Stabilization of Refined Olive Oil with Phenolic Monomers Fraction and Purified Hydroxytyrosol from Olive Mill Wastewater

2020 ◽  
Vol 3 (3) ◽  
pp. 657-665
Author(s):  
Amina Ben Saad ◽  
Amel Jerbi ◽  
Ines Khlif ◽  
Mohamed Ayedi ◽  
Noureddine Allouche
Keyword(s):  
Olive Oil ◽  
Olive Mill Wastewater ◽  
Refined Olive Oil ◽  
Olive Mill

scholarly journals Antioxidant Properties of Olive Mill Wastewater Polyphenolic Extracts on Human Endothelial and Vascular Smooth Muscle Cells

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 800
Author(s):  
Anna Maria Posadino ◽  
Annalisa Cossu ◽  
Roberta Giordo ◽  
Amalia Piscopo ◽  
Wael M Abdel-Rahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Death ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Olive Oil ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Olive Mill Wastewater ◽  
Olive Mill

This work aims to analyze the chemical and biological evaluation of two extracts obtained by olive mill wastewater (OMW), an olive oil processing byproduct. The exploitation of OMW is becoming an important aspect of development of the sustainable olive oil industry. Here we chemically and biologically evaluated one liquid (L) and one solid (S) extract obtained by liquid–liquid extraction followed by acidic hydrolysis (LLAC). Chemical characterization of the two extracts indicated that S has higher phenol content than L. Hydroxytyrosol and tyrosol were the more abundant phenols in both OMW extracts, with hydroxytyrosol significantly higher in S as compared to L. Both extracts failed to induce cell death when challenged with endothelial cells and vascular smooth muscle cells in cell viability experiments. On the contrary, the higher extract dosages employed significantly affected cell metabolic activity, as indicated by the MTT tests. Their ability to counteract H2O2-induced oxidative stress and cell death was assessed to investigate potential antioxidant activities of the extracts. Fluorescence measurements obtained with the reactive oxygen species (ROS) probe H2DCF-DA indicated strong antioxidant activity of the two OMW extracts in both cell models, as indicated by the inhibition of H2O2-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate LLAC-obtained OMW extracts as a safe and useful source of valuable compounds harboring antioxidant activity.

scholarly journals Intergraded Applied Methodology for the Treatment of Heavy Polluted Waste Waters from Olive Oil Industries

2011 ◽  
Vol 2011 ◽  
pp. 1-14 ◽  
Author(s):  
Antonis A. Zorpas ◽  
Vassilis J. Inglezakis
Keyword(s):  
Olive Oil ◽  
Oil Production ◽  
Olive Mill Wastewater ◽  
Fenton Process ◽  
Waste Waters ◽  
Warm Climate ◽  
Climate Conditions ◽  
Integrated Method ◽  
Third Stage ◽  
Olive Mill

The annual olive oil production in Cyprus is in the range of 2700–3100 t y−1, resulting in the generation of significant amount of waste. The cocomposting of the olive oil solid residue (OOSR) and the treated wastewaters (with Fenton) from the olive oil production process with the application of reed beds has been studied as an integrated method for the treatment of wastewater containing high organic and toxic pollutants under warm climate conditions. The experimental results indicated that the olive mill wastewater (OMW) is detoxified at the end of the Fenton process. Specifically, COD is reduced up to 65% (minimum 54.32%) by the application of Fenton and another 10–28% by the application of red beds as a third stage. The final cocomposted material of OOSR with the treated olive mile wastewater (TOMW) presents optimum characteristics and is suitable for agricultural purpose.

A focus on pressure-driven membrane technology in olive mill wastewater reclamation: state of the art

2012 ◽  
Vol 66 (12) ◽  
pp. 2505-2516 ◽  
Author(s):  
J. M. Ochando-Pulido ◽  
A. Martinez-Ferez
Keyword(s):  
Olive Oil ◽  
Large Scale ◽  
Wastewater Treatment Plants ◽  
Domestic Wastewater ◽  
Olive Mill Wastewater ◽  
Operating Conditions ◽  
Added Value ◽  
Wastewater Reclamation ◽  
Starting Point ◽  
Olive Mill

Direct disposal of the heavily polluted effluent from olive oil industry (olive mill wastewater, OMW) to the environment or to domestic wastewater treatment plants is actually prohibited in most countries, and conventional treatments are ineffective. Membranes are currently one of the most versatile technologies for environmental quality control. Notwithstanding, studies on OMW reclamation by membranes are still scarce, and fouling inhibition and prediction to improve large-scale membrane performance still remain unresolved. Consequently, adequately targeted pretreatment for the specific binomium membrane-feed, as well as optimized operating conditions for the proper membranes, is today's challenge to ensure threshold flux values. Several membrane materials, configurations and pore sizes have been elucidated, and also different pretreatments including sedimentation, centrifugation, biosorption, sieving, filtration and microfiltration, various types of flocculation as well as advance oxidation processes have been applied so far. Recovery of potential-value compounds, such as a variety of polyphenols highlighting oleuropein and hydroxytyrosol, has been attempted too. All this research should constitute the starting point to proceed with OMW purification beyond recycling for irrigation or depuration for sewer discharge, with the aim of complying with standards to reuse the effluent in the olive oil production process, together with cost-effective recovery of added-value compounds.

Long-term field evaluation of the changes in fruit and olive oil chemical compositions after agronomic application of olive mill wastewater with rock phosphate

2018 ◽  
Vol 239 ◽  
pp. 664-670 ◽  
Author(s):  
Meriem Tekaya ◽  
Sinda El-Gharbi ◽  
Hechmi Chehab ◽  
Faouzi Attia ◽  
Mohamed Hammami ◽  
...  
Keyword(s):  
Olive Oil ◽  
Rock Phosphate ◽  
Field Evaluation ◽  
Olive Mill Wastewater ◽  
Chemical Compositions ◽  
Olive Mill ◽  
Term Field

scholarly journals Enrichment of Phenolic Compounds from Olive Mill Wastewater and In Vitro Evaluation of Their Antimicrobial Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Saleh Abu-Lafi ◽  
Mahmoud Sami Al-Natsheh ◽  
Reem Yaghmoor ◽  
Fuad Al-Rimawi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Acid Value ◽  
Antimicrobial Activities ◽  
Olive Mill Wastewater ◽  
Total Phenolic ◽  
The Stability ◽  
Olive Mill

The production of olive oil generates massive quantities of by-product called olive mill wastewater (OMWW). The uncontrolled disposal of OMWW poses serious environmental problems. The OMWW effluent is rich in several polyphenolic compounds. Liquid-liquid extraction of OMWW using ethyl acetate solvent was used to enrich phenolic compounds under investigation. Total phenolic and flavonoid content and antioxidant activity of the extract were determined. HPLC coupled to photodiode array (PDA) detector was used to analyze the main three phenolic compounds of OMWW, namely, hydroxytyrosol, tyrosol, and oleuropein. The antimicrobial activity of the extract was also investigated. Additionally, the OMWW extract was used as natural preservative and antioxidants for olive oil. Results showed that OMWW is very rich in phenolic compounds and has strong antioxidant activity. HPLC analysis showed that the extract contains mainly hydroxytyrosol and tyrosol but no oleuropein. The OMWW extract showed also positive activities as antibacterial (gram positive and gram negative) and antifungal as well as activities against yeast. The addition of OMWW extract to olive oil samples has an effect on the stability of olive oil as reflected by its acid value, peroxide value, K232 and K270, and total phenolic content.

Treatment of olive-mill wastewater from a two-phase process by chemical oxidation on an industrial scale

2009 ◽  
Vol 59 (10) ◽  
pp. 2017-2027 ◽  
Author(s):  
L. M. Nieto ◽  
G. Hodaifa ◽  
S. R. Vives ◽  
J. A. G. Casares ◽  
S. B. Driss ◽  
...  
Keyword(s):  
Olive Oil ◽  
Oil Industry ◽  
Total Suspended Solid ◽  
Chemical Oxidation ◽  
Suspended Solid ◽  
Olive Mill Wastewater ◽  
Two Phase ◽  
Oil Companies ◽  
Olive Oil Mills ◽  
Olive Mill

This study offers a solution for reducing the environmental effect of wastewaters generated by the olive-oil industry. Olive-oil companies produce variable quantities of wastewaters, which require treatment for disposal or reuse. Today, regulations are becoming increasingly strict regarding the parameters measured in these effluents. In Spain, the resolution by the president of the Hydrographical Confederation of the Guadalquivir on water use 2004 set parameter limits as follows: pH = 6.0–9.0, total suspended solid = 500 mg/L; and COD and BOD520=1,500 mg O2/L. For the year 2006, maximum values for COD and BOD520 were fixed at 1,000 mg O2/L. To solve this problem, a study has been made to derive irrigation water from the above-mentioned effluents through chemical oxidation based on the Fenton's process. This would be first step towards using a closed-circuit system in olive-oil mills to treat and reuse effluents.

scholarly journals Time domain reflectometry for dielectric characterization of olive mill wastewater contaminated soils

2020 ◽  
Vol 51 (4) ◽  
pp. 248-254
Author(s):  
Alessandro Comegna ◽  
Antonio Coppola ◽  
Giovanna Dragonetti
Keyword(s):  
Olive Oil ◽  
Time Domain ◽  
Contaminated Soils ◽  
Oil Industry ◽  
Time Domain Reflectometry ◽  
Olive Mill Wastewater ◽  
Management Problem ◽  
Dielectric Characterization ◽  
Olive Mill

Olive mill wastewater (OMW) is a compound originating from oil mills during oil extraction processes. In the Mediterranean area, more than 30 million m3 of OMW are produced each year, which represents 95-97% of the world production. Such volumes of untreated OMW are usually directly disposed of into drainage systems, water bodies (such as streams, lagoons and ponds) or are sprinkled on soils, causing potentially severe environmental problems to soils and groundwater. Consequently, there is a serious waste management problem related to the olive oil industry, because these practices no longer being acceptable. In the case of on-land OMW disposal, the characterization and the identification of this contaminant in soils is a fundamental task especially with a view to maintaining the integrity and quality of agroecosystems. In recent years, soils have been extensively studied to detect contaminants by using various geophysical methods. Among such techniques, time domain reflectometry (TDR) has shown, in different contexts, evident sensitivity and resolution capability for the characterization of contaminated soil sites. In order to further exploit the potential of the TDR technique, in this study we conducted a series of laboratory-controlled tests to explore how OMW influences the dielectric response of contaminated soils. This investigation led to the development of an empirical dielectric model to estimate the presence of OMW in variably saturated-contaminated soils with different textures and pedological features. In particular, the soils selected belong to two typical pedological units in southern Italy which account for approximately 90% of the Italian olive plantations and one of the highest concentrations of the olive oil industry in Italy. In these districts, as well as in other European and Mediterranean countries, there is the controversial habit to spread OMW on soils.

scholarly journals Monitoring Olive Oil Mill Wastewater Disposal Sites Using Sentinel-2 and PlanetScopeSatellite Images: Case Studies in Tunisia and Greece

Agronomy ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 90
Author(s):  
Wissal Issaoui ◽  
Dimitrios D. Alexakis ◽  
Imen Hamdi Nasr ◽  
Athanasios V. Argyriou ◽  
Evangelos Alevizos ◽  
...  
Keyword(s):  
Olive Oil ◽  
Satellite Images ◽  
Vegetation Index ◽  
Satellite Image ◽  
Olive Mill Wastewater ◽  
Support Vector ◽  
Inorganic Particles ◽  
Mediterranean Countries ◽  
Sentinel 2 ◽  
Olive Mill

Mediterranean countries are known worldwide for their significant contribution to olive oil production, which generates large amounts of olive mill wastewater (OMW) that degrades land and water environments near the disposal sites. OMW consists of organic substances with high concentrations of phenolic compounds along with inorganic particles. The aim of this study is to assess the effectiveness of satellite image analysis techniques using multispectral satellite data with high (PlanetScope, 3 × 3 m) and medium (Sentinel-2, 10 × 10 m) spatial resolution to detect Olive Mill Wastewater (OMW) disposal sites, both in the SidiBouzid region (Tunisia) and in the broader Rethymno region on the island of Crete, (Greece). Documentation of the sites was carried out by collecting spectral signatures of OMW at temporal periods. The study integrates the application of a variety of spectral vegetation indices (VIs), such as the Normalized Difference Vegetation Index (NDVI), in order to evaluate their efficiency in detecting OMW disposal areas. Furthermore, a set of image-processing methods was applied on satellite images to improve the monitoring of OMW ponds including the false-color composites (FCC), the Principal Component Analysis (PCA), and image fusion. Finally, different classification algorithms, such as the ISODATA, the maximum likelihood (ML), and the Support Vector Machine (SVM) were applied to both satellite images in order to assist in the overall approach to effectively detect the sites. The results obtained from different approaches were compared, evaluating the efficiency of Sentinel-2 and PlanetScope images to detect and monitor OMW disposal areas under different morphological environments.

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