Vitrification Changes The Fatty Acids Profile of Zebrafish Ovarian Follicles At Different Developmental Stages

The selection and diffusion in the poultry sector of hybrid breeds able to produce higher amount of meat and eggs, led over time to the erosion of genetic resources. One of the strategies that can be applied in order to stem such phenomenon, concerns the valorization of the animal products, meat or eggs, obtained from indigenous poultry breeds. Therefore, the aim of this study is to characterize the qualitative aspects of eggs obtained from the Nera Atriana hen, an Italian indigenous laying hen characteristic of the Abruzzo region, making a direct comparison with a commercial hybrid reared in the same environment and applying the same feeding protocol. The trial was conducted for a period of 5 months (from March to July 2019), in which 6 egg samplings were performed (one at the beginning and additional 5 on a monthly basis); each sampling involved the collection of 15 eggs per group of animals for a total of 90 eggs per genotype. Eggs were specifically subjected to evaluations of the physical parameters, including the yolk color, and analyses aimed at determining the content of total lipids, cholesterol, and β-carotene. In addition to this, the fatty acids profile and the electrophoretic pattern of the globular proteins of the yolk were characterized. Eggs from hybrid hens were characterized by higher total weight with significantly higher values specifically associated to albumen and yolk weight and to the albumen percentage. In the eggs obtained from the local hen, higher relative percentages were instead found in the quantity of shell and yolk; furthermore, in the same samples was observed a higher yolk redness, a cholesterol concentration tending to be lower although not significant (p = 0.0521), and a greater amount of saturated fatty acids which could justify a lower susceptibility of the food to oxidative processes following transformation with heat treatments. With regard to the fatty acid composition, differences were specifically observed for palmitic (C16:0) and palmitoleic (C16:1 cis-9) acids that showed higher relative percentages in eggs from local breed, and for the oleic acid (C18:1 cis-9) which was more represented in eggs from commercial hens. In conclusion, some interesting nutritional features, in a preliminary way, have been highlighted that could lead the consumer to prefer these “niche” products over those obtained from commercial hybrids.

Download Full-text

Steroid production by amphibian (Rana nigromaculata) ovarian follicles at different developmental stages

Journal of Experimental Zoology ◽

10.1002/jez.1402600109 ◽

1991 ◽

Vol 260 (1) ◽

pp. 66-73 ◽

Cited By ~ 18

Author(s):

Hyuk B. Kwon ◽

Han H. Choi ◽

Ryun S. Ahn ◽

Yong D. Yoon

Keyword(s):

Developmental Stages ◽

Ovarian Follicles ◽

Steroid Production ◽

Rana Nigromaculata

Download Full-text

Performance, carcass traits and meat quality of lambs fed with different roughage:concentrate ratios associated with variable physically effective neutral detergent fibre content

The Journal of Agricultural Science ◽

10.1017/s0021859621000459 ◽

2021 ◽

pp. 1-11

Author(s):

L. de O. Queiroz ◽

A. M. Barbosa ◽

G. B. Mourão ◽

M. A. Fonseca ◽

L. F. B. Pinto ◽

...

Keyword(s):

Fatty Acids ◽

Particle Size ◽

Meat Quality ◽

Average Daily Gain ◽

Carcass Traits ◽

Faecal Excretion ◽

Neutral Detergent Fibre ◽

Fatty Acids Profile ◽

Colour Parameter

Abstract This study evaluated the effect of roughage:concentrate (R:C) ratio associated with a variable particle size of physically effective neutral detergent fibre (peNDF8) in the forage (Tifton-85 hay) on the performance, carcass traits and meat quality of lambs. Seventy-two 4-month-old, non-castrated Santa Ines male lambs (23.5 ± 2.32 kg BW) were distributed in a completely randomized design, in a 2 × 2 factorial arrangement [two peNDF8 hay particle sizes (13 and 6 mm) and two R:C ratios (700:300 and 500:500 g/kg DM total)]. DMI, DM, NFC and TDN digestibility's, N-intake and N-faecal excretion were affected by the R:C ratio (P < 0.05). However, the N-retained was not affected by the studied variables (P > 0.05). It was observed an interaction (P < 0.05) between the peNDF8 and R:C ratios for final BW, average daily gain (ADG), colour parameters and pH 24 h. The lower roughage ratio provided greater (P < 0.05) concentrations of C14:1, C16:1–cis9, C18:1–cis9, ΣMUFA, Σn–6:Σn–3 and hypocholesterolemic/hypercholesterolemic index, enzymatic activity Δ9desaturase-C16 and -C18. Lambs fed a lower roughage diet had improved performance and feed efficiency, however, presented reduced polyunsaturated fatty acids (PUFA) concentrations in the meat, especially Σn–3 family. Higher roughage diet and larger peNDF8 particle size improved the concentrations of PUFA while decreased Σn–6:Σn–3 ratio in meat. Larger peNDF8 particle size associated with higher roughage proportion, have reduced animal performance however, it increased protein concentration, a* and C* colour parameter without affecting fatty acids profile of Longissimus lumborum muscle.

Download Full-text

Fat content and fatty acids profile of colostrum and milk of primitive Konik horses (Equus caballus gmelini Ant.) during six months of lactation

Journal of Dairy Research ◽

10.1017/s0022029908003336 ◽

2008 ◽

Vol 75 (3) ◽

pp. 302-309 ◽

Cited By ~ 15

Author(s):

Jan Pikul ◽

Jacek Wójtowski ◽

Romualda Danków ◽

Beata Kuczyńska ◽

Jacek Łojek

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Equus Caballus ◽

Fat Content ◽

Slight Effect ◽

Milk Samples ◽

Stage Of Lactation ◽

Fatty Acids Profile

The effect of the stage of lactation, the number of foals and age of the mare on changes in the fat content and fatty acid composition of colostrum and milk of primitive Konik horses was investigated. Colostrum and milk samples from 12 lactating mares were collected at the beginning of lactation, on the days 1 and 2 after foaling and then, starting from the first month of lactation, at 4-week intervals up to the sixth month of lactation. Significant differences were observed in fat content as well as the composition of some analysed fatty acids between colostrum and milk of mares of the Konik breed. The number of foalings and the age of mares did not have a statistically significant effect on the fat content in milk and had only a slight effect on the fatty acid composition. Milk produced by mares of the Konik breed is characterized by a considerable content of polyene fatty acids with 18 carbon atoms, a low ratio of n-6 fatty acids to n-3 fatty acids as well as low, highly advantageous values of atherogenic and thrombogenic indices.

Download Full-text

Effects of Dietary Supplementation with Different Polyunsaturated Fatty Acids on Expression of Genes Related to Somatotropic Axis Function in the Liver, Selected Blood Indicators, Milk Yield and Milk Fatty Acids Profile in Dairy Cows

Annals of Animal Science ◽

10.1515/aoas-2016-0019 ◽

2016 ◽

Vol 16 (4) ◽

pp. 1045-1058 ◽

Cited By ~ 9

Author(s):

Essa Dirandeh ◽

Armin Towhidi ◽

Zarbakht Ansari ◽

Saeeid Zeinoaldini ◽

Mehdi Ganjkhanlou

Keyword(s):

Fatty Acids ◽

Plasma Concentrations ◽

Dietary Supplementation ◽

Omega 3 ◽

Milk Fatty Acids ◽

Somatotropic Axis ◽

Fatty Acids Profile ◽

Expression Of Genes ◽

Omega 6 ◽

And Control

Abstract The objective of this study was to investigate whether dietary supplementation with different polyunsaturated fatty acids (PUFA s) affects expression of genes related to somatotropic axis and the plasma concentrations of insulin, glucose, non-esterified fatty acids (NEFA), beta hydroxyl butyrate acids (BHBA) and insulin-like growth factor 1 (IGF1) and milk fatty acids profile. Right after calving, Holstein cows (n=45) were randomly assigned to one of three diets supplemented with roasted whole soybean as a source of omega-6 PUFA (omega-6, n=15), linseed as a source of omega-3 PUFA (omega-3, n=15) or palm oil (control, n=15). Each cow was in the study over a period of 70 days. Blood samples were collected every two weeks from day 1 to 70 of lactation and plasma concentrations of insulin, glucose, NEFA, BHBA and IGF1 were determined. Liver samples were taken from a subset of 18 cows (6 per diet) at day 70 postpartum and hepatic mRNA level of total growth hormone-receptor 1A (GHR1A), insulin receptor (INSR), IGF1 and insulinlike growth factor binding protein (IGFBP2) was assessed. Experimental diets did not affect milk yield. Plasma glucose and insulin concentrations were greater for omega-3 treatment compared to omega-6 and control treatments. Cows fed diets enriched in omega-3 exhibited greater INSR and GHR1A mRNA expression, and a tendency for greater IGF1 mRNA expression in the liver compared to omega-6 and control cows. Plasma IGF1 concentration was significantly higher in omega-3 treatment compared with omega-6 and control treatments. Results of this study suggest that feeding omega-3 PUFA s during early postpartum couples with the somatotropic axis, leading to an increase in plasma IGF1 concentration in dairy cows.

Download Full-text

Adding palm oil to the diet of sheep alters fatty acids profile on yogurt: Benefits to consumers

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201720170468 ◽

2017 ◽

Vol 89 (3 suppl) ◽

pp. 2471-2478 ◽

Cited By ~ 4

Author(s):

ANDERSON E. BIANCHI ◽

ALEKSANDRO S. DA SILVA ◽

ANGELISA H. BIAZUS ◽

NEILA S.P.S. RICHARDS ◽

LUIS G. PELLEGRINI ◽

...

Keyword(s):

Fatty Acids ◽

Palm Oil ◽

Fatty Acids Profile

Download Full-text

Fatty Acids Profile, Physicalchemical Properties and Minerals with Quantify Indicador of Astrocaryum aculeatum Pulp Oil

Journal of Agricultural Science ◽

10.5539/jas.v9n12p352 ◽

2017 ◽

Vol 9 (12) ◽

pp. 352

Author(s):

Bernardo M. Linhares ◽

Ana Marcia D. C. Costa ◽

Herliana D. F. Abreu ◽

Ana Cristina G. Reis de Melo ◽

Pedro R. E. Ribeiro ◽

...

Keyword(s):

Fatty Acids ◽

Physicochemical Properties ◽

Unsaturated Fatty Acids ◽

Essential Fatty Acids ◽

Saturated Fatty Acids ◽

Fatty Acids Profile ◽

High Concentrations ◽

Astrocaryum Aculeatum ◽

Air Circulation ◽

Acid Index

The species Astrocaryum aculeatum (Arecaceae) is known in the Brazilian Amazon as tucumã, whose fruit is much appreciated by the population of the region, where its pulp, oleaginous, is the most consumed. Thus, the aim of this work was to perform a profile of fatty acids by GC-FID and minerals by ICP-OES of the oil of the pulp of the tucumã (A. aculeatum), as well as their physicochemical properties by 1H NMR. The fruits were collected in Alto Alegre city, Roraima, Brazil. These were taken to the laboratory, sanitized and removing your pulp, submitted to the oven with air circulation at 50 °C for 72 h, the dried pulps were milled and sieved between 20-40 mesh. The pulp oil extraction was realized in Soxhlet with hexane for 6 hours (yield of 54.7%). Were identified a total of 10 fatty acids, of these 23.8% are saturated fatty acids and 76.2% are unsaturated fatty acids: palmitic acid (10.4%), stearic acid (4.9%), oleic acid (64.2%), linoleic acid (11%) and linolenic acid (1%). The physicochemical properties have a pulp oil acid index of 0.31 mg KOH g-1, saponification of 190.39 mg KOH g-1, iodine index of 85.97 mg g-1. Minerals such as in their available forms K (70.05 mg L-1) Na (30.30 mg L-1), Ca (20.13 mg L-1) and P (20.07 mg L-1) were observed in high concentrations. The Amazon tucumã is an oleaginous that deserves our attention because it is composed of essential fatty acids that are beneficial to the human health.

Download Full-text