Activation of microglia associated with lentiviral transduction: A semiautomated method of assessment

The use of portfolios as an assessment tool probably is unlikely common in Asian countries. Portfolios is commonly considered as a compilation of students’ work for a certain period of time. In fact, the portfolios may show the originality of and the nature of students’ learning improvement. In this paper, the writer will discuss portfolios as a method of assessment in English as Second Language or English as Foreign Language (ESL/EFL) classroom context from teacher’s perspective. This paper is designed with this format order: firstly to explain about the degree of portfolios method to assess content, skills and knowledge in the teach-ing-learning program in the ESL/EFL context, to correlate portfolios system to model of curriculum planning and policy context. Secondly, to discuss the strengths and weaknesses of portfolios method from a theoretical perspective, and analyse the validity, reliability, and fairness. Thirdly, to specify the association of portfolios method and the types of assessment and the correlation of portfolios method with assessment for learning. Fourthly, to evaluate the degree of portfolios method of assessment promoting learning of the knowledge, skills, and abilities related to my teaching experience.Keywords : Portfolios, Assessment, Curriculum, Learning, Knowledge, Skills.

Download Full-text

A New Method of Assessment of the Degree of Anthropogenic Transformation of Suburban Woodlands

Сибирский экологический журнал ◽

10.15372/sej20160514 ◽

2016 ◽

Keyword(s):

New Method ◽

Anthropogenic Transformation ◽

Method Of Assessment

Download Full-text

Method of assessment of coal mines managers in the labor safety

Geo-Technical mechanics ◽

10.15407/geotm2019.149.077 ◽

2019 ◽

pp. 77-88

Author(s):

D.A. Nosal ◽

◽

V.H. Shevchenko ◽

Keyword(s):

Coal Mines ◽

Labor Safety ◽

Method Of Assessment

Download Full-text

Faculty Opinions recommendation of Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1760957.2713054 ◽

2010 ◽

Author(s):

Caroline Owen ◽

Martin Knolle

Keyword(s):

Alveolar Macrophages ◽

Lentiviral Transduction ◽

Pulmonary Alveolar Macrophages

Download Full-text

Efficient lentiviral transduction method to gene modify cord blood CD8+ T cells for cancer therapy applications

Molecular Therapy — Methods & Clinical Development ◽

10.1016/j.omtm.2021.03.015 ◽

2021 ◽

Vol 21 ◽

pp. 357-368

Author(s):

Vania Lo Presti ◽

Annelisa M. Cornel ◽

Maud Plantinga ◽

Ester Dünnebach ◽

Jurgen Kuball ◽

...

Keyword(s):

T Cells ◽

Cancer Therapy ◽

Cord Blood ◽

Cd8 T Cells ◽

Lentiviral Transduction

Download Full-text

Sarcoma IL-12 overexpression facilitates NK cell immunomodulation

Scientific Reports ◽

10.1038/s41598-021-87700-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Mary Jo Rademacher ◽

Anahi Cruz ◽

Mary Faber ◽

Robyn A. A. Oldham ◽

Dandan Wang ◽

...

Keyword(s):

Immune Response ◽

Cell Lines ◽

Nk Cell ◽

Autologous Tumor ◽

Murine Models ◽

Lentiviral Transduction ◽

Ifn Γ ◽

Human Sarcoma

AbstractInterleukin-12 (IL-12) is an inflammatory cytokine that has demonstrated efficacy for cancer immunotherapy, but systemic administration has detrimental toxicities. Lentiviral transduction eliciting IL-12-producing human sarcoma for autologous reintroduction provides localized delivery for both innate and adaptive immune response augmentation. Sarcoma cell lines and primary human sarcoma samples were transduced with recombinant lentivirus engineering expression of human IL-12 (hu-IL-12). IL-12 expressing sarcomas were assessed in vitro and in vivo following implantation into humanized NSG and transgenic human IL-15 expressing (NSG.Tg(Hu-IL-15)) murine models. Lentiviral transduction (LV/hu-IL-12) of human osteosarcoma, Ewing sarcoma and rhabdomyosarcoma cell lines, as well as low-passage primary human sarcomas, engendered high-level expression of hu-IL-12. Hu-IL-12 demonstrated functional viability, eliciting specific NK cell-mediated interferon-γ (IFN-γ) release and cytotoxic growth restriction of spheroids in vitro. In orthotopic xenograft murine models, the LV/hu-IL-12 transduced human sarcoma produced detectable IL-12 and elicited an IFN-γ inflammatory immune response specific to mature human NK reconstitution in the NSG.Tg(Hu-IL-15) model while restricting tumor growth. We conclude that LV/hu-IL-12 transduction of sarcoma elicits a specific immune reaction and the humanized NSG.Tg(Hu-IL-15) xenograft, with mature human NK cells, can define in vivo anti-tumor effects and systemic toxicities. IL-12 immunomodulation through autologous tumor transduction and reintroduction merits exploration for sarcoma treatment.

Download Full-text

Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol

Blood ◽

10.1182/blood-2003-07-2414 ◽

2004 ◽

Vol 103 (10) ◽

pp. 3710-3716 ◽

Cited By ~ 61

Author(s):

Peter A. Horn ◽

Kirsten A. Keyser ◽

Laura J. Peterson ◽

Tobias Neff ◽

Bobbie M. Thomasson ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Gene Transfer ◽

Peripheral Blood ◽

Blood Cells ◽

Lentiviral Vectors ◽

Large Animal ◽

Hematopoietic Stem ◽

Lentiviral Transduction ◽

Mobilized Peripheral Blood

Abstract The use of lentiviral vectors for the transduction of hematopoietic stem cells has evoked much interest owing to their ability to stably integrate into the genome of nondividing cells. However, published large animal studies have reported highly variable gene transfer rates of typically less than 1%. Here we report the use of lentiviral vectors for the transduction of canine CD34+ hematopoietic repopulating cells using a very short, 18-hour transduction protocol. We compared lentiviral transduction of hematopoietic repopulating cells from either stem cell factor (SCF)– and granulocyte-colony stimulating factor (G-CSF)–primed marrow or mobilized peripheral blood in a competitive repopulation assay in 3 dogs. All dogs engrafted rapidly within 9 days. Transgene expression was detected in all lineages (B cells, T cells, granulocytes, and red blood cells as well as platelets) indicating multilineage engraftment of transduced cells, with overall long-term marking levels of up to 12%. Gene transfer levels in mobilized peripheral blood cells were slightly higher than in primed marrow cells. In conclusion, we show efficient lentiviral transduction of canine repopulating cells using an overnight transduction protocol. These results have important implications for the design of stem cell gene therapy protocols, especially for those diseases in which the maintenance of stem cells in culture is a major limitation.

Download Full-text

Conceiving Socioscientific Issues in STEM Lessons from Science Education Research and Practice

Education Sciences ◽

10.3390/educsci11050238 ◽

2021 ◽

Vol 11 (5) ◽

pp. 238

Author(s):

Silvia Alcaraz-Dominguez ◽

Mario Barajas

Keyword(s):

Science Education ◽

Learning Strategies ◽

Teaching And Learning ◽

Socioscientific Issues ◽

Educational Systems ◽

Group Discussions ◽

Inquiry Based Learning ◽

Teaching And Learning Strategies ◽

Assessment Of Student Learning ◽

Method Of Assessment

This paper aims to elicit the different conceptions of Socioscientific Issues (SSI) in formal, non-tertiary STEM lessons. An examination of recent publications in the field of science education was conducted to elicit the most common conceptions of SSI as per the components of STEM lessons, namely: purpose, contents, teaching and learning strategies and assessment. As for purpose, the conceptions elicited have been organized in terms of contributing to citizenship goals, or to scientific competence. As for contents, it was found that SSI are related both to knowledge of science and knowledge about science and linked to skills such as argumentation. In terms of teaching and learning strategies, SSI are mainly associated with Inquiry-Based Learning; and with student engagement techniques such as dilemmas and group discussions. Lastly, performance assessment of student learning processes and results is typical when SSI are conceived as a method of assessment of STEM lessons. This conception sets up strong foundations for the design and evaluation of innovative SSI teaching. It shall also help to open new lines of research establishing connections among applications of SSI in different subjects, cultural contexts and educational systems.

Download Full-text