A Global Proteomic Change in Petroleum Hydrocarbon-Degrading Pseudomonas aeruginosa in Response to High and Low Concentrations of Petroleum Hydrocarbons

2019 ◽  
Vol 76 (11) ◽  
pp. 1270-1277 ◽  
Author(s):  
Jun-Di Wang ◽  
Xu-Xiang Li ◽  
Cheng-Tun Qu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Low Concentrations ◽  
Proteomic Change

scholarly journals Spatial Distribution of Total Petroleum Hydrocarbon (TPH) in Sediment of Sumba Sea, Nusa Tenggara Timur

2018 ◽  
Vol 3 (2) ◽  
pp. 173
Author(s):  
Deny Yogaswara ◽  
Khozanah Khozanah
Keyword(s):  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Outer Boundary ◽  
Total Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbons ◽  
Sampling Station ◽  
Tectonic Plate ◽  
Low Concentrations ◽  
Infra Red ◽  
Partial Distribution

Sumba Sea is an Indo-Australian tectonic plate transition zone that has a huge biodiversities resources and also behalf of an international shipping lane zone from southern of Indonesia to Australia and New Zealand, and as the return. In addition, Sumba Sea is also a kind of outer boundary of Indonesia, that is necessary to do basic environmental monitoring as the authorities in the management of outer sea zone. Indonesia does not have representative environmental quality database including the pollution of Total Petroleum Hydrocarbons (TPH). This study purposes to determine the partial distribution and concentration of TPH in sediments in Sumba Sea, East Nusa Tenggara. The research was conducted in August 2016 using Research Vessel of Baruna Jaya VIII. Samples were collected using a box core, preserved in amber glass jar bottle and stored at 4°C for further analysis in the laboratory. In the laboratory, sediment samples were extracted using dichloromethane and n-hexane for three times extraction. Furthermore, samples were evaporated before added by tetrachloroethylene solvent. Samples were measured with Fourier Transform Infra Red (FTIR) at wavelength 2850-2950 cm<sup>-1</sup>. The results showed that the pollution of Total Petroleum Hydrocarbon (TPH) in the Sumba Sea was detected at all sampling station based on identified of hydrocarbon functional groups. The partial distribution of TPH is evenly distributed and covered all stations on low concentrations. The highest concentration of TPH was detected at station 10 as 4.348 ppm

scholarly journals Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

2020 ◽  
pp. 1-7
Author(s):  
Williams, Janet Olufunmilayo ◽  
Aleruchi Owhonka
Keyword(s):  
Mixed Culture ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbons ◽  
Aspergillus Sydowii ◽  
Significant Difference ◽  
Set Up ◽  
Rivers State

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l.  The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.

scholarly journals OIL SPILL INCIDENTS AND DISPERSANT APPLICATIONS IN KUWAIT

1987 ◽  
Vol 1987 (1) ◽  
pp. 247-253
Author(s):  
Salah M. Al-Mazidi ◽  
Omar Samhan
Keyword(s):  
Oil Spill ◽  
Marine Environment ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Petroleum Products ◽  
Coastal Environment ◽  
Industrial Activities ◽  
Oil Refineries ◽  
North And South

ABSTRACT Since the discovery of oil in Kuwait, most oil-related activities have been located along the coastline 50 km south of Kuwait City. Other related industrial activities have been developed in this area apart from oil and petroleum products export in order to diversify the national sources of income. For these reasons, the potential for large oil spills in Kuwait's marine environment is highest along the south coast, where oil refineries and exporting facilities are located. An average of 219 barrels of oil were spilled annually between 1979 and 1985, and 2,100 gallons of dispersants were used in cleanup operations. The majority of incidents involved less than 5 barrels of oil and 500 gallons of dispersants. Incidents involving more than 100 barrels of oil and 5,000 gallons of dispersants were confined to the Sea Island and Mina Al-Ahmadi North and South Piers. This distribution undoubtedly affects the concentration of petroleum residues in various components of the marine environment, resulting in an increase in tar ball density along this coast, reaching a maximum at Ras Az-Zor, and significantly higher levels of vanadium and petroleum hydrocarbons in sediments and oysters collected south of Mina Al-Ahmadi. The objective of this paper is to report on the number, volume, and frequency distribution of oil spill incidents in Kuwait and the usage of dispersants in cleanup operations. Vanadium and petroleum hydrocarbon concentrations also are described as is the sensitivity of the southern coastal environment to oil spills. Recommendations have been made on how to conduct cleanup operations for any future oil spill incidents along the southern shoreline of Kuwait.

scholarly journals MIKROORGANISME PENDEGRADASI TPH (TOTAL PETROLEUM HYDROCARBON) SEBAGAI AGEN BIOREMEDIASI TANAH TERCEMAR MINYAK BUMI (Review Article)

2018 ◽  
Vol 2 (2) ◽  
pp. 35
Author(s):  
Prasetyo Handrianto
Keyword(s):  
Petroleum Hydrocarbons ◽  
Soil Chemistry ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Oil Pollution ◽  
Review Article ◽  
Total Petroleum Hydrocarbon ◽  
Soil Conditions ◽  
Petroleum Pollution ◽  
Ph Optimization

Exploitation and exploration activities will produce sewage sludge and crude oil spills that cause pollution to the environment and upgrading to the environment, biology and soil chemistry. Monitoring of oil pollution conditions on the soil can be done by detection of all hydrocarbon components, or what is called the total petroleum hydrocarbon (TPH). According to its components, this total petroleum hydrocarbon (TPH) can be classified into 3 points, aliphatic, alicyclic, and aromatic. One of the biological efforts that can be used to overcome petroleum pollution is by using bioremediation technology. There are several methods in bioremediation, one of which is the biostimulation method, where the growth of the original hydrocarbon decomposers is stimulated by adding nutrients, oxygen, pH optimization and temperature. Hydrocarbonoclastic microorganisms have characteristic not possessed by other microorganisms, namely their ability to excrete hydroxylase enzymes, which are hydrocarbon oxidizing enzymes, so that these bacteria can degrade petroleum hydrocarbons. Biodegradation can be formed if there is a structural transformation so that cahnges in molecular integrity occur. This process is a series of enzymatic or biochemical reaction that require ideal environmental conditions with the growth and proliferation of microorganisms. Something that need to be known before remediation are pollutants (organic or inorganic), degraded/ not, dangerous/ not, how many pollutants pollute the soil, the ratio of carbon (C), Nitrogen (N), and phophorus (P), soil type, soil conditions (wet dry), and how long pollutants have been deposited in these locations

Hydrogeochemical Mechanism of the Petroleum Hydrocarbon Pollution in Karst Fissure Groundwater System

2013 ◽  
Vol 295-298 ◽  
pp. 159-163 ◽  
Author(s):  
Zhen Min Ma ◽  
Yun Yun Luo ◽  
Yun Zhi Fang ◽  
Yu Song Hou
Keyword(s):  
Water Quality ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Water System ◽  
Quality Parameters ◽  
Groundwater System ◽  
Hydrocarbon Pollution ◽  
Groundwater Environment ◽  
Before And After ◽  
Fissure Water

The research of hydrogeochemical mechanism of petroleum hydrocarbon in karst fissure groundwater system is important to predict the trend of petroleum hydrocarbons and the change of groundwater environment. We take the karst fissure water system as the research object, where there is a refinery. The variation of SO42-, HCO3-, NO3-, NO2-, HS- can be used as a hydrogeochemical sign of petroleum hydrocarbon pollution by analyzing the change of water quality parameters before and after karst fissure water contaminated by petroleum hydrocarbon. It has been also analyzed systematically that hydrogeochemical mechanism including desulfurization, denigration and ion exchange happen during the pollution process in the karst fissure water system. It is pointed out that the human activities have a great impact on the groundwater and changes of environment.

Oil Degrading Bacteria: Remediation of Environmental Pollution Resulting from Petroleum Hydrocarbons

2020 ◽  
Vol 2 (10) ◽  
pp. 5-10
Author(s):  
Ishita Agrawal
Keyword(s):  
Environmental Pollution ◽  
Environmental Health ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
High Efficiency ◽  
Bacterial Species ◽  
Ongoing Research ◽  
Degrading Bacteria ◽  
Remedial Actions ◽  
Hazardous Pollutants

It is widely known that petroleum hydrocarbons constitute one of the most hazardous pollutants that affect human and environmental health. The ongoing research on bioremediation with petroleum hydrocarbon-degrading bacteria has shown tremendous promise of the technology due to its advantages of high efficiency and eco-friendly nature. To this end, studies have been carried out to identify a large amount of bacterial species with petroleum hydrocarbon-degrading ability for applications in bioremediation. Here, we present a brief perspective of some of the notable advances in oil degrading bacteria and the remedial actions for decontamination of water and soil along with recovering the spilled materials at oil sites.

scholarly journals Detection of endotoxins with the Limulus test in burned and unburned mice infected with different species of gramnegative bacteria

1975 ◽  
Vol 75 (1) ◽  
pp. 99-112 ◽  
Author(s):  
R. J. Jones ◽  
E. A. Roe ◽  
R. E. Dyster
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Low Concentrations ◽  
High Concentrations ◽  
Very High ◽  
Limulus Test

SUMMARYThe Limulus test detected endotoxins in the plasma of burned and unburned mice infected with different species of gram-negative bacteria. Individual strains of different species of gram-negative bacteria produced different amounts of endotoxin in the plasma of infected mice. Plasma from mice given lethal infections showed very high concentrations of endotoxin. Low concentrations of endotoxin in the plasma were tolerated by mice but high concentrations were invariably fatal. A polyvalent pseudomonas vaccine reduced endotoxin in the plasma of mice given lethal infections of Pseudomonas aeruginosa.

Interiaboratory Evaluation of an Off-Line Supercritical Fluid Extraction/Infrared Spectrometric Method for Determination of Petroleum Hydrocarbons in Solid Matrixes

1993 ◽  
Vol 76 (3) ◽  
pp. 555-564 ◽  
Author(s):  
Viorica Lopez-Avila ◽  
Richard Young ◽  
Robert Kim ◽  
Werner F Beckert ◽  
◽  
...  
Keyword(s):  
Fourier Transform ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Clay Soil ◽  
Fluid Extraction ◽  
Accuracy And Precision ◽  
Fourier Transform Ir ◽  
Method Accuracy

Abstract A collaborative study was conducted, with 14 laboratories participating, to determine the method accuracy and precision of the proposed U.S. Environmental Protection Agency Methods 3560 and 8440. These methods involve the extraction of petroleum hydrocarbons from solid matrixes with supercritical carbon dioxide at 340 atm and 80°C for 30 min (dynamic), collection of the extracted materials in tetrachloroethene (Method 3560), and analysis of the extracts by infrared (IR) spectrometry (Method 8440). The study design was based on the AOAC blind replicate design with balanced replicates. The study samples consisted of 4 solid matrixes that had petroleum hydrocarbon contents ranging from 614 to 32 600 mg/kg. Each of the 4 matrixes was extracted in triplicate, and the extracts were analyzed with 2 different IR spectrometers. In addition, each of the participating laboratories extracted a sample of unspiked clay soil, the same clay soil spiked with corn oil and reference oil at 1000 mg/kg each, and the same clay soil wetted to 30% water content and spiked with motor oil at 10 000 mg/kg (the latter 3 samples were extracted only once). Results indicated that the overall method accuracy for concentrations ranging from 614 to 32 600 mg/kg was 82.9%; the mean recoveries of petroleum hydrocarbons for each of the 4 solid matrixes ranged from 77.9 to 107% for analyses performed with the Perkin-Elmer Fourier transform IR spectrometer and from 75.9 to 101% for analyses performed with the Buck-Scientific IR spectrometer; the differences between the 2 instruments on a sample-bysample basis were less than 17% for the total petroleum hydrocarbon determinations. The interiaboratory method precisions (RSDR) appeared to be matrix-dependent and ranged from 17.3 to 45.4% for analyses performed with the Perkin- Elmer Fourier transform IR spectrometer and from 16.7 to 47.9% for the Buck-Scientific IR spectrometer. The intralaboratory method precisions (RSDr) appeared to be less matrix-dependent and ranged from 11.5 to 17.0% for analyses performed with the Perkin-Elmer FTIR spectrometer and from 11.1 to 18.2% for the Buck-Scientific IR spectrometer. Method accuracy and precision data are also presented for the 5 laboratories that used Isco supercritical fluid extraction systems and for the 7 laboratories that used vessels with volumes of 3.5 mL or less with different supercritical fluid extraction systems.

scholarly journals Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

2020 ◽  
Vol 26 (5) ◽  
pp. 200384-0
Author(s):  
Jianbo Liu ◽  
Liming Xu ◽  
Feifei Zhu ◽  
Shouhao Jia
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Initial Conditions ◽  
Removal Rate ◽  
Surface Hydrophobicity ◽  
Orthogonal Experiments ◽  
Petroleum Contaminated Soil ◽  
Application Potential ◽  
High Removal Rate

It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.

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