scholarly journals Mapping QTLs for tolerance to salt stress at the early seedling stage in rice (Oryza sativa L.) using a newly identified donor ‘Madina Koyo’

Euphytica ◽  
2020 ◽  
Vol 216 (10) ◽  
Author(s):  
Nana Kofi Abaka Amoah ◽  
Richard Akromah ◽  
Alex Wireko Kena ◽  
Baboucarr Manneh ◽  
Ibnou Dieng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Oryza Sativa L ◽  
Dry Weight ◽  
Interval Mapping ◽  
Snp Markers ◽  
Seedling Stage ◽  
Direct Selection ◽  
Chromosome 2 ◽  
Shoot Dry Weight ◽  
Early Seedling

Abstract Salt stress is a menace to rice production and a threat to food security worldwide. We evaluated 308 F4 families from Sahel 317/Madina Koyo for tolerance to salt stress at the early seedling stage. To better understand genomic regions controlling tolerance in the population, we genotyped the progenies and the two parents using single nucleotide polymorphism (SNP) markers and regressed the genotypic data on their phenotype to detect QTLs. An average reduction of 63.4% was observed for all fitness-related traits among the F4 families. A total of 46 progenies recorded an average salt injury score (SIS) between 1–3 and were rated as tolerant to salt stress at the early seedling stage. A high-density genetic map was constructed for the 12 rice chromosomes using 3698 SNP markers. Multiple interval mapping identified 13 QTLs for SIS, shoot length, shoot dry weight and root length on chromosomes 2, 3, 4, 6, 7, 10 and 12, with trait increasing alleles coming from both parents. Two (qSDW2 and qRL2.2) and three (qSL2, qRL2.1 and qSIS2) QTLs at different regions on chromosome 2 and another two on chromosome 7 (qSDW7 and qSL7) were tightly linked. These QTLs could facilitate breeding for salt tolerance at the early seedling stage as direct selection for one, would mean indirectly selecting for the other. Fine mapping of these novel QTLs in a different genetic background is necessary to confirm their stability and usefulness in breeding for tolerance to salinity in rice.

scholarly journals Selection of drought tolerant wheat genotypes by osmotic stress imposed at germination and early seedling stage

2018 ◽  
Vol 15 (2) ◽  
pp. 177-192
Author(s):  
RR Saha ◽  
A Hannan ◽  
A Nessa ◽  
MA Malek ◽  
MR Islam
Keyword(s):  
Osmotic Stress ◽  
Dry Weight ◽  
Seedling Stage ◽  
Metabolic Efficiency ◽  
Wheat Genotypes ◽  
Shoot Dry Weight ◽  
Vigour Index ◽  
Peg Treatment ◽  
Early Seedling ◽  
Different Levels

An experiment on hundred wheat genotypes under different levels of osmotic stress was carried out during 2014 to select the genotype(s) tolerant to drought at germination and early seedling stage. Different levels of osmotic stress were imposed by using polyethylene glycol (PEG). Three osmotic stress levels viz. control (distilled water), 15% PEG solution and 25% PEG solution were used. Among the 100 genotypes the rate of germination percentage, final germination (%), root and shoot dry weight, amount of respiration and vigour index under PEG treatment was found significantly lower than that of control condition. Compared to control condition relative decrease in rate of germination, final germination, amount of respiration and vigour index among the wheat genotypes were found more at 25% PEG than that of 15% PEG treatment. However, the seed metabolic efficiency was significantly higher in wheat genotypes under both 15% PEG and 25% PEG treatment compared to the control condition. A significant positive correlation exists between the important growth parameters like rate of germination (%), final germination (%), shoot dry weight, root dry weight and vigour index. On the basis of these physiological traits against osmotic stress, nine genotypes of wheat such as BD-480, BD-498, BD- 501, BD-513, BD-514, BD-519, BD-592, BD-618 and BD- 633 were selected as drought tolerant.SAARC J. Agri., 15(2): 177-192 (2017)

scholarly journals Physiological Evaluation of Different Rice Genotypes to Two Salinity Level during Seedling Stage under Hydroponic System

2020 ◽  
pp. 42-56
Author(s):  
S. Lakshmi ◽  
V. Ravichandran ◽  
L. Arul ◽  
K. Krishna Surendar
Keyword(s):  
Salt Stress ◽  
Dry Weight ◽  
Seedling Stage ◽  
Salinity Level ◽  
Ion Concentration ◽  
Growth And Yield ◽  
Root Shoot Ratio ◽  
Ion Absorption ◽  
Early Seedling ◽  
Rice Genotypes

Hydroponics study was conducted to screen eight rice genotypes (CO 51, ADT 53, ADT 37, IR 64, CO 43, ASD 16, Pokkali; TRY 3) under salinity stress on early seedling stage. Two Saline treatments (75 and 100 mM NaCl) were given at 15 days old seedling; observations were recorded at 10 days after salt stress. Results showed that shoot length, root length, total fresh and dry weight, shoot and root fresh weight, shoot and root dry weight and root- shoot ratio were reduced under saline conditions compared to control. Na+ ion Concentration and Na+/ k+ ratio was higher in saline treatments than control. However, K+ ion absorption decreased with increasing salinity level. Electrolyte leakage and osmotic potential had increasing trend with increasing level of salinity. In this study, rice genotypes Pokkali, TRY 3 and CO 43 perform as tolerant; CO 51, ADT 53 and ASD 16 perform as moderately tolerant; ADT 37 is susceptible and IR 64 is highly susceptible. This type of study is required to develop salt tolerant genotypes at salt stress during seedling stage; to increase the growth and yield of rice there by satisfy the need of country’s requirement.

Physiological response of chickpea (Cicer arietinum L.) at early seedling stage under salt stress conditions

2019 ◽  
Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Seedling Growth ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Seedling Stage ◽  
Tolerance Index ◽  
Early Seedling Growth ◽  
Early Seedling

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.

scholarly journals Evaluation of Rice (Oryza sativa L.) Genotypes at Germination and Early Seedling Stage for Their Tolerance to Salinity

1970 ◽  
Vol 8 (2) ◽  
pp. 57-65 ◽  
Author(s):  
MM Islam ◽  
MA Karim
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Dry Weight ◽  
Seedling Stage ◽  
Saline Condition ◽  
Salt Tolerant ◽  
Early Seedling ◽  
Rice Genotypes ◽  
Tolerance To Salinity ◽  
Cacl2 Solution

An experiment was conducted at the Laboratory of Agronomy Department, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur from July-June, 2009-2010 to evaluate the rice (Oryza sativa L.) genotypes for their tolerance to salinity. One hundred rice genotypes and two check cultivars (Pokkali as tolerant and IR29 as susceptible) were exposed to solution of Electrical Conductivity (EC) of 10, 15, and 20 dSm-1 (5:1 molar concentration of NaCl and CaCl2 solution) at germination and early seedling stage. Based on visual salt injury symptoms at 15 dSm-1, 13 genotypes were found fairly tolerant to salinity. However, among the 13 genotypes, only Patnai23 showed higher germination index and seedling relative dry weight than the check salt tolerant Pokkali at 15 dSm-1. Beside these, performance of Awned-1, Nonasail and Soloi was also well at this level. The genotypes Patnai23, Awned-1, Nonasail and Soloi showed the best performance under saline condition. Keywords: Rice genotypes; germination; salinity; tolerance DOI: 10.3329/agric.v8i2.7578   The Agriculturists 8(2): 57-65 (2010)   

scholarly journals Antioxidative and Metabolic Contribution to Salinity Stress Responses in Two Rapeseed Cultivars during the Early Seedling Stage

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1227
Author(s):  
Ali Mahmoud El-Badri ◽  
Maria Batool ◽  
Ibrahim A. A. Mohamed ◽  
Zongkai Wang ◽  
Ahmed Khatab ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Stress Responses ◽  
Antioxidant Activities ◽  
Tricarboxylic Acid Cycle ◽  
Seedling Stage ◽  
Plant Performance ◽  
Salt Tolerant ◽  
Brassica Napus L ◽  
Early Seedling

Measuring metabolite patterns and antioxidant ability is vital to understanding the physiological and molecular responses of plants under salinity. A morphological analysis of five rapeseed cultivars showed that Yangyou 9 and Zhongshuang 11 were the most salt-tolerant and -sensitive, respectively. In Yangyou 9, the reactive oxygen species (ROS) level and malondialdehyde (MDA) content were minimized by the activation of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) for scavenging of over-accumulated ROS under salinity stress. Furthermore, Yangyou 9 showed a significantly higher positive correlation with photosynthetic pigments, osmolyte accumulation, and an adjusted Na+/K+ ratio to improve salt tolerance compared to Zhongshuang 11. Out of 332 compounds identified in the metabolic profile, 225 metabolites were filtrated according to p < 0.05, and 47 metabolites responded to salt stress within tolerant and sensitive cultivars during the studied time, whereas 16 and 9 metabolic compounds accumulated during 12 and 24 h, respectively, in Yangyou 9 after being sown in salt treatment, including fatty acids, amino acids, and flavonoids. These metabolites are relevant to metabolic pathways (amino acid, sucrose, flavonoid metabolism, and tricarboxylic acid cycle (TCA), which accumulated as a response to salinity stress. Thus, Yangyou 9, as a tolerant cultivar, showed improved antioxidant enzyme activity and higher metabolite accumulation, which enhances its tolerance against salinity. This work aids in elucidating the essential cellular metabolic changes in response to salt stress in rapeseed cultivars during seed germination. Meanwhile, the identified metabolites can act as biomarkers to characterize plant performance in breeding programs under salt stress. This comprehensive study of the metabolomics and antioxidant activities of Brassica napus L. during the early seedling stage is of great reference value for plant breeders to develop salt-tolerant rapeseed cultivars.

scholarly journals Response of Iranian Rice Recombinant Inbred Lines (Oryza sativa L.) to Salt Stress in Seedling Stage

2019 ◽  
Vol 11 (29) ◽  
pp. 65-84
Author(s):  
Seyedeh Minoo Mirarab Razi ◽  
Reza Shirzadian-Khorramabad ◽  
Hossein Sabouri ◽  
Babak Rabiei ◽  
Hossein Hosseini Moghadam ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Recombinant Inbred ◽  
Oryza Sativa L ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Seedling Stage

scholarly journals Evaluation of Rice (Oryza sativa L.) Genotypes for Drought Tolerance at Germination and Early Seedling Stage

2018 ◽  
Vol 16 (1) ◽  
pp. 44-54 ◽  
Author(s):  
M M Islam ◽  
E Kayesh ◽  
E Zaman ◽  
T A Urmi ◽  
M M Haque
Keyword(s):  
Oryza Sativa ◽  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Evaluation System ◽  
Oryza Sativa L ◽  
Dry Weight ◽  
Seedling Height ◽  
Early Seedling ◽  
Rice Genotypes

Drought stress is a major constraint to the production and yield stability of crops. Rice (Oryza sativa L.) is considered as a drought-sensitive crop species. Within this species, there are considerable varietal differences in sensitivity to this environmental stress. An experiment was conducted at the laboratory of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during April to September 2016 to evaluate 100 rice genotypes for drought tolerance during germination and early seedling growth stage. The genotypes were tested against five levels of drought stress imposed by Polyethylene glycol 6000 (PEG-6000) @ 0, 5, 10, 15 and 20%. The experiment was laid out in a complete randomized design with four replications. The results showed that with increasing water stress, germination in all the genotypes decreased from 95.8% in control to 6.6 % in highest stress (20% PEG) level. Seedling height and dry weight also decreased in all rice genotypes with the increase in water stress level. Based on Standard Evaluation System (SES),18 genotypes were selected primarily. Among the 18 genotypes, Ratoil and Chinisakkar showed higher germination index, relative seedling height and relative seedling dry weight than the check drought tolerant BRRI dhan43 at 20% PEG. Beside these, performance of Kumridhan, Pusur and Somondori was also well at this level compared to BRRI dhan43. The genotypes Ratoil, Chinisakkar, Kumridhan, Pusur and Somondori showed the best performance under drought condition. The Agriculturists 2018; 16(1) 44-54

scholarly journals QTLs for seedling traits under salinity stress in hexaploid wheat

2018 ◽  
Vol 48 (3) ◽  
Author(s):  
Yongzhe Ren ◽  
Yanhua Xu ◽  
Wan Teng ◽  
Bin Li ◽  
Tongbao Lin
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Hexaploid Wheat ◽  
Dry Weight ◽  
Interval Mapping ◽  
Mapping Method ◽  
Stress Conditions ◽  
Wheat Varieties ◽  
Seedling Traits

ABSTRACT: Soil salinity limits agricultural production and is a major obstacle for increasing crop yield. Common wheat is one of the most important crops with allohexaploid characteristic and a highly complex genome. QTL mapping is a useful way to identify genes for quantitative traits such as salinity tolerance in hexaploid wheat. In the present study, a hydroponic trial was carried out to identify quantitative trait loci (QTLs) associated with salinity tolerance of wheat under 150mM NaCl concentration using a recombinant inbred line population (Xiaoyan 54×Jing 411). Values of wheat seedling traits including maximum root length (MRL), root dry weight (RDW), shoot dry weight (SDW), total dry weight (TDW) and the ratio of TDW of wheat plants between salt stress and control (TDWR) were evaluated or calculated. A total of 19QTLs for five traits were detected through composite interval mapping method by using QTL Cartographer version 2.5 under normal and salt stress conditions. These QTLs distributed on 12 chromosomes explained the percentage of phenotypic variation by individual QTL varying from 7.9% to 19.0%. Among them, 11 and six QTLs were detected under normal and salt stress conditions, respectively and two QTLs were detected for TDWR. Some salt tolerance related loci may be pleiotropic. Chromosome 1A, 3A and 7A may harbor crucial candidate genes associated with wheat salt tolerance. Our results would be helpful for the marker assisted selection to breed wheat varieties with improved salt tolerance.

scholarly journals Pemberian Pupuk Silika Cair untuk Meningkatkan Pertumbuhan, Hasil, dan Toleransi Kekeringan Padi Sawah

2018 ◽  
Vol 46 (2) ◽  
pp. 153 ◽  
Author(s):  
Sugiyanta , ◽  
I Made Dharmika ◽  
Dan Dedeh Siti Mulyani
Keyword(s):  
Field Experiment ◽  
Soil Water ◽  
Stress Responses ◽  
Oryza Sativa L ◽  
Block Design ◽  
Dry Weight ◽  
Fertilizer Application ◽  
Greenhouse Experiment ◽  
Growth And Yield ◽  
Shoot Dry Weight

ABSTRACT<br />    <br />As one of the silica (Si) accumulator, rice (Oryza sativa L.) requires large amounts of silica for growth. This study aimed to determine the effect of liquid silica fertilizer application on lowland rice growth and yield, and its roles in drought stress responses. The experiments were conducted at Sawah Baru rice field and Cikabayan Greenhouse Experiment Station, IPB, Bogor during the period of January to May 2016. The field experiment was set up in a randomized complete block design with 7 treatments and three replications. The greenhouses experiment was arranged in a split-plot randomized block design with 3 replications. The main plots were 5 levels of liquid silica fertilizer application, while the subplot was 5 level of various soil water contents. The results of the field experiment showed that the application of liquid silica fertilizer increased rice shoot dry weight, total and productive tiller numbers, and yield per plant, but did not increase yield per hectare. Meanwhile at greenhouse experiment, it showed that the use of liquid silica fertilizer reduced the number of rice stomata, but did not increase cuticle thickness and grain yield per plant. Silica application was not effective to alter drought in rice.<br /><br />Keywords: IPB 3S, silica fertilizer, soil water content, water use efficiency   <br /><br />

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