Calcium alginate matrix increases the stability and recycling capability of immobilized endo-β-1,4-xylanase from Geobacillus stearothermophilus KIBGE-IB29

Extremophiles ◽  
2015 ◽  
Vol 19 (4) ◽  
pp. 819-827 ◽  
Author(s):  
Zainab Bibi ◽  
Shah Ali Ul Qader ◽  
Afsheen Aman
Author(s):  
Rosetânia Correia Neves da Conceição ◽  
Rayssa Dias Batista ◽  
Fernanda Munhoz dos Anjos Leal Zimmer ◽  
Ianna Kelly Martins Trindade ◽  
Alex Fernando de Almeida ◽  
...  

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153685 ◽  
Author(s):  
Shen-Fu Lin ◽  
Ying-Chen Chen ◽  
Ray-Neng Chen ◽  
Ling-Chun Chen ◽  
Hsiu-O Ho ◽  
...  

2005 ◽  
Vol 16 (1) ◽  
pp. 9-13 ◽  
Author(s):  
R. HURTEAUX ◽  
H. Benhayoune ◽  
F. Edwards-Levy ◽  
S. Bouthors ◽  
G. Balossier ◽  
...  

Biologia ◽  
2010 ◽  
Vol 65 (3) ◽  
Author(s):  
Shuvashish Behera ◽  
Rama Mohanty ◽  
Ramesh Ray

AbstractMahula (Madhuca latifolia L.) is a deciduous tree commonly found in the tropical rain forests of Asian and Australian continent. Corolla, the edible part of its flowers, is rich in fermentable sugar (37 ± 0.23%; on dry weight basis). Batch fermentation of mahula flowers was carried out using Zymomonas mobilis MTCC 92 free cells and cells immobilized in calcium alginate matrix. The ethanol productions were 122.9 ± 0.972 and 134.6 ± 0.104 g/kg flowers on dry weight basis using free and immobilized cells, respectively, after 96 h of fermentation, which showed that cells entrapped in calcium alginate matrix yielded 8.7% more ethanol than free cells. Further, the immobilized cells were physiologically active up to three more cycles of fermentation producing 132.7 ± 0.095, 130.5 ± 0.09 and 128.7 ± 0.056 g ethanol per kg flower in first, second and third cycle, respectively.


2016 ◽  
Vol 21 (2) ◽  
pp. 437-442
Author(s):  
Mario Andres Forero-Cujiño ◽  
Luis Carlos Montengro Ruiz ◽  
Gabriel Antonio Pinilla-Agudelo ◽  
Luz Marina Melgarejo-Muñoz

<p>En este trabajo se describe la técnica de inmovilización de microalgas en esferas de alginato de calcio. Se emplearon las especies Scenedesmus ovalternus y Chlorella vulgaris, se determinó la estabilidad de las esferas, la cinética de crecimiento y la concentración de las microalgas en el interior de las esferas. Chlorella vulgaris alcanzó mayores densidades poblacionales y tasas de crecimiento más altas cuando se inmovilizó en concentraciones del 10 % v/v con el alginato (1,31*10<span>6 </span>cél/ml). Para Scenedesmus ovalternus se observó una mayor densidad poblacional y una mayor tasa de crecimiento cuando se inmovilizó en concentraciones del 20 % v/v (7,06*10<span>5 </span>cél/ml). Estos resultados son útiles para aplicaciones prácticas de las algas encapsuladas, tales como el biomonitoreo o la biorremediación.</p><p><strong>Immobilization of Microalgae<em> Scenedesmus ovalternus</em> (Scenedesmaceae) and <em>Chlorella vulgaris</em> (Chlorellaceae) in Calcium Alginate Beads</strong></p><p>This paper describes the immobilization technique of microalgae in calcium alginate beads. Scenedesmus ovalternus and Chlorella vulgaris species were used. The stability of beads, the kinetics of growth and the concentrations of microalgae inside the beads were determined. The higher density and the upper growth rate of Chlorella vulgaris occurred when it was immobilized in alginate at a concentration of 10 %v/v (1,31*10<span>6 </span>cél/ml). Scenedesmus ovalternus achieved a higher population density and an elevated growth rate when it was immobilized at a concentration of 20 % v/v (7,06*10<span>5 </span>cél/ml). These results are useful for subsequent applications of the encapsulated algae, such as biomonitoring and bioremediation.</p>


2017 ◽  
Vol 2 (4) ◽  
pp. 10 ◽  
Author(s):  
Rahmath Abdulla ◽  
Warda Abdul Ajak ◽  
Siti Hajar ◽  
Eryati Derman

Currently the resources for fossil fuels are depleting together with increase in fuel prices. This has urged the need for cheaper alternative fuels especially biofuels. The production of the most common liquid biofuel which is bioethanol using immobilized yeast cells is an approach taken to increase its demand in the world’s market. There are various methods for the immobilization of yeast cells; however before they can be applied in the industry the stability of the immobilization technology must be investigated. This research aims to study the stabilities of immobilized S. cerevisiae in calcium alginate and carrageenan beads for bioethanol production. The S. cerevisiae was immobilized in calcium alginate and carrageenan beads using entrapment method. Next, screening for the optimal concentration of sodium alginate and semi refined carrageenan matrices were determined by employing fermentation and bioethanol quantification using GC-MS. Concentrations of 2% (w/v) calcium alginate and 2% (w/v) semi refined carrageenan beads were identified to produce the highest bioethanol yield which were 0.286 g/mL and 0.065 g/mL respectively. The two beads were then chosen to be tested in various stability studies with respect to bioethanol production such as storage stability, reusability, pH, thermaland permeability test. It was found out that a concentration of 2% (w/v) calcium alginate beads were more stable as immobilization matrix for S. cerevisiae  as compared to 2% (w/v) semi refined carrageenan.


2008 ◽  
Vol 28 (2) ◽  
pp. 253-257 ◽  
Author(s):  
M.A. Morales ◽  
P.V. Finotelli ◽  
J.A.H. Coaquira ◽  
M.H.M. Rocha-Leão ◽  
C. Diaz-Aguila ◽  
...  

Polymers ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 170
Author(s):  
Lăcrămioara Rusu ◽  
Cristina-Gabriela Grigoraș ◽  
Andrei-Ionuț Simion ◽  
Elena-Mirela Suceveanu ◽  
Alexandra-Cristina Blaga ◽  
...  

Pharmaceuticals are recognized as emerging water microcontaminants that have been reported in several aquatic environments worldwide; therefore, the elimination of these pollutants is a global challenge. This study aimed to develop a biosorbent based on Saccharomyces pastorianus residual biomass encapsulated in a calcium alginate matrix and to evaluate its biosorption performance to remove Ethacridine Lactate (EL) from aqueous solutions. Firstly, the synthesis and characterization of biosorbent has been carried out. Then, the impact of main parameters on biosorption process were investigated by batch experiments. Finally, the kinetics behavior and equilibrium isotherms were evaluated. The resulted beads have an irregular and elongated shape with about 1.89 mm ± 0.13 mm in size with a homogeneous structure. The best removal efficiency for EL of over 85% was obtained at acidic pH 2 and 25 °C for 50 mg/L initial concentration and 2 g/L biosorbent dose. The pseudo-second-order and intraparticle diffusion kinetics describe the biosorption process. The maximum calculated biosorption capacity was 21.39 mg/g similar to that recorded experimentally. The equilibrium biosorption data were a good fit for Freundlich and Dubinin–Radushkevich isotherms. Our findings reveal that the low cost and eco-friendly obtained biosorbent can be easily synthesized and suitable to remove Ethacridine Lactate from water matrices.


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