scholarly journals Root-Associated Mycobiomes of Common Temperate Plants (Calluna vulgaris and Holcus lanatus) Are Strongly Affected by Winter Climate Conditions

2021 ◽  
Author(s):  
Mathilde Borg Dahl ◽  
Derek Peršoh ◽  
Anke Jentsch ◽  
Jürgen Kreyling
Keyword(s):  
Soil Temperature ◽  
Plant Species ◽  
High Throughput Sequencing ◽  
Calluna Vulgaris ◽  
Absolute Temperature ◽  
Snow Accumulation ◽  
Temperature Variability ◽  
Holcus Lanatus ◽  
Soil Frost ◽  
Winter Climate

AbstractWinter temperatures are projected to increase in Central Europe. Subsequently, snow cover will decrease, leading to increased soil temperature variability, with potentially different consequences for soil frost depending on e.g. altitude. Here, we experimentally evaluated the effects of increased winter soil temperature variability on the root associated mycobiome of two plant species (Calluna vulgaris and Holcus lanatus) at two sites in Germany; a colder and wetter upland site with high snow accumulation and a warmer and drier lowland site, with low snow accumulation. Mesocosm monocultures were set-up in spring 2010 at both sites (with soil and plants originating from the lowland site). In the following winter, an experimental warming pulse treatment was initiated by overhead infrared heaters and warming wires at the soil surface for half of the mesocosms at both sites. At the lowland site, the warming treatment resulted in a reduced number of days with soil frost as well as increased the average daily temperature amplitude. Contrary, the treatment caused no changes in these parameters at the upland site, which was in general a much more frost affected site. Soil and plant roots were sampled before and after the following growing season (spring and autumn 2011). High-throughput sequencing was used for profiling of the root-associated fungal (ITS marker) community (mycobiome). Site was found to have a profound effect on the composition of the mycobiome, which at the upland site was dominated by fast growing saprotrophs (Mortierellomycota), and at the lowland site by plant species-specific symbionts (e.g. Rhizoscyphus ericae and Microdochium bolleyi for C. vulgaris and H. lanatus respectively). The transplantation to the colder upland site and the temperature treatment at the warmer lowland site had comparable consequences for the mycobiome, implying that winter climate change resulting in higher temperature variability has large consequences for mycobiome structures regardless of absolute temperature of a given site.

scholarly journals Increased winter soil temperature variability enhances nitrogen cycling and soil biotic activity in temperate heathland and grassland mesocosms

2014 ◽  
Vol 11 (23) ◽  
pp. 7051-7060 ◽  
Author(s):  
J. Schuerings ◽  
A. Jentsch ◽  
V. Hammerl ◽  
K. Lenz ◽  
H. A. L. Henry ◽  
...  
Keyword(s):  
Nutrient Cycling ◽  
Soil Temperature ◽  
Nitrogen Cycling ◽  
N Uptake ◽  
Snow Accumulation ◽  
Temperature Variability ◽  
Temperate Zone ◽  
N Leaching ◽  
N Availability ◽  
Winter Warming

Abstract. Winter air temperatures are projected to increase in the temperate zone, whereas snow cover is projected to decrease, leading to increased soil temperature variability, and potentially to changes in nutrient cycling. Here, we experimentally evaluated the effects of increased winter soil temperature variability on selected aspects of the N-cycle in mesocosms containing different plant community compositions. The experiment was replicated at two sites, a colder mountainous upland site with high snow accumulation and a warmer and drier lowland site. Increased soil temperature variability enhanced soil biotic activity for both sites during winter, as indicated by 35% higher nitrogen (N) availability in the soil solution, 40% higher belowground decomposition and a 25% increase in the potential activity of the enzyme cellobiohydrolase. The mobilization of N differed between sites, and the 15N signal in leaves was reduced by 31% in response to winter warming pulses, but only at the cold site, with significant reductions occurring for three of four tested plant species at this site. Furthermore, there was a trend of increased N leaching in response to the recurrent winter warming pulses. Overall, projected winter climate change in the temperate zone, with less snow and more variable soil temperatures, appears important for shifts in ecosystem functioning (i.e. nutrient cycling). While the effects of warming pulses on plant N mobilization did not differ among sites, reduced plant 15N incorporation at the colder temperate site suggests that frost damage may reduce plant N uptake in a warmer world, with important implications for nitrogen cycling and nitrogen losses from ecosystems.

scholarly journals Ecosystem Processes Show Uniform Sensitivity to Winter Soil Temperature Change Across a Gradient from Central to Cold Marginal Stands of a Major Temperate Forest Tree

Ecosystems ◽  
2021 ◽  
Author(s):  
Robert Weigel ◽  
Hugh A. L. Henry ◽  
Ilka Beil ◽  
Gerhard Gebauer ◽  
Gerald Jurasinski ◽  
...  
Keyword(s):  
Climate Change ◽  
Soil Temperature ◽  
Snow Cover ◽  
Tree Growth ◽  
Forest Tree ◽  
Beech Forest ◽  
Soil Frost ◽  
Winter Climate ◽  
Climate Change Effects ◽  
Winter Climate Change

AbstractThe magnitude and frequency of soil frost events might increase in northern temperate regions in response to climate warming due to reduced insulation caused by declining snow cover. In temperate deciduous forests, increased soil frost severity can hamper tree growth and increase the mortality of fine roots, soil fauna and microorganisms, thus altering carbon and nutrient cycling. From single-site studies, however, it is unclear how the sensitivities of these responses change along continental gradients from regions with low to high snowfall. We conducted a gradient design snow cover and soil temperature manipulation experiment across a range of lowland beech forest sites to assess the site-specific sensitivity of tree growth and biogeochemical cycling to soil cooling. Even mild and inconsistent soil frost affected tree increment, germination, litter decomposition and the retention of added 15N. However, the sensitivity of response (treatment effect size per degree of warming or cooling) was not related to prevailing winter climate and snow cover conditions. Our results support that it may be valid to scale these responses to simulated winter climate change up from local studies to regional scales. This upscaling, however, needs to account for the fact that cold regions with historically high snowfall may experience increasingly harsh soil frost conditions, whereas in warmer regions with historically low snowfall, soil frost may diminish. Thus, despite the uniform biotic sensitivity of response, there may be opposing directions of winter climate change effects on temperate forests along continental temperature gradients due to different trends of winter soil temperature.

scholarly journals Illuminating the Plant Rhabdovirus Landscape through Metatranscriptomics Data

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1304
Author(s):  
Nicolás Bejerman ◽  
Ralf G. Dietzgen ◽  
Humberto Debat
Keyword(s):  
Plant Species ◽  
High Throughput Sequencing ◽  
Plant Viruses ◽  
The Novel ◽  
Coding Regions ◽  
Public Data ◽  
Invaluable Tool ◽  
Sequencing Platforms ◽  
Viral Sequences ◽  
Plant Rhabdovirus

Rhabdoviruses infect a large number of plant species and cause significant crop diseases. They have a negative-sense, single-stranded unsegmented or bisegmented RNA genome. The number of plant-associated rhabdovirid sequences has grown in the last few years in concert with the extensive use of high-throughput sequencing platforms. Here, we report the discovery of 27 novel rhabdovirus genomes associated with 25 different host plant species and one insect, which were hidden in public databases. These viral sequences were identified through homology searches in more than 3000 plant and insect transcriptomes from the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) using known plant rhabdovirus sequences as the query. The identification, assembly and curation of raw SRA reads resulted in sixteen viral genome sequences with full-length coding regions and ten partial genomes. Highlights of the obtained sequences include viruses with unique and novel genome organizations among known plant rhabdoviruses. Phylogenetic analysis showed that thirteen of the novel viruses were related to cytorhabdoviruses, one to alphanucleorhabdoviruses, five to betanucleorhabdoviruses, one to dichorhaviruses and seven to varicosaviruses. These findings resulted in the most complete phylogeny of plant rhabdoviruses to date and shed new light on the phylogenetic relationships and evolutionary landscape of this group of plant viruses. Furthermore, this study provided additional evidence for the complexity and diversity of plant rhabdovirus genomes and demonstrated that analyzing SRA public data provides an invaluable tool to accelerate virus discovery, gain evolutionary insights and refine virus taxonomy.

scholarly journals Projected decrease in wintertime bearing capacity on different forest and soil types in Finland under a warming climate

2019 ◽  
Vol 23 (3) ◽  
pp. 1611-1631 ◽  
Author(s):  
Ilari Lehtonen ◽  
Ari Venäläinen ◽  
Matti Kämäräinen ◽  
Antti Asikainen ◽  
Juha Laitila ◽  
...  
Keyword(s):  
Bearing Capacity ◽  
21St Century ◽  
Climate Model ◽  
Thick Layer ◽  
Timber Harvesting ◽  
Snow Accumulation ◽  
Point Of View ◽  
Frozen Soil ◽  
Soil Types ◽  
Soil Frost

Abstract. Trafficability in forest terrain is controlled by ground-bearing capacity, which is crucial from the timber harvesting point of view. In winter, soil frost affects the most the bearing capacity, especially on peatland soils which have in general low bearing capacity. Ground frost similarly affects the bearing capacity of forest truck roads. A 20 cm thick layer of frozen soil or 40 cm thick layer of snow on the ground may already be sufficient for heavy forest harvesters. In this work, we studied the impacts of climate change on soil frost conditions and, consequently, on ground-bearing capacity from the timber harvesting point of view. The number of days with good wintertime bearing capacity was modelled by using a soil temperature model with a snow accumulation model and wide set of downscaled climate model data until the end of the 21st century. The model was calibrated for different forest and soil types. The results show that by the mid-21st century, the conditions with good bearing capacity will decrease in wintertime in Finland, most likely by about 1 month. The decrease in soil frost and wintertime bearing capacity will be more pronounced during the latter half of the century, when drained peatlands may virtually lack soil frost in most of winters in southern and western Finland. The projected decrease in the bearing capacity, accompanied with increasing demand for wood harvesting from drained peatlands, induces a clear need for the development of sustainable and resource-efficient logging practices for drained peatlands. This is also needed to avoid unnecessary harvesting damages, like rut formation on soils and damage to tree roots and stems.

scholarly journals Impact of Subsurface Temperature Variability on Surface Air Temperature Variability: An AGCM Study

2008 ◽  
Vol 9 (4) ◽  
pp. 804-815 ◽  
Author(s):  
Sarith P. P. Mahanama ◽  
Randal D. Koster ◽  
Rolf H. Reichle ◽  
Max J. Suarez
Keyword(s):  
Surface Temperature ◽  
Soil Temperature ◽  
Air Temperature ◽  
Seasonal Cycle ◽  
Surface Air Temperature ◽  
Temperature Variability ◽  
Climate System ◽  
Boreal Summer ◽  
Temperature Anomalies ◽  
Subsurface Soil

Abstract Anomalous atmospheric conditions can lead to surface temperature anomalies, which in turn can lead to temperature anomalies in the subsurface soil. The subsurface soil temperature (and the associated ground heat content) has significant memory—the dissipation of a temperature anomaly may take weeks to months—and thus subsurface soil temperature may contribute to the low-frequency variability of energy and water variables elsewhere in the system. The memory may even provide some skill to subseasonal and seasonal forecasts. This study uses three long-term AGCM experiments to isolate the contribution of subsurface soil temperature variability to variability elsewhere in the climate system. The first experiment consists of a standard ensemble of Atmospheric Model Intercomparison Project (AMIP)-type simulations in which the subsurface soil temperature variable is allowed to interact with the rest of the system. In the second experiment, the coupling of the subsurface soil temperature to the rest of the climate system is disabled; that is, at each grid cell, the local climatological seasonal cycle of subsurface soil temperature (as determined from the first experiment) is prescribed. Finally, a climatological seasonal cycle of sea surface temperature (SST) is prescribed in the third experiment. Together, the three experiments allow the isolation of the contributions of variable SSTs, interactive subsurface soil temperature, and chaotic atmospheric dynamics to meteorological variability. The results show that allowing an interactive subsurface soil temperature does, indeed, significantly increase surface air temperature variability and memory in most regions. In many regions, however, the impact is negligible, particularly during boreal summer.

Endophytic root bacteria associated with the natural vegetation growing at the hydrocarbon-contaminated Bitumount Provincial Historic site

2017 ◽  
Vol 63 (6) ◽  
pp. 502-515 ◽  
Author(s):  
Natalie P. Blain ◽  
Bobbi L. Helgason ◽  
James J. Germida
Keyword(s):  
Plant Species ◽  
Contaminated Soils ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Growth Promotion ◽  
Plant Root ◽  
Gene Copy ◽  
Sampling Location ◽  
Historic Site ◽  
Copy Numbers

The Bitumount Provincial Historic site is the location of 2 of the world’s first oil-extracting and -refining operations. Despite hydrocarbon levels ranging from 330 to 24 700 mg·(kg soil)−1, plants have been able to recolonize the site through means of natural revegetation. This study was designed to achieve a better understanding of the plant-root-associated bacterial partnerships occurring within naturally revegetated hydrocarbon-contaminated soils. Root endophytic bacterial communities were characterized from representative plant species throughout the site by both high-throughput sequencing and culturing techniques. Population abundance of rhizosphere and root endosphere bacteria was significantly influenced (p < 0.05) by plant species and sampling location. In general, members of the Actinomycetales, Rhizobiales, Pseudomonadales, Burkholderiales, and Sphingomonadales orders were the most commonly identified orders. Community structure of root-associated bacteria was influenced by both plant species and sampling location. Quantitative real-time polymerase chain reaction was used to determine the potential functional diversity of the root endophytic bacteria. The gene copy numbers of 16S rRNA and 2 hydrocarbon-degrading genes (CYP153 and alkB) were significantly affected (p < 0.05) by the interaction of plant species and sampling location. Our findings suggest that some of the bacterial communities detected are known to exhibit plant growth promotion characteristics.

scholarly journals Identification and Profiling of Conserved MicroRNAs in Different Developmental Stages of Crown Imperial (Fritillaria Imperialis L.) using High-throughput Sequencing

2021 ◽  
Author(s):  
Fereshteh Ahmadi Teshniz ◽  
Behrouz Shiran ◽  
Sadegh Mousavi-Fard ◽  
Hossein Fallahi ◽  
Bojana Banović Đeri
Keyword(s):  
Plant Species ◽  
Abiotic Stresses ◽  
Regulatory Networks ◽  
High Throughput Sequencing ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Practical Application ◽  
Sequencing Technologies ◽  
New Strategies ◽  
Different Tissues

Abstract Novel strategies for improvement of plants’ ornamental and other properties relay on miRNA control of differential plant gene expression modulation. Still, in response to the same abiotic stresses, some conserved miRNA families show different expression patterns in different plant species. In parallel, the use of deep sequencing technologies reveals new levels of complexity of regulatory networks in plants through identification of new miRNAs. These are two major reasons why more studies are needed before envisioned new strategies may take their course in practical application domain. This research revealed 21 conserved miRNAs, matching 15 miRNA families, in Fritilaria imperialis. Among identified conserved miRNA families in crown imperial, miR166, miR169 and miR396 families were the most abundant ones. The expression of seven conserved miRNAs (Fim-miR156b, Fim-miR159, Fim-miR166a-5p, Fim-miR169d-5p, Fim-miR171c, Fim-miR393 and Fim-miR396e-3p) was further investigated in different tissues and three developmental stages, suggesting different roles these miRNAs have in growth and development of crown imperial. Gained knowledge from this research can open the door to find efficient ways to secure crown imperial survival, preservation and utilization and if proven useful may be applied in other plant species as well.

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