A Deleted Deletion Site in a New Vector Strain and Exceptional Genomic Stability of Plaque-Purified Modified Vaccinia Ankara (MVA)

AbstractVectored vaccines based on highly attenuated modified vaccinia Ankara (MVA) are reported to be immunogenic, tolerant to pre-existing immunity, and able to accommodate and stably maintain very large transgenes. MVA is usually produced on primary chicken embryo fibroblasts, but production processes based on continuous cell lines emerge as increasingly robust and cost-effective alternatives. An isolate of a hitherto undescribed genotype was recovered by passage of a non-plaque-purified preparation of MVA in a continuous anatine suspension cell line (CR.pIX) in chemically defined medium. The novel isolate (MVA-CR19) replicated to higher infectious titers in the extracellular volume of suspension cultures and induced fewer syncytia in adherent cultures. We now extend previous studies with the investigation of the point mutations in structural genes of MVA-CR19 and describe an additional point mutation in a regulatory gene. We furthermore map and discuss an extensive rearrangement of the left telomer of MVA-CR19 that appears to have occurred by duplication of the right telomer. This event caused deletions and duplications of genes that may modulate immunologic properties of MVA-CR19 as a vaccine vector. Our characterizations also highlight the exceptional genetic stability of plaque-purified MVA: although the phenotype of MVA-CR19 appears to be advantageous for replication, we found that all genetic markers that differentiate wildtype and MVA-CR19 are stably maintained in passages of recombinant viruses based on either wildtype or MVA-CR.

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Analytical Study of Violence Theme in Victoria Schwab’s This Savage Song

University of Chitral Journal of Linguistics and Literature ◽

10.33195/jll.v3i1.110 ◽

2019 ◽

Keyword(s):

Analytical Study ◽

Research Paper ◽

The Novel ◽

Vicious Cycle ◽

Aggressive Approach ◽

Research Article ◽

The People ◽

The Social ◽

Different Dimensions ◽

The Right

This research article focuses on the theme of violence and its representation by the characters of the novel “This Savage Song” by Victoria Schwab. How violence is transmitted through genes to next generations and to what extent socio- psycho factors are involved in it, has also been discussed. Similarly, in what manner violent events and deeds by the parents affect the psychology of children and how it inculcates aggressive behaviour in their minds has been studied. What role is played by the parents in grooming the personality of children and ultimately their decisions to choose the right or wrong way has been argued. In the light of the theory of Judith Harris, this research paper highlights all the phenomena involved: How the social hierarchy controls the behaviour. In addition, the aggressive approach of the people in their lives has been analyzed in the light of the study of second theorist Thomas W Blume. As the novel is a unique representation of supernatural characters, the monsters, which are the products of some cruel deeds, this research paper brings out different dimensions of human sufferings with respect to these supernatural beings. Moreover, the researcher also discusses that, in what manner the curse of violence creates an inevitable vicious cycle of cruel monsters that makes the life of the characters turbulent and miserable.

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The Right to Dream: Gender, Modernity, and the Problem of Class in Kate O'Brien's Bourgeois Bildungsromane

Irish University Review ◽

10.3366/iur.2019.0406 ◽

2019 ◽

Vol 49 (2) ◽

pp. 276-289

Author(s):

Naoise Murphy

Keyword(s):

Middle Class ◽

Identity Formation ◽

Coming Of Age ◽

Female Identity ◽

Gender And Sexuality ◽

The Novel ◽

Class Status ◽

The Right ◽

Aristocratic Culture ◽

Modern Form

Feminist critics have celebrated Kate O'Brien's pioneering approach to gender and sexuality, yet there has been little exploration of her innovations of the coming-of-age narrative. Creating a modern Irish reworking of the Bildungsroman, O'Brien's heroines represent an idealized model of female identity-formation which stands in sharp contrast to the nationalist state's vision of Irish womanhood. Using Franco Moretti's theory of the Bildungsroman, a framing of the genre as a thoroughly ‘modern’ form of the novel, this article applies a critical Marxist lens to O'Brien's output. This reading brings to light the ways in which the limitations of the Bildungsroman work to constrain O'Brien's subversive politics. Their middle-class status remains an integral part of the identity of her heroines, informing the forms of liberation they seek. Fundamentally, O'Brien's idealization of aristocratic culture, elitist exceptionalism and ‘detachment of spirit’ restricts the emancipatory potential of her vision of Irish womanhood.

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Homosexuality in K. Sello Duiker’s The Quiet Violence of Dreams: Demythologising the Imaginings and Universalising the Reality

Imbizo ◽

10.25159/2663-6565/6838 ◽

2020 ◽

Vol 11 (2) ◽

Author(s):

Adetunji Kazeem Adebiyi-Adelabu

Keyword(s):

Coming Out ◽

The Novel ◽

Same Sex ◽

Black African ◽

Sex Preference ◽

National Allegory ◽

Critical Attention ◽

Sex Orientation ◽

The Right

Sello Duiker’s The Quiet Violence of Dreams offers an extensive treatment of homosexuality, a preoccupation which, until recently, is rare in black African fiction. On this account, as well as its depth and openness, the work has attracted some critical attention. It has been read from a masculinity perspective, as a coming-out novel, as a national allegory, as a work that challenges the notion of fixed sexuality, as a work that normalises same-sex sexuality, and so forth. Unlike these studies, this article examines the representation and disquisition around same-sex preference in the novel, with a view to demonstrating how some myths about homosexuality are exploded in the groundbreaking work, and showing that the narrative could also be apprehended as intellectual advocacy for the right to same-sex orientation.

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Measuring Earwax Cortisol Concentration Using a Non-Stressful Method (Preprint)

10.2196/preprints.19383 ◽

2020 ◽

Author(s):

Andrés Herane-Vives

Keyword(s):

Cortisol Level ◽

Local Stress ◽

Extraction Methods ◽

Cortisol Concentration ◽

The Novel ◽

Sampling Device ◽

Related Factors ◽

Novel Device ◽

Cortisol Levels ◽

The Right

BACKGROUND “Short-term” samples are not the most appropriate for reflecting Chronic Cortisol Concentration (CCC). Although hair is used for reflecting the systemic cortisol level over “long-term”, its use appears clinically problematic. Local stress and non-stress related factors may release a circumscribed cortisol secretion that is accumulated in hair. Non-stressful earwax extraction methods may provide a more accurate specimen to measure CCC. OBJECTIVE Correlate cortisol levels using hair, serum and earwax samples METHODS Earwax from both ears of 37 controls were extracted using a clinical procedure commonly associated with local pain. One month later, earwax from the left ear side was extracted using the same procedure, and earwax from the right ear side was comfortably extracted, using an earwax self-sampling device. Participants also provided one centimetre of hair that represented the retrospective month of cortisol output, and one serum sample that reflected the effect of systemic stressors on cortisol levels. Earwax (ECC), Hair (HCC) and Serum (SCC) Cortisol Concentration were correlated and compared. Confounders´ effect on cortisol levels were studied. RESULTS Serum showed the largest and hair the lowest cortisol concentration (p<0.01). Left-ECC was larger than Right-ECC (p=0.03). Right-ECC was the only sample unaffected by confounders (all p>0.05). Right-ECC and HCC showed the only significant association (r=0.39; p=0.03). CONCLUSIONS The self-sampling device did not represent a local stressor for the ceruminous glands. It provided the cortisol level with the least likely to be affected by confounding factors over the previous month. ECC using the novel device may constitute another accurate, but more suitable and affordable specimen for measuring CCC.

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The sharp bounds of the second and third Hankel determinants for the class 𝓢𝓛*

Mathematica Slovaca ◽

10.1515/ms-2017-0398 ◽

2020 ◽

Vol 70 (4) ◽

pp. 849-862

Author(s):

Shagun Banga ◽

S. Sivaprasad Kumar

Keyword(s):

Triangle Inequality ◽

Starlike Functions ◽

Sharp Bound ◽

The Novel ◽

Sharp Bounds ◽

Hankel Determinants ◽

Lemniscate Of Bernoulli ◽

Carathéodory Functions ◽

The Right

AbstractIn this paper, we use the novel idea of incorporating the recently derived formula for the fourth coefficient of Carathéodory functions, in place of the routine triangle inequality to achieve the sharp bounds of the Hankel determinants H3(1) and H2(3) for the well known class 𝓢𝓛* of starlike functions associated with the right lemniscate of Bernoulli. Apart from that the sharp bound of the Zalcman functional: $\begin{array}{} |a_3^2-a_5| \end{array}$ for the class 𝓢𝓛* is also estimated. Further, a couple of interesting results of 𝓢𝓛* are also discussed.

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Measuring myocardial extracellular volume of the right ventricle in patients with congenital heart disease

Scientific Reports ◽

10.1038/s41598-021-81440-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Nadya Al-Wakeel-Marquard ◽

Tiago Ferreira da Silva ◽

Sarah Jeuthe ◽

Sanaz Rastin ◽

Frédéric Muench ◽

...

Keyword(s):

Congenital Heart Disease ◽

Heart Disease ◽

Image Quality ◽

Right Ventricle ◽

Wall Thickness ◽

Congenital Heart ◽

Extracellular Volume ◽

Analysis Tool ◽

Custom Made ◽

The Right

AbstractThe right ventricle´s (RV) characteristics—thin walls and trabeculation—make it challenging to evaluate extracellular volume (ECV). We aimed to assess the feasibility of RV ECV measurements in congenital heart disease (CHD), and to introduce a novel ECV analysis tool. Patients (n = 39) and healthy controls (n = 17) underwent cardiovascular magnetic resonance T1 mapping in midventricular short axis (SAX) and transverse orientation (TRANS). Regions of interest (ROIs) were evaluated with regard to image quality and maximum RV wall thickness per ROI in pixels. ECV from plane ROIs was compared with values obtained with a custom-made tool that derives the mean T1 values from a “line of interest” (LOI) centered in the RV wall. In CHD, average image quality was good (no artifacts in the RV, good contrast between blood/myocardium), and RV wall thickness was 1–2 pixels. RV ECV was not quantifiable in 4/39 patients due to insufficient contrast or wall thickness < 1 pixel. RV myocardium tended to be more clearly delineated in SAX than TRANS. ECV from ROIs and corresponding LOIs correlated strongly in both directions (SAX/TRANS: r = 0.97/0.87, p < 0.001, respectively). In conclusion, RV ECV can be assessed if image quality allows sufficient distinction between myocardium and blood, and RV wall thickness per ROI is ≥ 1 pixel. T1 maps in SAX are recommended for RV ECV analysis. LOI application simplifies RV ECV measurements.

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ERP benefits capability framework: orchestration theory perspective

Business Process Management Journal ◽

10.1108/bpmj-11-2015-0162 ◽

2018 ◽

Vol 24 (1) ◽

pp. 266-294 ◽

Cited By ~ 6

Author(s):

Amgad Badewi ◽

Essam Shehab ◽

Jing Zeng ◽

Mostafa Mohamad

Keyword(s):

Cost Effective ◽

Organizational Capabilities ◽

Erp Implementation ◽

Content Type ◽

Quantitative Studies ◽

Financial Perspective ◽

Complementary Resources ◽

Benefits Realization ◽

Research Questions ◽

The Right

Purpose The purpose of this paper is to answer two research questions: what are the ERP resources and organizational complementary resources (OCRs) required to achieve each group of benefits? And on the basis of its resources, when should an organization invest more in ERP resources and/or OCRs so that the potential value of its ERP is realised? Design/methodology/approach Studying 12 organizations in different countries and validating the results with 8 consultants. Findings ERP benefits realization capability framework is developed; it shows that each group of benefits requires ERP resources (classified into features, attached technologies and information technology department competences) and OCRs (classified into practices, attitudes, culture, skills and organizational characteristics) and that leaping ahead to gain innovation benefits before being mature enough in realising a firm’s planning and automation capabilities could be a waste of time and effort. Research limitations/implications It is qualitative study. It needs to be backed by quantitative studies to test the results. Practical implications Although the “P” in ERP stands for planning, many academics and practitioners still believe that ERP applies to automation only. This research spotlights that the ability to invest in ERP can increase the innovation and planning capabilities of the organization only if it is extended and grown at the right time and if it is supported by OCRs. It is not cost effective to push an organization to achieve all the benefits at the same time; rather, it is clear that an organization would not be able to enjoy a higher level of benefits until it achieves a significant number of lower-level benefits. Thus, investing in higher-level benefit assets directly after an ERP implementation, when there are no organizational capabilities available to use these assets, could be inefficient. Moreover, it could be stressful to users when they see plenty of new ERP resources without the ability to use them. Although it could be of slight benefit to introduce, for example, business intelligence to employees in the “stabilizing period” (Badewi et al., 2013), from the financial perspective, it is a waste of money since the benefits would not be realised as expected. Therefore, orchestrating ERP assets with the development of organizational capabilities is important for achieving the greatest effectiveness and efficiency of the resources available to the organization. This research can be used as a benchmark for designing the various blueprints required to achieve different groups of benefits from ERP investments. Originality/value This research addresses two novel questions: RQ1: what are the ERP resources and OCRs required to achieve the different kinds of ERP benefits? RQ2: when, and on what basis, should an organization deploy more resources to leverage the ERP business value?

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CRITICAL REVIEW OF EMULSION STABILITY AND CHARACTERIZATION TECHNIQUES IN OIL PROCESSING

Journal of Energy Resources Technology ◽

10.1115/1.4051571 ◽

2021 ◽

pp. 1-36

Author(s):

Vahideh Angardi ◽

Ali Ettehadi ◽

Özgün Yücel

Keyword(s):

Separation Efficiency ◽

Petroleum Industry ◽

Cost Effective ◽

Downstream Processing ◽

Physical Factors ◽

Comprehensive Review ◽

Factors Affecting ◽

Mathematical Explanations ◽

The Stability ◽

The Right

Abstract Effective separation of water and oil dispersions is considered a critical step in the determination of technical and economic success in the petroleum industry over the years. Moreover, a deeper understanding of the emulsification process and different affected parameters is essential for cost-effective oil production, transportation, and downstream processing. Numerous studies conducted on the concept of dispersion characterization indicate the importance of this concept, which deserves attention by the scientific community. Therefore, a comprehensive review study with critical analysis on significant concepts will help readers follow them easily. This study is a comprehensive review of the concept of dispersion characterization and conducted studies recently published. The main purposes of this review are to 1) Highlight flaws, 2) Outline gaps and weaknesses, 3) Address conflicts, 4) Prevent duplication of effort, 5) List factors affecting dispersion. It was found that the separation efficiency and stability of dispersions are affected by different chemical and physical factors. Factors affecting the stability of the emulsions have been studied in detail and will help to look for the right action to ensure stable emulsions. In addition, methods of ensuring stability, especially coalescence are highlighted, and coalescence mathematical explanations of phenomena are presented.

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Improvement of the Impact Properties of Composite Laminates by Means of Nano-Modification of the Matrix—A Review

Applied Sciences ◽

10.3390/app8122406 ◽

2018 ◽

Vol 8 (12) ◽

pp. 2406 ◽

Cited By ~ 20

Author(s):

Hamed Saghafi ◽

Mohamad Fotouhi ◽

Giangiacomo Minak

Keyword(s):

Composite Laminates ◽

Cost Effective ◽

Careful Consideration ◽

Impact Performance ◽

Compression After Impact ◽

The Matrix ◽

The Right ◽

2D And 3D ◽

The Impact ◽

Selection Of

This paper reviews recent works on the application of nanofibers and nanoparticle reinforcements to enhance the interlaminar fracture toughness, to reduce the impact induced damage and to improve the compression after impact performance of fiber reinforced composites with brittle thermosetting resins. The nanofibers have been mainly used as mats embedded between plies of laminated composites, whereas the nanoparticles have been used in 0D, 1D, 2D, and 3D dimensional patterns to reinforce the matrix and consequently the composite. The reinforcement mechanisms are presented, and a comparison is done between the different papers in the literature. This review shows that in order to have an efficient reinforcement effect, careful consideration is required in the manufacturing, materials selection and reinforcement content and percentage. The selection of the right parameters can provide a tough and impact resistant composite with cost effective reinforcements.

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A New Fluorescence Probe for Rapid Detection of Amyloid

10.26434/chemrxiv.6982895.v1 ◽

2018 ◽

Author(s):

Nidhi Gour ◽

Vivek Shinh Kshtriya ◽

Bharti Koshti ◽

Shradhey Gupta ◽

khashti Ballabh Joshi ◽

...

Keyword(s):

Rapid Detection ◽

Control Experiment ◽

Fluorescence Probe ◽

Photophysical Properties ◽

Cost Effective ◽

The Novel ◽

High Quantum Yield ◽

Turn On ◽

Heat Treated ◽

Amyloidogenic Peptides

We report efficient synthesis and photophysical properties of a novel fluorescent pyridothiazole based construct by a facile methodology and its application as a sensor for rapid, selective as well as sensitive detection of amyloid fibres. The novel compound has exceptionally high quantum yield and sensitivity. The fluorescence of pyridothiazole construct is completely quenched (Turn-OFF) upon addition of cupric ions and the fluorescence is recovered on addition of amyloidogenic peptides like Aβ42 (Turn-ON). The control experiment with native and heat treated BSA suggest that the new probe binds to beeta/β sheets present in amyloid. The new probe offers direct visualization of fluorescence ‘Turn-OFF’ ‘Turn-ON’ under long UV without the use of sophisticated instrumentation. Hence, a very simple, facile and cost-effective methodology for the rapid detection of amyloid is presented and it may in all possibility pave the way for the design of a better probe for sensing amyloids than conventional ThT.

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