Comparative physiological and proteomic analysis of Arabidopsis thaliana revealed differential wound stress responses following the exposure to different LED light sources

Karuppanapandian, T., Rhee, S.-J., Kim, E.-J., Han, B. K., Hoekenga, O. A. and Lee, G. P. 2012. Proteomic analysis of differentially expressed proteins in the roots of Columbia-0 and Landsberg erecta ecotypes of Arabidopsis thaliana in response to aluminum-toxicity. Can. J. Plant Sci. 92: 1267–1282. Aluminum (Al) is phytotoxic when solubilized into Al3+ in acidic soils and represents a major constraint for crop production. The present study describes Al-stress responses in roots of Al-tolerant and Al-sensitive Arabidopsis ecotypes, Columbia-0 (Col-0) and Landsberg erecta (Ler), respectively. Comparative proteomic analysis was applied to plants grown in hydroponic solution culture under acidic pH (4.2) conditions. To investigate time-dependent responses, 6-d-old seedlings were treated with 30 µM AlCl3 for 24, 48, or 72 h; total proteins were prepared from roots and separated by two-dimensional gel electrophoresis (2-DE). From 2-DE analysis, were 600 proteins were inspected, 29 proteins were differentially responsive to Al-treatment. The 2-DE patterns were compared and differentially expressed proteins identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Analysis of protein expression patterns revealed that a set of proteins is functionally associated with tricarboxylic acid (TCA) cycle and glycolysis, reactive oxygen quenching and detoxification mechanism, and signal transduction pathways, etc., could play important roles in mediating plant response to Al in Arabidopsis ecotypes. Comparison of the changes in the protein profiles revealed that Al-stress increased Al-tolerance related proteins in Al-tolerant Col-0, but only generic stress responses occurred in Al-sensitive Ler. Specifically, Al up-regulated proteins such as alcohol dehydrogenase, monodehydroascorbate reductase, GTP-binding nuclear protein Ran-2, and leucine aminopeptidase in Col-0 but not in Ler.

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Design of Solar-Powered LED Road Lighting System

Light & Engineering ◽

10.33383/2017-077 ◽

2019 ◽

pp. 75-85

Author(s):

Canan Perdahci ◽

Hamdi Ozkan

Keyword(s):

High Pressure ◽

Renewable Energy Sources ◽

Junction Temperature ◽

Mercury Vapour ◽

Light Sources ◽

Street Lighting ◽

Led Light ◽

Road Lighting ◽

Lighting Systems ◽

High Pressure Sodium Lamp

Turkey is rich in terms of renewable energy sources and, therefore, is now encouraging the use of sustainable clean lighting systems in road applications. High pressure sodium lamp is the most widely used type in main roads, but other types of lamps such as mercury vapour lamps or metal halide lamps can be utilized for street lighting. Since it enables energy and money saving, LED light technology has replaced high pressure sodium lamps nowadays. Once solar power system (PV) is integrated with LED lamp for street lighting, the amount of saving and local impact might be enriched. LEDs used as light sources in road lighting luminaires with rising lumen values, decreasing junction temperature, higher colour rendering efficiency, longer lifetime have become more efficient than many light sources with the latest developments. Since the structure of the luminaires in which the LED light sources are used differs from that of the conventional light sources, the optical, thermal and electrical design of the LED luminaires must be considered differently. Thus, this study concentrates upon design considerations and the operating principle of solarpowered LED road lighting luminaire in details. Also, a simple solar panel system was designed and the economical values obtained at the end of 20 years were compared when using the ongrid system and the off-grid system.

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Guidelines for principal component reliability testing for LED light sources and LED luminaires

10.3403/30323174u ◽

2017 ◽

Keyword(s):

Principal Component ◽

Light Sources ◽

Reliability Testing ◽

Component Reliability ◽

Led Light

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Faculty Opinions recommendation of Cross-talk between singlet oxygen- and hydrogen peroxide-dependent signaling of stress responses in Arabidopsis thaliana.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1064734.517650 ◽

2007 ◽

Author(s):

Eric Lam

Keyword(s):

Hydrogen Peroxide ◽

Arabidopsis Thaliana ◽

Singlet Oxygen ◽

Cross Talk ◽

Stress Responses

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Biomass growth of red spinach in plant-factory system under three kinds of LED light sources

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/653/1/012100 ◽

2021 ◽

Vol 653 (1) ◽

pp. 012100

Author(s):

Ardiansyah ◽

A Hardanto ◽

E Sumarni

Keyword(s):

Light Sources ◽

Biomass Growth ◽

Led Light ◽

Plant Factory

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Alternative splicing landscapes in Arabidopsis thaliana across tissues and stress conditions highlight major functional differences with animals

Genome Biology ◽

10.1186/s13059-020-02258-y ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Guiomar Martín ◽

Yamile Márquez ◽

Federica Mantica ◽

Paula Duque ◽

Manuel Irimia

Keyword(s):

Gene Expression ◽

Arabidopsis Thaliana ◽

Alternative Splicing ◽

Stress Responses ◽

Target Genes ◽

Intron Retention ◽

Single Gene ◽

Exon Skipping ◽

Environmental Response ◽

Biotic Stresses

Abstract Background Alternative splicing (AS) is a widespread regulatory mechanism in multicellular organisms. Numerous transcriptomic and single-gene studies in plants have investigated AS in response to specific conditions, especially environmental stress, unveiling substantial amounts of intron retention that modulate gene expression. However, a comprehensive study contrasting stress-response and tissue-specific AS patterns and directly comparing them with those of animal models is still missing. Results We generate a massive resource for Arabidopsis thaliana, PastDB, comprising AS and gene expression quantifications across tissues, development and environmental conditions, including abiotic and biotic stresses. Harmonized analysis of these datasets reveals that A. thaliana shows high levels of AS, similar to fruitflies, and that, compared to animals, disproportionately uses AS for stress responses. We identify core sets of genes regulated specifically by either AS or transcription upon stresses or among tissues, a regulatory specialization that is tightly mirrored by the genomic features of these genes. Unexpectedly, non-intron retention events, including exon skipping, are overrepresented across regulated AS sets in A. thaliana, being also largely involved in modulating gene expression through NMD and uORF inclusion. Conclusions Non-intron retention events have likely been functionally underrated in plants. AS constitutes a distinct regulatory layer controlling gene expression upon internal and external stimuli whose target genes and master regulators are hardwired at the genomic level to specifically undergo post-transcriptional regulation. Given the higher relevance of AS in the response to different stresses when compared to animals, this molecular hardwiring is likely required for a proper environmental response in A. thaliana.

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Analysis on High-Power Seaport LED Luminaire with Uniform Light Distribution Based on Optimized Lens and Heatsink

Applied Sciences ◽

10.3390/app11094035 ◽

2021 ◽

Vol 11 (9) ◽

pp. 4035

Author(s):

Jinsheon Kim ◽

Jeungmo Kang ◽

Woojin Jang

Keyword(s):

Light Source ◽

High Power ◽

Heat Dissipation ◽

Light Emitting Diode ◽

Light Distribution ◽

Light Sources ◽

Led Light ◽

High Power Led ◽

Distribution Requirement ◽

Lighting Application

In the case of light-emitting diode (LED) seaport luminaires, they should be designed in consideration of glare, average illuminance, and overall uniformity. Although it is possible to implement light distribution through auxiliary devices such as reflectors, it means increasing the weight and size of the luminaire, which reduces the feasibility. Considering the special environment of seaport luminaires, which are installed at a height of 30 m or more, it is necessary to reduce the weight of the device, facilitate replacement, and secure a light source with a long life. In this paper, an optimized lens design was investigated to provide uniform light distribution to meet the requirement in the seaport lighting application. Four types of lens were designed and fabricated to verify the uniform light distribution requirement for the seaport lighting application. Using numerical analysis, we optimized the lens that provides the required minimum overall uniformity for the seaport lighting application. A theoretical analysis for the heatsink structure and shape were conducted to reduce the heat from the high-power LED light sources up to 250 W. As a result of these analyses on the heat dissipation characteristics of the high-power LED light source used in the LED seaport luminaire, the heatsink with hexagonal-shape fins shows the best heat dissipation effect. Finally, a prototype LED seaport luminaire with an optimized lens and heat sink was fabricated and tested in a real seaport environment. The light distribution characteristics of this prototype LED seaport luminaire were compared with a commercial high-pressure sodium luminaire and metal halide luminaire.

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An Efficient Method for LED Light Sources Characterization

Electronics ◽

10.3390/electronics8101089 ◽

2019 ◽

Vol 8 (10) ◽

pp. 1089 ◽

Cited By ~ 2

Author(s):

Sara Raggiunto ◽

Alberto Belli ◽

Lorenzo Palma ◽

Piergiovanni Ceregioli ◽

Massimo Gattari ◽

...

Keyword(s):

Efficient Method ◽

Least Squares Method ◽

Color Temperature ◽

Experimental Setup ◽

Light Sources ◽

Careful Study ◽

Source Characterization ◽

Led Light ◽

Lighting Systems ◽

Led Drivers

Digital LED drivers capable of blending the spectrum of two LED fixtures with different Correlated Color Temperatures through the LEDs’ power supply control are widespread. However, the digital control of lighting systems is possible only after a careful study of the LED’s response, in terms of illuminance and Correlated Color Temperature. The proposed work takes advantage of the Tunable White technology for the realization of an efficient method for LED light source characterization. In order to evaluate how the light changes as a function of the electric power supplied by the LED drivers, an experimental setup to characterize LED light sources has been designed. Starting from the data acquired from the experimental setup, a model for dimming the LED driver and obtaining the desired values of quality of light has been developed. The proposed model is based on the least squares method and its accuracy is evaluated by comparing the obtained values of illuminance and Correlated Color Temperature with those measured by an illuminance spectrophotometer. Results achieved an error of 0.3% for Correlated Color Temperature and 1.5% for illuminance using the proposed approximation functions.

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