scholarly journals The incidence and antimicrobial resistance of Arcobacter species in animal and poultry meat samples at slaughterhouses in Iran

2020 ◽  
Author(s):  
Sepideh Khodamoradi ◽  
Ramin Abiri
Keyword(s):  
Antimicrobial Resistance ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Poultry Meat ◽  
Source Of Infection ◽  
Potential Source ◽  
Food Borne ◽  
Arcobacter Butzleri ◽  
Pcr Method ◽  
Meat Samples

Background and Objectives: Arcobacter species are food-borne and zoonotic enteropathogens. Defined breakpoints for the investigation of antimicrobial resistance of Arcobacter are missing. Materials and Methods: The study was performed to investigate the incidence and antimicrobial resistance of Arcobacter species in animals and poultry meat samples procured from slaughterhouses in Iran. To investigate the prevalence of antimi- crobial resistance, samples were collected from cattle (n=100), sheep (n=100), goat (n=100), broiler chicken (n=100), turkey (n=100) and quail (n=100). Arcobacter isolates of meat samples were isolated, investigated by PCR method and antibiotic resistance was also investigated. The susceptibility was assessed by Kirby-Bauer disc diffusion. Results: The results showed that 52 samples (8.66%) were positive for Arcobacter spp. The most prevalence were observed in broiler chickens (26%, n=26 samples), quail (13%, n=13 samples), turkey (8%, n=8), cattle (3%, n=3), sheep (1%, n=1) and goat (1%, n=1). Arcobacter butzleri had highest prevalence among Arcobacter species. All the isolates showed sensitiv- ity to gentamicin, streptomycin and tetracycline. Conclusion: Poultry meat is a potential source of infection with Arcobacter that must be considered in slaughterhouses in Iran. Arcobacter species showed sensitivity for a broad spectrum of antibiotics that can be used during infection with Arco- bacter species.

Multiple-Antibiotic Resistance of Enterococcus faecalis and Enterococcus faecium from Cecal Contents in Broiler Chicken and Turkey Flocks Slaughtered in Canada and Plasmid Colocalization of tetO and ermB Genes

2011 ◽  
Vol 74 (10) ◽  
pp. 1639-1648 ◽  
Author(s):  
CINDY-LOVE TREMBLAY ◽  
ANN LETELLIER ◽  
SYLVAIN QUESSY ◽  
MARTINE BOULIANNE ◽  
DANIELLE DAIGNAULT ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Enterococcus Faecalis ◽  
Resistance Genes ◽  
Enterococcus Faecium ◽  
Broiler Chicken ◽  
Multidrug Resistant ◽  
Poultry Meat ◽  
Antimicrobial Resistance Genes ◽  
High Level ◽  
Level Resistance

This study was conducted to characterize the antimicrobial resistance determinants and investigate plasmid colocalization of tetracycline and macrolide genes in Enterococcus faecalis and Enterococcus faecium from broiler chicken and turkey flocks in Canada. A total of 387 E. faecalis and E. faecium isolates were recovered from poultry cecal contents from five processing plants. The percentages of resistant E. faecalis and E. faecium isolates, respectively, were 88.1 and 94% to bacitracin, 0 and 0.9% to chloramphenicol, 0.7 and 14.5% to ciprofloxacin, 72.6 and 80.3% to erythromycin, 3.7 and 41% to flavomycin, 9.6 and 4.3% (high-level resistance) to gentamicin, 25.2 and 17.1% (high-level resistance) to kanamycin, 100 and 94% to lincomycin, 0 and 0% to linezolid, 2.6 and 20.5% to nitrofurantoin, 3 and 27.4% to penicillin, 98.5 and 89.7% to quinupristin-dalfopristin, 7 and 12.8% to salinomycin, 46.7 and 38.5% (high-level resistance) to streptomycin, 95.6 and 89.7% to tetracycline, 73 and 75.2% to tylosin, and 0 and 0% to vancomycin. One predominant multidrug-resistant phenotypic pattern was identified in both E. faecalis and E. faecium (bacitracin, erythromycin, lincomycin, quinupristin-dalfopristin, tetracycline, and tylosin). These isolates were further examined by PCR and sequencing for the genes encoding their antimicrobial resistance. Various combinations of vatD, vatE, bcrR, bcrA, bcrB, bcrD, ermB, msrC, linB, tetM, and tetO genes were detected, and ermB, tetM, and bcrB were the most common antimicrobial resistance genes identified. For the first time, plasmid extraction and hybridization revealed colocalization of tetO and ermB genes on a ca. 11-kb plasmid in E. faecalis isolates, and filter mating experiments demonstrated its transferability. Results indicate that the intestinal enterococci of healthy poultry, which can contaminate poultry meat at slaughter, could be a reservoir for quinupristin-dalfopristin, bacitracin, tetracycline, and macrolide resistance genes.

scholarly journals 623. Antimicrobial Resistance in Non-Typhoidal Salmonella from Retail Poultry Meat by Antibiotic Usage-related Production Claims—Pennsylvania, 2008–2017

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S289-S289
Author(s):  
Xin Yin ◽  
Nkuchia M M’ikanatha ◽  
Lisa Dettinger ◽  
Melinda Johnston ◽  
William Eckroth ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antibiotic Usage ◽  
Poultry Meat ◽  
Health Concern ◽  
Whole Genome Sequencing Data ◽  
Sequencing Data ◽  
Antimicrobial Drugs ◽  
Salmonella Infections ◽  
Drug Classes ◽  
Meat Samples

Abstract Background Antimicrobial-resistant (AMR) nontyphoidal Salmonella infections are a public health concern. Injudicious use of antimicrobials fuels emergence of resistance. The National Antimicrobial Resistance Monitoring System (NARMS) tracks AMR in Salmonella from humans, animals and foods. There is limited evidence regarding antimicrobial use in food animals and AMR bacteria in retail meat. Methods We reviewed antimicrobial susceptibility and whole-genome sequencing data from 320 Salmonella isolated from poultry meat in Pennsylvania as part of NARMS activities. Salmonella strains were isolated from 3,481 samples purchased from randomly selected retail outlets during 2008–2017. Antibiotic usage claims on meat packages were used to compare AMR Salmonella from conventional and antibiotic-free/organic (Abx-free) samples. Genetic mechanisms for AMR were investigated in a subset of isolates. Results The prevalence of Salmonella in conventional poultry meat 10.2% (280/2,733) was significantly higher than the prevalence in poultry meat labeled as Abx-free (5.3%, 40/748; P < 0.0001). Salmonella from conventional poultry meat was more likely to be resistant to 3 or more drugs (55.0%, 154/280) compared with poultry meat labeled as Abx-free (27.5%, 11/40; P = 0.0011). Salmonella from conventional poultry exhibited significantly higher resistance to 4 drug classes including β-lactams (P = 0.006) (figure). One hundred isolates from conventional poultry meat and 8 isolates from antibiotic-free/organic samples harbored a gene conferring resistance to the β-lactam class; 24.3% (68/280) of isolates from conventional and 7.5% (3/40) of isolates from Abx-free samples (P = 0.0145) contained the extended-spectrum β-lactamase (ESBL) gene blaCMY-2. Conclusion Meat samples from conventionally-raised poultry were more likely to be contaminated with AMR Salmonella strains and have genes that reduce the effectiveness of antimicrobial drugs recommended for treatment of severe infections. Contamination of poultry with AMR Salmonella strains is concerning as is the presence of genes that decrease the power of critical antibiotics such as β-lactams. These findings highlight the importance of judicious use of antibiotics in food-producing animals. Disclosures All authors: No reported disclosures.

High Level of Antimicrobial Resistance in Campylobacter jejuni Isolated from Broiler Chickens in Estonia in 2005 and 2006

2007 ◽  
Vol 70 (8) ◽  
pp. 1940-1944 ◽  
Author(s):  
MATI ROASTO ◽  
KADRIN JUHKAM ◽  
TERJE TAMME ◽  
ARI HÖRMAN ◽  
LIIDIA HÄKKINEN ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Animal Origin ◽  
Campylobacter Coli ◽  
National Veterinary Institute ◽  
Food Of Animal Origin ◽  
Meat Samples ◽  
High Level

The development of antimicrobial resistance in Campylobacter jejuni and Campylobacter coli is a matter of increasing concern. Because campylobacteriosis is transmitted to humans usually via food of animal origin, the presence of antimicrobial-resistant campylobacters in broiler chickens has important public health implications. The aim of our study was to analyze resistance patterns of C. jejuni isolated from fecal samples collected at a large Estonian chicken farm, from cecal contents collected at slaughterhouses, and from meat samples collected at the retail establishments in 2005 and 2006. A total of 131 C. jejuni isolates were collected over a 13-month period and tested by the broth microdilution VetMIC method (National Veterinary Institute, Uppsala, Sweden) to determine the MICs of various antimicrobials. Resistance to one or more antimicrobials was detected in 104 (79.4%) of the 131 isolates. High proportions of the isolates were resistant to enrofloxacin (73.3%) and nalidixic acid (75.6%). Multidrug resistance (resistance to three or more unrelated antimicrobials) was detected in 36 isolates (27.5%), all of which were resistant to enrofloxacin. Multidrug resistance was significantly associated with enrofloxacin resistance (P &lt; 0.01), and the use of enrofloxacin may select for multiresistant strains.

scholarly journals Antimicrobial resistance prevalence of pathogenic and commensal Escherichia coli in food-producing animals in Belgium

2014 ◽  
Vol 83 (5) ◽  
pp. 225-233 ◽  
Author(s):  
I. Chantziaras ◽  
J. Dewulf ◽  
F. Boyen ◽  
B. Callens ◽  
P. Butaye
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Susceptibility Testing ◽  
Commensal Bacteria ◽  
The Other ◽  
E Coli ◽  
Veal Calves ◽  
Veal Calf

In this article, detailed studies on antimicrobial resistance to commensal E. coli (in pigs, meatproducing bovines, broiler chickens and veal calves) and pathogenic E. coli (in pigs and bovines) in Belgium are presented for 2011. Broiler chicken and veal calf isolates of commensal E. coli demonstrated higher antimicrobial resistance prevalence than isolates from pigs and bovines. Fifty percent of E. coli isolates from broiler chickens were resistant to at least five antimicrobials, whereas sixty-one percent of bovine E. coli isolates were susceptible to all antimicrobials tested. On the other hand, bovine pathogenic E. coli isolates showed an extended resistance profile with more than half of the isolates being resistant to ten or more antimicrobials. The results are not significantly different from the results from previously published studies on commensal bacteria in pigs in Belgium, although different methodologies of sampling and susceptibility testing were used.

scholarly journals THE INFLUENCE OF IONIZING RADIATION ON THE THERMOPHYSICAL PROPERTIES OF MEAT FROM THE BROILER CHICKENS WITH DIFFERENT STRESS RESISTANCE

2019 ◽  
Vol 4 (1) ◽  
pp. 4-8
Author(s):  
Roza T. Timakova ◽  
Sergey L. Tikhonov ◽  
Natal’ya V. Tikhonova ◽  
Sergey V. Shikhalev ◽  
Leonid S. Kudryashov
Keyword(s):  
Ionizing Radiation ◽  
Thermophysical Properties ◽  
Broiler Chickens ◽  
Radiation Treatment ◽  
Meat Product ◽  
Meat Products ◽  
Poultry Meat ◽  
Thermal Processes ◽  
Capacity Coefficient ◽  
Meat Samples

The studies on the influence of radiation treatment of carcasses from the stress-resistant and stress-sensitive broiler chickens on the thermophysical properties of raw meat are presented. An increase in thermal diffusivity of meat from the stress-resistant poultry by 24.7 % and 54.7 % after radiation treatment of carcasses with ionizing radiation doses of 9 kGy and 12 kGy, respectively, was established. In meat from the stress-sensitive poultry, this figure increased by 33.3 % and 35.8 % compared to the untreated carcasses.It is shown that radiation treatment of carcasses by applied doses increased the thermal conductivity coefficient of meat from the stressresistant poultry by 5.3% and 7.0 %; in meat from the stress-sensitive poultry, this figure increased by 2.0 and 6.2 times compared to meat from the carcasses not exposed to radiation. At the same time, the value of the heat capacity coefficient was reduced. The irradiated poultry meat samples accumulate energy of ionizing radiation more intensively, which allows the intensification of the thermal processes occurring at various stages of meat product production. Treatment of meat from the stress-resistant poultry with ionizing radiation can reduce the amount of meat with non-traditional autolysis due to changes in its functionaltechnological properties. The results of the research should be taken into account in technological processes in the production of meat products with non-traditional autolysis.

scholarly journals Detection of Blood Protozoa Infecting Broiler Chicken Farms in Tanjung Gunung Village, District Jombang

2019 ◽  
Vol 3 (1) ◽  
pp. 5
Author(s):  
Marchelia Arifiandani ◽  
Endang Suprihati ◽  
Wiwik Misaco Yuniarti ◽  
Nunuk Dyah Retno Lastuti ◽  
Poedji Hastutiek ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Broiler Chicken ◽  
Life Cycles ◽  
Purposive Sampling ◽  
Potential Source ◽  
Result Show

Broiler chicken farms could be a part of potential source infection Leucocytozoonosis and Plasmodiosis disease due to the death of livestock. Broiler farms in Tanjung Gunung, District Jombang near the fields, water, and trees. This could be increase the population of vectors infection Leucocytozoonosis and Plasmodiosis. The main purpose of this researchis to detect Leucocytozoonosis and Plasmodiosis infections on broiler farms in Tnjung Gunung Village, District Jombang using 50 broiler chickens by Purposive Sampling  of 2 bredeers. The result show rhat there are 6 positive samples infected Leucocytozoonosis, while Plasmodiosis infection could not be fond. Need cage sanitation to break life cycles of vector Leucocytozoonosis and Plasmodiosis.

Prevalence and antimicrobial resistance of Arcobacter butzleri and Arcobacter cryaerophilus isolates from retail meat in Lower Silesia region, Poland

2015 ◽  
Vol 18 (1) ◽  
pp. 63-69 ◽  
Author(s):  
I. Zacharow ◽  
J. Bystroń ◽  
E. Wałecka-Zacharska ◽  
M. Podkowik ◽  
J. Bania
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Chicken Meat ◽  
Significant Incidence ◽  
Arcobacter Butzleri ◽  
Contaminated Food ◽  
Retail Meat ◽  
Human Poisoning ◽  
Meat Samples ◽  
Lower Silesia

AbstractArcobacter butzleriandA. cryaerophilusare considered potential foodborne pathogens. Consumption ofArcobacter-contaminated food is regarded the most likely source of human poisoning. We investigated the prevalence and antimicrobial resistance ofArcobacterisolates in 210 retail meat samples. Seventy-nineA. butzleriand 6A. cryaerophiluswere isolated from pork, beef and chicken meat. Incidence ofA. butzleriwas found to be the highest in chicken meat (83%). Less ofA. butzleriwas isolated from beef (16%) and pork (14%). Most of theA. butzleriisolates were resistant to β-lactams, like ampicillin (85%), amoxicillin with clavulonic acid (63%), cefotaxime (66%) and macrolides, i.e., erythromycin (62%). In contrast, all except oneA. cryaerophilusisolates were susceptible to erythromycin. Tetracycline and aminoglycosides showed the highest efficacy againstA. butzleriandA. cryaerophilussince almost 80% of their population was susceptible to these agents. All, except oneA. cryaerophilusand the majority ofA. butzleriisolates (70%) were susceptible to fluoroquinolones. The incidence of multiresistant isolates was found in forty two (53%)A. butzleri, and one (16%)A. cryaerophilusisolates. EightA. butzleriisolates were resistant to all antimicrobials tested. These results indicate significant incidence of potential foodborne zoonotic agents, i.e.A. butzleriandA. cryaerophilusincluding multiresistant isolates in retail meat in Poland.

Sign in / Sign up

Export Citation Format

Share Document