Impact of microalgae culture conditions over the capacity of copper nanoparticle biosynthesis

2019 ◽  
Vol 31 (4) ◽  
pp. 2437-2447 ◽  
Author(s):  
Gerardo Salas-Herrera ◽  
Susana González-Morales ◽  
Adalberto Benavides-Mendoza ◽  
Adali O. Castañeda-Facio ◽  
Fabián Fernández-Luqueño ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Copper Nanoparticle ◽  
Microalgae Culture ◽  
Nanoparticle Biosynthesis

Preliminary Study on Climate Change Biomitigation by Improving CO2 Removal and CO2 Utilization Efficiency Using Microalgae Culture in Photobioreactor

2015 ◽  
Vol 747 ◽  
pp. 261-264
Author(s):  
Astri Nugroho ◽  
Edwan Kardena ◽  
Dea Indriani Astuti ◽  
Kania Dewi
Keyword(s):  
Removal Efficiency ◽  
Gas Flow ◽  
Biological Process ◽  
Culture Conditions ◽  
Utilization Efficiency ◽  
Biological Fixation ◽  
Microalgae Culture ◽  
Light Darkness ◽  
Preliminary Study ◽  
Microalgae Growth

Culture conditions are very important to CO2 bio-fixation related with the CO2 removal efficiency through biological process by microalgae photosynthesis activities. The aim of the research was to study how high CO2 utilization efficiency could reach in mix culture that supplied high CO2 concentration (2%, 5%, and 7%) continuously from the bottom of photobioreactor. The mix microalgae culture containing of Chlorella sp, Scenedesmusobliquus and Ankistrodemus sp. were cultivated in photobioreactor with various environmental treatments i.e light intensities, light periodism and temperatures whereas the fixed CO2 gas flow rate of 8 L.min-1. The results showed that microalgae growth was best at light intensity of 4000 lux for 16/8 hours light/darkness cycling, 30°C and 5% CO2 supplied, indicated by the highest dried biomass (g.L-1), the highest Carbon content was g.d-1 and highest CO2 removal efficiency (%) that were 2.7, 11.9, 49, respectively. However the highest CO2 utilization efficiency for bio-fixation phenomenon was obtained from culture that supplied by 2% CO2 concentration, the value was almost 2 fold than 5% CO2 supplied and 4 fold than 7% CO2 concentration supplied, respectively. Biological fixation of CO2 are greatly affected by the characteristics of the microalgae strains and their tolerance to environmental conditions.

Five Objective Optimization Using Naïve & Sorting Genetic Algorithm (NSGA) for Green Microalgae Culture Conditions for Biodiesel Production

2019 ◽  
Vol 12 (2) ◽  
pp. 110-121
Author(s):  
Jujjavarapu Satya Eswari ◽  
Manwendra Kumar Tripathi ◽  
Swasti Dhagat ◽  
Santosh Kr. Karn
Keyword(s):  
Genetic Algorithm ◽  
Chlorophyll Content ◽  
Culture Conditions ◽  
Biodiesel Production ◽  
Surface Model ◽  
Multi Objective ◽  
Chlorella Sp ◽  
Microalgae Culture ◽  
Optimization Stage ◽  
Chlorophyll Protein

Background: Renewable sources of energy like biodiesel are substitute energy fuel which are made from renewable bio sources or biomasses. Due to many advantages of using algae (Chlorella sp), we performed design of experiments in terms of functional and biochemical factors such as biomass, chlorophyll content, protein moiety and carbohydrate and lipid contents. Objective: Our objective is maximization of lipid accumulation (y1) and chlorophyll content (y2) and minimization of carbohydrate consumption (y3), protein (y4) and biomass (y5) contents. By using the experimental data, the regression model has been developed in order to obtain the desired response (biomass, chlorophyll, protein, carbohydrate and lipid) therefore it is necessary to optimize input conditions. The pre-optimization stage is an important part and useful for the production of biodiesel as biomass which is renewable energy to improve the quality. Methodology: The corresponding input and output conditions with multi-objective optimisation using naïve & sorting genetic algorithm (NSGA) is X1=0.99, X2=0.001, X3=-1.111, X4=0.01 and Lipid= 42.34, Chlorophyll=1.1212 (µgmL-1), Carbohydrate= 24.54%, Protein= 0.0742 (mgmL-1), Biomass=0.999 (gL-1). Conclusion: The multi-objective optimization NSGA prediction is compared with the response surface model combined with a genetic algorithm (RSM-GA) and we observed better productivity with NSGA.

scholarly journals Effects of Nitrogen Forms and Supply Mode on Lipid Production of Microalga Scenedesmus obliquus

Energies ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 697 ◽  
Author(s):  
Mei An ◽  
Li Gao ◽  
Wen Zhao ◽  
Weiguang Chen ◽  
Ming Li
Keyword(s):  
Nitrogen Concentration ◽  
Lipid Production ◽  
Scenedesmus Obliquus ◽  
Culture Conditions ◽  
Production Costs ◽  
Biodiesel Production ◽  
Nitrogen Forms ◽  
Microalgae Culture ◽  
New Process ◽  
Supply Mode

Optimization of the microalgae culture conditions could significantly reduce the production costs of microalgae-derived biodiesel. In the current study, a new process of adding different forms using the multiple small-dose method was employed. The effects of different forms of nitrogen (NaNO3, NH4Cl, and CH4N2O) and their concentrations (0.1, 0.5, 1, and 2 mg L−1) on the growth and lipid production of Scenedesmus obliquus were studied. Algae density and lipid production increased with increasing nitrogen concentration for all different forms of nitrogen except NH4Cl. The Scenedesmus obliquus growth was promoted by adding NaNO3 and CH4N2O, but was inhibited by adding NH4Cl. Adding 2 mg N L−1 of CH4N2O daily yielded the highest cell density (1.7 × 107 cells mL−1) and lipid production (242.4 mg L−1). These conditions can thus maintain the biomass of Scenedesmus obliquus, increase its lipid accumulation, and decrease the costs of biodiesel production.

Ultrastructure of the effect of T-514 obtained from Karwinskia humboldtiana on assembly and integrity of yeast's peroxisomes

1991 ◽  
Vol 49 ◽  
pp. 136-137
Author(s):  
J. Sepulveda-Saavedra ◽  
I. Vander-Klei ◽  
M. Venhuis ◽  
Y. Piñeyro-Lopez
Keyword(s):  
Culture Conditions ◽  
Toxic Effects ◽  
Candida Boidinii ◽  
Central Mexico ◽  
Poisonous Plant ◽  
Biological Behaviour ◽  
Enzyme Content ◽  
Fat Deposits ◽  
Yeast Model

Karwinskia humboldtiana is a poisonous plant that grows in semi desertic areas in north and central México. It produces several substances with different toxic effects. One of them designated T-514 damages severely the lung, kidney and liver, producing in the hepatoeyte large intracellular fat deposits and necrosis. Preliminary observations demonstrated that three is a decrease in the amount of peroxisomes in the hepatocytes of experimentally intoxicated rats and monkeys. To study the effect exerted by the T-514 on peroxisomes, a yeast model was selected, thus, three species: Saccha romices cerevisiae, Ilansenula polymorpha and Candida boidinii were used, because there is information concerning their peroxisome's morphology, enzyme content, biological behaviour under different culture conditions and biogenesis.

Scanning Microscopy of Human Intestinal Explants in Organ Culture

1972 ◽  
Vol 30 ◽  
pp. 396-397
Author(s):  
Bruce Wetzel ◽  
Robert Buscho ◽  
Raphael Dolin
Keyword(s):  
Electron Microscopy ◽  
Room Temperature ◽  
Culture Conditions ◽  
Human Fetuses ◽  
Enteric Disease ◽  
Organ Cultures ◽  
Growth Of A Variety ◽  
Normal Human ◽  
Fetal Intestine ◽  
Scanning Electron

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.

Lectin Binding Studies on RNA Tumor Virus-Infected Cells

1975 ◽  
Vol 33 ◽  
pp. 326-327
Author(s):  
D. C. Hixson
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Culture Conditions ◽  
3T3 Cells ◽  
Binding Studies ◽  
Con A ◽  
Microscopic Studies ◽  
Tumor Virus ◽  
Infected Cells ◽  
Cell Culture Conditions

The abilities of plant lectins to preferentially agglutinate malignant cells and to bind to specific monosaccharide or oligosaccharide sequences of glycoproteins and glycolipids make them a new and important biochemical probe for investigating alterations in plasma membrane structure which may result from malignant transformation. Electron and light microscopic studies have demonstrated clustered binding sites on surfaces of SV40-infected or tryp- sinized 3T3 cells when labeled with concanavalin A (con A). No clustering of con A binding sites was observed in normal 3T3 cells. It has been proposed that topological rearrangement of lectin binding sites into clusters enables con A to agglutinate SV40-infected or trypsinized 3T3 cells (1). However, observations by other investigators have not been consistent with this proposal (2) perhaps due to differences in reagents used, cell culture conditions, or labeling techniques. The present work was undertaken to study the lectin binding properties of normal and RNA tumor virus-infected cells and their associated viruses using lectins and ferritin-conjugated lectins of five different specificities.

Neutrophil migration through in vitro interstitial matrix

1992 ◽  
Vol 50 (1) ◽  
pp. 894-895
Author(s):  
J. Roemer ◽  
S.R. Simon
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cells ◽  
Inflammatory Cell ◽  
Neutrophil Migration ◽  
Culture Conditions ◽  
Aortic Smooth Muscle ◽  
Specific Culture

We are developing an in vitro interstitial extracellular matrix (ECM) system for study of inflammatory cell migration. Falcon brand Cyclopore membrane inserts of various pore sizes are used as a support substrate for production of ECM by R22 rat aortic smooth muscle cells. Under specific culture conditions these cells produce a highly insoluble matrix consisting of typical interstitial ECM components, i.e.: types I and III collagen, elastin, proteoglycans and fibronectin.

Ultrastructural Study of a differentiating human colon carcinoma cell line

1990 ◽  
Vol 48 (3) ◽  
pp. 208-209
Author(s):  
Sylvie Polak-Charcon ◽  
Mehrdad Hekmati ◽  
Yehuda Ben Shaul
Keyword(s):  
Cell Line ◽  
Morphological Changes ◽  
Secretory Granules ◽  
Human Colon ◽  
Cell Types ◽  
Culture Conditions ◽  
Morphological Evidence ◽  
Human Colon Carcinoma Cell ◽  
Colon Cell

The epithelium of normal human colon mucosa “in vivo” exhibits a gradual pattern of differentiation as undifferentiated stem cells from the base of the crypt of “lieberkuhn” rapidly divide, differentiate and migrate toward the free surface. The major differentiated cell type of the intestine observed are: absorptive cells displaying brush border, goblet cells containing mucous granules, Paneth and endocrine cells containing dense secretory granules. These different cell types are also found in the intestine of the 13-14 week old embryo.We present here morphological evidence showing that HT29, an adenocarcinoma of the human colon cell line, can differentiate into various cell types by changing the growth and culture conditions and mimic morphological changes found during development of the intestine in the human embryo.HT29 cells grown in tissue-culture dishes in DMEM and 10% FCS form at late confluence a multilayer of morphologically undifferentiated cell culture covered with irregular microvilli, and devoid of tight junctions (Figs 1-3).

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