Comparative analysis of early-stage adsorption and biofilm formation of thermoacidophilic archaeon Acidianus manzaensis YN-25 on chalcopyrite and pyrite surfaces

2020 ◽  
Vol 163 ◽  
pp. 107744 ◽  
Author(s):  
Guirong Su ◽  
Xiaotao Deng ◽  
Liang Hu ◽  
Loganathan Praburaman ◽  
Hui Zhong ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Biofilm Formation ◽  
Early Stage ◽  
Acidianus Manzaensis

scholarly journals Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

2021 ◽  
Author(s):  
Martin Teren ◽  
Ekaterina Shagieva ◽  
Lucie Vondrakova ◽  
Jitka Viktorova ◽  
Viviana Svarcova ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Biofilm Formation ◽  
Early Stage ◽  
Luxs Gene

scholarly journals Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

2021 ◽  
Vol 9 (2) ◽  
pp. 385 ◽  
Author(s):  
Zongmin Liu ◽  
Lingzhi Li ◽  
Qianwen Wang ◽  
Faizan Ahmed Sadiq ◽  
Yuankun Lee ◽  
...  
Keyword(s):  
Network Analysis ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Molecular Mechanisms ◽  
Early Stage ◽  
Bifidobacterium Longum ◽  
Regulation Of Gene Expression ◽  
Interaction Network ◽  
Structural Development ◽  
Sequencing Analysis

Biofilm formation has evolved as an adaptive strategy for bacteria to cope with harsh environmental conditions. Currently, little is known about the molecular mechanisms of biofilm formation in bifidobacteria. A time series transcriptome sequencing analysis of both biofilm and planktonic cells of Bifidobacterium longum FGSZY16M3 was performed to identify candidate genes involved in biofilm formation. Protein–protein interaction network analysis of 1296 differentially expressed genes during biofilm formation yielded 15 clusters of highly interconnected nodes, indicating that genes related to the SOS response (dnaK, groS, guaB, ruvA, recA, radA, recN, recF, pstA, and sufD) associated with the early stage of biofilm formation. Genes involved in extracellular polymeric substances were upregulated (epsH, epsK, efp, frr, pheT, rfbA, rfbJ, rfbP, rpmF, secY and yidC) in the stage of biofilm maturation. To further investigate the genes related to biofilm formation, weighted gene co-expression network analysis (WGCNA) was performed with 2032 transcript genes, leading to the identification of nine WGCNA modules and 133 genes associated with response to stress, regulation of gene expression, quorum sensing, and two-component system. These results indicate that biofilm formation in B. longum is a multifactorial process, involving stress response, structural development, and regulatory processes.

Low concentration of Tween-20 enhanced the adhesion and biofilm formation of Acidianus manzaensis YN-25 on chalcopyrite surface

Chemosphere ◽  
2021 ◽  
pp. 131403
Author(s):  
Guirong Su ◽  
Shuzhen Li ◽  
Xiaotao Deng ◽  
Liang Hu ◽  
Loganathan Praburaman ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Tween 20 ◽  
Low Concentration ◽  
Acidianus Manzaensis

scholarly journals THE INFLUENCE OF NANO-SILVER ON FORMATION OF MICROBIAL BIOFILMS IN CASES OF TRAUMATIC LESION OF THE AUXILIARY APPARATUS OF THE EYE

2020 ◽  
Vol 4 ◽  
pp. 32-37
Author(s):  
Oksana Petrenko ◽  
Maryna Dranko ◽  
Victoriia Holubnycha ◽  
Larysa Hrytsai
Keyword(s):  
Biofilm Formation ◽  
Early Stage ◽  
Silver Concentration ◽  
Ionic Form ◽  
Bacterial Strains ◽  
Nano Silver ◽  
Traumatic Lesion ◽  
Traumatic Lesions ◽  
Acinetobacter Spp ◽  
Colloid Silver

Pyoinflammatory complications remain an acute problem in the post-operative period of traumatic lesions of the auxiliary apparatus of the eye (AAE). Silver both in the ionic form and in composition of chemical compounds is highly toxic for microorganisms, and as a result, it shows bactericidal effect to many bacterial strains, including gram-negative microorganisms. The peculiarity of AgNPs is efficiency of influence on the wide array of microorganisms, significant anti-biofilm effect and absence of resistance reaction. The aim of the research. To study the influence of the colloidal nano silver on formation of biofilms by microorganisms discharged from the wounds of patients with traumatic lesions of the auxiliary apparatus of the eye. Materials and methods. During 2018-2019, we examined 60 patients with traumatic lesions of the auxiliary apparatus of the eye. For evaluation of the influence of colloid nano silver solution on the processes of formation of the biofilm, we selected microorganisms which were cultured most frequently (Staphylococcus aureus, Acinetobacter spp., Klebsiella ozenae) from the patients. Results. The obtained data suggest that colloid nano silver inhibits efficiently formation of biofilms at the early stages (initiation, the 0 day of incubation) of their formation by all the three microorganisms, and the degree of inhibition of the biofilm formation did not depend on the silver concentration. The effect of colloid silver in the concentrations used by us at later stages of biofilm formation (the 3rd and the 7th day) with respect to К. ozenae is less efficient – the growth of cell biomass was observed (p≤0.05), and it did not depend on the silver concentration. At the same time, the effect of the colloid nano silver on S. aureus and Acinetobacter spp. on the 3rd and the 7th days was more efficient than at the early stage (p≤0.05). Conclusions. Nanoparticles of colloid silver are an efficient means to combat biofilms, as well as to prevent their formation.

A Comparative Analysis of Early Stage Diabetes Prediction using Machine Learning and Deep Learning Approach

2021 ◽  
Author(s):  
Md Abu Rumman Refat ◽  
Md. Al Amin ◽  
Chetna Kaushal ◽  
Mst Nilufa Yeasmin ◽  
Md Khairul Islam
Keyword(s):  
Machine Learning ◽  
Deep Learning ◽  
Comparative Analysis ◽  
Early Stage ◽  
Learning Approach ◽  
Diabetes Prediction

scholarly journals Pseudomonas aeruginosa Requires the DNA-Specific Endonuclease EndA To Degrade Extracellular Genomic DNA To Disperse from the Biofilm

2019 ◽  
Vol 201 (18) ◽  
Author(s):  
Kathryn E. Cherny ◽  
Karin Sauer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Genomic Dna ◽  
Early Stage ◽  
Content Type ◽  
Biofilm Dispersion ◽  
Biofilm Structure ◽  
First Time ◽  
Biofilm Matrix ◽  
Dispersed Cells

ABSTRACT The dispersion of biofilms is an active process resulting in the release of planktonic cells from the biofilm structure. While much is known about the process of dispersion cue perception and the subsequent modulation of the c-di-GMP pool, little is known about subsequent events resulting in the release of cells from the biofilm. Given that dispersion coincides with void formation and an overall erosion of the biofilm structure, we asked whether dispersion involves degradation of the biofilm matrix. Here, we focused on extracellular genomic DNA (eDNA) due to its almost universal presence in the matrix of biofilm-forming species. We identified two probable nucleases, endA and eddB, and eddA encoding a phosphatase that were significantly increased in transcript abundance in dispersed cells. However, only inactivation of endA but not eddA or eddB impaired dispersion by Pseudomonas aeruginosa biofilms in response to glutamate and nitric oxide (NO). Heterologously produced EndA was found to be secreted and active in degrading genomic DNA. While endA inactivation had little effect on biofilm formation and the presence of eDNA in biofilms, eDNA degradation upon induction of dispersion was impaired. In contrast, induction of endA expression coincided with eDNA degradation and resulted in biofilm dispersion. Thus, released cells demonstrated a hyperattaching phenotype but remained as resistant to tobramycin as biofilm cells from which they egress, indicating EndA-dispersed cells adopted some but not all of the phenotypes associated with dispersed cells. Our findings indicate for the first time a role of DNase EndA in dispersion and suggest weakening of the biofilm matrix is a requisite for biofilm dispersion. IMPORTANCE The finding that exposure to DNase I impairs biofilm formation or leads to the dispersal of early stage biofilms has led to the realization of extracellular genomic DNA (eDNA) as a structural component of the biofilm matrix. However, little is known about the contribution of intrinsic DNases to the weakening of the biofilm matrix and dispersion of established biofilms. Here, we demonstrate for the first time that nucleases are induced in dispersed Pseudomonas aeruginosa cells and are essential to the dispersion response and that degradation of matrix eDNA by endogenously produced/secreted EndA is required for P. aeruginosa biofilm dispersion. Our findings suggest that dispersing cells mediate their active release from the biofilm matrix via the induction of nucleases.

scholarly journals The impact of Paenibacillus polymyxa HY96-2 luxS on biofilm formation and control of tomato bacterial wilt

2019 ◽  
Vol 103 (23-24) ◽  
pp. 9643-9657 ◽  
Author(s):  
Jincui Yi ◽  
Daojing Zhang ◽  
Yuejuan Cheng ◽  
Jingjing Tan ◽  
Yuanchan Luo
Keyword(s):  
Bacterial Wilt ◽  
Late Stage ◽  
Biofilm Formation ◽  
Early Stage ◽  
Regulatory Gene ◽  
Paenibacillus Polymyxa ◽  
Deletion Strain ◽  
Biocontrol Efficacy ◽  
The Impact ◽  
Tomato Bacterial Wilt

Abstract The focus of this study was to investigate the effects of luxS, a key regulatory gene of the autoinducer-2 (AI-2) quorum sensing (QS) system, on the biofilm formation and biocontrol efficacy against Ralstonia solanacearum by Paenibacillus polymyxa HY96-2. luxS mutants were constructed and assayed for biofilm formation of the wild-type (WT) strain and luxS mutants of P. polymyxa HY96-2 in vitro and in vivo. The results showed that luxS positively regulated the biofilm formation of HY96-2. Greenhouse experiments of tomato bacterial wilt found that from the early stage to late stage postinoculation, the biocontrol efficacy of the luxS deletion strain was the lowest with 50.70 ± 1.39% in the late stage. However, the luxS overexpression strain had the highest biocontrol efficacy with 75.66 ± 1.94% in the late stage. The complementation of luxS could restore the biocontrol efficacy of the luxS deletion strain with 69.84 ± 1.09% in the late stage, which was higher than that of the WT strain with 65.94 ± 2.73%. Therefore, we deduced that luxS could promote the biofilm formation of P. polymyxa HY96-2 and further promoted its biocontrol efficacy against R. solanacearum.

Qualitative comparative analysis in corporate governance research: a systematic literature review of applications

2019 ◽  
Vol 19 (4) ◽  
pp. 717-734 ◽  
Author(s):  
Nicola Cucari
Keyword(s):  
Corporate Governance ◽  
Comparative Analysis ◽  
Literature Review ◽  
Systematic Literature Review ◽  
Early Stage ◽  
Qualitative Comparative Analysis ◽  
Content Type ◽  
Stage Of Development ◽  
Governance Practices ◽  
Complex Phenomena

Purpose The purpose of this paper is to provide comprehensive mapping of qualitative comparative analysis (QCA) applications in business and management research and to examine the sub-fields of corporate governance research in this context. Design/methodology/approach Through a systematic literature review of 22 articles, the paper describes and analyses how QCA is used in the corporate governance field, what can be learned from the methodology’s implementation in corporate governance studies and why authors justify its use. Findings The findings highlight that QCA in corporate governance is still at an early stage of development. The paper encourages governance scholars to use this method to transform QCA from a niche into a mainstream method because it is appropriate for understanding both complex phenomena of social reality and issues of corporate governance that require an approach able to capture configurations of conditions, asymmetric patterns and equifinal explanations. Originality/value This is the first complete overview of the existing literature concerning QCA’s application in corporate governance research and reveals implications for its future use. In this way, it extends the previous work on QCA’s benefits to management researchers and other critical reviews of applications in QCA. This study encourages scholars to renew their understanding of corporate governance issues through a new analysis method that can help to discover conceptual and empirical relations among case-oriented and variable-oriented analyses in terms of interrelations to examine corporate governance practices holistically.

scholarly journals Non-Destructive Monitoring of P. fluorescens and S. epidermidis Biofilm under Different Media by Fourier Transform Infrared Spectroscopy and Other Corroborative Techniques

Coatings ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 930
Author(s):  
Marco Consumi ◽  
Kamila Jankowska ◽  
Gemma Leone ◽  
Claudio Rossi ◽  
Alessio Pardini ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Early Stage ◽  
Deionized Water ◽  
The Other ◽  
Ftir Technique ◽  
Low Sensitivity ◽  
Non Destructive ◽  
Peculiar Behavior

In the present study, the early stage of bacteria biofilm formation has been studied as a function of different nutrients. Infrared spectra of Pseudomonas fluorescens (PF) and Staphylococcus epidermidis (SE), on germanium ATR crystal, were collected under deionized water H2O, phosphate buffered solution (PBS) and PBS with glucose (PBS-G). In H2O, protein bands of PF increased while, no difference in PBS and PBS-G were observed until 135 min. SE strain showed a low sensitivity to PBS composition starting to expose proteins on surfaces after 120 min. SE shows a low polysaccharides increase in H2O while, in bare and enriched PBS their intensity increases after 120 and 75 min. in PBS and PBS-G respectively. PF exhibits a peculiar behavior in H2O where the saccharide bands increased strongly after 100 min, while under all the other conditions, the intensity of polysaccharide bands increased up to the plateau probably because the layer of the biofilm exceeded the penetration capability of FTIR technique. All data suggest that, under lack of nutrients, both the bacteria tend to firmly anchor themselves to the support using proteins.

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