Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers

Background. Abelmoschus manihot (L.) Medic flower is a medicinal plant for the treatment of diseases in China. The present study was carried out to scientifically validate the gastroprotective activity and clarify the possible mechanism of the total flavones from Abelmoschus manihot (L.) Medic flowers (TFA). Methods. Gastric ulcer was induced in mice by oral administration of ethanol. The gastroprotective activity of TFA was evaluated by the gastric ulcer index and histological examinations. The gastric tissue was collected in the form of homogenate. The level of malondialdehyde (MDA) and glutathione (GSH), the activity of superoxide dismutase (SOD), and protein content were measured. Western blotting for the expression of Bax, Bcl-2, TNF-α, and NF-κB(p65) was also carried out. The effect of TFA was compared with that of standard antiulcer drug omeprazole (100 mg/kg). Results. This gastroprotective effect of TFA could be attributed to the increase in the activity of SOD and GSH and decrease in the levels of MDA and also decrease in the levels of Bax, TNF-α, and NF-κB(p65) expressions and increase in the Bcl-2 expression level. Conclusion. The findings of this study demonstrated that TFA could significantly attenuate ethanol-induced gastric injury via antioxidative, anti-inflammatory, and antiapoptotic effects.

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Evaluation of Gastroprotective Activity of Cemot Leaves (Passiflora foetida L.) Extracted by Ultrasonic Assisted Extraction (UAE) Against Ethanol-Induced Gastric Lesions in Rats

Majalah Obat Tradisional ◽

10.22146/mot.53691 ◽

2020 ◽

Vol 25 (2) ◽

pp. 108

Author(s):

Elsa Trinovita ◽

Fatmaria Fatmaria

Keyword(s):

Gastric Ulcer ◽

Ethanol Extract ◽

Peptic Ulcers ◽

Gastroprotective Activity ◽

Ulcer Index ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction ◽

Passiflora Foetida ◽

Method Of Extraction

Peptic ulcers are one of the global health problems in the world. However, most of these drugs have unwanted side effects and drug interactions that this research focuses on the use of plant products as an anti gastric ulcer. The phytochemical of Passiflora foetida L. leaves or better known by the name of the Central Borneo with 'Cemot' such as flavonoids, alkaloids, and tannin has potential as a gastroprotective. This study used the method of extraction of non-conventional, namely the UAE, because it has advantages compared with other conventional methods. This study aims to investigate the gastroprotective activities of ethanol extract of Passiflora foetida leaves-UAE. Cemot extract was obtained by UAE method using a concentration of ethanol (70%), the ratio of sample/liquid (10g/mL) for 3 minutes. Three groups of rats treated with a respective dose of the extract (50, 100 and 200 mg/kg BW), one group of rats treated with omeprazole 36 mg/kg BW for seven days before peptic ulcer induction using ethanol. The activity gastroprotective with measurement of lesion of gastric ulcer index and the percentage of inhibition ulcer. Group of rats treated with 200 mg/kg BW extract showed a decreased the ulcer index and ulcer inhibition than the other extract dosage groups, respectively 2.83 and 35.34%. Conclusion: Based on the results of this study concluded that the cemot extract has potential as a gastroprotective.

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Gastro-protective actions of Aloe barbadensis Miller mitigate ethanol-induced gastric injury in rats

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i12.24 ◽

2021 ◽

Vol 19 (12) ◽

pp. 2645-2650

Author(s):

Kai Zhu ◽

Xia Yang ◽

Chun Yang ◽

Xiaoxue Ye ◽

Hongxing Zhang

Keyword(s):

Lipid Peroxidation ◽

Gastric Ulcer ◽

Gastric Mucosa ◽

Gastric Juice ◽

Histological Examination ◽

Leaf Extract ◽

Gastric Injury ◽

Control Group ◽

Gastric Tissue ◽

Aloe Barbadensis

Purpose: To investigate the gastroprotective effect of leaf extract of Aloe barbadensis on ethanolinduced gastric ulcer in rats.Methods: Healthy male Wistar rats (n = 30) weighing 180 - 220 g (mean weight = 200 ± 20 g) were randomly assigned to 6 groups (5 rats/group): control group, gastric ulcer group, two Aloe barbadensis extract groups (250 and 500 mg/kg), cimetidine group and indomethacin group. Gastric ulcer was induced via oral injection of absolute ethanol at a dose of 1 mL/kg after a 24-h fast. Gross evaluation, determination of gastric juice acidity and histological examination of gastric tissue were carried out.Results: Treatment of gastric ulcer rats with Aloe barbadensis extract significantly reduced ulcerated area (UA), ulcer index (UI), and acidity of gastric juice (p < 0.05). Injection of 1 % carrageenan into rat hind paw led to a time-dependent increase in paw volume which peaked 3 h after injection. However, the Aloe barbadensis extract significantly and dose-dependently reduced the volume of inflamed paw, and inhibited edema formation (p < 0.05). It also markedly reduced or completely eliminated edema and leucocyte infiltration. Moreover, treatment of gastric ulcer rats with Aloe barbadensis leaf extract led to significant and dose-dependent reduction in gastric tissue MDA level (p < 0.05). Histological examination of the gastric wall showed that control rats had severe injury in gastric mucosa,accompanied by edema and leucocytes infiltration, relative to rats pretreated with extract which showed marked gastric protection and inhibition of edema and leucocytes infiltration. Moreover, the extract treatment protected the gastric surface against ulceration as indicated by reduced lesions in the treated rat model.Conclusion: These results show that Aloe barbadensis mitigates ethanol-induced gastric injury in rats via inhibition of lipid peroxidation and inflammation. Thus, the extract has potentials for development into a therapeutic agent for the management of gastric ulcer. Keywords: Aloe barbadensis, Gastric ulcer, Gastric mucosa, Inflammation, Lipid peroxidation

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The Therapeutic Effect of Nigella sativa Seed on Indomethacin-induced Gastric Ulcer in Rats

Current Nutrition & Food Science ◽

10.2174/1573401315666190114152855 ◽

2020 ◽

Vol 16 (3) ◽

pp. 276-283

Author(s):

Maryam Paseban ◽

Saeed Niazmand ◽

Mohammad Soukhtanloo ◽

Naser T. Meibodi ◽

Abbasali Abbasnezhad ◽

...

Keyword(s):

Gastric Ulcer ◽

Acid Secretion ◽

Protein Content ◽

Gastric Acid Secretion ◽

Gastric Acid ◽

Therapeutic Effect ◽

Nigella Sativa ◽

Gastric Mucus ◽

Ulcer Index ◽

Nigella Sativa Seed

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to relieve pain and reduce inflammation. However, gastric complications remain a major problem limiting their clinical usage. This study was carried out to evaluate the therapeutic effect of Nigella sativa seed (N. sativa seed) hydroalcoholic extract on indomethacin-induced gastric ulcer in rats and its possible mechanism. Methods: This study was carried out on forty-eight male Wistar rats. Gastric ulcer was induced by indomethacin (35 mg/kg). N. sativa seed extract (100, 200, and 400 mg/kg) and ranitidine (50 mg/kg) was administered orally for five days after ulcer induction. Ulcer index, gastric acid secretion, gastric mucus content, total thiol, malondialdehyde (MDA), and total hexose, and protein content in gastric juice were determined. Results: The ulcer index in groups of N. sativa seed was significantly lower as compared to indomethacin group. N. sativa seed significantly decreased MDA and protein content, but increased total thiol, total hexose, and mucus content as compared to indomethacin group. N. sativa seed did not affect gastric acid secretion. Conclusion: These findings showed that the gastroprotective effect of N. sativa seed against indomethacin- induced ulcer was mainly exerted by antioxidant activity, stimulation of gastric mucus secretion and also increased total hexose in the gastric mucosa.

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The preventive effect of Nigella sativa seed on gastric ulcer induced by indomethacin in rat

Journal of Herbmed Pharmacology ◽

10.15171/jhp.2020.02 ◽

2020 ◽

Vol 9 (1) ◽

pp. 12-19

Author(s):

Maryam Paseban ◽

Saeed Niazmand ◽

Mohammad Soukhtanloo ◽

Naser Tayyebi Meybodi

Keyword(s):

Gastric Ulcer ◽

Acid Secretion ◽

Protein Content ◽

Gastric Acid Secretion ◽

Gastric Acid ◽

Nigella Sativa ◽

Gastric Ulceration ◽

Gastric Mucus ◽

Ulcer Index ◽

Nigella Sativa Seed

Introduction: Nonsteroidal anti-inflammatory drugs (NSAIDs) are considered as one of the most administrated groups of medications worldwide. Due to the role of NSAIDs in inducing gastric ulceration, their clinical applications are still challenging. Nigella sativa seed is widely used as an herbal medication against gastrointestinal complications. The present experiment was carried out to investigate the impact of N. sativa seed hydro-alcoholic extract on gastric ulcer induced by indomethacin (IND) and to evaluate its possible mechanisms in rat. Methods: This study was performed on 48 male Wistar rats. Acute gastric ulceration was induced by IND (35 mg/kg). N. sativa seed extract (100, 200, 400 mg/kg) and ranitidine (50 mg/kg) were administered orally for five days before the induction ulcer. Ulcer index, gastric acid secretion, gastric mucus content, glutathione (GSH), malondialdehyde (MDA), total hexose, gastric juice protein content were determined on the fifth day. Results: The ulcer index in all groups of N. sativa seed was significantly lower than that of the IND group. N sativa seed considerably decreased MDA and protein content, but increased total thiol, total hexose, and mucus content compared to the IND group. N. sativa seed did not affect gastric acid secretion. Conclusion: These findings were indicative of the gastroprotective effect of N. sativa seed against the IND-induced ulcer, suggesting that it can mainly be exerted through the anti-oxidant activity of the extract as well as its role in stimulating gastric mucus secretion and increasing total hexose in the gastric mucosa.

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Optimized Ellagic Acid–Ca Pectinate Floating Beads for Gastroprotection against Indomethacin-Induced Gastric Injury in Rats

Biomolecules ◽

10.3390/biom10071006 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1006 ◽

Cited By ~ 1

Author(s):

Nabil A. Alhakamy ◽

Shaimaa M. Badr-Eldin ◽

Osama A. A. Ahmed ◽

Abdulrahman A. Halwani ◽

Hibah M. Aldawsari ◽

...

Keyword(s):

Gastric Ulcer ◽

Ellagic Acid ◽

Gastric Ulcers ◽

Gastric Injury ◽

Parameter Determination ◽

Ulcer Index ◽

Oxidative Stress Parameter ◽

Mucosal Defect ◽

Inflammatory Drugs

A peptic ulcer is an alimentary tract injury that leads to a mucosal defect reaching the submucosa. This work aimed to optimize and maximize ellagic acid (EA) loading in Ca pectinate floating beads to maximize the release for 24 h. Three factors were selected: Ca pectinate concentration (X1, 1–3 w/v %), EA concentration (X2, 1–3 w/v %) and the dropping time (X3, 10–30 min). The factorial design proposed eight formulations. The optimized EA–Ca pectinate formulation was evaluated for the gastric ulcer index and the oxidative stress parameter determination of gastric mucosa. The results indicated that the optimum EA–Ca pectinate formula significantly improved the gastric ulcer index in comparison with raw EA. The protective effect of the optimized EA–Ca pectinate formula was further indicated by the histopathological features of the stomach. The results of the study indicate that an EA formulation in the form of Ca pectinate beads would be effective for protection against gastric ulcers because of Nonsteroidal anti-inflammatory drugs (NSAID) administration.

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Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats

Pharmaceutics ◽

10.3390/pharmaceutics12020140 ◽

2020 ◽

Vol 12 (2) ◽

pp. 140 ◽

Cited By ~ 3

Author(s):

Osama A. A. Ahmed ◽

Usama A. Fahmy ◽

Rana Bakhaidar ◽

Mohamed A. El-Moselhy ◽

Solomon Z. Okbazghi ◽

...

Keyword(s):

Gastric Ulcer ◽

Gastric Injury ◽

Tween 20 ◽

Protective Effects ◽

Omega 3 ◽

Ulcer Index ◽

Ulcer Disease ◽

Mucosal Defect ◽

Protection Efficacy ◽

Mixture Experimental Design

Peptic ulcer disease is an injury of the alimentary tract that leads to a mucosal defect reaching the submucosa. This study aimed to formulate and optimize omega-3 oil as a self-nanoemulsifying drug delivery system (SNEDDS) to achieve oil dispersion in the nano-range in the stomach to augment omega-3 oil gastric ulcer protection efficacy. Three SNEDDS components were selected as the design factors: the concentrations of the oil omega-3 (X1, 10–30%), the surfactant tween 20 and Kolliphor mixture (X2, 20–40%), and the cosurfactant transcutol (X3, 40–60%). The mixture experimental design proposed twenty-three formulations with varying omega-3 SNEDDS formulation component percentages. The optimized omega-3 SNEDDS formula was investigated for gastric ulcer protective effects by evaluating the ulcer index and by the determination of gastric mucosa oxidative stress parameters. Results revealed that optimized omega-3-SNEDDS achieved significant improvement in the gastric ulcer index in comparison with pure omega-3 oil. Histopathological findings confirmed the protective effect of the formulated optimized omega-3 SNEDDS in comparison with omega-3 oil. These findings suggest that formulation of omega-3 in the form of a SNEDDS would be more effective in gastric ulcer protection than the administration of omega-3 as a crude oil.

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Preventive Effect of Liupao Tea Polyphenols on HCl/Ethanol-Induced Gastric Injury in Mice

Journal of Food Quality ◽

10.1155/2020/5462836 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Kai Zhu ◽

Peng Peng ◽

Ning Wu ◽

Xianrong Zhou ◽

Jianfei Mu ◽

...

Keyword(s):

Gastric Juice ◽

Tea Polyphenols ◽

Gastric Injury ◽

Mouse Serum ◽

Gastric Tissue ◽

Preventive Effect ◽

High Concentration ◽

Sod Activity ◽

Bioactive Substances ◽

Gastric Mucosal Lesions

Liupao tea is a traditional Chinese tea drink. The preventive effect of crude polyphenols in Liupao tea on HCl/ethanol-induced gastric injury was investigated in this study. After a model of gastric injury in mice was established, mouse serum and tissues were analyzed by biochemical and molecular biological methods. The results showed that Liupao tea polyphenols (LTPs) could effectively reduce the area of gastric mucosal lesions, decrease the volume of gastric juice, and increase the pH of gastric juice in mice with gastric injury. Observations of the pathology revealed that LTPs could alleviate cell necrosis and gastric mucosal injury in mice with gastric injury. The SOD activity and GSH level were decreased in mice after gastric injury, while the level of MDA was increased. LTPs could inhibit the changes caused by gastric injury and make the SOD activity, GSH, and MDA levels close to the normal levels. In addition, LTPs could upregulate the mRNA expression of Cu/Zn-SOD, Mn-SOD, CAT, nNOS, and eNOS and downregulate the expression of iNOS in the gastric tissue of mice with gastric injury. Therefore, LTPs can effectively prevent HCl/ethanol-induced gastric injury. HPLC analysis showed that LTP contains six bioactive substances of gallic acid, catechin, caffeine, epicatechin, epigallocatechin gallate, and epicatechin gallate, so the effect of LTP might mainly come from these six components. The effect of a high concentration of LTP is similar to that of ranitidine. LTPs represent a kind of active substance with a protective effect on gastric tissue.

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Optimized Self-Nanoemulsifying Delivery System Based on Plant-Derived Oil Augments Alpha-Lipoic Acid Protective Effects Against Experimentally Induced Gastric Lesions

Dose-Response ◽

10.1177/15593258211001259 ◽

2021 ◽

Vol 19 (1) ◽

pp. 155932582110012

Author(s):

Shaimaa M. Badr-Eldin ◽

Usama A. Fahmy ◽

Hibah M. Aldawsari ◽

Osama A. A. Ahmed ◽

Nabil A. Alhakamy ◽

...

Keyword(s):

Gastric Ulcer ◽

Delivery System ◽

Lipoic Acid ◽

Tween 80 ◽

Protective Effects ◽

Alpha Lipoic Acid ◽

Ulcer Index ◽

Ulcer Disease ◽

Mucosal Defect ◽

Polyethylene Glycol 200

Peptic ulcer disease is an injury of the alimentary tract that leads to a mucosal defect reaching the submucosa. Alpha-lipoic acid (ALA), a natural potent antioxidant, has been known as a gastroprotective drug yet its low bioavailability may restrict its therapeutic efficacy. This study aimed to formulate and optimize ALA using a self-nanoemulsifying drug delivery system (SNEDDS) with a size of nano-range, enhancing its absorption and augmenting its gastric ulcer protection efficacy. Three SNEDDS components were selected as the design factors: the concentrations of the pumpkin oil (X1, 10–30%), the surfactant tween 80 (X2, 20–50%), and the co-surfactant polyethylene glycol 200 (X3, 30–60%). The experimental design for the proposed mixture produced 16 formulations with varying ALA-SNEDDS formulation component percentages. The optimized ALA-SNEDDS formula was investigated for gastric ulcer protective effects by evaluating the ulcer index and by the determination of gastric mucosa oxidative stress parameters. Results revealed that optimized ALA-SNEDDS achieved significant improvement in gastric ulcer index in comparison with raw ALA. Histopathological findings confirmed the protective effect of the formulated optimized ALASNEDDS in comparison with raw ALA. These findings suggest that formulation of ALA in SNEDDS form would be more effective in gastric ulcer protection compared to pure ALA.

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Evaluation of a poly-herbal preparation for the treatment of peptic ulcer

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v12i2.29980 ◽

2017 ◽

Vol 12 (2) ◽

pp. 11

Author(s):

Zaman Hayat ◽

Mueen Ahmad Chaudhry ◽

. Alamgeer

Keyword(s):

Peptic Ulcer ◽

Gastric Ulcer ◽

Treated Group ◽

Ulcer Formation ◽

Ulcer Index ◽

Herbal Formulation ◽

High K ◽

Ulcer Model ◽

Dose Dependent ◽

Gross Examination

<p class="Abstract">The aim of this study was to validate the traditional uses of ulcerene, a poly-herbal formulation in ethanol, aspirin and stress-induced gastric ulcer model of rat. The extent of gastric ulcer formation was studied, using ulcer score, ulcer index, percentage cure through gross examination and histopathological evaluation. A significant (p<0.001) dose-dependent anti-ulcerant effect was observed in ulcerene (50 and 100 mg/kg)-treated group with highest effectiveness against ethanol-induced ulcer. The concentration-dependent spasmolytic effect was seen in spontaneously contracting, high K<sup>+</sup> (80 mM) and carbachol (1 µM)-induced jejunum contractions (10, 0.3 and 1 mg/mL), similar to dicyclomine (10, 1 and 3 µM), indicated non-specific spasmolytic mechanism behind the effect. By considering these results, ulcerene can be suggested for the treatment of peptic ulcer.</p>

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