murine tumor cells

1968 ◽  
Vol 46 (7) ◽  
pp. 655-661 ◽  
Author(s):  
G. A. LePage
Keyword(s):  
Cell Growth ◽  
Tumor Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Carcinoma Cells ◽  
Ehrlich Carcinoma ◽  
Human Blood Cells ◽  
Murine Tumor Cells ◽  
Rna And Dna

Preparations of α-2′-deoxythioguanosine (α-TGdR) completely free of β-2′-deoxythioguanosine (β-TGdR) were made and labeled with radiosulfur. A purified preparation of nucleoside phosphorylase obtained from human blood cells was found to be active on the β-anomer but completely inactive on the α-anomer. This property of the enzyme was used to characterize metabolites of α-TGdR. α-TGdR and β-TGdR were both found to be converted to the corresponding mono-, di-, and tri-phosphates and incorporated into the nucleic acids of Mecca lymphosarcoma cells in vivo. α-TGdR appeared predominantly in the terminal nucleoside positions of RNA and DNA. β-TGdR appeared predominantly in the nucleotide chain. Both Mecca lymphosarcoma and Ehrlich carcinoma cells formed significant quantities of analog nucleotides at all three levels of phosphorylation, probably exceeding the levels of guanine nucleotides in the cells for an appreciable time. The findings further support a correlation reported earlier between the incorporation of 6-thioguanine into DNA and the inhibition of cell growth.

UV-specific DNA repair recombinant fusion enzyme: A new stable pharmacologically active principle suitable for photoprotection

2005 ◽  
Vol 39 (2) ◽  
pp. 81-88
Author(s):  
Guillermo Basílico ◽  
Carolina Alvarez Roger ◽  
Mauricio Seigelchifer ◽  
Nestor Kerner
Keyword(s):  
Dna Repair ◽  
Active Principle ◽  
Fusion Enzyme ◽  
Pharmacologically Active

Programming the anti-tumor immune response in vitro and its application to stop the growth of tumor cells and prolonging the lifespan of mice with carcinoma in vivo

2017 ◽  
pp. 67-73
Author(s):  
С.В. Калиш ◽  
С.В. Лямина ◽  
А.А. Раецкая ◽  
О.П. Буданова ◽  
И.Ю. Малышев
Keyword(s):  
Immune Response ◽  
Tumor Growth ◽  
Tumor Cells ◽  
The Body ◽  
Ehrlich Carcinoma ◽  
Ec Cells ◽  
Growth Of Tumor ◽  
Immune Response In Vitro

Цель - представить доказательства правомерности гипотезы, что комбинированный пул репрограммированных in vitro макрофагов и лимфоцитов будет эффективно ограничивать пролиферацию опухолевых клеток in vitro , а при введении в организм будет существенно ограничивать развитие опухоли in vivo . Методика. Размножение опухолевых клеток инициировали in vitro путем добавления клеток карциномы Эрлиха (КЭ) в среду культивирования RPMI-1640. Развитие асцитной опухоли in vivo воспроизводили путем внутрибрюшной инъекции клеток КЭ мышам. Результаты. Установлено, что M3 макрофаги вместе с антиген-репрограммированными лимфоцитами оказывают выраженный противоопухолевый эффект и in vitro, и in vivo , который был существеннее противоопухолевого эффекта цисплатина. Заключение. Факты, свидетельствующие, что М3 макрофаги в сочетании с in vitro антиген-репрограммированными лимфоцитами значительно подавляют рост опухоли in vivo , делают перспективным разработку клинической версии биотехнологии ограничения роста опухоли путем предварительного программирования противоопухолевого иммунного ответа «в пробирке». Aim. To test a hypothesis that a combined pool of in vitro reprogrammed macrophages and lymphocytes will effectively limit growth of tumor cells in vitro , and injections of these cells into the body will considerably limit development of a tumor in vivo . Methods. Tumor growth was initiated in vitro by addition of Ehrlich carcinoma (EC) cells to the RPMI-1640 cell culture medium and in vivo by intraperitoneal injection of EC cells into mice. Results. M3 macrophages in combination with antigen-reprogrammed lymphocytes exerted a pronounced antitumor effect both in vitro and in vivo, which was superior to the effect of cisplatin. Conclusion. M3 macrophages in combination with in vitro antigen-reprogrammed lymphocytes significantly inhibited the tumor growth in vivo . This fact justifies development of a clinical version of the tumor growth restricting biotechnology using pre-programming of the antitumor immune response in vitro .

Sign in / Sign up

Export Citation Format

Share Document