Chemical, modulus and cell attachment studies of reactive calcium phosphate filler-containing fast photo-curing, surface-degrading, polymeric bone adhesives

2010 ◽  
Vol 6 (7) ◽  
pp. 2695-2703 ◽  
Author(s):  
E.A. Abou Neel ◽  
G. Palmer ◽  
J.C. Knowles ◽  
V. Salih ◽  
A.M. Young
Keyword(s):  
Calcium Phosphate ◽  
Cell Attachment ◽  
Photo Curing ◽  
Phosphate Filler

scholarly journals Special Issue: Novel Advances and Approaches in Biomedical Materials Based on Calcium Phosphates

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 405 ◽  
Author(s):  
Michael Mucalo
Keyword(s):  
Calcium Phosphate ◽  
Cell Attachment ◽  
Calcium Phosphates ◽  
Wide Spectrum ◽  
Special Issue ◽  
Dolomitic Marble ◽  
Biomedical Materials ◽  
Surface Contaminants ◽  
Innovative Solutions ◽  
Enhance Cell Attachment

Research on calcium phosphate use in the development and clinical application of biomedical materials is a diverse activity and is genuinely interdisciplinary, with much work leading to innovative solutions for improvement of health outcomes. This Special Issue aimed to summarize current advances in this area. The nine papers published cover a wide spectrum of topical areas, such as (1) remineralisation pastes for decalcified teeth, (2) use of statins to enhance bone formation, (3) how dolomitic marble and seashells can be processed into bioceramic materials, (4) relationships between the roughness of calcium phosphate surfaces and surface charge with the effect on human MRC osteogenic differentiation and maturation being investigated, (5) rheological and mechanical properties of a novel injectable bone substitute, (6) improving strength of bone cements by incorporating reinforcing chemically modified fibres, (7) using adipose stem cells to stimulate osteogenesis, osteoinduction, and angiogenesis on calcium phosphates, (8) using glow discharge treatments to remove surface contaminants from biomedical materials to enhance cell attachment and improve bone generation, and (9) a review on how classically brittle hydroxyapatite based scaffolds can be improved by making fibre-hydroxyapatite composites, with detailed analysis of ceramic crack propagation mechanisms and its prevention via fibre incorporation in the hydroxyapatite.

Proliferation and Differentiation of MC3T3-E1 Cells on Calcium Phosphate/Chitosan Coatings

2008 ◽  
Vol 87 (7) ◽  
pp. 650-654 ◽  
Author(s):  
J. Wang ◽  
J. de Boer ◽  
K. de Groot
Keyword(s):  
Calcium Phosphate ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Attachment ◽  
Bone Sialoprotein ◽  
Differentiation Potential ◽  
Collagen Expression ◽  
Significant Difference ◽  
Proliferation And Differentiation ◽  
Proliferative Ability

The incorporation of chitosan into electro-deposited calcium phosphate (CaP) coatings increases bone marrow stromal cell attachment. We hypothesized that such electrodeposited CaP/chitosan coatings can also enhance the proliferative ability and differentiation potential of osteoblasts. To verify this hypothesis, we cultured osteoblast-like MC3T3-E1 cells on these CaP coatings. It was found that MC3T3-E1 cells cultured on the electrodeposited CaP/chitosan coatings had cell proliferation rates higher than those on the electrodeposited CaP coatings. At the same time, both alkaline phosphatase activity and collagen expression were increased, and both bone sialoprotein and osteocalcin genes were up-regulated when MC3T3-E1 cells were cultured on the electrodeposited CaP/chitosan coatings. Additionally, within the range of selected chitosan concentrations in solution, no significant difference was found between the CaP/chitosan coatings. Our results suggest that the electrodeposited CaP/chitosan coatings are favorable to the proliferation and differentiation of MC3T3-E1 cells, which may endow them with great potential for future applications.

scholarly journals Chitosan/ biphasic calcium phosphate nanofibrous scaffolds for bone tissue engineering

2020 ◽  
pp. 11-17
Author(s):  
Truong Le Bich Tram Truong
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Late Stage ◽  
Biphasic Calcium Phosphate ◽  
Cell Attachment ◽  
The Novel ◽  
Osteogenic Cells ◽  
Nanofibrous Scaffolds

In this article, chitosan/biphasic calcium phosphate (CS/BCP)nanofibers were prepared by electrospinning. From the culture of osteogenic cells, the biocompatibility of CS/BCP nanofibrous substrates was identified and increased by the photocrosslinking. The enhancement in cell attachment and proliferation was caused by the improvement in nanofibers’ mechanical properties. The biocompatibility to osteoblasts was also promoted with the content of BCP. The osteogenic differentiation in early, middle and late stage was encouraged by the addition of BCP on nanofibrous substrates. The CS/BCP nanofibers were highly specific to osteogenic cells, revealed by difficulties in the growth of non-osteogenic cells on this composite nanofibrous scaffold. The novel nanofibrous scaffolds showed great potential in the tissue engineering of bones.

Cell Behaviour of Calcium Phosphate Bone Cement Modified with a Protein-Based Foaming Agent

2005 ◽  
Vol 284-286 ◽  
pp. 117-120 ◽  
Author(s):  
Elisabeth Engel ◽  
L. Asin ◽  
J.A. Delgado ◽  
C. Aparicio ◽  
Josep A. Planell ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Cell Proliferation ◽  
Calcium Phosphate ◽  
Cell Attachment ◽  
Cell Behaviour ◽  
Foaming Agent ◽  
Calcium Phosphate Cements ◽  
New Methods ◽  
Calcium Phosphate Bone Cement

Calcium phosphate cements (CPC) are being applied as bone regeneration materials. New methods are being developed to create macroporosity, in order enhance the angiogenesis, bone colonization and biodegradation of the material. In this study the effect of the incorporation of albumen as foaming agent in an a-tricalcium phosphate (a-TCP) cement, in terms of the surface roughness and in vitro cell response, was studied. The addition of albumen to CPC cements increased the surface roughness of the cements. Cell cultures, using MG63 osteoblasts, were performed and showed that cell attachment was not affected by the presence of albumen. However, cell proliferation was significantly increased in the albumen-containing cements.

scholarly journals Development of magnesium calcium phosphate biocement for bone regeneration

2010 ◽  
Vol 7 (49) ◽  
pp. 1171-1180 ◽  
Author(s):  
Junfeng Jia ◽  
Huanjun Zhou ◽  
Jie Wei ◽  
Xin Jiang ◽  
Hong Hua ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Cell Attachment ◽  
Setting Time ◽  
Molar Ratio ◽  
Dihydrogen Phosphate ◽  
Good Ability ◽  
Degradation Ratio ◽  
Close Proximity ◽  
Proliferation And Differentiation ◽  
Fast Dissolution

Magnesium calcium phosphate biocement (MCPB) with rapid-setting characteristics was fabricated by using the mixed powders of magnesium oxide (MgO) and calcium dihydrogen phosphate (Ca(H 2 PO 4 ) 2 ·H 2 O). The results revealed that the MCPB hardened after mixing the powders with water for about 7 min, and the compressive strength reached 43 MPa after setting for 1 h, indicating that the MCPB had a short setting time and high initial mechanical strength. After the acid–base reaction of MCPB containing MgO and Ca(H 2 PO 4 ) 2 ·H 2 O in a molar ratio of 2 : 1, the final hydrated products were Mg 3 (PO 4 ) 2 and Ca 3 (PO 4 ) 2 . The MCPB was degradable in Tris–HCl solution and the degradation ratio was obviously higher than calcium phosphate biocement (CPB) because of its fast dissolution. The attachment and proliferation of the MG 63 cells on the MCPB were significantly enhanced in comparison with CPB, and the alkaline phosphatase activity of MG 63 cells on the MCPB was significantly higher than on the CPB at 7 and 14 days. The MG 63 cells with normal phenotype spread well on the MCPB surfaces, and were attached in close proximity to the substrate, as seen by scanning electron microscopy (SEM). The results demonstrated that the MCPB had a good ability to support cell attachment, proliferation and differentiation, and exhibited good cytocompatibility.

In Vitro Biocompatibility Testing of Collagen-Calcium Phosphate Composites Using Human Bone Derived Osteoblasts

1998 ◽  
Vol 550 ◽  
Author(s):  
A.C. Lawson ◽  
M. Oyama ◽  
M.E. Emerton ◽  
M.J.O. Francis ◽  
A.H.R.W. Simpson ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Calcium Phosphate ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Transforming Growth Factor ◽  
Cell Attachment ◽  
Octacalcium Phosphate ◽  
Histochemical Staining ◽  
Human Bone

AbstractHuman bone derived osteoblasts were cultured on collagen-calcium phosphate composites. The ability of the substrates to support cell attachment, proliferation and bone formation was assessed using histochemical staining for alkaline phosphatase activity and immunolocalisation of transforming growth factor- β1and type 1 collagen. The effect of calcium phosphate phase and crystal size was investigated and the calcified samples compared with uncalcified collagen. Osteoblasts adhere to the collagen-calcium phosphate composites and express a mature osteoblast phenotype in vitro. Cell adhesion was greater on unmineralised collagen than on the mineralised composites, however, these cells were less differentiated. The presence of larger crystals seemed to have a detrimental effect on the cells, reducing proliferation and alkaline phosphatase activity. There was no discernible difference between the effect of hydroxyapatite and octacalcium phosphate on the cells.

scholarly journals Cellular Performance Comparison of Biomimetic Calcium Phosphate Coating and Alkaline-Treated Titanium Surface

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaohua Yu ◽  
Mei Wei
Keyword(s):  
Calcium Phosphate ◽  
Titanium Surface ◽  
Cell Attachment ◽  
Substrate Surface ◽  
Calcium Phosphate Coating ◽  
Osteoblastic Cell ◽  
Phosphate Coating ◽  
Proliferation And Differentiation ◽  
The Impact

The influence of biomimetic calcium phosphate coating on osteoblasts behaviorin vitrois not well established yet. In this study, we investigated the behavior of osteoblastic rat osteosarcoma 17/2.8 cells (ROS17/2.8) on two groups of biomaterial surfaces: alkaline-treated titanium surface (ATT) and biomimetic calcium phosphate coated ATT (CaP). The cell attachment, proliferation, differentiation, and morphology on these surfaces were extensively evaluated to reveal the impact of substrate surface on osteoblastic cell responses. It was found that the ROS17/2.8 cells cultured on the ATT surface had higher attachment and proliferation rates compared to those on the CaP surface. Our results also showed that the calcium phosphate coatings generated in this work have an inhibiting effect on osteoblast adhesion and further influenced the proliferation and differentiation of osteoblast compared to the ATT surfacein vitro. Cells on the ATT surface also exhibited a higher alkaline phosphatase activity than on the CaP surface after two weeks of culture. Immunofluorescence staining and scanning electron microscopy results showed that the cells adhered and spread faster on the ATT surface than on the CaP surface. These results collectively suggested that substrate surface properties directly influence cell adhesion on different biomaterials, which would result in further influence on the cell proliferation and differentiation.

Calcium Phosphates as Fillers in Struvite Cements

2005 ◽  
Vol 284-286 ◽  
pp. 161-164 ◽  
Author(s):  
F.C.M. Driessens ◽  
M.G. Boltong ◽  
R. Wenz ◽  
J. Meyer
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphates ◽  
Ammonium Phosphate ◽  
Magnesium Ammonium Phosphate ◽  
Setting Times ◽  
Magnesium Ammonium ◽  
Series Of Experiments ◽  
Concentrated Solution ◽  
Magnesium Compound ◽  
Phosphate Filler

Struvite or magnesium ammonium phosphate MgNH4PO4 has been proposed as active component in setting surgical cements. The usual formulation is one in which the magnesium component in the powder is either magnesium hydrogen phosphate trihydrate or trimagnesium phosphate or a mixture of these two compounds. As the cement liquid a concentrated solution of diammonium phosphate is taken. To make the cement attractive as a bone substitute material a calcium phosphate filler is generally incorporated. Thus such materials are a type of pseudo calcium phosphate cements. This study was intended to find out which calcium phosphate and which magnesium compound are the most suitable. In the first series of experiments a mixture of 12 g Mg3(PO4)2 and 4 g MgHPO4.3H2O was used as the magnesium component in the powder. To that powder 30 g of either precipitated hydroxyapatite PHA or CaHPO4 or CaHPO4.H2O or b-TCP or a-TCP was added. The cement liquid was a 3.5 M solution of (NH4)2HPO4. At specific liquid/powder ratios L/P suitable setting times were obtained for the different formulations. However, the compressive strengths after immersion of the cements in 0.9% saline solution at 37°C varied over a large range. The best formulation was that with a-TCP which reached a compressive strength of 57 MPa after 18 h of immersion. In the second series of experiments 20 g of Mg3(PO4)2 was used as the magnesium component in the powder. Again 30 g of either of the above mentioned calcium phosphates was used as filler and again a 3.5 M solution of (NH4)2HPO4 was used as the cement liquid. At the appropriate L/P ratios the respective setting times were longer than in the first series of experiments but all five formulations appeared to result in good compressive strengths varying from 41 MPa for the formulation with b-TCP to 67 MPa for the formulation with PHA. In the third series of experiments 30 g a-TCP was taken as the calcium phosphate in the powder. As magnesium components mixtures of Mg3(PO4)2.8H2O and MgHPO4.3H2O and Mg3(PO4)2 were used. Again the cement liquid consisted of a 3.5 M solution of (NH4)2HPO4. The formulations with Mg3(PO4)2.8H2O had the shortest setting times and the lowest compressive strengths, whereas those with Mg3(PO4)2 had the longest setting times and the highest compressive strengths. Therefore, it is advantageous to use Mg3(PO4)2 as the magnesium component.

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