Virulent gene profile and antimicrobial susceptibility pattern of Shiga toxin-producing Escherichia coli (STEC) from humans in Maiduguri, Borno State, North-Eastern Nigeria

2017 ◽  
Vol 27 (2) ◽  
pp. 341-351 ◽  
Author(s):  
Musa Sakuma Adamu ◽  
Iniobong Chukwuebuka Ikenna Ugochukwu ◽  
Sunday Idoko Idoko ◽  
Yakubu Adamu Kwabugge ◽  
Nafisatu Sa’ad Abubakar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Shiga Toxin ◽  
Susceptibility Pattern ◽  
Gene Profile ◽  
Antimicrobial Susceptibility Pattern ◽  
North Eastern ◽  
Virulent Gene

Virulent gene profile and antibiotic susceptibility pattern of Shiga toxin-producing Escherichia coli (STEC) from cattle and camels in Maiduguri, North-Eastern Nigeria

2018 ◽  
Vol 50 (6) ◽  
pp. 1327-1341 ◽  
Author(s):  
Musa Sakuma Adamu ◽  
Iniobong Chukwuebuka Ikenna Ugochukwu ◽  
Sunday Idoko Idoko ◽  
Yakubu Adamu Kwabugge ◽  
Nafisatu Sa’ad Abubakar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Antibiotic Susceptibility ◽  
Susceptibility Pattern ◽  
Gene Profile ◽  
Antibiotic Susceptibility Pattern ◽  
North Eastern ◽  
Virulent Gene

scholarly journals Isolation, Identification, and Antimicrobial Susceptibility Pattern of Shiga toxin-producing Escherichia Coli O157:H7 from Food of Bovine Origin in Mekelle, Tigray, Ethiopia

2021 ◽  
Author(s):  
Million Weldeselassie ◽  
Getachew Gugsa ◽  
Yisehak Tsegaye ◽  
Nesibu Awol ◽  
Ashwani Kumar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Shiga Toxin ◽  
Molecular Techniques ◽  
Animal Origin ◽  
Susceptibility Pattern ◽  
Cross Sectional ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern ◽  
Food Of Animal Origin

Abstract Background: Escherichia coli O157:H7 is an emerging and major zoonotic foodborne pathogen with an increasing concern for the emergence and spread of antimicrobial-resistant strains which may results in sporadic cases to serious outbreaks in the whole world. Cattle have been identified as a major reservoir of the pathogen. This study aimed to isolate and characterize Shiga toxin-producing E. coli O157:H7 from raw milk, yogurt, and meat of bovine origin and determine their antimicrobial susceptibility pattern. Methods: A cross-sectional study was conducted from December 2014-June 2015 and a total of 284 milk and meat were collected from different sources in Mekelle. The collected samples were analyzed for the presence of E. coli and Shiga toxin-producing E. coli O157:H7 and determination of their antimicrobial susceptibility pattern following the standard bacteriological and molecular techniques and procedures, and antimicrobial sensitivity test. Results: Out of the total 284 samples, 70(24.64%) were positive bacteriologically to E. coli and 14.29% were found to be Shiga toxin-producing E. coli O157:H7. All (100%) E. coli isolates carried the pal and 41.67% eae gene (EHEC). Of these EHEC isolates 40% and 60% were positive for stx1 and stx2, respectively. E. coli isolates were showed the highest level of sensitivity for Gentamycin (91.7%) but the highest level of resistance to Amoxicillin (95.8%). Of the tested isolates, 18(75%) of E. coli showed multiple antimicrobial resistance.Conclusions: The current study revealed the occurrence of Shiga toxin-producing E. coli O157:H7 in foods of bovine origin in the study area. So, there is a chance of acquiring infection via the consumption of raw or undercooked food of bovine origin. Thus, awareness creation should be made on foodborne disease caused by Shiga toxin-producing E. coli O157:H7 with due consideration on the safe handling and consumption of food of animal origin.

scholarly journals Frequency and antimicrobial susceptibility pattern of hospital isolates of Escherichia coli and Klebsiella pneumoniae in urine samples

2019 ◽  
Vol 49 ◽  
Author(s):  
Ivana Jukić ◽  
Danijel Topić ◽  
El-Jesah Đulić ◽  
Amela Dedeić- Ljubović
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Public Health Problem ◽  
Urine Samples ◽  
Distribution Analysis ◽  
Susceptibility Pattern ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern ◽  
Urinary Infections ◽  
Tract Infections

Objectives/Aim: Urinary tract infections (UTI) are a serious public health problem and caused by many pathogens, most often by Escherichia coli and Klebsiella pneumoniae.Aim of this study was to show the frequency and antimicrobial susceptibility pattern of E. coli and K. pneumoniae in hospital isolates, following the GLASS methodology.Methods: This is a retrospective study that was conducted in the Laboratory for Urinary Infections OU Clinical Microbiology CCUS in the period from January till December 2018.A total of 13760 urine samples were processed, using standard laboratory methods, in which significant bacteriuria was detected in 3218 (23.4%) of specimens.Results: Out of the total number of positive samples, E. coli was isolated in 1166 (36.2%) and K. pneumoniae at 341 (10.6%) patients. The presence of E. coli and K. pneumoniae isolates is dominant in females (1103/73.2%) and age group 60 and older (812/54.7%). Out of the total E. coli isolates, the ESBL strain was presented with 79 (6.8%) and K. pneumoniae with 145 (42.5%). The proportion of carbapenemase (CPE) produced by K. pneumoniae isolates was 8 (2.4%). The ESBL strain distribution analysis on clinics showed the highest prevalence of both isolates at the Clinic for Nephrology: K. pneumoniae 26/17,9%; E. coli: 12/15.4%. Isolated E. coli showed the highest resistance to ampicillin 673/1166 (57,7%), trimethoprim-sulfamethoxazole 454/1166 (38.9%) and ciprofloxacin 253/970 (26.1%), while K. pneumoniae to ciprofloxacin and trimethoprim-sulfamethoxazole with 151/285 (53.0%) and 164/341 (48.1%), respectively.Conclusions: Our study has shown that the most common cause of urinary infections in hospital settings are E. coli and K. pneumoniae. Data analysis showed that the presence of ESBL isolates was significantly higher in K. pneumoniae than E. coli. CPE isolates of K. pneumoniae were also detected.

scholarly journals Antimicrobial Susceptibility Pattern and ESBL Prevalence of Bacteria Isolated from Street Vended Snacks

2021 ◽  
pp. 74-80
Author(s):  
Chidiebere Anab-Atulomah ◽  
Ngozi C. Oji ◽  
Vivian N. Kanu
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Laboratory Unit ◽  
Antimicrobial Susceptibility Pattern ◽  
Significant Difference ◽  
Esbl Producers

Aims: The objective of the study was to ascertain the antimicrobial susceptibility pattern and ESBL prevalence of bacteria isolated from snacks. Place and Duration of Study: Department of Microbiology (Laboratory Unit) Michael Okpara University of Agriculture Umudike. Methodology: The snacks were mashed aseptically, serially diluted and inoculated onto nutrient agar and MacConkey agar. Isolates were identified using standard microbiological procedures. Antimicrobial susceptibility of the isolates and ESBL detection was done using disk diffusion method. ESBL production was confirmed using Double Disc Synergy Test (DDST) method following CLSI recommendations. Results: Escherichia coli, Salmonella Typhi, Staphylococcus aureus and Klebsiella pneumoniae were the bacteria isolated with Escherichia coli as the most prevalent isolate with 42% occurrence in the samples screened. There was significant difference in the sensitivity of the bacteria isolates to the different antibiotics used at P=0.05. Salmonella Typhi isolates exhibited highest resistance to an antibiotic with 86% resistance to ciprofloxacin while Klebsiella pneumoniae isolates exhibited the lowest resistance to an antibiotic with 10% resistance to cefotaxime. Among the Gram-negative bacteria, 36% of suspected ESBL producing E. coli isolates were confirmed as ESBL producers indicating the highest occurrence. Conclusion: The study confirmed the presence of bacteria in street vended snacks which exhibited high resistance to antibiotics that could be attributed to the presence of ESBL producers among the isolates.

scholarly journals Antimicrobial susceptibility pattern of urine culture isolates in a tertiary care hospital of Jharkhand, India

2017 ◽  
Vol 6 (7) ◽  
pp. 1733 ◽  
Author(s):  
Akhilesh Kumar ◽  
Rajiv Kumar ◽  
Manju Gari ◽  
Uma Shanker P. Keshri ◽  
Sumit K. Mahato ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Urine Culture ◽  
Tertiary Care ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
The Common

Background: Urinary tract infection (UTI) is one of the common infections encountered by the clinicians. Though a good number of antimicrobial agents are available, still UTIs have become difficult to treat due to development of resistance by the uropathogens. So, regional data regarding the common uropathogens and their sensitivity pattern is required to guide the clinicians to start empirical therapy while managing UTIs. The purpose of the study was to identify different species of microorganisms, along with their antimicrobial susceptibility pattern, causing urinary tract infection in outpatient and indoor patients at RIMS, Ranchi, Jharkhand.Methods: Observational study was conducted using urine culture and sensitivity reports collected retrospectively from records maintained in the department of Microbiology over a period from July 2016 to Feb 2017 in tertiary care hospital.Results: UTI was more common in females (57.74%) than in males (42.26%). Among the uropathogens isolated Escherichia coli (37.41%) was found to be the predominant organism followed by Klebsiella species (32.79%), Pseudomonas species (25.86%), and gram-positive bacteria Staphylococcus aureus accounted (3.92%) of total cases. The most common isolates were E. coli showed high sensitivity to amikacin (79.24%), followed by levofloxacin (77.21%) and gentamycin (62.26%). It was found to be resistant to norfloxacin (86%), nalidixic acid (86.76%) and cefotaxime (69.88%).Conclusions: Though various microorganisms are responsible for UTI. Escherichia coli species is the most common organism. Antimicrobial resistance has already emerged against many antibiotics, making empirical treatment of these infections challenging.

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