Data on ICP OES and emulsion stability of Bredemeyera floribunda root extract: Medicinal plant used by the Brazilian rural population to treat snakebites

Background: Developing new antimicrobial medicines is one of the strategic objectives of the global action plan adopted by the World Health Organization to tackle antimicrobial resistance. Objective: Considering the fact that natural products derived from medicinal plants are an important source for discovering new antimicrobial compounds, we investigated here the antimicrobial properties and the mode of action of various extracts from Fredolia aretioides, an endemic medicinal plant of Morocco and Algeria, and belonging to the Chenopodiaceae family. Methods: Various extracts from F. aretioides were prepared and evaluated for their antibacterial activity against six bacterial species, and their antifungal activity against five fungi species. Chemicalgenetic screens were performed using a collection of Saccharomyces cerevisiae viable haploid deletion mutants spanning diverse biological processes. Results: The diethyl ether extract of roots was found to be active against Citobacter freundii, with a MIC of 400 µg/ml. Hydro-methanol, methanol and residual extracts from aerial parts and roots were active against all five fungi species tested. Our results showed that residual extracts were the most effective against the fungi tested. Residual extract from aerial parts was more potent than the residual root extract, with IC50’s of 60 µg/ml and 440 µg/ml, respectively. Chemical-genetic analysis in S. cerevisiae revealed that residual extracts might affect fatty acid and sphingolipid biosynthesis. Conclusion: All these findings suggest that F. aretioides is a promising source for the isolation of novel antimicrobial agents with novel mechanisms of action against human pathogens.

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Anti-Inflammatory Properties of Bellevalia saviczii Root Extract and Its Isolated Homoisoflavonoid (Dracol) Are Mediated by Modification on Calcium Signaling

Molecules ◽

10.3390/molecules24183376 ◽

2019 ◽

Vol 24 (18) ◽

pp. 3376

Author(s):

Monica Savio ◽

Mohammed Farhad Ibrahim ◽

Chiara Scarlata ◽

Matteo Orgiu ◽

Giuseppe Accardo ◽

...

Keyword(s):

Medicinal Plant ◽

Western Blot ◽

Calcium Signaling ◽

Inflammatory Cytokines ◽

Nuclear Translocation ◽

Herbal Remedy ◽

Root Extract ◽

Cytokine Release ◽

Iraqi Kurdistan ◽

Anti Inflammatory

Bellevalia saviczii is a medicinal plant used as anti-rheumatic and anti-inflammatory herbal remedy in Iraqi-Kurdistan. The aim of this study was to evaluate the anti-inflammatory activity of its extract and the isolated homoisoflavonoid (Dracol) by studying the Ca2+-dependent NF-kB pathway. Nuclear translocation of p65 NF-kB subunit, as parameter of NF-kB activation, was visualized in human leukemic monocytes by immunofluorescence and Western blot analyses, after cell treatment with B. saviczii root extract or Dracol followed by Lipopolysaccharide stimulation. In parallel, Ca2+ signals responsible for NF-kB activation and levels of inflammatory cytokines were investigated. LPS-induced p65 translocation was evident in monocytes and both treatments, in particular that with Dracol, were able to counteract this activation. Intracellular Ca2+ oscillations were halted and the cytokine release reduced. These results confirm the traditional anti-inflammatory efficacy of B. saviczii and identify one of the molecules in the extract which appears to be responsible of this action.

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Pharmaco-chemical profiling of Desmodium gangeticum (L.) DC. with special reference to soil chemistry

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00356-7 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Prasobh K. Mohan ◽

T. P. Adarsh Krishna ◽

T. Senthil Kumar ◽

B. D. Ranjitha Kumari

Keyword(s):

Amino Acid ◽

Medicinal Plant ◽

Special Reference ◽

Dna Sequence ◽

Soil Chemistry ◽

Soil Analysis ◽

Ethanolic Extract ◽

Root Extract ◽

Chemical Profiling ◽

Query Coverage

Abstract Background Desmodium gangeticum (L.) DC. (Fabaceae) (DG) is a perennial non-climbing herb or shrub and folklore medicine, widely shows a large number of medicinal properties, as well as contains divergent bioactive compounds. Many of the herbal formulations contain this medicinal plant, which is considered as master of medicinal plant in Ayurveda. This study is an attempt to establish this plant material based on its pharmaco-chemical profiles with special reference to soil chemistry. The pharmaco-chemical features such as organoleptic, DNA sequence, physicochemical, proximate, phytochemical, UV, and FTIR profiling were carried out using standard techniques. Moreover, the ADME-PK properties of the selected molecules were established. Results The pharmaco-chemical features like organoleptic, DNA sequence, physicochemical, proximate, phytochemical, UV, and FTIR profiling, ADME-PK properties, and soil chemistry of D. gangeticum revealed its unique and diagnostic peculiarities. DNA barcoding showed that the sequence was 99.77% similar to D. gangeticum (KP094638) having 100% query coverage. The soil analysis revealed the presence of moderately high content of NPK and sufficient amount of all essential macro- and micronutrients (S, Fe, Mn, Cu, Zn, and B). The phytochemical profiling showed that the ethanolic extract of the aerial part contained glycoside, amino acid, phenols, alkaloids, flavonoids, and coumarins, while the ethanolic root extract of the plant revealed the presence of glycoside, amino acid, phenols, alkaloids, flavonoids, coumarins, and triterpenoids. FTIR results indicated that the plant extracts are mainly rich in phenolic derivatives. ADME-PK properties of pterocarpan such as gangetin (1a), gangetinin (1b), desmocarpin (1c), and desmodin (1d) were found to pass the Lipinski, Ghose, Veber, and Egan rules, supporting the drug-likeliness. Conclusion This is the first record of pharmaco-chemical profiling of D. gangeticum along with soil chemistry, and this information helps in the proper identification and future studies on this species. Graphic abstract

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Croton zambesicus Root Extract Exert Laxative Effect in Rats

Asian Journal of Biology ◽

10.9734/ajob/2020/v10i230103 ◽

2020 ◽

pp. 22-26

Author(s):

Jude E. Okokon ◽

Augustine I. L. Bassey ◽

Emmanuel E. Nyong ◽

Utibe A. Edem

Keyword(s):

Gastrointestinal Tract ◽

Medicinal Plant ◽

Experimental Models ◽

Root Extract ◽

Laxative Effect

Background: Croton zambesicus Muell Arg. (Euphorbiaceace) is a medicinal plant used traditionally in the treatment of some ailments. Aim: The root extract of Croton zambesicus was evaluated for effect on the gastrointestinal tract (GIT). Materials and Methods: The root extract of C. zambesicus (27-81 mg/kg) was investigated for laxative effect in rats using different experimental models to study its effect on the GIT. Results: The extract was found to significantly increase the frequency and output of faecal matters in rats in all the experimental models studied. Conclusion: The findings of this study show that the root extract of C. zambesicus has a significant laxative effect and this supports its use in ethnomedicine for the treatment of gastrointestinal tract disorders.

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The Root Extract of the Medicinal Plant Pelargonium sidoides Is a Potent HIV-1 Attachment Inhibitor

PLoS ONE ◽

10.1371/journal.pone.0087487 ◽

2014 ◽

Vol 9 (1) ◽

pp. e87487 ◽

Cited By ~ 40

Author(s):

Markus Helfer ◽

Herwig Koppensteiner ◽

Martha Schneider ◽

Stephanie Rebensburg ◽

Sara Forcisi ◽

...

Keyword(s):

Medicinal Plant ◽

Root Extract ◽

Pelargonium Sidoides ◽

Hiv 1

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Insecticidal and Antifeedant Activities of Malagasy Medicinal Plant (Cinnamosma sp.) Extracts and Drimane-Type Sesquiterpenes against Aedes aegypti Mosquitoes

Insects ◽

10.3390/insects10110373 ◽

2019 ◽

Vol 10 (11) ◽

pp. 373 ◽

Cited By ~ 6

Author(s):

Inocente ◽

Nguyen ◽

Manwill ◽

Benatrehina ◽

Kweka ◽

...

Keyword(s):

Medicinal Plant ◽

Aedes Aegypti ◽

Insect Pests ◽

Root Extract ◽

Mosquito Vectors ◽

Antifeedant Activity ◽

Root Extracts ◽

Modes Of Action ◽

Traditional Remedies ◽

Primary Driver

The overuse of insecticides with limited modes of action has led to resistance in mosquito vectors. Thus, insecticides with novel modes of action are needed. Secondary metabolites in Madagascan plants of the genus Cinnamosma (Canellaceae) are commonly used in traditional remedies and known to elicit antifeedant and toxic effects in insect pests. Here we test the hypothesis that extracts of Cinnamosma sp. enriched in drimane sesquiterpenes are toxic and/or antifeedant to the yellow fever mosquito Aedes aegypti. We show that the bark and root extracts, which contain a higher abundance of drimane sesquiterpenes compared to leaves, were the most efficacious. Screening isolated compounds revealed cinnamodial to be the primary driver of adulticidal activity, whereas cinnamodial, polygodial, cinnafragrin A, and capsicodendrin contributed to the larvicidal activity. Moreover, an abundant lactone (cinnamosmolide) in the root extract synergized the larvicidal effects of cinnamodial. The antifeedant activity of the extracts was primarily contributed to cinnamodial, polygodial, and cinnamolide. Parallel experiments with warburganal isolated from Warburgia ugandensis (Canellaceae) revealed that aldehydes are critical for—and a hydroxyl modulates—insecticidal activity. Our results indicate that plant drimane sesquiterpenes provide valuable chemical platforms for developing insecticides and repellents to control mosquito vectors.

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Identifikasi Dan Penetapan Kadar Flavonoid Total Ekstrak Akar Kuning (Fibraurea chloroleuca Miers)

Talenta Conference Series Tropical Medicine (TM) ◽

10.32734/tm.v1i3.269 ◽

2018 ◽

Vol 1 (3) ◽

pp. 095-098

Author(s):

Mauritz Pandapotan Marpaung ◽

Riska Choirunnisa Wahyuni

Keyword(s):

Medicinal Plants ◽

Qualitative Study ◽

Medicinal Plant ◽

Total Flavonoid ◽

Root Extract ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Absorbance Measurement ◽

Traditional Medicinal Plants

Akar kuning (Fibraurea chloroleuca Miers) merupakah salah satu tumbuhan obat tradisional yang digunakan oleh masyarakat untuk mengobati berbagai penyakit. Untuk menjadikan akar kuning sebagai tumbuhan obat yang sesuai standar, perlu dilakukan standardisasi. Salah satu standardisasi sediaan obat tradisional adalah penetapan kadar salah satu kandungan senyawa aktif dalam akar kuning. Penelitian ini bertujuan untuk mengidentifikasi kandungan flavonoid dan menentukan kadar flavonoid total ekstrak akar kuning. Penelitian ini menggunakan metode maserasi untuk memperoleh ekstrak kental akar kuning dengan pelarut etanol 70%. Untuk menetapkan kadar flavonoid total dari ekstrak akar kuning dilakukan uji kualitatif dan pengukuran absorbansi melalui spektrofotometri UV-Vis dengan larutan standar kuersetin. Hasil penelitian secara kualitatif menunjukkan bahwa akar kuning mengandung flavonoid setelah penambahan AlCl3 1% dengan kadar flavonoid total yang terkandung dalam ekstrak akar kuning melalui pengukuran spektrofotometri Uv-Vis pada panjang gelombang maksimum 442 nm adalah 0,31031 ± 0,013607%. Berdasarkan hasil di atas, maka dapat disimpulkan bahwa ekstrak akar kuning mengandung flavonoid dengan kadar sebesar 0,31031 ± 0,013607%. Akar kuning (Fibraurea chloroleuca Miers) is one of the traditional medicinal plants to treat various diseases. To prepare akar kuning as a medicinal plant according to the standard, standardization needs to be done. One of standardization parameter of traditional medicine is the determination of the content of one of the active compounds in akar kuning. This research aimed to identify flavonoid content and determine the total flavonoid content of akar kuning ekstrak. The study used maceration method with ethanol 70% to prepare crude extract of akar kuning. To determine the total flavonoid content of akar kuning extract, the qualitative study and absorbance measurement using UV-Vis Spectrophotometer with quercetin as standard were performed. The results of qualitative study showed that akar kuning contained flavonoids which evaluated by the addition of 1% AlCl3, with the total flavonoid content of akar kuning extract by UV-Vis spectrophotometry measurements at a maximum wavelength of 442 nm was 0.31031 ± 0.013607%. Based on the results above, it can be concluded that yellow root extract contains flavonoids with levels of 0.31031 ± 0.013607%

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