Isoenzyme profiles of creatine kinase, lactate dehydrogenase and aspartate aminotransferase in the reperfused myocardium K. Kawaguchi, Y. Awaji, K. Ishikawa, H. Hashimoto, K. Okumura, T. Ito, T. Satake. Second Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan

1991 ◽  
Vol 23 ◽  
pp. 20
Keyword(s):  
Internal Medicine ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
School Of Medicine ◽  
Reperfused Myocardium

Production and certification of secondary enzyme reference materials (ERMs). Part 1: Preparation of the sera and some of their properties.

1986 ◽  
Vol 32 (10) ◽  
pp. 1901-1905 ◽  
Author(s):  
J C Koedam ◽  
G M Steentjes ◽  
S Buitenhuis ◽  
E Schmidt ◽  
R Klauke
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Reference Material ◽  
Human Serum ◽  
Dehydrogenase Activity ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Glutamate Dehydrogenase Activity ◽  
The Stability ◽  
Clinical Enzymology

Abstract We produced three batches of a human-serum-based enzyme reference material (ERM) enriched with human aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27). The added enzymes were not exhaustively purified; thus the final ERMs contained some enzymes as contaminants, of which only glutamate dehydrogenase activity might interfere. The stability during storage and after reconstitution was good. The commutability of the four enzymes in the three ERM batches was also good, except when German or Scandinavian methods for aminotransferases were involved. The temperature-conversion factors for the ERMs were equivalent to those for patients' sera. Reactivation after reconstitution was complete within 5 min and was independent of the temperature of the reconstitution fluid. We believe that these secondary ERMs will aid in the transfer of accuracy between well-defined reference methods and daily working methods so that clinical enzymology results will become more comparable from laboratory to laboratory.

scholarly journals Local myotoxicity of ketamine hydrochloride in the marmoset

1987 ◽  
Vol 21 (1) ◽  
pp. 60-67 ◽  
Author(s):  
C. W. Davy ◽  
P. N. Trennery ◽  
J. G. Edmunds ◽  
J. F. B. Altman ◽  
D. A. Eichler
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Physical Properties ◽  
Aspartate Aminotransferase ◽  
Solution Ph ◽  
Histopathological Findings ◽  
Plasma Enzyme ◽  
Routine Blood ◽  
Ketamine Hydrochloride ◽  
Injection Procedure

An investigation of raised plasma aspartate aminotransferase (AST) in marmosets after intramuscular ketamine injection suggested a local myotoxicity. This was confirmed by a range of histopathological findings from myofibrillar striation loss to necrosis. In addition to the elevations in AST levels, creatine kinase and the lactate dehydrogenase-5 isoenzyme levels were elevated. It was further demonstrated that, although the physical properties of the injectable solution (pH, osmolality) and to a lesser extent the injection procedure itself caused slight changes in plasma enzyme levels, the ketamine was predominantly responsible for the lesion. No hepatic interactions were seen. This effect should be taken into consideration when this anaesthetic is used in the marmoset if the primary objectives of the experiment entail routine blood analyses.

scholarly journals Effect of Various Diluents on the Activity of Several Enzymes Present in Serum

1973 ◽  
Vol 19 (9) ◽  
pp. 1079-1080
Author(s):  
Ted W Fendley ◽  
Jane M Hochholzer ◽  
Christopher S Frings
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Confidence Level ◽  
Aspartate Aminotransferase ◽  
Nacl Solution ◽  
Manual Method ◽  
Accuracy And Precision ◽  
Significant Difference

Abstract We have evaluated the effect of diluting serum with water or NaCl solution (8.5 or 9.0 g/liter) before assaying by a manual method for creatine kinase (EC 2.7.3.2), alkaline phosphatase (EC 3.1.3.1), lactate dehydrogenase (EC 1.1.1.27), and aspartate aminotransferase (EC 2.6.1.1) activity. The t test and the F test show no significant difference in the accuracy and precision of the assays at the 95% confidence level when 100 different samples were compared for each enzyme activity after use of the three diluents.

scholarly journals Cardiac Safety of Diclofenac at a Single Dose in Ram

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Ayse Er ◽  
Burak Dik ◽  
Orhan Corum ◽  
Gul Cetin
Keyword(s):  
Creatine Kinase ◽  
Single Dose ◽  
Lactate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Troponin I ◽  
Density Lipoprotein ◽  
Cardiac Damage ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Lipoprotein Level

Nonsteroidal anti-inflammatory drugs are frequently prescribed drug group in human and veterinary medicine. However, diclofenac, a traditional nonsteroidal anti-inflammatory drug, related to cardiotoxicity is reported, and blood cardiac damage markers may increase within the first hours after damage. The aim of the current research was to determine the effect of diclofenac on the blood cardiac damage markers. Single dose of diclofenac (2.5 mg/kg, IM) was injected to 6 rams. Blood samples were collected in before (0 hour, control) and 6 hours after injection. Specific (troponin I, and creatine kinase-MB) and nonspecific (lactate dehydrogenase, aspartate aminotransferase) blood cardiac damage marker concentrations, routine biochemical (hepatic damage, renal damage, lipid metabolism, glucose, and phosphorus) parameters, and hemogram values were measured. Diclofenac increased (P<0.05) specific (troponin I) and nonspecific cardiac (lactate dehydrogenase, aspartate aminotransferase), hepatic (aspartate aminotransferase, alkaline phosphatase, and alanine aminotransferase), and muscular (creatine kinase) damage markers and high density lipoprotein level, while it decreased (P<0.05) low density lipoprotein level. Moreover, diclofenac decreased (P<0.05) white blood cell counts and increased (P<0.05) red blood cell counts. In conclusion, it may be stated that diclofenac shows slight cardiotoxicity, whereas it may show potent hepatic and muscular damage effects at an intramuscularly single dose in sheep. Thereby, repeated injections of diclofenac may be more harmful in sheep.

scholarly journals Muscular Damage and Kidney Function in Rugby Players after Daily Whole Body Cryostimulation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Giovanni Lombardi ◽  
Alessandra Colombini ◽  
Simone Porcelli ◽  
Clara Mauri ◽  
Viviana Zani ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Serum Creatinine ◽  
Kidney Function ◽  
Cystatin C ◽  
Aspartate Aminotransferase ◽  
Whole Body ◽  
Lactate Dehydrogenase Activity ◽  
Rugby Players ◽  
Muscular Damage

Muscular damage, consequent to strenuous activities, could exceed the recovery potential of muscles and determine renal failure. Whole body cryostimulation is a cold-based therapy used to improve recovery or overcome fatigue symptoms. This study aimed to evaluate the effects of repeated sessions of cryostimulation on muscle damage, renal function, and their relationship. Serum samples, from 27 elite rugby players, under training, before and after 2 sessions/day of cryotherapy over 7 days, were tested for markers of muscular (creatine kinase, lactate dehydrogenase, and aspartate aminotransferase) and renal (creatinine, cystatin C) functions. eGFR was calculated with two formulas based on either serum creatinine concentration (MDRD) or serum creatinine and cystatin C concentrations (creatinine/cystatin C CKD EPI). Pre- and posttreatment differences were determined by Wilcoxon’s test; correlations were evaluated with Spearman’s test. Cryostimulation helped muscular recovery (increased lactate dehydrogenase activity (P<0.001) and the stabile creatine kinase and aspartate aminotransferase activities). Creatinine was unaffected while cystatin C was increased (P=0.013). Creatinine-based eGFR was not affected by cryostimulation, while creatinine/cystatin C-based eGFR showed a slight decrease (P=0.015). eGFR and muscular biomarkers were not correlated, suggesting a real recovery effect of cryotherapy. Cystatin C seemed more sensible than creatinine in evaluating the kidney function.

scholarly journals Effect of Gas Flare on Some Clinical Enzyme Biomarkers of Eleme Residents in Rivers State, Nigeria

2020 ◽  
pp. 101-107
Author(s):  
Tamuno-boma Odinga ◽  
Felix U. Igwe ◽  
Christine U. Gabriel-Brisibe ◽  
P. C. Dimkpa
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Oil And Gas ◽  
Continuous Exposure ◽  
Gas Flaring ◽  
Test Kit ◽  
The Impact ◽  
Male Subjects ◽  
Female Subjects

Background: Gas flaring, an environmental harmful practice, is prevalent in Eleme and most communities in the Niger Delta region of Nigeria. Aim: This study explored the impact of gas flaring on some clinical enzyme biomarkers of Eleme residents who are constantly exposed to the flared gas. Study Design/Place of Study: Volunteer subjects were randomly selected from representative groups resident in Eleme, an oil and gas producing and refining area, for over 15 years, while similar volunteer subjects resident in Nkpolu-Oroworukwo, a non-gas flaring community, served as the control. Methodology: Blood samples were collected from the subjects and analyzed for selected clinical enzyme biomarkers including Creatine kinase (CK), Lactate dehydrogenase (LDH) and Aspartate aminotransferase (AST) using standard enzyme activity and Randox test kit methods. Results: Results obtained revealed that CK levels for female Eleme subjects increased by 61.20% when compare with the female control subjects. This increase was statistically significant (p<0.05). The CK levels for Eleme male subjects also increased by 37.36% compared to control. However, this increase was not statistically significant (p<0.05). LDH increased by 23.21% in the male subjects and 18.58% in the female subjects compared to control, while AST increased by 42.11% in the male subject and 11.32% in the female subjects compared to control. The results suggest that there could be impending damage to organs for which an increase in the biomarkers-Creatine kinase, Lactate Dehydrogenase and Aspartate Aminotransferase suggest an ongoing pathologic process. Conclusion: These results therefore suggest that the continuous exposure to gas flare is causing an increase in some critical tissue and organ function enzyme biomarkers in blood. This may eventually affect the health status of the residents, increase the tendency of developing ill health and generally reduce their quality of life.

scholarly journals Changes in Salivary Levels of Creatine Kinase, Lactate Dehydrogenase, and Aspartate Aminotransferase after Playing Rugby Sevens: The Influence of Gender

2020 ◽  
Vol 17 (21) ◽  
pp. 8165
Author(s):  
Álvaro González Fernández ◽  
Jose Enrique de la Rubia Ortí ◽  
Lorena Franco-Martinez ◽  
Jose Joaquín Ceron ◽  
Gonzalo Mariscal ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Muscle Damage ◽  
Aspartate Aminotransferase ◽  
Perceived Exertion ◽  
Total Sample ◽  
Physical Contact ◽  
Borg Scale ◽  
Men And Women ◽  
Before And After

Rugby sevens is characterised by continuous exertion and great physical contact per unit of time, leading to muscle damage. It is important to identify markers that can quantify muscle damage in order to improve recovery strategies. The objective of this study was to evaluate the release dynamics of muscle damage markers creatine kinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) in saliva samples when playing rugby sevens, analysing the influence of gender, during the rugby sevens university championship of Spain. The total sample included 27 athletes, divided into two teams of 14 men and 13 women between 18 and 31 years of age. CK, LDH, and AST were quantified from salivary samples collected from each athlete before and after three rugby sevens matches. The modified Borg scale of perceived exertion was also used after each match. When the results were analysed globally, there were no differences in CK and LDH before and after any match, but AST did show differences after two days of completing all matches. In terms of gender, the three enzymes showed different responses in men and women. Regarding the Borg scale, there were only significant differences between men and women after completing all mataches, with a greater perceived exertion in women. Based on our results, it can be stated that that serial matches of rugby sevens can cause changes of different magnitude in AST, CK and LDH activities in saliva, with AST showing the most significant variations and these changes are more pronounced in men than in women.

Evaluation of a Gilford "Automatic Enzyme Analyzer" Modified to Assay 60 Samples per Hour

1974 ◽  
Vol 20 (10) ◽  
pp. 1295-1304 ◽  
Author(s):  
Samuel A Morell ◽  
Daniel A Bach ◽  
Valborg E Ayers
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Enzyme Assay ◽  
Linear Range ◽  
End Point ◽  
Precision And Accuracy ◽  
Mode 3

Abstract A Gilford 3400 Automatic Enzyme Analyzer has been modified to assay 60 samples per hour in modes 1 and 2 (28 and 61 seconds of incubation) or 30 samples per hour in mode 3 (187 seconds of incubation). The calculation cycle was reduced threefold to print activity based on ΔA/20 s, rather than ΔA/60 s. Linear ranges at 30 and 37 °C for lactate dehydrogenase, aspartate aminotransferase, and alkaline phosphatase were determined with control sera at rates of 60 samples per hour and for creatine kinase activated by dithiothreitol at 30 samples per hour. AutoAnalyzer end-point assays of patients' sera were correlated with the Gilford U/liter values. Application of the Micromedic pipette to the Gilford AEA system provided a convenient mean to study the linear range, precision, and accuracy of an enzyme assay.

Cardiac enzyme changes in myxedema coma.

1987 ◽  
Vol 33 (4) ◽  
pp. 622-624 ◽  
Author(s):  
P E Hickman ◽  
W Silvester ◽  
A A Musk ◽  
G H McLellan ◽  
A Harris
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Clinical Settings ◽  
Cardiac Enzyme ◽  
Myxedema Coma ◽  
Plasma Enzyme ◽  
Enzyme Changes

Abstract A 74-year-old man with myxedema and hypothermia had increased activities in plasma of creatine kinase (CK; EC 2.7.3.2), aspartate aminotransferase (AST; EC 2.6.1.1), and lactate dehydrogenase (LD; EC 1.1.1.27) and increased proportions of CK-MB (up to 20% of total CK) and LD1 isoenzymes, but no clinical or investigational evidence of associated myocardial infarction. This case illustrates that plasma enzyme activity and isoenzyme profiles in such clinical settings should be interpreted with caution, because increases in CK-MB and LD1 may relate to myxedema coma or hypothermia (or both) rather than to myocardial infarction.

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