scholarly journals Phenolic composition of leaf and flower extracts of black cherry (Prunus serotina Ehrh.)

2021 ◽  
Vol 78 (3) ◽  
Author(s):  
Jakub Brozdowski ◽  
Boguslawa Waliszewska ◽  
Sasa Gacnik ◽  
Metka Hudina ◽  
Robert Veberic ◽  
...  

Abstract Key Message The best yields in the extraction of hydroxycinnamic acids, flavanols and total phenolics from black cherry flowers were obtained with 40% ethanol and the poorest with water. A 30% higher content of total phenolics was obtained from leaves by using methanol rather than water. Context Black cherry (Prunus serotina Erhr.) is widespread in Europe. Because it is an invasive species, most research has been aimed at trying to find a method to remove this shrub from forests. The incentive for the development of the research was that we were trying to find a possible folk use of black cherry leaves and flowers. Aims The aim of this work was to study the detailed phenolic profile of extracts of P. serotina leaves and flowers. Methods Three types of extracts of P. serotina were made. The extracts were analysed for differences in phenolics content based on different extraction methods. HPLC-MSn was used to identify the phenolic compounds, and HPLC–DAD was used for their quantification. Results The results show that different extraction methods result in differences in the amount of extractables. Flavanols are the main group of identified compounds in both leaves and flowers. A larger extraction efficiency leads to a higher amount of phenolics in the flowers compared to leaves (49.8 vs. 36.5 g/kg dry weight). No difference was detected in the amount of phenolics between water extracts of leaves and flowers. Each extract can be considered rich in phenolics. Conclusion This work shows that leaves and flowers of P. serotina are a rich source of phenolic compounds. We provide interesting results that might be of interest in the industrial valuation of several compounds.

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Wojciech Koch ◽  
Tomasz Baj ◽  
Wirginia Kukula-Koch ◽  
Zbigniew Marzec

AbstractThe determination of phenolic acids’ and flavonoids’ content in daily food rations (DFRs) of a selected group of male and female students and the development of chromatographic conditions is the primary goal of the study. The presence of 7 phenolic components were confirmed in the prepared extracts from all diets reconstructed within a period of 3 years. The highest concentrations were determined for hesperidin (124 and 55.6 mg for women and men, respectively), naringin (47.6 mg in female and 37 mg in male diets) and chlorogenic acid (19.7 and 19.8 mg for women and men). The antioxidant potential of the daily food rations, measured with a DPPH test, was higher for women (range 47.1‒78.8%) than for men (range 34.5‒78.0%) and was found to strongly correlate with the total phenolics content of the samples (Folin-Ciocalteu test) (correlation coefficient 0.90).


Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 298 ◽  
Author(s):  
Aldo Tava ◽  
Łukasz Pecio ◽  
Roberto Lo Scalzo ◽  
Anna Stochmal ◽  
Luciano Pecetti

Phenolics are important mediators in plant-environment interactions. The presence and concentration of phenolic compounds and their antioxidant activity were evaluated in leaves and flowers of a set of Trifolium species originating from contrasting environments encompassing lowland and mountain sites. The current germplasm proved a great reservoir of phenolic compounds, with different chemical structure and, possibly, diversified biological activity. Germplasm groups with specific phenolic composition were observed. In some cases, different patterns bore a taxonomic meaning. Lowland germplasm showed higher concentration of total phenolics in leaves than mountain accessions (50.30 vs. 34.19 mg/g dry matter (DM)), while the latter had higher concentration in flowers (114.16 vs. 57.44 mg/g DM). Outstanding concentration of isoflavones was observed in leaves of lowland germplasm (24.19 mg/g DM), and of both proanthocyanidins and flavonoids in flowers of mountain germplasm (53.81 and 56.62 mg/g DM, respectively). The pattern of phenolic composition in lowland and mountain germplasm was suggestive of different adaptive strategies. Three assays of antioxidant activity were tested, which were characterised by rather different reactivity towards phenolic composition. The scavenging activity was higher for leaf extracts of lowland germplasm, and for flower extracts of mountain germplasm. Besides identifying germplasm of interest, this study also suggested possible links between environmental factors and concentration and composition of phenolic compounds.


2017 ◽  
Vol 23 (1) ◽  
pp. 39-47
Author(s):  
Aleksandra Misan ◽  
Bojana Saric ◽  
Ivan Milovanovic ◽  
Pavle Jovanov ◽  
Ivana Sedej ◽  
...  

Due to a high content of rutin (2-10%), dried buckwheat leaf and flower (DBLF) formulations were shown to be efficient in the treatment of vascular diseases. In order to find a cost effective way for the extraction of antioxidants, the effects of ethanol/water ratio and temperature on the extraction efficiency of phenolic compounds and the mechanisms of antioxidant action of the extracts were tested. Extraction with ethanol/water mixture (80:20, v/v) for 24 h at room temperature, after the mixture was just brought to boil was demonstrated to be an efficient and cheap way for obtaining a high yield of rutin (49.94?0.623 mg/g DBLF). The most abundant phenolic compounds in DBLF extracts were rutin and chlorogenic acid. Flavonoids, especially rutin, were shown to be the most responsible for the antioxidant activity in all investigated lipid model systems, acting as free radical scavengers, electron-donating substances and chelators of iron ions. In ?-carotene bleaching tests, the extracts with the highest activity were as efficient as BHT (butylated hydroxytoluole). Regarding the results of antihemolytic and Schaal oven tests, the extracts demonstrated remarkable ability to inhibit the oxidative destruction of erythrocytes and to prolong the beginning of the oxidation process in sunflower oil.


Author(s):  
Nino Gamkrelidze ◽  
◽  
Giorgi Kvartskava ◽  

Plant extracts used in food production significantly improve the quality of food. Chemical composition is the main prerequisites for the final use of plant extracts. The chemical composition of the extracts is affected by the extraction method and conditions - temperature, duration and solvent. On the basis of experimental data optimal conditions for the extraction of phenolic compounds from the fruits and leaves of blackthorn (Prunus spinosa) were selected. Among the extraction methods used in the experiment, the highest content of phenolic compounds in obtained extracts was observed during the ultrasonic extraction. A mixture of 0.25 parts of water and 0.75 parts of 96% ethanol showed best results from different volume ratios of water and ethanol. The optimal extraction time was 90 minutes. And the optimum temperature is 45°C. The total content of phenolic compounds in the extracts obtained by the abovementioned method amounted is 35.62±0.17 mgg -1(dry weight) for blackthorn fruits and 44,76 ± 0.08mgg -1(dry weight) for the leaves.


Author(s):  
Rasheeda Khanam ◽  
R. Gyana Prasuna

The present article identifies the best solvent for extracting phenolic compounds from the dairy waste, i.e., ghee residue, by using various polar and non-polar solvents in extraction methods (Soxhlet and liquid-liquid extraction methods).


Forests ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 762
Author(s):  
Jakub Brozdowski ◽  
Boguslawa Waliszewska ◽  
Jakub Loffler ◽  
Metka Hudina ◽  
Robert Veberic ◽  
...  

The forest understory is rich in fruit-bearing trees and shrubs. They include both native species and invasive alien plant species (IAPS). One of the most spreading IAPS is black cherry (Prunus serotina E.). Problems with the invasiveness of P. serotina is well known in many European countries. However, there are very few studies related to this IAPS. This article aims to fill the gap in research on the bioactive compounds of black cherry fruits and thus to start a discussion on the potential use of this species on an industrial or semi-industrial scale, which may lead to the reduction of this species presence in forests. Fruits were collected in a forest of Northern Poland. Contents of sugars, phenolic compounds as well as cyanogenic glycosides were determined. Pphenolic compounds and cyanogenic glycoside were assayed using HPLC-DAD coupled with MSn. Sugars and organic acids were determined with the use of HPLC-DAD. Fruits of P. serotina can be considered as rich in anthocyanins, even at a lower level of total phenolics than in their plantation-grown couterparts, fruits of black cherry are still a good source of dietary phenolics of natural, forest-grown origin. When stones are not crushed during processing the level of GCC is within a safe, admissible range.


2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Fahad Mohammed Al-Jasass ◽  
Muhammad Siddiq ◽  
Dalbir S. Sogi

Dates (Phoenix dactylifera L.) are nutrient-rich fruit consumed throughout the world, either directly or in several food products. Six commercially available date cultivars in the US were analyzed for total phenolics, antioxidant activity using ABTS, DPPH, FRAP, and ORAC assays, and instrumental color. Total phenolics content varied from 33 to 125 mg GAE/100 g dry weight, with the highest in Barni (Saudi Arabia). Antioxidant values as determined by the ABTS in Deglet Nour (Algeria), Deglet Nour (California), Deglet Noor (Tunisia), Shahia (Tunisia), Barni (Saudi Arabia), and Khudri (Saudi Arabia) were 1300, 1047, 796, 452, 776, and 341 μmol TE/g dry weight, respectively. Antioxidative properties as measure by DPPH, FRAP, and ORAC varied from 3.27 to 3.54, 3.29 to 5.22, and 189 to 243 μmol TE/g dry basis, respectively. Fruit and pulp color of Deglet Nour (Algeria) was lighter whereas pulp of Barni (Saudi Arabia) was the darkest. Antioxidant values varied with different techniques used and also followed a different pattern than that of phenolics content.


Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1649 ◽  
Author(s):  
Jaime A. Arboleda Mejia ◽  
Arianna Ricci ◽  
Ana S. Figueiredo ◽  
Andrea Versari ◽  
Alfredo Cassano ◽  
...  

The winemaking process generates a large amount of residues such as vine shots, stalks, grape pomace, and wine lees, which were only recently considered for exploitation of their valuable compounds. The purpose of this work was to investigate the performance of nanofiltration for the recovery of phenolic compounds, with bioactive capacity like antioxidant, from red grape pomace extract. Four membranes were compared in this study—three cellulose acetate (CA series: lab-prepared by phase inversion) and one commercial (NF90). All membranes were characterized for their hydraulic permeability and rejection coefficients to reference solutes like saccharose, glucose, raffinose, polyethylene glycol, sodium chloride, and sodium sulfate. Permeation flowrates and rejection coefficients towards total phenolics content, antioxidant activity, proanthocyanidins, glucose and fructose were measured in the nanofiltration of grape pomace extract using selected operating conditions. Among the investigated membranes, the CA400-22 exhibited the highest permeate flux (50.58 L/m2 h at 20 bar and 25 °C), low fouling index (of about 23%), the lowest rejection coefficients towards the reference solutes and the best performance in terms of separation between sugars and phenolic compounds. Indeed, the observed rejections for glucose and fructose were 19% and 12%, respectively. On the other hand, total phenolics content and proanthocyanidins were rejected for 73% and 92%, respectively.


Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 120 ◽  
Author(s):  
Susana Río Segade ◽  
Maria Alessandra Paissoni ◽  
Mar Vilanova ◽  
Vincenzo Gerbi ◽  
Luca Rolle ◽  
...  

Plant proteins have been proposed as an alternative to animal-origin proteins in the wine industry because they are allergen-free and vegan-friendly. The aim of this study was to evaluate the effectiveness of plant proteins as fining agents on red wines with different phenolic composition. Two formulations for commercially available vegetal proteins (potato and pea origin) were assessed at two doses to modulate the fining treatment to the wine phenolic profile. The results evidenced that fining agents derived from plants have different levels of effectiveness on the removal of phenolic compounds depending on the origin, the formulation used, dose applied, and also wine characteristics. On Nebbiolo wine, the study was particularly significant due to its phenolic composition. One pea-based fining agent had an effect comparable to gelatin (animal origin) on the removal of polymeric flavanols with a minor loss of anthocyanins and therefore better preserving the wine color in terms of intensity and hue. For Primitivo, Montepulciano, and Syrah wines, even though there was a formulation-dependent effect, vegetal proteins gave more balanced reductions in terms of target phenolic compounds contributing to astringency and color perception.


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