Oral administration of betaine ameliorates ethanol-induced gastric injury in rats through its antioxidant effects

Abstract Background Liver dysfunction is a predominant complication of diabetes. Herbal remedies such as garlic are commonly used for reducing diabetic complications. In this study the effect of garlic extract on glucose level, liver enzymes activities in the serum and nitric oxide (NO) level, oxidative stress status, and histology in the liver tissues of streptozotocin-induced diabetes (type 1) was investigated. Methods Twenty-four adult male Wistar rats were randomized and divided into four groups: control rats, diabetic rats, diabetic rats treated with garlic, and garlic-treated normal rats. Glucose level and liver enzymes activities were determined by colorimetric assay in the serum. NO levels by Griess method, oxidative stress parameters including malondialdehyde (MDA), total oxidative status (TOS), and total antioxidant capacity (TAC) by spectrophotometric method, and histopathological examination by hematoxylin and eosin staining method were evaluated in the liver tissues. Results Glucose level, liver enzymes activities, MDA, TOS, and NO levels were increased and TAC level decreased significantly in diabetic rats in comparison with control rats (p<0.01); whereas, after oral administration of garlic, glucose level, liver enzymes activities, MDA, TOS, and NO levels were decreased and TAC level increased significantly near to the normal levels (p<0.05). Conclusion The results showed the hypoglycemic and antioxidant effects of garlic in the livers of type 1 diabetic rats.

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Phycocyanin and phycocyanobilin fromSpirulina platensisprotect against diabetic nephropathy by inhibiting oxidative stress

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00648.2011 ◽

2013 ◽

Vol 304 (2) ◽

pp. R110-R120 ◽

Cited By ~ 70

Author(s):

Jing Zheng ◽

Toyoshi Inoguchi ◽

Shuji Sasaki ◽

Yasutaka Maeda ◽

Mark F. McCarty ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetic Nephropathy ◽

Oral Administration ◽

Mesangial Cells ◽

Vascular Complications ◽

Blue Green Algae ◽

Beneficial Effects ◽

Diabetic Vascular Complications ◽

Tumor Growth Factor ◽

Antioxidant Effects

We and other investigators have reported that bilirubin and its precursor biliverdin may have beneficial effects on diabetic vascular complications, including nephropathy, via its antioxidant effects. Here, we investigated whether phycocyanin derived from Spirulina platensis, a blue-green algae, and its chromophore phycocyanobilin, which has a chemical structure similar to that of biliverdin, protect against oxidative stress and renal dysfunction in db/db mice, a rodent model for Type 2 diabetes. Oral administration of phycocyanin (300 mg/kg) for 10 wk protected against albuminuria and renal mesangial expansion in db/db mice, and normalized tumor growth factor-β and fibronectin expression. Phycocyanin also normalized urinary and renal oxidative stress markers and the expression of NAD(P)H oxidase components. Similar antioxidant effects were observed following oral administration of phycocyanobilin (15 mg/kg) for 2 wk. Phycocyanobilin, bilirubin, and biliverdin also inhibited NADPH dependent superoxide production in cultured renal mesangial cells. In conclusion, oral administration of phycocyanin and phycocyanobilin may offer a novel and feasible therapeutic approach for preventing diabetic nephropathy.

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Syzygium aromaticum water extract attenuates ethanol-induced gastric injury through antioxidant effects in rats

Molecular Medicine Reports ◽

10.3892/mmr.2016.5269 ◽

2016 ◽

Vol 14 (1) ◽

pp. 361-366 ◽

Cited By ~ 3

Author(s):

SEONG EUN JIN ◽

MEE-YOUNG LEE ◽

IN-SIK SHIN ◽

WOO-YOUNG JEON ◽

HYEKYUNG HA

Keyword(s):

Water Extract ◽

Gastric Injury ◽

Syzygium Aromaticum ◽

Antioxidant Effects

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Protective Effects of the Traditional Herbal Formula Oryeongsan Water Extract on Ethanol-Induced Acute Gastric Mucosal Injury in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/438191 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Woo-Young Jeon ◽

Mee-Young Lee ◽

In-Sik Shin ◽

Hye-Sun Lim ◽

Hyeun-Kyoo Shin

Keyword(s):

Antioxidant Enzymes ◽

Gastric Mucosa ◽

Acute Toxicity ◽

Oral Administration ◽

Protective Effect ◽

Water Extract ◽

Mucosal Injury ◽

Toxicity Study ◽

Gastric Injury ◽

Acute Toxicity Study

This study was performed to evaluate the protective effect and safety of Oryeongsan water extract (OSWE) on ethanol-induced acute gastric mucosal injury and an acute toxicity study in rats. Acute gastric lesions were induced via intragastric oral administration of absolute ethanol at a dose of 5 mL/kg. OSWE (100 and 200 mg/kg) was administered to rats 2 h prior to the oral administration of absolute ethanol. The stomach of animal models was opened and gastric mucosal lesions were examined. Gastric mucosal injuries were evaluated by measuring the levels of malondialdehyde (MDA), glutathione (GSH), and the activity of antioxidant enzymes. In the acute toxicity study, no adverse effects of OSWE were observed at doses up to 2000 mg/kg/day. Administration of OSWE reduced the damage by conditioning the gastric mucosa against ethanol-induced acute gastric injury, which included hemorrhage, hyperemia, and loss of epithelial cells. The level of MDA was reduced in OSWE-treated groups compared with the ethanol-induced group. Moreover, the level of GSH and the activity of antioxidant enzymes were significantly increased in the OSWE-treated groups. Our findings suggest that OSWE has a protective effect on the gastric mucosa against ethanol-induced acute gastric injury via the upregulation of antioxidant enzymes.

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Hypoglycemic, Hypolipidemic, Renal Protective and Antioxidant Activity of Annona muricata in Streptozotocin-Induced Diabetic Rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.01121 ◽

2021 ◽

pp. 6484-6490

Author(s):

Rasha N. Mohammed ◽

Hanan H. Ramadhan ◽

Falah H. Shari

Keyword(s):

Oral Administration ◽

Aqueous Extract ◽

Diabetic Control ◽

Diabetic Rats ◽

Control Group ◽

Albino Rats ◽

Aqueous Extracts ◽

Annona Muricata ◽

Diabetic Control Group ◽

Antioxidant Effects

Annona muricata, an herbal plant commonly used in traditional medicine to manage numerous diseases, diabetes as other diseases could be managed with herbal medicine. This study was designed to be investigated the antidiabetic, hypolipidemic, renal protective, and antioxidant effects of aqueous extracts of Annona muricata as used alone or combined with metformin in streptozotocin (STZ)-induced diabetic rats. Methods: the study was involved twenty adult Wister albino rats in four groups (five rats in each) and designated as groups, control group (1), and experimental groups (2, 3, 4). Diabetes was induced in experimental groups by 60 mg/kg intravenous streptozotocin injection. Group 2: serves as a diabetic control group, Group 3: diabetic rats treated with oral administration of 100 mg/kg of Annona muricata aqueous extract, Group 4: diabetic rats treated with combination (100 mg/kg aqueous extract of Annona muricata + 50 mg/kg metformin). The treatment continuous daily for 4 weeks to determine the levels of blood glucose and biochemical analysis. Result: aqueous extract of Annona muricata was reduced the serum glucose level effectively in streptozotocin-induced diabetic rats, by 48% and 55% after 28 consecutive days of treatment when used alone and with metformin, respectively. These compared to the preliminary values and the reduction was statistically significant compared to a diabetic control group. Daily oral administration of 100 mg/kg aqueous extract of Annona muricata for 4 weeks to streptozotocin-induced diabetic rats significantly reduced the level of total cholesterol, urea, creatinine, and MDA, whereas the reduction was non-significant in triglyceride and VLDL-cholesterol levels as compared to the non-treated diabetic group. However, the reduction is more significant in streptozotocin-induced diabetes rats that were treated with a combination of Annona muricata and metformin when compared to the diabetic control group. Conclusion: Aqueous extracts of Annona muricata have anti-diabetic action through their hypoglycemic, hypolipidemic, renal protective, and antioxidant effects in streptozotocin-induced diabetic rats. Thus, can be used alone or with anti-hyperglycemic drugs as metformin in the management of DM. The combination is preferred in severe hyperglycemic cases with more hypoglycemic effect requirements.

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Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6584945 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Jun Zhang ◽

Zai-Lin Fu ◽

Zhao-Xing Chu ◽

Bi-Wei Song

Keyword(s):

Superoxide Dismutase ◽

Gastric Ulcer ◽

Medicinal Plant ◽

Oral Administration ◽

Protein Content ◽

Gastric Injury ◽

Gastric Tissue ◽

Gastroprotective Activity ◽

Ulcer Index ◽

Antiulcer Drug

Background. Abelmoschus manihot (L.) Medic flower is a medicinal plant for the treatment of diseases in China. The present study was carried out to scientifically validate the gastroprotective activity and clarify the possible mechanism of the total flavones from Abelmoschus manihot (L.) Medic flowers (TFA). Methods. Gastric ulcer was induced in mice by oral administration of ethanol. The gastroprotective activity of TFA was evaluated by the gastric ulcer index and histological examinations. The gastric tissue was collected in the form of homogenate. The level of malondialdehyde (MDA) and glutathione (GSH), the activity of superoxide dismutase (SOD), and protein content were measured. Western blotting for the expression of Bax, Bcl-2, TNF-α, and NF-κB(p65) was also carried out. The effect of TFA was compared with that of standard antiulcer drug omeprazole (100 mg/kg). Results. This gastroprotective effect of TFA could be attributed to the increase in the activity of SOD and GSH and decrease in the levels of MDA and also decrease in the levels of Bax, TNF-α, and NF-κB(p65) expressions and increase in the Bcl-2 expression level. Conclusion. The findings of this study demonstrated that TFA could significantly attenuate ethanol-induced gastric injury via antioxidative, anti-inflammatory, and antiapoptotic effects.

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