Antimicrobial resistance in biofilms: Exploring marine actinobacteria as a potential source of antibiotics and biofilm inhibitors

Antimicrobial resistance represents one of the most relevant threats to global public health and food security, affecting anyone, of any age, in any country and is responsible for longer hospital stays, higher medical costs and increased mortality. Resistant microorganisms are present in humans, animals, food and the environment, and, therefore, the One Health approach is very promising to counteract antimicrobial resistance, since human health and animal health are connected to each other and with the environment and the latter a potential source of resistant microorganisms. In this context, the need for novel antimicrobial drugs has stimulated the exploration of plant products as a source of novel phytotherapeutics able to reverse antimicrobial resistance when used in combination with conventional antibiotic drugs.

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Protease Inhibitors from Marine Actinobacteria as a Potential Source for Antimalarial Compound

PLoS ONE ◽

10.1371/journal.pone.0090972 ◽

2014 ◽

Vol 9 (3) ◽

pp. e90972 ◽

Cited By ~ 31

Author(s):

L. Karthik ◽

Gaurav Kumar ◽

Tarun Keswani ◽

Arindam Bhattacharyya ◽

S. Sarath Chandar ◽

...

Keyword(s):

Protease Inhibitors ◽

Marine Actinobacteria ◽

Potential Source

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Microbial and Antimicrobial Resistance Profiles of Microbiota in Common Carps (Cyprinus carpio) from Aquacultured and Wild Fish Populations

Animals ◽

10.3390/ani11040929 ◽

2021 ◽

Vol 11 (4) ◽

pp. 929

Author(s):

Modestas Ruzauskas ◽

Julija Armalytė ◽

Eglė Lastauskienė ◽

Rita Šiugždinienė ◽

Irena Klimienė ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Bacterial Species ◽

Fish Farming ◽

Wild Fish ◽

Resistant Bacteria ◽

Fish Ponds ◽

Opportunistic Pathogens ◽

Microbial Composition ◽

Potential Source ◽

Growing Conditions

In this study we analyzed differences in microbial composition and antimicrobial resistance profiles in common carp living in two different environments: fish ponds, where carp have been kept under the same growing conditions over the last 50 years, and from the wild. The results demonstrated that wild fish carry a great variety of bacterial species (448 species with a prevalence of at least 0.01% from the total number of reads). Aquacultured individuals harbored 2.56 times fewer species in their gut. Significant microbial differences were observed in all taxonomic ranks, including bacterial classes and phyla. Besides bacterial variety, it was determined that aquacultured fish harbored more bacteria that are considered pathogens or opportunistic pathogens, such as Moraxellaceae, Flavobacteriaceae, and Staphylococcaceae. The frequency of antimicrobial resistance in bacterial indicators was more common in aquacultured fish than in wild fish, therefore fish farming may be a potential source of environmental contamination with antimicrobial resistant bacteria.

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A review of antimicrobial resistance in imported foods

Canadian Journal of Microbiology ◽

10.1139/cjm-2021-0234 ◽

2021 ◽

Author(s):

Dongyun Jung ◽

Beverly J Morrison ◽

Joseph E. Rubin

Keyword(s):

Antimicrobial Resistance ◽

Food Products ◽

International Travel ◽

Resistant Bacteria ◽

New Delhi ◽

Medical Sciences ◽

Potential Source ◽

Surveillance Programs ◽

Imported Foods

Antimicrobial resistance is one of the most serious threats to the medical sciences. The food supply is recognized as a potential source of resistant bacteria, leading to the development of surveillance programs targeting primarily poultry, pork and beef. These programs are limited in scope not only in the commodities tested, but also in the organisms targeted (Escherichia coli, Salmonella and Campylobacter); consequently, neither the breadth of food products available nor the organisms which may harbour clinically relevant and/or mobile resistance genes are identified. Furthermore, there is an inadequate understanding of how the international trade in food products contributes to the global dissemination of resistance. This is despite the recognized role of international travel in dissemination disseminating antimicrobial-resistant organisms (ARO), notably the New Delhi Metallo-betalactamase. An increasing number of studies describing ARO in a variety of imported foods will be summarized in this review.

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The incidence and antimicrobial resistance of Arcobacter species in animal and poultry meat samples at slaughterhouses in Iran

Iranian Journal of Microbiology ◽

10.18502/ijm.v12i6.5027 ◽

2020 ◽

Author(s):

Sepideh Khodamoradi ◽

Ramin Abiri

Keyword(s):

Antimicrobial Resistance ◽

Broiler Chickens ◽

Broiler Chicken ◽

Poultry Meat ◽

Source Of Infection ◽

Potential Source ◽

Food Borne ◽

Arcobacter Butzleri ◽

Pcr Method ◽

Meat Samples

Background and Objectives: Arcobacter species are food-borne and zoonotic enteropathogens. Defined breakpoints for the investigation of antimicrobial resistance of Arcobacter are missing. Materials and Methods: The study was performed to investigate the incidence and antimicrobial resistance of Arcobacter species in animals and poultry meat samples procured from slaughterhouses in Iran. To investigate the prevalence of antimi- crobial resistance, samples were collected from cattle (n=100), sheep (n=100), goat (n=100), broiler chicken (n=100), turkey (n=100) and quail (n=100). Arcobacter isolates of meat samples were isolated, investigated by PCR method and antibiotic resistance was also investigated. The susceptibility was assessed by Kirby-Bauer disc diffusion. Results: The results showed that 52 samples (8.66%) were positive for Arcobacter spp. The most prevalence were observed in broiler chickens (26%, n=26 samples), quail (13%, n=13 samples), turkey (8%, n=8), cattle (3%, n=3), sheep (1%, n=1) and goat (1%, n=1). Arcobacter butzleri had highest prevalence among Arcobacter species. All the isolates showed sensitiv- ity to gentamicin, streptomycin and tetracycline. Conclusion: Poultry meat is a potential source of infection with Arcobacter that must be considered in slaughterhouses in Iran. Arcobacter species showed sensitivity for a broad spectrum of antibiotics that can be used during infection with Arco- bacter species.

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How SIMS microscopy can be used in medicine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100132649 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1602-1603

Author(s):

Philippe Fragu

Keyword(s):

Mass Spectrometry ◽

Biomedical Applications ◽

Secondary Ion Mass Spectrometry ◽

Chemical Elements ◽

Biological Applications ◽

The Past ◽

Potential Source ◽

Secondary Ion ◽

Chemical Integrity ◽

Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

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