Heat shock factors in carrot: genome-wide identification, classification, and expression profiles response to abiotic stress

2014 ◽  
Vol 42 (5) ◽  
pp. 893-905 ◽  
Author(s):  
Ying Huang ◽  
Meng-Yao Li ◽  
Feng Wang ◽  
Zhi-Sheng Xu ◽  
Wei Huang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Heat Shock ◽  
Expression Profiles ◽  
Heat Shock Factors ◽  
Genome Wide

scholarly journals Genome-Wide Identification and Functional Characterization of the Heat Shock Factor Family in Eggplant (Solanum melongena L.) under Abiotic Stress Conditions

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 915
Author(s):  
Jinglei Wang ◽  
Haijiao Hu ◽  
Wuhong Wang ◽  
Qingzhen Wei ◽  
Tianhua Hu ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Heat Shock ◽  
Environmental Stress ◽  
Expression Pattern ◽  
Expression Profiles ◽  
Solanum Melongena ◽  
Environmental Stresses ◽  
Stress Conditions ◽  
Heat Shock Factors ◽  
Genome Wide

Plant heat shock factors (Hsfs) play crucial roles in various environmental stress responses. Eggplant (Solanum melongena L.) is an agronomically important and thermophilic vegetable grown worldwide. Although the functions of Hsfs under environmental stress conditions have been characterized in the model plant Arabidopsis thaliana and tomato, their roles in responding to various stresses remain unclear in eggplant. Therefore, we characterized the eggplant SmeHsf family and surveyed expression profiles mediated by the SmeHsfs under various stress conditions. Here, using reported Hsfs from other species as queries to search SmeHsfs in the eggplant genome and confirming the typical conserved domains, we identified 20 SmeHsf genes. The SmeHsfs were further classified into 14 subgroups on the basis of their structure. Additionally, quantitative real-time PCR revealed that SmeHsfs responded to four stresses—cold, heat, salinity and drought—which indicated that SmeHsfs play crucial roles in improving tolerance to various abiotic stresses. The expression pattern of SmeHsfA6b exhibited the most immediate response to the various environmental stresses, except drought. The genome-wide identification and abiotic stress-responsive expression pattern analysis provide clues for further analysis of the roles and regulatory mechanism of SmeHsfs under environmental stresses.

scholarly journals Genome-wide survey of heat shock factors and heat shock protein 70s and their regulatory network under abiotic stresses in Brachypodium distachyon

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0180352 ◽  
Author(s):  
Feng Wen ◽  
Xiaozhu Wu ◽  
Tongjian Li ◽  
Mingliang Jia ◽  
Xinshen Liu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Regulatory Network ◽  
Abiotic Stresses ◽  
Brachypodium Distachyon ◽  
Heat Shock Factors ◽  
Genome Wide ◽  
Genome Wide Survey

scholarly journals HEAT SHOCK FACTOR BINDING PROTEIN limits meiotic crossovers by repressing HEI10 transcription

2021 ◽  
Author(s):  
Juhyun Kim ◽  
Jihye Park ◽  
Heejin Kim ◽  
Namil Son ◽  
Christophe Lambing ◽  
...  
Keyword(s):  
Heat Shock ◽  
Chromatin Immunoprecipitation ◽  
Response Regulator ◽  
Binding Protein ◽  
Temperature Response ◽  
Heat Shock Factor ◽  
Heat Shock Factors ◽  
Forward Genetic Screen ◽  
Factor Binding ◽  
Genome Wide

The number of meiotic crossovers is tightly controlled and most depend on pro-crossover ZMM proteins, such as the E3 ligase HEI10. Despite the importance of HEI10 dosage for crossover formation, how HEI10 transcription is controlled remains unexplored. In a forward genetic screen using a sensitive fluorescent seed crossover reporter in Arabidopsis thaliana we identify heat shock factor binding protein (HSBP) as a repressor of HEI10 transcription and crossover numbers. Using genome-wide crossover mapping and cytogenetics, we show that hsbp mutations or meiotic HSBP knockdowns increase ZMM-dependent crossovers towards the telomeres, mirroring the effects of HEI10 overexpression. Through RNA sequencing, DNA methylome and chromatin immunoprecipitation analysis, we reveal that HSBP directly represses HEI10 transcription by binding with heat shock factors (HSFs) at the HEI10 promoter and maintaining DNA methylation over the HEI10 5′ untranslated region. Our findings provide insights into how the temperature response regulator HSBP restricts meiotic HEI10 transcription and crossover number by attenuating HSF activity.

scholarly journals Wheat Heat Shock Factor TaHsfA6f Increases ABA Levels and Enhances Tolerance to Multiple Abiotic Stresses in Transgenic Plants

2020 ◽  
Vol 21 (9) ◽  
pp. 3121 ◽  
Author(s):  
Huihui Bi ◽  
Yue Zhao ◽  
Huanhuan Li ◽  
Wenxuan Liu
Keyword(s):  
Heat Shock ◽  
Abiotic Stresses ◽  
Expression Profiles ◽  
Abiotic Stress Tolerance ◽  
Gene Expression Profiles ◽  
Heat Shock Factors ◽  
Plant Tolerance ◽  
Sequencing Data ◽  
Aba Metabolism ◽  
Enhanced Sensitivity

Abiotic stresses are major constraints limiting crop growth and production. Heat shock factors (Hsfs) play significant roles in mediating plant resistance to various environmental stresses, including heat, drought and salinity. In this study, we explored the biological functions and underlying mechanisms of wheat TaHsfA6f in plant tolerance to various abiotic stresses. Gene expression profiles showed that TaHsfA6f has relatively high expression levels in wheat leaves at the reproductive stage. Transcript levels of TaHsfA6f were substantially up-regulated by heat, dehydration, salinity, low temperature, and multiple phytohormones, but was not induced by brassinosteroids (BR). Subcellular localization analyses revealed that TaHsfA6f is localized to the nucleus. Overexpression of the TaHsfA6f gene in Arabidopsis results in improved tolerance to heat, drought and salt stresses, enhanced sensitivity to exogenous abscisic acid (ABA), and increased accumulation of ABA. Furthermore, RNA-sequencing data demonstrated that TaHsfA6f functions through up-regulation of a number of genes involved in ABA metabolism and signaling, and other stress-associated genes. Collectively, these results provide evidence that TaHsfA6f participates in the regulation of multiple abiotic stresses, and that TaHsfA6f could serve as a valuable gene for genetic modification of crop abiotic stress tolerance.

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