Genetic Manipulation of the Fusarium fujikuroi Fusarin Gene Cluster Yields Insight into the Complex Regulation and Fusarin Biosynthetic Pathway

The biosynthetic pathway of the uridine-derived nucleoside antibiotic A-94964 was proposed via in silico analysis coupled with gene deletion experiments.

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Apicidin F: Characterization and Genetic Manipulation of a New Secondary Metabolite Gene Cluster in the Rice Pathogen Fusarium fujikuroi

PLoS ONE ◽

10.1371/journal.pone.0103336 ◽

2014 ◽

Vol 9 (7) ◽

pp. e103336 ◽

Cited By ~ 40

Author(s):

Eva-Maria Niehaus ◽

Slavica Janevska ◽

Katharina W. von Bargen ◽

Christian M. K. Sieber ◽

Henning Harrer ◽

...

Keyword(s):

Gene Cluster ◽

Secondary Metabolite ◽

Genetic Manipulation ◽

Fusarium Fujikuroi ◽

Secondary Metabolite Gene Cluster

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Biosynthesis of Fusarubins Accounts for Pigmentation of Fusarium fujikuroi Perithecia

Applied and Environmental Microbiology ◽

10.1128/aem.00823-12 ◽

2012 ◽

Vol 78 (12) ◽

pp. 4468-4480 ◽

Cited By ~ 109

Author(s):

Lena Studt ◽

Philipp Wiemann ◽

Karin Kleigrewe ◽

Hans-Ulrich Humpf ◽

Bettina Tudzynski

Keyword(s):

Secondary Metabolites ◽

Gene Cluster ◽

Biosynthetic Pathway ◽

Polyketide Synthase ◽

Biological Function ◽

Fusarium Fujikuroi ◽

Diverse Group ◽

Fruiting Bodies ◽

Content Type ◽

Encoding Gene

ABSTRACTFusarium fujikuroiproduces a variety of secondary metabolites, of which polyketides form the most diverse group. Among these are the highly pigmented naphthoquinones, which have been shown to possess different functional properties for the fungus. A group of naphthoquinones, polyketides related to fusarubin, were identified inFusariumspp. more than 60 years ago, but neither the genes responsible for their formation nor their biological function has been discovered to date. In addition, although it is known that the sexual fruiting bodies in which the progeny of the fungus develops are darkly colored by a polyketide synthase (PKS)-derived pigment, the structure of this pigment has never been elucidated. Here we present data that link the fusarubin-type polyketides to a defined gene cluster, which we designatefsr, and demonstrate that the fusarubins are the pigments responsible for the coloration of the perithecia. We studied their regulation and the function of the single genes within the cluster by a combination of gene replacements and overexpression of the PKS-encoding gene, and we present a model for the biosynthetic pathway of the fusarubins based on these data.

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Concordant evolution of trichothecene 3-O-acetyltransferase and an rDNA species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi

Microbiology ◽

10.1099/mic.0.27435-0 ◽

2005 ◽

Vol 151 (2) ◽

pp. 509-519 ◽

Cited By ~ 24

Author(s):

Takeshi Tokai ◽

Makoto Fujimura ◽

Hirokazu Inoue ◽

Takayuki Aoki ◽

Kunihiro Ohta ◽

...

Keyword(s):

Biosynthetic Pathway ◽

Magnaporthe Grisea ◽

Gibberella Fujikuroi ◽

Fusarium Fujikuroi ◽

Biosynthesis Pathway ◽

Functional Identification ◽

The Core ◽

Species Phylogeny ◽

Fusarium Asiaticum ◽

Insight Into

The cereal pathogen Fusarium graminearum species complex (e.g. Fusarium asiaticum, previously referred to as F. graminearum lineage 6) produces the mycotoxin trichothecene in infected grains. The fungus has a gene for self-defence, Tri101, which is responsible for 3-O-acetylation of the trichothecene skeleton in the biosynthetic pathway. Recently, trichothecene non-producers Fusarium oxysporum and Fusarium fujikuroi (teleomorph Gibberella fujikuroi) were shown to have both functional (Tri201) and non-functional (pseudo-Tri101) trichothecene 3-O-acetyltransferase genes in their genome. To gain insight into the evolution of the trichothecene genes in Gibberella species, the authors examined whether or not other (pseudo-)biosynthesis-related genes are found near Tri201. However, sequence analysis of a 12 kb region containing Tri201 did not result in identification of additional trichothecene (pseudo-)genes in F. oxysporum. In a further attempt to find other trichothecene (pseudo-)genes from the non-producer, the authors examined whether or not the non-trichothecene genes flanking the ends of the core trichothecene gene cluster (i.e. the Tri5 cluster) comprise a region of synteny in Gibberella species. However, it was not possible to isolate trichothecene (pseudo-)genes from F. oxysporum (in addition to the previously identified pseudo-Tri101), because synteny was not observed for this region in F. asiaticum and F. oxysporum. In contrast to this unsuccessful identification of additional trichothecene (pseudo-)genes in the non-producer, a functional trichothecene 3-O-acetyltransferase gene could be identified in fusaria other than Gibberella: Fusarium decemcellulare and Fusarium solani; and in an ascomycete from a different fungal genus, Magnaporthe grisea. Together with the recent functional identification of Saccharomyces cerevisiae ScAYT1, these results are suggestive of a different evolutionary origin for the trichothecene 3-O-acetyltransferase gene from other biosynthesis pathway genes. The phylogeny of the 3-O-acetyltransferase was mostly concordant with the rDNA species phylogeny of these ascomycetous fungi.

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NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax

Molecules ◽

10.3390/molecules26030767 ◽

2021 ◽

Vol 26 (3) ◽

pp. 767

Author(s):

Kamar Hamade ◽

Ophélie Fliniaux ◽

Jean-Xavier Fontaine ◽

Roland Molinié ◽

Elvis Otogo Nnang ◽

...

Keyword(s):

Stress Response ◽

Osmotic Stress ◽

Biosynthetic Pathway ◽

Potential Role ◽

Plant Secondary Metabolites ◽

Wild Type ◽

Osmotic Stress Response ◽

Transgenic Flax ◽

Insight Into

Lignans, phenolic plant secondary metabolites, are derived from the phenylpropanoid biosynthetic pathway. Although, being investigated for their health benefits in terms of antioxidant, antitumor, anti-inflammatory and antiviral properties, the role of these molecules in plants remains incompletely elucidated; a potential role in stress response mechanisms has been, however, proposed. In this study, a non-targeted metabolomic analysis of the roots, stems, and leaves of wild-type and PLR1-RNAi transgenic flax, devoid of (+) secoisolariciresinol diglucoside ((+) SDG)—the main flaxseed lignan, was performed using 1H-NMR and LC-MS, in order to obtain further insight into the involvement of lignan in the response of plant to osmotic stress. Results showed that wild-type and lignan-deficient flax plants have different metabolic responses after being exposed to osmotic stress conditions, but they both showed the capacity to induce an adaptive response to osmotic stress. These findings suggest the indirect involvement of lignans in osmotic stress response.

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Whole Genome Sequencing of Fusarium fujikuroi Provides Insight into the Role of Secretory Proteins and Cell Wall Degrading Enzymes in Causing Bakanae Disease of Rice

Frontiers in Plant Science ◽

10.3389/fpls.2017.02013 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 9

Author(s):

Bishnu M. Bashyal ◽

Kirti Rawat ◽

Sapna Sharma ◽

Deepika Kulshreshtha ◽

S. Gopala Krishnan ◽

...

Keyword(s):

Cell Wall ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Fusarium Fujikuroi ◽

Secretory Proteins ◽

Whole Genome ◽

Cell Wall Degrading Enzymes ◽

Bakanae Disease ◽

Insight Into

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A Bacterial Microcompartment Is Used for Choline Fermentation byEscherichia coli536

Journal of Bacteriology ◽

10.1128/jb.00764-17 ◽

2018 ◽

Vol 200 (10) ◽

Cited By ~ 12

Author(s):

Taylor I. Herring ◽

Tiffany N. Harris ◽

Chiranjit Chowdhury ◽

Sujit Kumar Mohanty ◽

Thomas A. Bobik

Keyword(s):

Electron Microscopy ◽

Heart Disease ◽

Gene Cluster ◽

Transcriptional Regulator ◽

Human Gut ◽

Content Type ◽

E Coli ◽

Bacterial Microcompartment ◽

New Type ◽

Insight Into

ABSTRACTBacterial choline degradation in the human gut has been associated with cancer and heart disease. In addition, recent studies found that a bacterial microcompartment is involved in choline utilization byProteusandDesulfovibriospecies. However, many aspects of this process have not been fully defined. Here, we investigate choline degradation by the uropathogenEscherichia coli536. Growth studies indicatedE. coli536 degrades choline primarily by fermentation. Electron microscopy indicated that a bacterial microcompartment was used for this process. Bioinformatic analyses suggested that the choline utilization (cut) gene cluster ofE. coli536 includes two operons, one containing three genes and a main operon of 13 genes. Regulatory studies indicate that thecutXgene encodes a positive transcriptional regulator required for induction of the maincutoperon in response to choline supplementation. Each of the 16 genes in thecutcluster was individually deleted, and phenotypes were examined. ThecutX,cutY,cutF,cutO,cutC,cutD,cutU, andcutVgenes were required for choline degradation, but the remaining genes of thecutcluster were not essential under the conditions used. The reasons for these varied phenotypes are discussed.IMPORTANCEHere, we investigate choline degradation inE. coli536. These studies provide a basis for understanding a new type of bacterial microcompartment and may provide deeper insight into the link between choline degradation in the human gut and cancer and heart disease. These are also the first studies of choline degradation inE. coli536, an organism for which sophisticated genetic analysis methods are available. In addition, thecutgene cluster ofE. coli536 is located in pathogenicity island II (PAI-II536) and hence might contribute to pathogenesis.

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